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基于UPLC-Q-TOF-MS技术、网络药理学、分子对接探讨索罗西汤对细菌性肺炎的作用机制
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作者 罗俊聪 白玛卓玛 +5 位作者 叶芬 乔玉凤 李建平 吾坚 邸学 王添敏 《山东化工》 2026年第3期21-27,共7页
目的:基于UPLC-Q-TOF-MS(超高效液相色谱—飞行时间质谱联用技术)、网络药理学及分子对接探讨索罗西汤治疗细菌性肺炎的药效物质及潜在机制。方法:通过UPLC-Q-TOF-MS分析并鉴定索罗西汤的化学成分;通过网络药理学方法分析索罗西汤的活... 目的:基于UPLC-Q-TOF-MS(超高效液相色谱—飞行时间质谱联用技术)、网络药理学及分子对接探讨索罗西汤治疗细菌性肺炎的药效物质及潜在机制。方法:通过UPLC-Q-TOF-MS分析并鉴定索罗西汤的化学成分;通过网络药理学方法分析索罗西汤的活性成分、作用靶点及信号通路;采用分子对接技术分析活性成分与作用靶点的结合情况。结果:共鉴定82种化学成分,通过HERB和SwissADME数据库鉴定出13种活性成分。与BP的交集靶点109个,取degree值前十的核心靶点进行GO、KEGG富集。GO富集分析显示共有575条信号通路,其中包括酶结合、膜筏、脂多糖诱导的细胞反应等关键途径;KEGG富集分析显示共有147条信号通路,其中包括脂质与动脉粥样硬化、AGE-RAGE、TNF信号通路等关键途径。分子对接结果表明脱落酸、羟基阿魏酸等活性成分与关键靶点TNF、STAT3和AKT1均具有较好的结合能力。结论:通过UPLC-Q-TOF-MS结合网络药理学、分子对接,初步阐释了索罗西汤对细菌性肺炎的潜在通路及机制,为索罗西汤的深入开发及应用提供了理论依据。 展开更多
关键词 索罗西汤 细菌性肺炎 uplc-q-tof-ms 网络药理学 分子对接
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UPLC-Q-TOF-MS/MS法鉴定3种酚类在小鼠体内的代谢产物
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作者 赵蓉 胡蒙蒙 +3 位作者 洪玉冰 雷胜男 赵子康 车彦云 《中成药》 北大核心 2026年第1期33-41,共9页
目的建立UPLC-Q-TOF-MS/MS法鉴定没食子酸、鞣花酸、柯里拉京在小鼠体内的代谢产物。方法给药组小鼠分别灌胃给予3种成分的0.5%CMC-Na混悬液(400 mg/kg),空白组小鼠灌胃给予0.5%CMC-Na混悬液(400 mg/kg),采集血浆、尿液、粪便。分析采用... 目的建立UPLC-Q-TOF-MS/MS法鉴定没食子酸、鞣花酸、柯里拉京在小鼠体内的代谢产物。方法给药组小鼠分别灌胃给予3种成分的0.5%CMC-Na混悬液(400 mg/kg),空白组小鼠灌胃给予0.5%CMC-Na混悬液(400 mg/kg),采集血浆、尿液、粪便。分析采用Acquity UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm);流动相水(含0.1%甲酸)-乙腈,梯度洗脱;体积流量0.27 mL/min;柱温30℃;电喷雾离子源;负离子扫描。结果共鉴定出27种没食子酸代谢产物、13种鞣花酸代谢产物、37种柯里拉京代谢产物,这3种成分主要发生水解、脱羧等Ⅰ相代谢,以及甲基化、硫酸化、葡萄糖醛酸化等Ⅱ相代谢。结论该方法稳定可靠,可为探究其他天然酚类的体内代谢规律提供参考。 展开更多
关键词 没食子酸 鞣花酸 柯里拉京 代谢产物 uplc-q-tof-ms/ms
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Research on the Optimal Allocation of Community Elderly Care Service Resources Based on Big Data Technology
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作者 Shuying Li 《Journal of Clinical and Nursing Research》 2026年第1期241-246,共6页
With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service... With the accelerating aging process of China’s population,the demand for community elderly care services has shown diversified and personalized characteristics.However,problems such as insufficient total care service resources,uneven distribution,and prominent supply-demand contradictions have seriously affected service quality.Big data technology,with core advantages including data collection,analysis and mining,and accurate prediction,provides a new solution for the allocation of community elderly care service resources.This paper systematically studies the application value of big data technology in the allocation of community elderly care service resources from three aspects:resource allocation efficiency,service accuracy,and management intelligence.Combined with practical needs,it proposes optimal allocation strategies such as building a big data analysis platform and accurately grasping the elderly’s care needs,striving to provide operable path references for the construction of community elderly care service systems,promoting the early realization of the elderly care service goal of“adequate support and proper care for the elderly”,and boosting the high-quality development of China’s elderly care service industry. 展开更多
关键词 Big data technology COMMUNITY Elderly care Service resources
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Tracking a High-Tech Transition--How technology is powering Guangdong’s manufacturing transformation
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作者 HU FAN 《ChinAfrica》 2026年第1期30-32,共3页
The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them w... The moment a media delegation from the Republic of the Congo arrived at the Othello Kitchenware Museum on 18 November 2025,they were greeted with a vivid show of Guangdong’s industrial strength.Standing before them was not a typical exhibition hall,but a building shaped like a gleaming stainless-steel cooking pot. 展开更多
关键词 othello kitchenware museum technology industrial strength high tech transition guangdong manufacturing transformation
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Setting the standard! The technology that shapes 40% of the world's denim finishing
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《China Textile》 2026年第1期44-44,共1页
Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision... Jeanologia celebrated its 30th anniversary reaffirming the mission it was born with in 1994,to transform the textile industry into a more sustainable,efficient,and human-centered sector.Three decades later,that vision has become a global reality.Today,more than 40 percent of all jeans produced worldwide are made using technologies developed by the Valencia-based company.What started in a small laboratory in Valencia(Spain),with a pioneering team and a visionary idea,has evolved into a global benchmark in sustainable innovation.From the beginning,Jeanologia believed in technology as a driver of change,introducing solutions that eliminated harmful practices and opened the door to a new,cleaner way of making garments. 展开更多
关键词 technological transformation environmental impact textile industry garment production DENIM jeanologia sustainability innovation
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Chinese Rural Women's Social Media Use: An Empirical Study Adopting the Technology Acceptance Model
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作者 Zhang Hui Mahadevan Krishnan 《Contemporary Social Sciences》 2026年第1期104-122,共19页
Given the growing importance of social media in digital rural development, this study systematically investigated the influence pathways of social media use among rural women in China, drawing on the Technology Accept... Given the growing importance of social media in digital rural development, this study systematically investigated the influence pathways of social media use among rural women in China, drawing on the Technology Acceptance Model(TAM). Employing quantitative research methods, the study conducted empirical tests based on 367 valid questionnaires using Partial Least Squares Structural Equation Modeling(PLS-SEM) via SmartPLS 4.0 software. Results indicate that significant associations exist between perceived ease of use, perceived usefulness, attitudes toward use, behavioral intention, and actual usage behavior. Specifically, the study finds that rural women's perceived ease of use of social media has a significant and positive influence on both perceived usefulness and attitudes toward use. Perceived usefulness further significantly promotes attitudes toward use and behavioral intention. Moreover, positive attitudes toward usage and strong behavioral intentions were effectively converted into actual social media usage behaviors. This study not only validates the applicability and explanatory power of the TAM model in understanding the digital behavior of Chinese rural women but also provides quantitative evidence for how social media enhances their “digital visibility.” These findings offer practical insights for governments and platform providers to optimize user experiences and strengthen digital skills training. Despite its limitations, including a cross-sectional design and a regional sample, this research holds significant theoretical and practical implications. 展开更多
关键词 Chinese rural women social media technology Acceptance Model(TAM) digital visibility
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Between Themis and Prometheus:a history of the ethics of science and technology
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作者 Jia-Yun Chen Zong-Zhen Wu +7 位作者 Qiang-Yu Xiang Lu Zhang Jia-Lin Diao Ya-Hui Zhang Shen-Shen Yan Kun-Lan Zuo Jian-Liang Huang Huan Liu 《History and Philosophy of Medicine》 2026年第1期49-59,共11页
Background:With the rapid development of modern emerging technologies,the ethical dilemmas and social controversies triggered by scientific and technological activities have become increasingly prominent.How to guide ... Background:With the rapid development of modern emerging technologies,the ethical dilemmas and social controversies triggered by scientific and technological activities have become increasingly prominent.How to guide technology for good and prevent and control technological risks has become an important issue of global concern.Research on science and technology ethics is dedicated to integrating ethical theories into governance practices and constructing ethical models that adapt to the development of the times.Methods:This article systematically reviews the six core approaches of scientific and technological ethics thought,including technological autonomy and political philosophy criticism,responsibility ethics and intergenerational obligations,technological intermediation and the integration of life and the world,ethical principles and normative frameworks,participatory governance and ethical practice innovation,as well as domain-specific ethical norms,thereby constructing an ethical analysis framework applicable to medical technology risks.And cross-analysis was conducted by taking medical events such as gene editing and xenotransplantation as examples.Results:Research shows that a single ethical approach has limitations in addressing complex medical ethical challenges,while the six approaches are complementary and synergistic.By criticizing technological autonomy,establishing a responsibility ethics orientation,setting the bottom line of ethical principles,promoting participatory governance,formulating domain norms,and continuously reflecting on the intermediary nature of technology,a multi-level and dynamically adaptive governance system for scientific and technological ethics can be constructed.Conclusion:The key to addressing contemporary medical ethics challenges lies in the comprehensive application of science and technology ethics theories and the integration of ethical considerations throughout the entire process of scientific and technological research and development.In the future,a governance framework that adapts to the development of new technologies should be established to promote cross-cultural and cross-disciplinary ethical dialogue and public participation,ensuring that scientific and technological innovation always serves the dignity of human life and overall well-being. 展开更多
关键词 ethics of science and technology history of science ethical governance responsible innovation medical ethics
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Teaching Reform of Chinese Medicine Processing Technology Course under the Integration of Technical and Higher Vocational Education
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作者 Lianfang LI 《Medicinal Plant》 2026年第1期72-77,82,共7页
Against the backdrop of integrated development between technical education and higher vocational education,the teaching of Chinese Medicine Processing Technology courses faces new opportunities and challenges.This pap... Against the backdrop of integrated development between technical education and higher vocational education,the teaching of Chinese Medicine Processing Technology courses faces new opportunities and challenges.This paper analyzes the existing problems in the current teaching of Chinese Medicine Processing Technology courses,discusses the necessity of reforming the teaching model under the context of integration,and proposes the construction of a"Dual-Capability Progression,Six-Dimensional Empowerment"teaching model.The aim is to enhance the teaching quality of Chinese Medicine Processing Technology courses and cultivate high-quality skilled talents in Chinese medicine processing who can meet industry demands. 展开更多
关键词 Technical education Higher vocational education Integrated development Chinese Medicine Processing technology Course Teaching reform
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Optical lateral flow immune assay technology for body fluid sensing
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作者 Chen Liu Tianqi Zhao +5 位作者 Jialing Zhou Xiaoyun Hu Dinghao Pan Jinlong Li Wei Li Zhihui Dai 《Chinese Chemical Letters》 2026年第1期106-115,共10页
Detecting biomarkers in body fluids by optical lateral flow immune assay(LFIA) technology provides rapid access to disease information for early diagnosis.LFIA is based on an antigen-antibody reaction and is rapidly b... Detecting biomarkers in body fluids by optical lateral flow immune assay(LFIA) technology provides rapid access to disease information for early diagnosis.LFIA is based on an antigen-antibody reaction and is rapidly becoming the preferred choice of physicians and patients for point-of-care testing due to its simplicity,cost-effectiveness,and rapid detection.Observing the optical signal change from the colloidal gold of the traditional LFIA strip has been widely applied for various biomarkers detection in body fluids.Despite the significant progress,rapid real-time detection of color changes in the colloidal gold by the naked eye still faces many limitations,such as large errors and the inability to quantify and accurately detect.New optical LFIA strip technology has emerged in recent years to extend its application scenarios for achieving quantitative detection such as fluorescence,afterglow,and chemiluminescence.Herein,we summarized the development of optical LFIA technology from single to hyphenated optical signals for biomarkers detection in body fluids from invasive and non-invasive sources.Moreover,the challenge and outlook of optical LFIA strip technology are highlighted to inspire the designing of next-generation diagnostic platforms. 展开更多
关键词 Optical signal Lateral flow immune assay Hyphenated optical technology Body fluid sensing Point-of-care testing
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Application of Whole Slide Imaging Technology in Clinical Pathological Diagnosis
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作者 Zhenjie Wang Jianguo Huai 《Journal of Clinical and Nursing Research》 2026年第1期21-26,共6页
Whole Slide Imaging (WSI) technology, as a revolutionary digital technology in the field of pathology, is gradually changing the traditional clinical pathological diagnosis model. By converting traditional glass patho... Whole Slide Imaging (WSI) technology, as a revolutionary digital technology in the field of pathology, is gradually changing the traditional clinical pathological diagnosis model. By converting traditional glass pathological sections into complete digital images through high-resolution scanning, it provides a new method for pathological diagnosis. Based on this, this paper studies the application of WSI technology in clinical pathological diagnosis, elaborates on its application value, analyzes the current application status, and proposes corresponding application countermeasures, aiming to provide reference for the standardized and popularized development of this technology in clinical pathological diagnosis. 展开更多
关键词 Whole slide imaging(WSI)technology Clinical pathological diagnosis Application value Current status Application countermeasures
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A review of microfluidic technology for CO_(2)sequestration in saline aquifers
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作者 Yiyan Zhong Qi Li +2 位作者 Wenbin Gao Yiping Wen Yao Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第1期810-829,共20页
In recent years,microfluidic technology has emerged as a powerful and innovative tool,attracting significant attention for its ability to provide real-time visualization of CO_(2)flow,mass transfer,and reaction proces... In recent years,microfluidic technology has emerged as a powerful and innovative tool,attracting significant attention for its ability to provide real-time visualization of CO_(2)flow,mass transfer,and reaction processes in porous media.This review examines the application of microfluidic technology in CO_(2)sequestration in saline aquifers,emphasizing the advantages of saline aquifer for geological sequestration,including safety,high storage capacity,stability,and cost-effectiveness.The materials used for microfluidic chips and the design of microchannels are systematically reviewed,offering forward-looking recommendations for chip selection and microchannel characterization in future research on CO_(2)sequestration in saline aquifer.Based on a detailed analysis of advancements in microfluidic technology,this review highlights key findings related to CO_(2)trapping mechanisms,salt precipitation,and CO_(2)-water-rock chemical interactions within saline aquifers.Although microfluidic technology shows great promise in these areas,this review identifies limitations in current studies and outlines future research directions,aiming to promote further innovation and broader application of microfluidic technology in the field of CO_(2)sequestration in saline aquifer. 展开更多
关键词 Microfluidic technology Chip materials Microchannel fabrication CO_(2)trapping mechanisms Salt precipitation CO_(2)-water-rock interaction
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基于UPLC-Q-TOF-MS/MS及网络药理学探讨活络效灵丹治疗糖尿病周围神经病变的物质基础研究 被引量:3
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作者 张喆 李玉琴 +5 位作者 施亚宁 宿树兰 范若颖 尚尔鑫 朱悦 段金廒 《药学学报》 北大核心 2025年第4期1124-1136,共13页
本实验通过超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS/MS)、网络药理学探讨活络效灵丹治疗糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)的物质基础。利用UPLC-Q-TOF-MS/MS技术结合PeakView^(TM)1.2软件、Molec... 本实验通过超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS/MS)、网络药理学探讨活络效灵丹治疗糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)的物质基础。利用UPLC-Q-TOF-MS/MS技术结合PeakView^(TM)1.2软件、Molecule Profiler^(TM)软件分析活络效灵丹的体外成分及正常大鼠与高脂饲料喂养联合小剂量链脲佐菌素注射所致DPN大鼠的入血成分,通过Swiss Target Prediction、GeneCards等数据库筛选成分对应靶点及疾病靶点,利用Venny 2.1平台获得交集靶点,导入STRING平台及Cytoscape 3.10.1软件构建蛋白互作网络及“成分-靶点”网络图分析其核心成分与核心靶点,对核心靶点进行GO和KEGG富集分析。实验获得南京中医药大学实验动物伦理委员会批准(批准号:202310A023)。结果共鉴定出活络效灵丹提取物83个化学成分,包括丹参酮ⅡA等52个萜类、Z-藁本内酯等11个苯酞类、阿魏酸等15个有机酸类及4个香豆素类、1个酚类成分。在正常大鼠血浆中鉴定出15个原型成分与29个代谢产物,在DPN大鼠血浆中鉴定出17个原型成分与32个代谢产物。网络药理学结果显示,3-乙酰基-11-酮基-β-乳香酸、11-酮基-β-乳香酸等5个核心成分可能通过TNF、IL6等27个核心靶点,调节AGE-RAGE、PI3K/Akt等信号通路发挥治疗DPN的作用。本研究通过UPLC-Q-TOF-MS/MS技术分析了活络效灵丹的体内外化学成分及主要化合物的裂解规律,结合网络药理学分析其治疗DPN的核心成分,为阐明活络效灵丹治疗DPN的功效物质基础及作用机制研究提供科学依据。 展开更多
关键词 活络效灵丹 uplc-q-tof-ms/ms 网络药理学 糖尿病周围神经病变 化学成分 入血成分
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基于UPLC-Q-TOF-MS/MS技术分析龙脷叶醇提物化学成分与咳嗽大鼠体内入血成分和代谢原型成分 被引量:3
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作者 丘琴 覃芳芳 +4 位作者 韦红杏 叶梅芳 甄丹丹 甄汉深 宋铭 《药物评价研究》 北大核心 2025年第1期157-166,共10页
目的分析鉴定龙脷叶Sauropus spatulifolius Beille 75%乙醇提取物化学成分及咳嗽大鼠体内入血成分和代谢原型成分。方法通过UPLC-Q-TOF-MS/MS技术分析龙脷叶75%乙醇提取物化学成分;雄性SD大鼠36只,随机分为6组:对照组、模型组、枸橼酸... 目的分析鉴定龙脷叶Sauropus spatulifolius Beille 75%乙醇提取物化学成分及咳嗽大鼠体内入血成分和代谢原型成分。方法通过UPLC-Q-TOF-MS/MS技术分析龙脷叶75%乙醇提取物化学成分;雄性SD大鼠36只,随机分为6组:对照组、模型组、枸橼酸喷托维林片(阳性药,10.521 mg·kg^(-1))组和龙脷叶75%乙醇提取物高、中、低剂量(26.31、13.15、6.58 mg·kg^(-1))组,每天1次,连续给药7 d,对照组和模型组均ig等量纯净水。在第6天给药后1 h和末次给药后1 h,应用辣椒素溶液建立大鼠咳嗽模型,根据药效实验结果,取对照组和提取物中剂量组的血清、对照组和高剂量组的粪便和尿液样本,利用UPLC-Q-TOF-MS/MS技术鉴定入血成分及粪便和尿液中的代谢原型成分。结果鉴定出龙脷叶75%乙醇提取物化学成分40个,以有机酸类为主,从血清样本分析出大黄素、芹菜素等19个入血原型成分,从粪便样本中分析出咖啡酸、异落叶松脂素等17个原型成分,尿液样本中发现咖啡酸、东莨菪亭等22个原型成分。结论确定了龙脷叶75%乙醇提取物中的化学成分、入血原型成分以及在粪便和尿液中的代谢原型成分,推测黄酮类和有机酸类成分可能是龙脷叶发挥止咳作用的药效物质。 展开更多
关键词 龙脷叶 醇提取物 uplc-q-tof-ms/ms 化学成分 咳嗽 入血成分 代谢原型成分 有机酸类 黄酮类
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UPLC-Q-TOF-MS/MS法分析鹿芪益肾颗粒化学成分 被引量:1
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作者 时扣荣 徐婕 +8 位作者 李婷 刘娟 辛蓓玮 翟巧利 侯晓丽 朱秋珍 李媛 黎翠萍 范伟 《中成药》 北大核心 2025年第4期1195-1204,共10页
目的建立UPLC-Q-TOF-MS/MS法分析鹿芪益肾颗粒化学成分。方法分析采用Agilent Eclipse Plus C_(18)UPLC色谱柱(2.1 mm×150 mm,1.8μm);流动相乙腈-水(含0.1%甲酸),梯度洗脱;体积流量0.2 mL/min;柱温35℃;电喷雾离子源;正负离子扫... 目的建立UPLC-Q-TOF-MS/MS法分析鹿芪益肾颗粒化学成分。方法分析采用Agilent Eclipse Plus C_(18)UPLC色谱柱(2.1 mm×150 mm,1.8μm);流动相乙腈-水(含0.1%甲酸),梯度洗脱;体积流量0.2 mL/min;柱温35℃;电喷雾离子源;正负离子扫描。根据保留时间、准分子离子峰、二级碎片离子,结合对照品和数据库鉴定化学成分。结果共鉴定出160种成分,包括45种黄酮类、27种有机酸及酚酸类、34种生物碱类、15种萜类、11种氨基酸类、28种其他类。结论该方法稳定可靠,明确了鹿芪益肾颗粒物质基础,可为其质量控制提供科学依据。 展开更多
关键词 鹿芪益肾颗粒 化学成分 uplc-q-tof-ms/ms
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基于UPLC-Q-TOF-MS/MS结合网络药理学探讨银翘散抗流感病毒的药效物质基础及潜在作用机制
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作者 张晓艳 黄懿 +3 位作者 陈林洋 谢苗 赵鹏 李杰 《药物评价研究》 北大核心 2025年第12期3595-3612,共18页
目的基于UPLC-Q-TOF-MS/MS结合网络药理学探讨银翘散抗流感病毒的药效物质基础及潜在作用机制。方法基于性味理论,研究将银翘散拆方为辛味组(金银花、桔梗、薄荷、牛蒡子、荆芥、生甘草)与苦味组(连翘、淡豆豉、淡竹叶、生甘草),并通过... 目的基于UPLC-Q-TOF-MS/MS结合网络药理学探讨银翘散抗流感病毒的药效物质基础及潜在作用机制。方法基于性味理论,研究将银翘散拆方为辛味组(金银花、桔梗、薄荷、牛蒡子、荆芥、生甘草)与苦味组(连翘、淡豆豉、淡竹叶、生甘草),并通过观察银翘散及其拆方对流感风热证小鼠的保护作用,结合血清药物化学及网络药理学方法评价银翘散及其拆方抗流感的有效活性成分,探究其抗流感病毒的“味-效”物质基础,预测其可能的作用机制。结果银翘散及其拆方对流感风热证小鼠均具有一定的保护作用。其中,在维持小鼠体质量及降低肺指数方面,辛味组较苦味组改善效果更优;苦味组在维持大便湿质量、降低中性粒细胞百分比、升高淋巴细胞比例方面,效果优于辛味组。总体来看,银翘散在各方面改善效果最为显著。基于UPLC-Q-TOF-MS/MS技术鉴定出银翘散中725个化学成分、73个入血成分,辛味组中617个化学成分、53个入血成分,苦味组中595个化学成分、47个入血成分。网络药理学显示,银翘散及其拆方中35个共同靶点(TP53、STAT3等)通过调控病毒生命周期(介导病毒内化、参与病毒复制等)与宿主防御(免疫反应、炎症反应等),构成抗流感病毒的共性作用基础。辛味组对Th17细胞分化与人体免疫缺陷病毒1感染通路的特异性激活,苦味组对病灶黏附、细胞凋亡与TNF信号通路的显著干预,可能是其发挥“辛味”与“苦味”功效的分子机制。结论该研究揭示银翘散及其拆方的药效学物质基础,预测其潜在的抗病毒作用可能与炎症反应、免疫反应等相关信号通路有关,为银翘散的临床应用提供理论依据。 展开更多
关键词 银翘散 物质基础 uplc-q-tof-ms 辛味 网络药理学
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基于UPLC-Q-TOF-MS/MS网络药理学的九制黄精与生黄精对脂多糖诱导RAW 264.7细胞炎症反应的抑制作用差异研究
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作者 严志宏 葛浩宇 +5 位作者 罗珍 许金娣 尹雪琴 彭希帆 蔡瑛 陈丽华 《药物评价研究》 北大核心 2025年第12期3551-3569,共19页
目的采用UPLC-Q-TOF-MS/MS技术结合网络药理学及体外细胞实验,探讨九制黄精与生黄精对脂多糖(LPS)诱导的RAW 264.7巨噬细胞炎症反应的抑制作用差异。方法通过UPLC-Q-TOF-MS/MS技术结合文献检索,对比分析九制黄精与生黄精的化学成分差异... 目的采用UPLC-Q-TOF-MS/MS技术结合网络药理学及体外细胞实验,探讨九制黄精与生黄精对脂多糖(LPS)诱导的RAW 264.7巨噬细胞炎症反应的抑制作用差异。方法通过UPLC-Q-TOF-MS/MS技术结合文献检索,对比分析九制黄精与生黄精的化学成分差异;基于已鉴定成分筛选黄精抗炎的关键活性成分与核心作用靶点,构建“中药-成分-靶点-疾病”调控网络,并通过分子对接验证核心成分与靶点的结合能力;将不同质量浓度(1、10、50、100、200、400、800μg·mL^(-1))的九制黄精、生黄精水提物(NPA、RPA)分别作用于RAW 264.7细胞24 h,采用CCK-8法检测细胞活力;使用1μg·mL^(-1)LPS诱导RAW 264.7细胞建立炎症模型,实验设对照组、模型组(1μg·mL^(-1)LPS)、RPA组(100、200、400μg·mL^(-1))及NPA组(100、200、400μg·mL^(-1)),采用ELISA法检测各组细胞上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的分泌水平,比较二者抗炎作用差异。结果共鉴定出114种化学成分,其中82种为九制黄精与生黄精共有,21种为九制黄精特有成分,11种为生黄精特有成分;网络药理学分析筛选获得873个黄精抗炎相关交集靶点,确定延龄草苷、N-阿魏酰真胺、麝香草酚、丁香脂素、静特诺皂苷元为核心活性成分,转导和转录激活因子(STAT3)、蛋白激酶B(AKT1)、缺氧诱导因子1亚基α(HIF1A)、肿瘤坏死因子(TNF)、白细胞介素-6(IL-6)和B细胞淋巴瘤/白血病-2基因(BCL2)为核心靶点,基因本体(GO)功能注释与京都基因与基因组百科全书(KEGG)通路富集分析显示,磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)、高级糖基化终末产物(AGE)-受体(RAGE)信号通路是黄精发挥抗炎作用的潜在关键通路;体外细胞毒性实验表明,各质量浓度的NPA及400μg·mL^(-1)以下的RPA对RAW 264.7细胞存活率均无显著影响(P>0.05),相同质量浓度下NPA组细胞存活率略高于RPA组,但差异无统计学意义(P>0.05);抗炎活性评价显示,与对照组相比,模型组细胞上清液中TNF-α、IL-6水平显著升高(P<0.01);与模型组相比,NPA、RPA各质量浓度组均能显著抑制LPS诱导的TNF-α、IL-6分泌(P<0.01);在400μg·mL^(-1)浓度下,RPA组TNF-α水平低于NPA组,而各质量浓度下RPA组IL-6分泌量均高于NPA组。结论九制黄精的化学成分较生黄精更丰富,二者均能有效缓解LPS诱导的RAW 264.7细胞炎症反应,但在调控炎症因子分泌方面存在差异;九制黄精的抗炎作用可能与其特有成分及调控PI3K-Akt、AGE-RAGE等信号通路相关。 展开更多
关键词 九制黄精 炎症 uplc-q-tof-ms/ms 成分分析 网络药理学 静特诺皂苷元
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基于UPLC-Q-TOF-MS和UPLC-QqQ-MS/MS的山茱萸不同炮制品化学成分定性与定量分析 被引量:4
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作者 张立强 单国顺 +5 位作者 洪艺丹 刘思翰 徐国伟 高慧 王巍 鞠成国 《中国中药杂志》 北大核心 2025年第8期2145-2158,共14页
建立山茱萸不同炮制品化学成分定性与定量分析方法,系统地表征与鉴定其化学成分并测定主要差异成分的含量。采用UPLC-Q-TOF-MS技术对山茱萸不同炮制品化学成分进行数据采集,通过自建数据库、文献及对照品等信息进行分析鉴定,共得到93个... 建立山茱萸不同炮制品化学成分定性与定量分析方法,系统地表征与鉴定其化学成分并测定主要差异成分的含量。采用UPLC-Q-TOF-MS技术对山茱萸不同炮制品化学成分进行数据采集,通过自建数据库、文献及对照品等信息进行分析鉴定,共得到93个成分,其中环烯醚萜类19个、黄酮类15个、有机酸类16个、三萜类8个、鞣质类8个、氨基酸类4个、多糖类2个、烯烃类5个、其他类16个。同时结合多元统计学分析,以VIP>1.0、FC<0.5或>2.0且P<0.05为条件筛选山茱萸不同炮制品之间的差异成分。主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)结果表明山茱萸不同炮制品之间化学成分存在差异,共筛选得到酒石酸、莫诺苷、芦丁等21个差异成分。在此基础上,采用UPLC-QqQ-MS/MS技术测定没食子酸、莫诺苷、熊果酸、马钱苷、獐牙菜苷、芦丁、5-羟甲基糠醛、山茱萸新苷Ⅰ、槲皮素、齐墩果酸等10种主要差异成分的含量。上述10种成分在测定浓度范围内线性关系良好,精密度、稳定性、重复性及加样回收率均符合要求。与山萸肉比,酒萸肉和酒蜜萸肉环烯醚萜苷类含量降低,没食子酸、芦丁、槲皮素、5-羟甲基糠醛、熊果酸和齐墩果酸含量升高;与酒萸肉比,酒蜜萸肉除没食子酸含量略有降低外,其余成分均有不同程度的升高。综上,该研究明确了不同炮制方法对山茱萸化学成分的影响,为后续揭示山茱萸炮制的科学内涵、整体质量评价以及临床合理应用提供理论依据。 展开更多
关键词 山茱萸 炮制 化学成分 uplc-q-tof-ms UPLC-QqQ-MS/MS
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基于UPLC-Q-TOF-MS的苦参药材和药渣的化学成分研究 被引量:4
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作者 刘倩文 朱荣清 +7 位作者 胡倩南 李翔 杨光 邱子栋 詹志来 南铁贵 陈美兰 康利平 《中国中药杂志》 北大核心 2025年第3期708-718,共11页
苦参为我国常用中药之一,其在加工生产过程中产生的大量药渣作为垃圾废弃,既浪费资源又污染环境。因此,阐明苦参药渣的化学成分组成及其与苦参的差异对苦参药渣的综合利用具有重要意义。该研究基于超高效液相色谱-串联四极杆飞行时间质... 苦参为我国常用中药之一,其在加工生产过程中产生的大量药渣作为垃圾废弃,既浪费资源又污染环境。因此,阐明苦参药渣的化学成分组成及其与苦参的差异对苦参药渣的综合利用具有重要意义。该研究基于超高效液相色谱-串联四极杆飞行时间质谱(UPLC-Q-TOF-MS)技术并结合多元统计分析方法,对苦参和苦参药渣的整体成分进行了良好表征、鉴定及差异分析。首先,根据化合物的精确相对分子质量、碎片离子及其丰度等信息结合自建化合物库快速鉴定了苦参药材中61个化合物,其中苦参药渣中有41个,主要为生物碱类和黄酮类成分。其次,通过主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)筛选出苦参药材和药渣之间的15个主要差异化合物,包括在药渣中含量明显降低的氧化苦参碱和氧化槐果碱等极性较强的生物碱类化合物,以及在药渣中含量更高的苦参酮和次苦参素等极性较弱的黄酮类化合物。综上,该文明确了苦参及其药渣中化合物组成、结构和含量的整体情况及差异,提示苦参药渣可作为其中高活性成分的提取原料,在美妆日护等方面具有较好的开发应用潜力,为后续苦参及其药渣的综合利用提供了科学依据。 展开更多
关键词 苦参 药渣 uplc-q-tof-ms 化学成分 表征鉴定
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基于UPLC-Q-TOF-MS和网络药理学探讨菟丝子-枸杞子药对治疗早发性卵巢功能不全的成分及机制 被引量:9
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作者 宗政 胡扬 +7 位作者 徐蓓蕾 辛国松 张文君 陈鹰翔 刘树森 孙向明 宋辉 李文兰 《中成药》 北大核心 2025年第2期649-658,共10页
目的探讨菟丝子-枸杞子药对治疗早发性卵巢功能不全的成分及机制。方法采用UPLC-Q-TOF-MS法进行分析,结合质谱信息、对照品比对、参考文献以及UNIFI软件对化合物进行指认。采用网络药理学构建“成分-靶点”网络并富集相关通路,再对关键... 目的探讨菟丝子-枸杞子药对治疗早发性卵巢功能不全的成分及机制。方法采用UPLC-Q-TOF-MS法进行分析,结合质谱信息、对照品比对、参考文献以及UNIFI软件对化合物进行指认。采用网络药理学构建“成分-靶点”网络并富集相关通路,再对关键成分与核心靶点进行分子对接。结果共鉴定出110种成分,包括26种黄酮类、23种生物碱类、15种苯丙素类、31种有机酸类、15种其他类,其中菟丝子和枸杞子共有成分32种,菟丝子独有38种,枸杞子独有40种。成分与疾病交集靶点有629个,核心靶点有10个;药对主要参与丝氨酸/苏氨酸/酪氨酸蛋白激酶活性、膜筏、多肽反应等过程,通过PI3K-Akt信号通路、神经活性配体-受体相互作用、MAPK信号通路等治疗早发性卵巢功能不全;共有4个关键靶点和5种关键活性成分,药对关键成分与核心靶点展现出较强的结合能力。结论本研究通过UPLC-Q-TOF-MS结合网络药理学对菟丝子-枸杞子药对化学成分进行解析,揭示其可能通过多成分、多靶点、多途径来发挥治疗早发性卵巢功能不全的作用。 展开更多
关键词 菟丝子-枸杞子药对 早发性卵巢功能不全 化学成分 作用机制 uplc-q-tof-ms 网络药理学
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基于UPLC-Q-TOF-MS/MS法、HPLC指纹图谱、含量测定评价覆盆子质量 被引量:3
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作者 李书晚 廖扬振 +4 位作者 王龙飞 赵雅琴 范甲赫 魏渊 欧阳臻 《中成药》 北大核心 2025年第4期1077-1084,共8页
目的 评价覆盆子质量。方法 UPLC-Q-TOF-MS/MS法进行成分鉴定,建立HPLC指纹图谱,聚类分析、主成分分析、正交偏最小二乘判别分析进行化学模式识别,测定绿原酸、阿魏酸、鞣花酸、异槲皮苷、山柰酚-3-O-芸香糖苷、紫云英苷、椴树苷、槲皮... 目的 评价覆盆子质量。方法 UPLC-Q-TOF-MS/MS法进行成分鉴定,建立HPLC指纹图谱,聚类分析、主成分分析、正交偏最小二乘判别分析进行化学模式识别,测定绿原酸、阿魏酸、鞣花酸、异槲皮苷、山柰酚-3-O-芸香糖苷、紫云英苷、椴树苷、槲皮素、山柰酚的含量。结果 共鉴定出34种成分。31批药材指纹图谱中有19个共有峰,相似度均大于0.8。野生品与栽培品,以及不同产地药材可区分开;4个主成分累积方差贡献率为84.142%;筛选出8个差异性成分,其中2个为鞣花酸和紫云英苷。野生品中鞣花酸、紫云英苷含量高于栽培品中(P<0.05,P<0.01)。结论 UPLC-Q-TOF-MS/MS法、HPLC指纹图谱结合含量测定可用于覆盆子质量控制。 展开更多
关键词 覆盆子 质量评价 uplc-q-tof-ms/ms HPLC指纹图谱 含量测定 化学模式识别
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