The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrat...The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrate that OsCSLC3,a member of the rice cellulose synthase-like(CSL)gene family,is predominantly expressed in vascular tissues and that its overexpression promotes hemicellulose biosynthesis.This enhancement of hemicellulose accumulation is associated with improved disease resistance.Targeted editing of conserved cis-regulatory elements in the OsCSLC35′untranslated region(UTR)showed that deletion of the specific fragment(−575 to−824 bp)elevated OsCSLC3 transcript levels,promoted hemicellulose accumulation,enhanced disease resistance,and improved agronomic traits.Our findings highlight a previously underappreciated role for hemicellulose in plant immunity and demonstrate that precise 5′UTR editing is a promising strategy for improving disease resistance and agronomic traits.展开更多
基金supported by the Major Projects in Agricultural Biological Breeding(2022ZD04002)to Xuewei ChenNational Natural Science Foundation of China(32121003 and 32425005)to Xuewei Chen+3 种基金(32172419 and 32372555)to Weitao LiCornerstone Science Foundation through the XPLORER PRIZE and New Cornerstone Investigator Program to Xuewei ChenSichuan Science and Technology Program(2023NSFSC0005)to Xuewei Chen,(2023NSFSC1996)to Weitao Li,(2023NSFSC0155)to Qingqing Hou(2024YFNH0014)to Junjie Yin.
文摘The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrate that OsCSLC3,a member of the rice cellulose synthase-like(CSL)gene family,is predominantly expressed in vascular tissues and that its overexpression promotes hemicellulose biosynthesis.This enhancement of hemicellulose accumulation is associated with improved disease resistance.Targeted editing of conserved cis-regulatory elements in the OsCSLC35′untranslated region(UTR)showed that deletion of the specific fragment(−575 to−824 bp)elevated OsCSLC3 transcript levels,promoted hemicellulose accumulation,enhanced disease resistance,and improved agronomic traits.Our findings highlight a previously underappreciated role for hemicellulose in plant immunity and demonstrate that precise 5′UTR editing is a promising strategy for improving disease resistance and agronomic traits.
