In order to solve the problem of intraperitoneal haemorrhage and bile leakage caused by the iatrogenic trauma, sixty-four sequence of puncture were performed on twenty canine livers and thirty-two sequences selected b...In order to solve the problem of intraperitoneal haemorrhage and bile leakage caused by the iatrogenic trauma, sixty-four sequence of puncture were performed on twenty canine livers and thirty-two sequences selected by chance were perfused with thrombin solution. The results revealed that the bleeding time was shortened and amounts lessened significantly after application of thrombin compared with counterparts of control (P< 0. 001,t test).There was no significant difference between the levels of plasma fibringgeu prc-and Post-thrombin's using (P > 0.4,t test).The thrombin clotting time of patients with obstructive jaundice was similar to that of the group of control(P> 0. 05, t test).We conclude that thrombin is characterized by the ability of rapid,effective and safe haemostasis to the traumatic channel made by the liver puncture,and it's adaptive to most of the patients with hepatobiliary disease,and worth using and developing in clinical practice.展开更多
The purpose of the present study was to determine protectivie effects of basic fibroblast growth factor (bFGF) on cochlear neurons and hair cells in vitro and in vivo. In experiment I, cultured spiral ganglion neurons...The purpose of the present study was to determine protectivie effects of basic fibroblast growth factor (bFGF) on cochlear neurons and hair cells in vitro and in vivo. In experiment I, cultured spiral ganglion neurons (SGNs) prepared from P3 mice were exposed to 20mM glutamate for 2 hours before the culture medium was replaced with fresh medium containing 0, 25, 50, and 100 ng/ml bFGF, respectively. Fourteen days later, all cultures were fixed with 4% paraformaldehyde, and stained with 1% toluidine blue. The number of surviving SGNs were counted and the length of SGNs neurites were measured. Exposure to 20 mM glutamate for 24 hours resulted in an inhibition on neurite outgrowth of SGNs and elevated cell death. Treatment of the cultures with bFGF led to promotion of neurite outgrowth and elevated number of surviving SGNs. Effects of bFGF were dose dependent with the highest potency at 100 ng/ml. In experiment Ⅱ, in vivo studies were carried out with guinea pigs in which bFGF or artificial perilymph was perfused into the cochlea to assess possible protective effects of bFGF on cochlear hair cells and compound action potentials(CAP). The CAPs were measured before, immediatly and 48 hours after exposure to noise. Significant differences in CAP were observed (p＜0. 05 ) among the bFGF perfused group, control group(t =3. 896 ) and artificial perilymph perfused group (t =2. 520) at 48 hours after noise exposure, Cochleae were removed and hair cell Loss was analyzed in surface preparations prepared from all experimental animals. Acoustic trauma caused loss of 651 and 687 inner hair cells in the control and artificial perilymph perfused group, respectively. In sharp contrast, only 31 inner hair cells were lost in the bFGF perfused ears. Similarly, more outer hair cells died in the control and perilymph perfuesed group (41830 and 41968, respectively) than in the group treated with bFGF (34258). Our results demonstrate that bFGF protected SGNs against glutmate neurotoxicity in vitro. In addition, treatment with bFGF also protected hair cells from acoustic trauma.展开更多
To investigate the dynamics of plasma cAMP/cGMP in patients during cardiac surgery, and its relationship to traumatic stress. Methods: Sixteen patients, aged 19.31 years± 10.4 years , who underwent an open heart ...To investigate the dynamics of plasma cAMP/cGMP in patients during cardiac surgery, and its relationship to traumatic stress. Methods: Sixteen patients, aged 19.31 years± 10.4 years , who underwent an open heart operation with cardiopulmonary bypass (CPB) and hy pother mia were served as subjects. The arterial plasma concentrations of cyclic adenos ine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were measured by radioimmunoassay 2 hours before operation, after heparinization, 20 minutes following CPB, at the end of the operation, and 24 and 72 hours postoperatively, respectiv ely. The patients preoperative blood samples were heparinized and the venous b lood samples of 30 healthy blood donors were taken to measure the levels of cAMP and cGMP as heparin and normal controls separately. Results: There were no statistical difference among the heparin control, preoperative level and normal control. The peak values of cAMP and cGM P occurred during CPB and plasma cAMP levels changed synchronously with intensit ies of operative stimulus to human body. However cGMP level was mainly related t o the operative stimulus to the heart and CPB. The cAMP value was positivel y correlated with the cGMP value (r= 0.6313 , P< 0.001 ).Conclusions: Dynamic variation of plasma cyclic ribonucleotide can be considered as a reference parameter for intensity of traumatic stress.展开更多
文摘In order to solve the problem of intraperitoneal haemorrhage and bile leakage caused by the iatrogenic trauma, sixty-four sequence of puncture were performed on twenty canine livers and thirty-two sequences selected by chance were perfused with thrombin solution. The results revealed that the bleeding time was shortened and amounts lessened significantly after application of thrombin compared with counterparts of control (P< 0. 001,t test).There was no significant difference between the levels of plasma fibringgeu prc-and Post-thrombin's using (P > 0.4,t test).The thrombin clotting time of patients with obstructive jaundice was similar to that of the group of control(P> 0. 05, t test).We conclude that thrombin is characterized by the ability of rapid,effective and safe haemostasis to the traumatic channel made by the liver puncture,and it's adaptive to most of the patients with hepatobiliary disease,and worth using and developing in clinical practice.
文摘The purpose of the present study was to determine protectivie effects of basic fibroblast growth factor (bFGF) on cochlear neurons and hair cells in vitro and in vivo. In experiment I, cultured spiral ganglion neurons (SGNs) prepared from P3 mice were exposed to 20mM glutamate for 2 hours before the culture medium was replaced with fresh medium containing 0, 25, 50, and 100 ng/ml bFGF, respectively. Fourteen days later, all cultures were fixed with 4% paraformaldehyde, and stained with 1% toluidine blue. The number of surviving SGNs were counted and the length of SGNs neurites were measured. Exposure to 20 mM glutamate for 24 hours resulted in an inhibition on neurite outgrowth of SGNs and elevated cell death. Treatment of the cultures with bFGF led to promotion of neurite outgrowth and elevated number of surviving SGNs. Effects of bFGF were dose dependent with the highest potency at 100 ng/ml. In experiment Ⅱ, in vivo studies were carried out with guinea pigs in which bFGF or artificial perilymph was perfused into the cochlea to assess possible protective effects of bFGF on cochlear hair cells and compound action potentials(CAP). The CAPs were measured before, immediatly and 48 hours after exposure to noise. Significant differences in CAP were observed (p＜0. 05 ) among the bFGF perfused group, control group(t =3. 896 ) and artificial perilymph perfused group (t =2. 520) at 48 hours after noise exposure, Cochleae were removed and hair cell Loss was analyzed in surface preparations prepared from all experimental animals. Acoustic trauma caused loss of 651 and 687 inner hair cells in the control and artificial perilymph perfused group, respectively. In sharp contrast, only 31 inner hair cells were lost in the bFGF perfused ears. Similarly, more outer hair cells died in the control and perilymph perfuesed group (41830 and 41968, respectively) than in the group treated with bFGF (34258). Our results demonstrate that bFGF protected SGNs against glutmate neurotoxicity in vitro. In addition, treatment with bFGF also protected hair cells from acoustic trauma.
文摘To investigate the dynamics of plasma cAMP/cGMP in patients during cardiac surgery, and its relationship to traumatic stress. Methods: Sixteen patients, aged 19.31 years± 10.4 years , who underwent an open heart operation with cardiopulmonary bypass (CPB) and hy pother mia were served as subjects. The arterial plasma concentrations of cyclic adenos ine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were measured by radioimmunoassay 2 hours before operation, after heparinization, 20 minutes following CPB, at the end of the operation, and 24 and 72 hours postoperatively, respectiv ely. The patients preoperative blood samples were heparinized and the venous b lood samples of 30 healthy blood donors were taken to measure the levels of cAMP and cGMP as heparin and normal controls separately. Results: There were no statistical difference among the heparin control, preoperative level and normal control. The peak values of cAMP and cGM P occurred during CPB and plasma cAMP levels changed synchronously with intensit ies of operative stimulus to human body. However cGMP level was mainly related t o the operative stimulus to the heart and CPB. The cAMP value was positivel y correlated with the cGMP value (r= 0.6313 , P< 0.001 ).Conclusions: Dynamic variation of plasma cyclic ribonucleotide can be considered as a reference parameter for intensity of traumatic stress.
