A UPLC/Q-TOF-MS/MS method was developed for identifying the constituents in the Chinese herb Semen Descurainiae. A total of 14 compounds were identified or tentatively characterized based on their mass spectra, includ...A UPLC/Q-TOF-MS/MS method was developed for identifying the constituents in the Chinese herb Semen Descurainiae. A total of 14 compounds were identified or tentatively characterized based on their mass spectra, including 9 flavonoids, 4 fatty acids and 1 cardiac glycoside. Among them, quercetin-3-O-β-D-[6-O-sinnapoyl-2-O-β-D-glucopyranosyl]- glucopyranoside was identified in Semen Descurainiae for the first time.展开更多
目的 分析金雀花乙醇提取物的化学成分。方法 采用超高效液相色谱-四极杆-飞行时间串联质谱(Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry, UPLC-Q-TOF-MS/MS)技术,使用A...目的 分析金雀花乙醇提取物的化学成分。方法 采用超高效液相色谱-四极杆-飞行时间串联质谱(Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry, UPLC-Q-TOF-MS/MS)技术,使用ACQUITY UPLC BEH C18色谱柱,流动相为0.1%甲酸水溶液和乙腈,线性梯度洗脱;采用电喷雾离子源在m/z50~1 500范围内采集数据;采用UNIFI V 1.9.2软件对数据进行预处理、峰检测、分子式推测,通过高分辨质谱数据结合数据库、文献和对照品进行对比,分析金雀花乙醇提取物中的化学成分。结果 共分析出47种化学成分,包括27种黄酮类、13种皂苷类、7种其他类成分,其中包括山奈酚-3-O-芸香糖苷、牡荆素、金丝桃苷、山奈苷、水仙苷、芹菜素、槲皮素、金合欢素等成分。结论 本研究分析了金雀花乙醇提取物中的化学成分,为后续金雀花的开发与利用提供了理论基础。展开更多
基金National Basic Research Program of China(973 Program:Grant No.2012CB518606)National Science Fund for Distinguished Young Scholars(Grant No.81102768)
文摘A UPLC/Q-TOF-MS/MS method was developed for identifying the constituents in the Chinese herb Semen Descurainiae. A total of 14 compounds were identified or tentatively characterized based on their mass spectra, including 9 flavonoids, 4 fatty acids and 1 cardiac glycoside. Among them, quercetin-3-O-β-D-[6-O-sinnapoyl-2-O-β-D-glucopyranosyl]- glucopyranoside was identified in Semen Descurainiae for the first time.
