The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches...The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches were obtained as follow : the cell suspending mixture obtained the highest enzyme activity(20.4U/mL),after being treated with by Triton X-100 and β-ME(5%,v/v) and ultrasonic.The enzyme activity obtained by this method was obviously higher than by single treating method,e.g.ultrasonic(7.6U/mL) or Triton X-100 and β-ME(15.2U/mL).展开更多
A study was undertaken to collect and identify saprobic fungi associated with Musa spp.(banana)from Taiwan(China),and Thailand.Samples were collected during the dry season and their morpho-molecular relationships were...A study was undertaken to collect and identify saprobic fungi associated with Musa spp.(banana)from Taiwan(China),and Thailand.Samples were collected during the dry season and their morpho-molecular relationships were investigated.Five brown pleosporalean hyphomycetous taxa in Periconiaceae and Torulaceae viz.Periconia cortaderiae,P.delonicis,Torula chromolaenae,T.fici,and T.masonii were identified for the first time from Musa spp.(Musaceae).Phylogenetic analyses of a combined SSU,LSU,ITS,RPB2 and TEF DNA sequence dataset further justified the taxonomic placements of these five taxa in the above mentioned families.Periconia delonicis is reported for the first time on a monocotyledonous host and T.masonii is the first geographical record from Taiwan(China).展开更多
文摘The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches were obtained as follow : the cell suspending mixture obtained the highest enzyme activity(20.4U/mL),after being treated with by Triton X-100 and β-ME(5%,v/v) and ultrasonic.The enzyme activity obtained by this method was obviously higher than by single treating method,e.g.ultrasonic(7.6U/mL) or Triton X-100 and β-ME(15.2U/mL).
基金supported by Key Research Project“Agroforestry Systems for restoration and bio-industry technology development(grant no.2017YFC0505101)”.We also thank Biology Experimental Center,Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences for providing the facilities of molecular laboratory.Binu C.Samarakoon is grateful to Danushka Tennakoon for collecting the specimens from Taiwan(China),Dr.Dhanushka N.Wanasinghe and Junfu Li for the valuable comments and suggestions on the morphological studies of Periconia and Torula.Rungtiwa Phookamsak thanks CAS President’s International Fellowship Initiative(PIFI)for young staff(grant no.Y9215811Q1)the National Science Foundation of China(NSFC)project code 31850410489(grant no.Y81I982211)+3 种基金Chiang Mai University for financial support.Samantha C.Karunarathna thanks CAS President’s International Fellowship Initiative(PIFI)young staff under the grant number:2020FYC0002the National Science Foundation of China(NSFC)for funding this work under the project code 31851110759Jianchu Xu thanks Key Research Program of Frontier Sciences of the Chinese Academy of Sciences(grant no.QYZDY-SSW-SMC014)the Strategic Priority Research Program of Chinese Academy of Sciences for supporting this research.
文摘A study was undertaken to collect and identify saprobic fungi associated with Musa spp.(banana)from Taiwan(China),and Thailand.Samples were collected during the dry season and their morpho-molecular relationships were investigated.Five brown pleosporalean hyphomycetous taxa in Periconiaceae and Torulaceae viz.Periconia cortaderiae,P.delonicis,Torula chromolaenae,T.fici,and T.masonii were identified for the first time from Musa spp.(Musaceae).Phylogenetic analyses of a combined SSU,LSU,ITS,RPB2 and TEF DNA sequence dataset further justified the taxonomic placements of these five taxa in the above mentioned families.Periconia delonicis is reported for the first time on a monocotyledonous host and T.masonii is the first geographical record from Taiwan(China).
