The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrat...The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrate that OsCSLC3,a member of the rice cellulose synthase-like(CSL)gene family,is predominantly expressed in vascular tissues and that its overexpression promotes hemicellulose biosynthesis.This enhancement of hemicellulose accumulation is associated with improved disease resistance.Targeted editing of conserved cis-regulatory elements in the OsCSLC35′untranslated region(UTR)showed that deletion of the specific fragment(−575 to−824 bp)elevated OsCSLC3 transcript levels,promoted hemicellulose accumulation,enhanced disease resistance,and improved agronomic traits.Our findings highlight a previously underappreciated role for hemicellulose in plant immunity and demonstrate that precise 5′UTR editing is a promising strategy for improving disease resistance and agronomic traits.展开更多
2025年9月26日,北京大学药学院天然药物及仿生药物全国重点实验室汤新景教授团队联合王静博士在国际学术期刊Journal of Medicinal Chemistry发表了题为“Efficient Silencing of Androgen Receptor Gene via UTR-TargetingsiRNAs for A...2025年9月26日,北京大学药学院天然药物及仿生药物全国重点实验室汤新景教授团队联合王静博士在国际学术期刊Journal of Medicinal Chemistry发表了题为“Efficient Silencing of Androgen Receptor Gene via UTR-TargetingsiRNAs for Androgenetic Alopecia Therapy”的研究论文。该研究首次证实靶向雄激素受体基因非编码区的siRNA在雄激素性脱发治疗中的优势,为开发下一代脱发基因疗法提供了有力依据。展开更多
钙蛋白酶抑制蛋白(Calpastatin,CAST)专一抑制钙蛋白酶(Calpain,CAPN)活性,参与调节牛肉的嫩化及肌内蛋白的水解,其编码基因是肉质性状的主要候选基因。本研究根据牛CAST基因5'UTR区(GenBank No.AY834771)和3'UTR区(Gen Bank No...钙蛋白酶抑制蛋白(Calpastatin,CAST)专一抑制钙蛋白酶(Calpain,CAPN)活性,参与调节牛肉的嫩化及肌内蛋白的水解,其编码基因是肉质性状的主要候选基因。本研究根据牛CAST基因5'UTR区(GenBank No.AY834771)和3'UTR区(Gen Bank No.DQ991097)序列设计两对引物,用PCR-SSCP方法对鲁西黄牛和渤海黑牛共305个样本进行了单核苷酸多态性分析。结果显示,CAST基因5'UTR和3'UTR区存在PCR-SSCP多态,在5'UTR区存在6437(C/T)、6477(G/A)和6614(G/T)3个SNP位点,表现为AA、AB、BB、BC和AD 5种基因型;在3'UTR区存在359(T/C)和366(A/G)2个SNP位点,表现为EE、EF、FF和EG 4种基因型。经检验表明,鲁西黄牛和渤海黑牛均处于非Hardy-Weinberg平衡状态;群体遗传多态性分析表明这两种牛的多态信息含量均为中度多态。展开更多
基金supported by the Major Projects in Agricultural Biological Breeding(2022ZD04002)to Xuewei ChenNational Natural Science Foundation of China(32121003 and 32425005)to Xuewei Chen+3 种基金(32172419 and 32372555)to Weitao LiCornerstone Science Foundation through the XPLORER PRIZE and New Cornerstone Investigator Program to Xuewei ChenSichuan Science and Technology Program(2023NSFSC0005)to Xuewei Chen,(2023NSFSC1996)to Weitao Li,(2023NSFSC0155)to Qingqing Hou(2024YFNH0014)to Junjie Yin.
文摘The plant cell wall serves as a barrier in defense against pathogen invasion.However,the specific contribution of cell walls in vascular tissues to plant immunity remains largely unexplored.In this study,we demonstrate that OsCSLC3,a member of the rice cellulose synthase-like(CSL)gene family,is predominantly expressed in vascular tissues and that its overexpression promotes hemicellulose biosynthesis.This enhancement of hemicellulose accumulation is associated with improved disease resistance.Targeted editing of conserved cis-regulatory elements in the OsCSLC35′untranslated region(UTR)showed that deletion of the specific fragment(−575 to−824 bp)elevated OsCSLC3 transcript levels,promoted hemicellulose accumulation,enhanced disease resistance,and improved agronomic traits.Our findings highlight a previously underappreciated role for hemicellulose in plant immunity and demonstrate that precise 5′UTR editing is a promising strategy for improving disease resistance and agronomic traits.
文摘2025年9月26日,北京大学药学院天然药物及仿生药物全国重点实验室汤新景教授团队联合王静博士在国际学术期刊Journal of Medicinal Chemistry发表了题为“Efficient Silencing of Androgen Receptor Gene via UTR-TargetingsiRNAs for Androgenetic Alopecia Therapy”的研究论文。该研究首次证实靶向雄激素受体基因非编码区的siRNA在雄激素性脱发治疗中的优势,为开发下一代脱发基因疗法提供了有力依据。
