BACKGROUND： Ultrashortwave （USW） therapy may be a new method for treatment of ischemic cerebrovascular diseases. It is necessary to study its treatment time window. OBJECTIVE： To observe the effect of USW on reper...BACKGROUND： Ultrashortwave （USW） therapy may be a new method for treatment of ischemic cerebrovascular diseases. It is necessary to study its treatment time window. OBJECTIVE： To observe the effect of USW on reperfusion injury after occlusion of the middle cerebral artery （MCAO） in rats and discuss its acting mechanisms and best occasion. DESIGN： Randomized controlled observation, animal experiment. SETTING： Laboratory of Department of Rehabilitation Medicine, First Hospital Affiliated to China Medical University. MATERIALS： Sixty-six healthy Wistar rats of either gender and of clean grade, aged 18–20 weeks, weighing from 250 to 300 g, were provided by the Experimental Animal Center of China Medical University. An USW device （Shanghai Electrical Device Company） with the frequency of 40.68 MHz and the maximum output power of 40 W, and the first channel power controlled at about 11 W was used in this study. Output power was determined by photometry. METHODS： Sixty-six rats were randomly divided into 3 groups： Sham-operation group （n =6）： The suture was inserted only 1.0 depth during operation, which did not cause MACO; Model group （n =12）： The USW treatment procedure was performed with the power off on the model rats; USW treatment group （n =48）： The 48 rats were randomly divided into modeling 0, 6, 12 and 18 hours 4 subgroups. USW therapy without heat was used on the head of rats for 10 minutes at each time point. Twelve rats in USW treatment group were decapitated following treatment at each time point, and then their brain tissues were harvested. The rat brain tissues in other groups were harvested by decapitation at 24 hours after modeling. When the rats were awake, the neurologic deficit was scored by Zea-Longa five-point scale （a score of 0 indicated no neurologic deficit, a score of 1 indicated failure to extend left paw fully, a score of 2 indicated circling to the left, and a score of 3 indicated falling to the left, and rats with a score of 4 did not walk spontaneously and has a depressed level of consciousness.） Rats which still survived at 24 hours and was scored 1 and 2 on the neurologic scoring were involved in the analysis. ① Determination of cerebral water content： Cerebral water contents of healthy and injured hemisphere were determined by wet/dry weighing method. Cerebral water content （100%） =（1–dry/wet weight）×100%.②Infarction volume： The brain tissue was sliced into 2 mm sections and each section was stained with 20 g/L 2,3,5-triphenyltetrazolium chloride （TTC） by TTC staining technique for 30 minutes in a water bath at 37 ℃.Then, the section was fixed in 100 g/L formaldehyde for 10 minutes .The infarction volume was analyzed by using an imaging analyzer.③ Preparation of light microscopic sample： The rat brain tissue fixed by 100 g/L neutral formaldehyde and stained with TTC, were gradiently dehydrated with alcoholic, embedded with paraffin, sliced and stained by HE, finally, the sections were observed under the light microscope. MAIN OUTCOME MEASURES： Cerebral water content, cerebral infarction volume and cerebral histomorphology of rats in each group. RESULTS： Sixty-six rats were involved in the final analysis. ①Cerebral water content： There were no significant differences of cerebral water content in healthy hemisphere among groups （P 〉 0.05）. Cerebral water content of injured hemisphere in the model group and at modeling 0, 6, 12 and 18 hours in the USW treatment group was （81.50±0.74） %, （81.02±0.83） %, （79.78±0.70） %, （79.74±0.84） %, （79.39± 1.06） %, respectively, which was significantly higher than that in the sham-operation group [（78.09±0.52） %, P 〈 0.05]. At modeling 0, 6 and 12 hours, the cerebral water content in the injured hemisphere in the USW treatment group was significantly lower than that in the model group, respectively （P 〈 0.05）. It indicatedthat USW treatment given at 6, 12 and 18 hours after ischemia/reperfusion can lessen brain edema. ② Cerebral infarction volume： At modeling 18 hours, cerebral infarction volume in the injured hemisphere of USW treatment group was smaller than that in the model group [（191.62±121.45）,（362.03±142.01）mm3, t =2.23,P 〈 0.05]. ③ Cerebral histomorphological observation： No swelling was found in the brain tissue section of rats in the sham-operation group. In the model group, the size of infarction hemisphere was obviously increased, gyrus became flattened, cortical sulci was shallow, the color at infarct focus obviously became light, and the tissue was fragile and brittle. In the sham-operation group, it was found under the microscope that mesenchyma was highly swelled, neuronal peripheral interspace was obviously broadened, neurons presented triangle, nucleoli were reduced, condensed even disappeared, and neutrophils in the vascular cavity were obviously increased. In the USW treatment group, pathological injury was not obviously lessened at 0 hour, moderate or mild edema could be found in the injured hemisphere of USW treatment group at modeling 6,12 and 18 hours, and at this time, neutrophils in vascular cavity were increased slightly, and pathological injuries were lessened. CONCLUSION： USW may play a protective effect on cerebral ischemia/reperfusion injury by decreasing brain edema and/or cerebral infarction volume. The treatment action of USW may start at 6 hours after reperfusion, and the best occasion of application may be at 18 hours after reperfusion.展开更多
OBJECTIVE: To evaluate the pain scores and rehabilitation of patientis with cervical spondylotic radiculopathy receiving massage therapy combined with traction and ultrashort wave therapy. METHODS: A total of 84 patie...OBJECTIVE: To evaluate the pain scores and rehabilitation of patientis with cervical spondylotic radiculopathy receiving massage therapy combined with traction and ultrashort wave therapy. METHODS: A total of 84 patients with cervical spondylotic radiculopathy treated in 105 Hospital of People's Liberation Army from June 2014 to June 2017 were included and divided into study group and control group according to different treatment regimens. A total of 42 patients in the study group was treated with cervical traction, ultrashort wave and massage therapy at the same time, whereas the other 42 patients in the control group were only treated with cervical traction and ultrashort wave. Comparison of the clinical efficacy and adverse reactions of the 2 groups was made and the Visual Analogue Scale (VAS) scores before and after treatment were observed to assess the patient's pain. Comparison of rehabilitation in the 2 groups was made by applying the Clinical Assessment Scale for Cervical Spondylosis (CASCS). RESULTS: After comparing the therapeutic effect between the study group and the control group, it showed that the total effective rate (90.48%) in the study group was significantly higher than that in the control group (73.81%), the difference was statistically significant (P < 0.05). By comparing the incidence of adverse reactions in the study group and the control group, results showed that the incidence of adverse reactions such as nausea, palpitations, vomiting, sweating, dizziness and colorless complexion in the study group (9.52%) was significantly lower than that in the control group (21.43%), the difference was statistically significant (P < 0.05). After the treatment, the VAS pain scores of the study group and the control group were both significantly improved (P < 0.05), but the improvement of the patients in the study group was more significantly (P < 0.05), and the difference was statistically significant. After treatment, the CASCS scores of the patients in the study group and the control group were significantly improved (P < 0.05), but the improvement of the study group was more (P < 0.05), and there was statistically significant difference. CONCLUSION: The combination of massage, traction and ultrashort wave therapy can significantly reduce the pain and reduce the incidence of adverse reactions in patients with cervical spondylotic radiculopathy, which is of great clinical significance to the rehabilitation of patients.展开更多
Aim:The present study aimed to assess the clinical effects of ultrashort wave therapy combined with acupuncture and rehabilitation training on patients with dysphagia after stroke.Method:A total of 126 patients with s...Aim:The present study aimed to assess the clinical effects of ultrashort wave therapy combined with acupuncture and rehabilitation training on patients with dysphagia after stroke.Method:A total of 126 patients with stroke with dysphagia were randomly divided into an acupuncture group(control group:63 patients)and a comprehensive rehabilitation training group(treatment group:63 patients).The control group received rehabilitation training and acupuncture,whereas the treatment group received ultrashort wave therapy in addition to rehabilitation training and acupuncture(comprehensive rehabilitation training).The curative effect was evaluated using water-drinking test scores and swallowing quality of life scale(SWAL-QOL)scores before and after intervention.Additionally,the incidence of aspiration pneumonia was assessed in the two groups.Results:The water-drinking test scores in both groups were significantly better after 4 weeks of intervention than before intervention(P<0.01);however,the improvement degree was significantly greater in the treat-ment group than in the control group(P<0.01).The SWAL-QOL scores in both groups were significantly higher after intervention than before intervention(P<0.05);however,the improvement degree was significantly greater in the treatment group than in the control group(P<0.05).Moreover,the incidence of aspiration pneumonia was significantly lower in the treatment group than in the control group(P<0.05).Conclusion:Comprehensive rehabilitation training can greatly improve dysphagia after stroke and can effectively reduce the incidence of aspiration pneumonia.展开更多
Background:Ultrashort wave(USW)therapy has been reported to alleviate cerebral ischemia/reperfusion(IR)injury,however the underlying mechanisms remain elusive.The aim of this study was to observe the effect of non-the...Background:Ultrashort wave(USW)therapy has been reported to alleviate cerebral ischemia/reperfusion(IR)injury,however the underlying mechanisms remain elusive.The aim of this study was to observe the effect of non-thermal USW therapy on neuronal damage and expression of heat shock protein 70(HSP70)after cerebral IR in rats.Methods:Focal ischemia-reperfusion(IR)was induced in SpragueeDawley rats by middle cerebral artery occlusion/reperfusion(MCAO/R).The Ninety-two rats(both male and female)were screened using the Zea-Longa 5 grade evaluation.Included rats were then randomly divided into blank,sham,model 1-day,model 3-day,model 7-day,USW 1-day,USW 3-day,or USW 7-day groups.All rats in the model groups received sham USW treatment,while rats in the USW groups received USWtreatment,for 1,3,or 7 days.We assessed the National Institutes of Health Stroke Scale,brain infarction volumes,ultrastructural damage scores using electron microscopy,and HSP70 expression by western blotting between the different groups.Results:USW treatment reduced the National Institutes of Health Stroke Scale,infarction volume,and ultrastructural neuronal damage,and increased expression of HSP70,in the hippocampal CA1 region.Conclusions:Non-thermal USW therapy may improve neurological function,decrease infarction volume,and reduce neuronal damage by increasing HSP70 expression following cerebral IR injury.展开更多
Based on a modified coupled wave theory of Kogelnik, we have studied the diffraction of an ultrashort pulsed beam with an arbitrary polarization state from a volume holographic grating in photorefractive LiNbO3 crysta...Based on a modified coupled wave theory of Kogelnik, we have studied the diffraction of an ultrashort pulsed beam with an arbitrary polarization state from a volume holographic grating in photorefractive LiNbO3 crystals. The results indicate that the diffracted intensity distributions in the spectral and temporal domains and the diffraction efficiency of the grating are both changed by the polarization state and spectral bandwidth of the input pulsed beam. A method is given of choosing the grating parameters and input conditions to obtain a large variation range of the spectral bandwidth of the diffracted pulsed beam with an appropriate diffraction efficiency. Our study presents a possibility of using a volume holographic grating recorded in anisotropic materials to shape a broadband ultrashort pulsed beam by modulating its polarization state.展开更多
目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处...目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处理组根据处理时长不同分为5组:30 s组、1 min组、3 min组、5 min组和7 min组,每天按照分组处理1次,通过CCK-8和qRT-PCR检测细胞增殖;Western blot、qRT-PCR检测凋亡相关标志物Bax、Bcl-2的表达,成骨相关标志物Runx2、ALP的表达。结果CCK-8结果显示,3 min USW处理JBMSCs可显著增强其第3天和第5天的OD值,3 min USW可上调Bcl-2的蛋白和mRNA表达水平,下调Bax的蛋白和mRNA表达水平;此外,3 min USW可有效上调ALP、Runx2的蛋白和mRNA表达水平。结论3 min USW处理能有效提高JBMSCs增殖和成骨分化能力并抑制其凋亡。展开更多
文摘BACKGROUND： Ultrashortwave （USW） therapy may be a new method for treatment of ischemic cerebrovascular diseases. It is necessary to study its treatment time window. OBJECTIVE： To observe the effect of USW on reperfusion injury after occlusion of the middle cerebral artery （MCAO） in rats and discuss its acting mechanisms and best occasion. DESIGN： Randomized controlled observation, animal experiment. SETTING： Laboratory of Department of Rehabilitation Medicine, First Hospital Affiliated to China Medical University. MATERIALS： Sixty-six healthy Wistar rats of either gender and of clean grade, aged 18–20 weeks, weighing from 250 to 300 g, were provided by the Experimental Animal Center of China Medical University. An USW device （Shanghai Electrical Device Company） with the frequency of 40.68 MHz and the maximum output power of 40 W, and the first channel power controlled at about 11 W was used in this study. Output power was determined by photometry. METHODS： Sixty-six rats were randomly divided into 3 groups： Sham-operation group （n =6）： The suture was inserted only 1.0 depth during operation, which did not cause MACO; Model group （n =12）： The USW treatment procedure was performed with the power off on the model rats; USW treatment group （n =48）： The 48 rats were randomly divided into modeling 0, 6, 12 and 18 hours 4 subgroups. USW therapy without heat was used on the head of rats for 10 minutes at each time point. Twelve rats in USW treatment group were decapitated following treatment at each time point, and then their brain tissues were harvested. The rat brain tissues in other groups were harvested by decapitation at 24 hours after modeling. When the rats were awake, the neurologic deficit was scored by Zea-Longa five-point scale （a score of 0 indicated no neurologic deficit, a score of 1 indicated failure to extend left paw fully, a score of 2 indicated circling to the left, and a score of 3 indicated falling to the left, and rats with a score of 4 did not walk spontaneously and has a depressed level of consciousness.） Rats which still survived at 24 hours and was scored 1 and 2 on the neurologic scoring were involved in the analysis. ① Determination of cerebral water content： Cerebral water contents of healthy and injured hemisphere were determined by wet/dry weighing method. Cerebral water content （100%） =（1–dry/wet weight）×100%.②Infarction volume： The brain tissue was sliced into 2 mm sections and each section was stained with 20 g/L 2,3,5-triphenyltetrazolium chloride （TTC） by TTC staining technique for 30 minutes in a water bath at 37 ℃.Then, the section was fixed in 100 g/L formaldehyde for 10 minutes .The infarction volume was analyzed by using an imaging analyzer.③ Preparation of light microscopic sample： The rat brain tissue fixed by 100 g/L neutral formaldehyde and stained with TTC, were gradiently dehydrated with alcoholic, embedded with paraffin, sliced and stained by HE, finally, the sections were observed under the light microscope. MAIN OUTCOME MEASURES： Cerebral water content, cerebral infarction volume and cerebral histomorphology of rats in each group. RESULTS： Sixty-six rats were involved in the final analysis. ①Cerebral water content： There were no significant differences of cerebral water content in healthy hemisphere among groups （P 〉 0.05）. Cerebral water content of injured hemisphere in the model group and at modeling 0, 6, 12 and 18 hours in the USW treatment group was （81.50±0.74） %, （81.02±0.83） %, （79.78±0.70） %, （79.74±0.84） %, （79.39± 1.06） %, respectively, which was significantly higher than that in the sham-operation group [（78.09±0.52） %, P 〈 0.05]. At modeling 0, 6 and 12 hours, the cerebral water content in the injured hemisphere in the USW treatment group was significantly lower than that in the model group, respectively （P 〈 0.05）. It indicatedthat USW treatment given at 6, 12 and 18 hours after ischemia/reperfusion can lessen brain edema. ② Cerebral infarction volume： At modeling 18 hours, cerebral infarction volume in the injured hemisphere of USW treatment group was smaller than that in the model group [（191.62±121.45）,（362.03±142.01）mm3, t =2.23,P 〈 0.05]. ③ Cerebral histomorphological observation： No swelling was found in the brain tissue section of rats in the sham-operation group. In the model group, the size of infarction hemisphere was obviously increased, gyrus became flattened, cortical sulci was shallow, the color at infarct focus obviously became light, and the tissue was fragile and brittle. In the sham-operation group, it was found under the microscope that mesenchyma was highly swelled, neuronal peripheral interspace was obviously broadened, neurons presented triangle, nucleoli were reduced, condensed even disappeared, and neutrophils in the vascular cavity were obviously increased. In the USW treatment group, pathological injury was not obviously lessened at 0 hour, moderate or mild edema could be found in the injured hemisphere of USW treatment group at modeling 6,12 and 18 hours, and at this time, neutrophils in vascular cavity were increased slightly, and pathological injuries were lessened. CONCLUSION： USW may play a protective effect on cerebral ischemia/reperfusion injury by decreasing brain edema and/or cerebral infarction volume. The treatment action of USW may start at 6 hours after reperfusion, and the best occasion of application may be at 18 hours after reperfusion.
文摘OBJECTIVE: To evaluate the pain scores and rehabilitation of patientis with cervical spondylotic radiculopathy receiving massage therapy combined with traction and ultrashort wave therapy. METHODS: A total of 84 patients with cervical spondylotic radiculopathy treated in 105 Hospital of People's Liberation Army from June 2014 to June 2017 were included and divided into study group and control group according to different treatment regimens. A total of 42 patients in the study group was treated with cervical traction, ultrashort wave and massage therapy at the same time, whereas the other 42 patients in the control group were only treated with cervical traction and ultrashort wave. Comparison of the clinical efficacy and adverse reactions of the 2 groups was made and the Visual Analogue Scale (VAS) scores before and after treatment were observed to assess the patient's pain. Comparison of rehabilitation in the 2 groups was made by applying the Clinical Assessment Scale for Cervical Spondylosis (CASCS). RESULTS: After comparing the therapeutic effect between the study group and the control group, it showed that the total effective rate (90.48%) in the study group was significantly higher than that in the control group (73.81%), the difference was statistically significant (P < 0.05). By comparing the incidence of adverse reactions in the study group and the control group, results showed that the incidence of adverse reactions such as nausea, palpitations, vomiting, sweating, dizziness and colorless complexion in the study group (9.52%) was significantly lower than that in the control group (21.43%), the difference was statistically significant (P < 0.05). After the treatment, the VAS pain scores of the study group and the control group were both significantly improved (P < 0.05), but the improvement of the patients in the study group was more significantly (P < 0.05), and the difference was statistically significant. After treatment, the CASCS scores of the patients in the study group and the control group were significantly improved (P < 0.05), but the improvement of the study group was more (P < 0.05), and there was statistically significant difference. CONCLUSION: The combination of massage, traction and ultrashort wave therapy can significantly reduce the pain and reduce the incidence of adverse reactions in patients with cervical spondylotic radiculopathy, which is of great clinical significance to the rehabilitation of patients.
文摘Aim:The present study aimed to assess the clinical effects of ultrashort wave therapy combined with acupuncture and rehabilitation training on patients with dysphagia after stroke.Method:A total of 126 patients with stroke with dysphagia were randomly divided into an acupuncture group(control group:63 patients)and a comprehensive rehabilitation training group(treatment group:63 patients).The control group received rehabilitation training and acupuncture,whereas the treatment group received ultrashort wave therapy in addition to rehabilitation training and acupuncture(comprehensive rehabilitation training).The curative effect was evaluated using water-drinking test scores and swallowing quality of life scale(SWAL-QOL)scores before and after intervention.Additionally,the incidence of aspiration pneumonia was assessed in the two groups.Results:The water-drinking test scores in both groups were significantly better after 4 weeks of intervention than before intervention(P<0.01);however,the improvement degree was significantly greater in the treat-ment group than in the control group(P<0.01).The SWAL-QOL scores in both groups were significantly higher after intervention than before intervention(P<0.05);however,the improvement degree was significantly greater in the treatment group than in the control group(P<0.05).Moreover,the incidence of aspiration pneumonia was significantly lower in the treatment group than in the control group(P<0.05).Conclusion:Comprehensive rehabilitation training can greatly improve dysphagia after stroke and can effectively reduce the incidence of aspiration pneumonia.
基金granted by the National Natural Science Foundation of China(No.81971098).
文摘Background:Ultrashort wave(USW)therapy has been reported to alleviate cerebral ischemia/reperfusion(IR)injury,however the underlying mechanisms remain elusive.The aim of this study was to observe the effect of non-thermal USW therapy on neuronal damage and expression of heat shock protein 70(HSP70)after cerebral IR in rats.Methods:Focal ischemia-reperfusion(IR)was induced in SpragueeDawley rats by middle cerebral artery occlusion/reperfusion(MCAO/R).The Ninety-two rats(both male and female)were screened using the Zea-Longa 5 grade evaluation.Included rats were then randomly divided into blank,sham,model 1-day,model 3-day,model 7-day,USW 1-day,USW 3-day,or USW 7-day groups.All rats in the model groups received sham USW treatment,while rats in the USW groups received USWtreatment,for 1,3,or 7 days.We assessed the National Institutes of Health Stroke Scale,brain infarction volumes,ultrastructural damage scores using electron microscopy,and HSP70 expression by western blotting between the different groups.Results:USW treatment reduced the National Institutes of Health Stroke Scale,infarction volume,and ultrastructural neuronal damage,and increased expression of HSP70,in the hippocampal CA1 region.Conclusions:Non-thermal USW therapy may improve neurological function,decrease infarction volume,and reduce neuronal damage by increasing HSP70 expression following cerebral IR injury.
基金Project supported by the State Key Development Program for Basic Research of China （Grant No 2002CCA03500）, and the National Natural Science Foundation of China （Grant No 60177016）.
文摘Based on a modified coupled wave theory of Kogelnik, we have studied the diffraction of an ultrashort pulsed beam with an arbitrary polarization state from a volume holographic grating in photorefractive LiNbO3 crystals. The results indicate that the diffracted intensity distributions in the spectral and temporal domains and the diffraction efficiency of the grating are both changed by the polarization state and spectral bandwidth of the input pulsed beam. A method is given of choosing the grating parameters and input conditions to obtain a large variation range of the spectral bandwidth of the diffracted pulsed beam with an appropriate diffraction efficiency. Our study presents a possibility of using a volume holographic grating recorded in anisotropic materials to shape a broadband ultrashort pulsed beam by modulating its polarization state.
文摘目的探究超短波对颌骨骨髓间充质干细胞(JBMSCs)增殖、凋亡和成骨分化特性的影响。方法原代提取培养C57BL/6N小鼠JBMSCs并鉴定。取第三代JBMSCs进行实验,将细胞接种于培养板并随机分为非超短波处理组(Con)和超短波处理组(USW),超短波处理组根据处理时长不同分为5组:30 s组、1 min组、3 min组、5 min组和7 min组,每天按照分组处理1次,通过CCK-8和qRT-PCR检测细胞增殖;Western blot、qRT-PCR检测凋亡相关标志物Bax、Bcl-2的表达,成骨相关标志物Runx2、ALP的表达。结果CCK-8结果显示,3 min USW处理JBMSCs可显著增强其第3天和第5天的OD值,3 min USW可上调Bcl-2的蛋白和mRNA表达水平,下调Bax的蛋白和mRNA表达水平;此外,3 min USW可有效上调ALP、Runx2的蛋白和mRNA表达水平。结论3 min USW处理能有效提高JBMSCs增殖和成骨分化能力并抑制其凋亡。
