One hundred and forty-four F2 individuals from a cross combination between 1047(susceptible)and A21(resistant)were analyzed for the presence of random amplified polymorphic DNA(RAPD)markers linked to Turnip mosaic vir...One hundred and forty-four F2 individuals from a cross combination between 1047(susceptible)and A21(resistant)were analyzed for the presence of random amplified polymorphic DNA(RAPD)markers linked to Turnip mosaic virus(TuMV)resistant gene in cabbage by using bulked segregant analysis(BSA).Two polymorphic markers were screened out of 200 random primers.These two RAPD fragments were linked to the resistant gene at 7.7 cM and 8.38 cM respectively and converted to SCAR markers successfully.展开更多
文摘One hundred and forty-four F2 individuals from a cross combination between 1047(susceptible)and A21(resistant)were analyzed for the presence of random amplified polymorphic DNA(RAPD)markers linked to Turnip mosaic virus(TuMV)resistant gene in cabbage by using bulked segregant analysis(BSA).Two polymorphic markers were screened out of 200 random primers.These two RAPD fragments were linked to the resistant gene at 7.7 cM and 8.38 cM respectively and converted to SCAR markers successfully.