The present study aimed at isolating,purifying,characterizing the structure and estimating the anti-inflammatory activity of a polysaccharide named THSA which was obtained from Thamnolia subuliformis(Ehrh.) W.Culb(T.s...The present study aimed at isolating,purifying,characterizing the structure and estimating the anti-inflammatory activity of a polysaccharide named THSA which was obtained from Thamnolia subuliformis(Ehrh.) W.Culb(T.subuliformis).A newheteroglycan THSA was gained by hot water extraction and further continuously purification through Sephadex G-75 and Superdex 200 column chromatography.The structural characteristics of THSA were primarily investigated by molecular mass estimation,monosaccharide composition analysis and Fourier transforminfrared spectroscopy(FT-IR).The anti-inflammatory effects of THSA was also estimated in lipopolysaccharide(LPS)-stimulated mouse macrophages cell line RAW264.7 through the method of reverse transcription polymerase chain reaction(RT-PCR) and quantitative real-time PCR.The results indicate that THSA is composed of Glc,Gal,Man in the ratio 1:2.1:6.16,with average molecular weight of about 12.4 k Da,and significantly inhibits the mRNA expression of tumor necrosis factor alpha(TNF-α),IL-6 at100 or 1 000 μg/m L and increases the anti-inflammatory cytokine level of IL-10 at 100 μg/m L.It indicates that polysaecharide THSA has strong anti-inflammatory activity.展开更多
A new phenolic compound, thamnolin (1), was isolated from the extract of Thamnolia vermicularis. Its structure was determined as 6-tricosyl-2,4-dimethoxy-phenol by spectral methods.
探讨雪茶提取物[Extractive of Thamnolia vermicularis(ACH)Asahina]的抗疲劳作用效果。方法:取小鼠,随机分组,实验设高、低剂量组分、空白组、阳性药物组。雪茶提取物应用灌胃给药方法,通过各组小鼠负重游泳实验,对雪茶的抗疲劳作用...探讨雪茶提取物[Extractive of Thamnolia vermicularis(ACH)Asahina]的抗疲劳作用效果。方法:取小鼠,随机分组,实验设高、低剂量组分、空白组、阳性药物组。雪茶提取物应用灌胃给药方法,通过各组小鼠负重游泳实验,对雪茶的抗疲劳作用进行物理性研究;通过测试小鼠游泳后和静置一定时间后血液中血糖变化,血乳酸变化,肝糖原含量及血尿素含量,对雪茶的抗疲劳作用进行相关生理指标研究。结果:雪茶提取物显著增加了小鼠的游泳时间,血液和肝脏中各个指标的变化具有良性效果。结论:雪茶提取物有提高小鼠的抗疲劳作用的效果,并在一定浓度范围内呈剂量依赖性显著改善小鼠抗疲劳能力。展开更多
基金Fundamental Research Funds for the Central Universities,China(No.16D110523)
文摘The present study aimed at isolating,purifying,characterizing the structure and estimating the anti-inflammatory activity of a polysaccharide named THSA which was obtained from Thamnolia subuliformis(Ehrh.) W.Culb(T.subuliformis).A newheteroglycan THSA was gained by hot water extraction and further continuously purification through Sephadex G-75 and Superdex 200 column chromatography.The structural characteristics of THSA were primarily investigated by molecular mass estimation,monosaccharide composition analysis and Fourier transforminfrared spectroscopy(FT-IR).The anti-inflammatory effects of THSA was also estimated in lipopolysaccharide(LPS)-stimulated mouse macrophages cell line RAW264.7 through the method of reverse transcription polymerase chain reaction(RT-PCR) and quantitative real-time PCR.The results indicate that THSA is composed of Glc,Gal,Man in the ratio 1:2.1:6.16,with average molecular weight of about 12.4 k Da,and significantly inhibits the mRNA expression of tumor necrosis factor alpha(TNF-α),IL-6 at100 or 1 000 μg/m L and increases the anti-inflammatory cytokine level of IL-10 at 100 μg/m L.It indicates that polysaecharide THSA has strong anti-inflammatory activity.
文摘A new phenolic compound, thamnolin (1), was isolated from the extract of Thamnolia vermicularis. Its structure was determined as 6-tricosyl-2,4-dimethoxy-phenol by spectral methods.
文摘探讨雪茶提取物[Extractive of Thamnolia vermicularis(ACH)Asahina]的抗疲劳作用效果。方法:取小鼠,随机分组,实验设高、低剂量组分、空白组、阳性药物组。雪茶提取物应用灌胃给药方法,通过各组小鼠负重游泳实验,对雪茶的抗疲劳作用进行物理性研究;通过测试小鼠游泳后和静置一定时间后血液中血糖变化,血乳酸变化,肝糖原含量及血尿素含量,对雪茶的抗疲劳作用进行相关生理指标研究。结果:雪茶提取物显著增加了小鼠的游泳时间,血液和肝脏中各个指标的变化具有良性效果。结论:雪茶提取物有提高小鼠的抗疲劳作用的效果,并在一定浓度范围内呈剂量依赖性显著改善小鼠抗疲劳能力。
