Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundan...Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies.展开更多
Effective vegetation reconstruction plays a vital role in the restoration of desert ecosystems.However,in reconstruction of different vegetation types,the community characteristics,assembly processes,and functions of ...Effective vegetation reconstruction plays a vital role in the restoration of desert ecosystems.However,in reconstruction of different vegetation types,the community characteristics,assembly processes,and functions of different soil microbial taxa under environmental changes are still disputed,which limits the understanding of the sustainability of desert restoration.Hence,we investigated the soil microbial community characteristics and functional attributes of grassland desert(GD),desert steppe(DS),typical steppe(TS),and artificial forest(AF)in the Mu Us Desert,China.Our findings confirmed the geographical conservation of soil microbial composition but highlighted decreased microbial diversity in TS.Meanwhile,the abundance of rare taxa and microbial community stability in TS improved.Heterogeneous and homogeneous selection determined the assembly of rare and abundant bacterial taxa,respectively,with both being significantly influenced by soil moisture.In contrast,fungal communities displayed stochastic processes and exhibited sensitivity to soil nutrient conditions.Furthermore,our investigation revealed a noteworthy augmentation in bacterial metabolic functionality in TS,aligning with improved vegetation restoration and the assemblage of abundant bacterial taxa.However,within nutrient-limited soils(GD,DS,and AF),the assembly dynamics of rare fungal taxa assumed a prominent role in augmenting their metabolic capacity and adaptability to desert ecosystems.These results highlighted the variations in the assembly processes and metabolic functions of soil microorganisms during vegetation reestablishment and provided corresponding theoretical support for anthropogenic revegetation of desert ecosystems.展开更多
phoD and pqqC gene occurrence in bacteria allows them to mobilize phosphorus(P)by mineralizing organic P(Po)and solubilizing inorganic P(Pi),respectively.Community characteristics of phoD-and pqqC-harboring bacteria(p...phoD and pqqC gene occurrence in bacteria allows them to mobilize phosphorus(P)by mineralizing organic P(Po)and solubilizing inorganic P(Pi),respectively.Community characteristics of phoD-and pqqC-harboring bacteria(phoD-and pqqC-HB,respectively)mediate P cycling.However,whether the microbial community assembly and keystone taxa of phoD-and pqqC-HB regulate P availability and distinct regulatory pathways between these two genes remain unclear.In this study,soil microbial community characteristics and P availability were investigated in four long-term(38-year)fertilization regimes:control with no fertilizer(CK),P fertilizer(PF),nitrogen(N)and P fertilizers(NP),and N fertilizer,P fertilizer,and manure(NPM).The N addition treatments(NP and NPM)significantly changed the community composition and increased the abundances of phoD-and pqqC-HB compared to the no-N addition treatments(CK and PF).Stochastic processes dominated the community assembly of both phoD-and pqqC-HB,and the relative contributions of stochasticity increased with N addition.Furthermore,the N addition treatments resulted in greater network complexity and higher abundances of keystone taxa of phoD-and pqqC-HB compared to those of the no-N addition treatments.The keystone taxa implicated in P cycling were also associated with carbon(C)and N cycling processes.Microbial community composition and assembly processes were the main factors driving labile Pi for phoD-HB,whereas keystone taxa contributed the most to labile Pi for pqqC-HB.These results emphasize that distinct mechanisms of phoD-and pqqC-HB regulate P availability under fertilization management and underline the significance of microbial community assembly and keystone taxa in soil ecological functions,offering fresh perspectives on comprehending the biological processes facilitated by microorganisms in enhancing soil quality.展开更多
Earthworm gut microbiome can significantly influence soil microbial community and functions.However,how earthworms affect the abundant,intermediate,and rare soil bacterial taxa and subsequently regulate soil multifunc...Earthworm gut microbiome can significantly influence soil microbial community and functions.However,how earthworms affect the abundant,intermediate,and rare soil bacterial taxa and subsequently regulate soil multifunctionality remains poorly understood.In this study,we investigated bacteria composition and functional gene traits with and without earthworm addition in low-nutrient soil.Our results show that earthworm addition enhanced soil multifunctionality,including organic carbon,nitrogen,and phosphorus mineralization.Compared to other groups,abundant taxa in earthworm-treated soil exhibited higher 16S rRNA operon copy numbers,copiotroph/oligotroph ratios,niche width,and network efficiency,suggesting a greater competitive capacity for resource acquisition.We identified a core set of persistent abundant taxa genera(11 genera)in earthworm-treated soil,which persisted throughout the incubation period,and were notably dominant among abundant taxa in the earthworm gut(67.1%−79.2%).Furthermore,structural equation modeling revealed that gut-associated abundant taxa strongly influenced the composition of soil abundant taxa and persistent core abundant taxa genera,which in turn increased soil r-strategists and enhanced multifunctionality.Overall,our findings provide new insights into the ecological strategies of different soil taxa in response to earthworm addition and highlight the role of earthworm gut microbiome in adapting to nutrient-poor environments.展开更多
Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communiti...Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand.展开更多
The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Z...The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology.展开更多
Research and protection of biodiversity is generally started with species recognizing guided by achievement of cataloguing and illustration obtained through in situ investigation data accumulation and literature learn...Research and protection of biodiversity is generally started with species recognizing guided by achievement of cataloguing and illustration obtained through in situ investigation data accumulation and literature learning. Facing the shortage of taxonomist, it is getting harder to maintain important specimen collections and handle loans. The West Pacific Ocean has been operating as a center of the origin of global marine biodiversity result of the richest species diversity of marine taxa found in these waters. The present work is a compilation and summary of systematics, species diversity and new taxa of mesozooplankton major group known in West Pacific Ocean and its marginal seas （0°-45°N, 105°-152°E） according to 6 203 mesozooplankton samples acquired from 701 stations during 1965 and 2008. A total of 2 657 species belonging to 686 genera and 206 families of 10 mesozooplankton groups have been found through taxonomic identification and document consulting. In details, 697 species from 251 genera of 99 families belong to the Medusozoa, 59 species from 22 genera of 12 families to the Ctenophora, 85 species from 36 genera of 14 families to the pelagic Mollusca （Pteropoda and Heteropoda）, 416 species from 91 genera of 8 families to the Ostracoda, 908 species from 156 genera of 51 families to the Copepoda, 202 species from 60 genera of 4 families to the Mysidacea, 56 species from 8 genera of 2 families to the Euphausiacea, 105 species from 23 genera of 8 families to the Decapoda, 48 species from 13 genera of 5 families to the Chaetognatha and 81 species from 26 genera of 5 families to the Tunicata. The dominant species of each group are enumerated. Moreover, 2 new species of Medusozoa, Tubulariidae, Ectopleura, 1 new species of Medusozoa, Protiaridae, Halitiarella, 1 new genus and 1 new species of Medusozoa, Corymorphidae are established. An amount of 806 species are expanded with an increase of 43.5% on the basis of 1 852 species recorded before 2008, including 1.4% increase from tropical sea around equator, 4.0% from the frigid water in high latitude region, and 3.0% of bathypelagic-associated waters. The authors also summarized future prospects into five major areas in marine mesozooplankton research in China. Such information of qualitative phyletic classification could be of high relevance to studies on biodiversity and biogeography of marine mesozooplankton, especially for monographs contributed to make an overall and systematic conclusion on the species of marine life in China after 2008.展开更多
Eight new taxa of Euglenophyta are described in this paper. They were collected from severalprovinces in China and respectively named Euglena allorgei var. exsulcata. E pisciformis var. globosa, E.tortiliS, Lepocincli...Eight new taxa of Euglenophyta are described in this paper. They were collected from severalprovinces in China and respectively named Euglena allorgei var. exsulcata. E pisciformis var. globosa, E.tortiliS, Lepocinclis glabra var. papillata, Phacus pisiformis, Ph.strombuliformis,Ph.trimarginatus var.truncatus and Astasia angusta.展开更多
The Cameroonian flora has a very high biodiversity that remains largely unexplored. It contains many grasses that can be used for sports lawns, but are currently underutilized. In order to contribute to the improvemen...The Cameroonian flora has a very high biodiversity that remains largely unexplored. It contains many grasses that can be used for sports lawns, but are currently underutilized. In order to contribute to the improvement of the quality of sports lawns, nine local grass taxa from the agro-ecological zone with bimodal rainfall in Cameroon were evaluated for their potential for sporting use in natural lawns. The method of the Study and Control Group for Varieties and Seeds was used to evaluate the sports lawn characteristics of the taxa. The resistance to trampling and pulling, the density of the lawn, and the aesthetic appearance, which are factors in calculating the sports index, were monitored at two sites, including the experimental space at the University of Yaounde 1 in the Center region and Kagnol 2 in the East region, from January 2017 to January 2020. The monthly lawn characteristic data allowed for the differentiation between the means and the determination of the sports index of the taxa compared to commercialized lawn varieties. The sports index of the evaluated taxa varied: Cynodon dactylon (6.989), Eleusine indica (6.338), Sporobolus natalensis (6.301), Cynodon cf Cynodon dactylon (6.257), Eragrostis tremula (5.939), Sporobolus pyramidalis (5.583), Agrostis rupestris (5.335), Axonopus compressus (4.991), and Digitaria sp. (4.544). These results show that these grass taxa have potential for sporting use in lawn mixtures at different levels of sports disciplines.展开更多
The understanding of plant-microbe interactions in terms of core and/or keystone taxa is crucial for enhancing plant stress tolerance.Nevertheless,the investigation of this key component of microbiome associated with ...The understanding of plant-microbe interactions in terms of core and/or keystone taxa is crucial for enhancing plant stress tolerance.Nevertheless,the investigation of this key component of microbiome associated with plants thriving in extreme environments,like non-mycorrhizal sedges on the Qinghai-Tibet Plateau,has been relatively limited.In this study,we employed frequency-abundance methods and molecular ecological network analysis to identify the core and keystone taxa of fungi and bacteria in both rhizosphere soil and root endosphere of Carex cepillacea.The results revealed a substantial number of unique taxa in both core and keystone taxa,with Sphingomonas and Gibberella representing core taxa,while Nocardioides and Truncatella serve as the keystone taxa.Specifically,there was a considerably higher proportion of exclusive taxa in the keystone taxa(bacteria:48.8%,fungi:55.4%)compared to that observed in core taxa(bacteria:16.3%,fungi:10.7%).Regarding microorganisms inhabiting rhizosphere soil,total nitrogen(TN)primarily influenced the assembly of core communities while available phosphorus(AP)played a major role in shaping the keystone communities.Within the root endosphere,both the core and keystone microbial communities were significantly more influenced by soil carbon and TN nutrients compared to other factors.It is noteworthy that certain“common core”taxa,such as Actinoplanes,Blastococcus,Penicillium,and Fusarium,exhibited high interconnectedness within the entire microbiome network.Considering the contribution of keystone taxa is significantly enhanced when they are part of the core taxa,these findings can provide a foundation for the development of microbial formulations based on key constituents of the microbiome.展开更多
Microplastics and heavy metal contamination poses major threats to soil function and food security;however,their synergistic effects remain largely unclear.This study investigated the effects of single or combined add...Microplastics and heavy metal contamination poses major threats to soil function and food security;however,their synergistic effects remain largely unclear.This study investigated the effects of single or combined addition of polyethylene(PE)microplastic(1%w/w)and cadmium(Cd;1.5 and 5 mg kg^(-1))on functional microbial communities in the wheat rhizosphere soil.We observed that the biomass of wheat increased by 142.44%under high doses of Cd addition.The bacterial alpha diversity in wheat bulk soil reduced by 37.34%–37.83%with the combined addition of microplastic and Cd.The addition of microplastic reduced the relative abundance of Proteus involved in nitrogen fixation by 19.93%,while the relative abundance of Proteus and Actinobacteria involved in nitrogen cycling increased with the increase of Cd concentration,increasing by 27.96%–37.37%and 51.14%–55.04%,respectively.FAPROTAX analysis revealed that increasing Cd concentration promoted the abundance of functional bacterial communities involved in nitrification/denitrification and nitrate/nitrite respiration in rhizosphere soil.A FunGuild analysis showed that the synergy of PE-microplastics and Cd increased the abundance of saprophytic fungi,suggesting an enhanced degradation function.Our findings provide new knowledge on the effects of microplastics and heavy metals on soil microorganisms and functional microbial communities in agricultural soil.展开更多
Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunit...Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunity supported the strength of SBF.•Stochastic ratio was the most important predictor for the strength of SBF.It is known that soil microbial communities are intricately linked to multiple ecosystem functions and can maintain the relationship between soil biodiversity and multifunctionality(SBF)under environmental stresses.However,the relative contributions and driving forces of abundant and rare taxa within the communities in maintaining soil biodiversity-multifunctionality relationship under pollution stresses are still unclear.Here,we conducted microcosm experiments to estimate the importance of soil abundant and rare taxa in predicting these relationships under heavy metal mercury(Hg)stress in paired paddy and upland fields.The results revealed that the diversity of abundant taxa,rather than rare taxa,was positively related to multifunctionality,with the abundant subcommunity tending to maintain a larger proportion of soil functions including chitin degradation,protein degradation,and phosphorus mineralization.Soil multitrophic network complexity consisting of abundant species showed positive correlations with biodiversity and multifunctionality,and supported the strength of SBF within a network complexity range.Stochastic assembly processes of the abundant subcommunity were positively correlated with the strength of SBF,although stochastic processes decreased the biodiversity and the multifunctionality,respectively.After simultaneously accounting for multiple factors on the strength of SBF,we found that the stochastic community assembly ratio of abundant taxa was the most important predictor for SBF strength under Hg stress.Our results highlight the importance of abundant taxa in supporting soil multifunctionality,and elucidate the linkages between community assembly,network complexity and SBF relationship under environmental stresses.展开更多
Core taxa play an important role in regulating soil carbon metabolism.Ecological cluster with oligotrophic made key contributions to soil carbon metabolism.Microbial cluster characteristics link microorganisms to carb...Core taxa play an important role in regulating soil carbon metabolism.Ecological cluster with oligotrophic made key contributions to soil carbon metabolism.Microbial cluster characteristics link microorganisms to carbon metabolism.Characterizing the ecological roles of core soil microbial species in soil carbon metabolism is critically important for enhancing carbon sequestration in agricultural systems;however,no studies to date have determined the effects of core soil microbial taxa on carbon metabolism under various long-term fertilization practices.Here,we collected soil samples from field plots that had been subjected to different fertilization practices for nearly 30 years and examined the long-term effects of fertilization on the preferences of core soil bacterial taxa for different carbon sources.We also examined the relative contribution of core soil bacterial taxa in utilization of different carbon source types in Biolog Eco microplates.Long-term fertilization treatment had a significant effect on soil properties and bacterial community structure.The core taxa were closely related to soil carbon source utilization.The co-occurrence network showed that the major ecological clusters containing core taxa made key contributions to soil carbon source utilization.The organic fertilization increased the abundance of a core cluster with a low weighted average rrn copy number.This ecological cluster was the most important factor affecting soil carbon source utilization even among soil physicochemical factors considered.Our findings indicate that core taxa characterized by oligotrophic bacteria have a major effect on carbon source utilization in Ultisols.展开更多
Hainan Province is situated at the northern edge of the Asian tropics,serving as China’s only tropical province.The province harbors exceptional biodiversity due to its unique geography and climate,particularly in fu...Hainan Province is situated at the northern edge of the Asian tropics,serving as China’s only tropical province.The province harbors exceptional biodiversity due to its unique geography and climate,particularly in fungal diversity.However,previous mycological surveys in Hainan are predominantly focused on central forested regions,while coastal ecosystems are largely overlooked.This study reports the first discovery of the genus Calocybella in China,with one new species and one new record for China collected from coastal Casuarina forests.Calocybella hainanensis sp.nov.is characterized by its ginger-yellow to yellowish-brown pileus,pale yellow lamellae,and small,oblong-ellipsoid basidiospores(4.9–5.5×2.3–3.1μm)with faint ornamentation and inamyloid reaction.The newly recorded species,Calocybella sribuabanensis,exhibits gregarious growth,yellowish-brown pileus,densely arranged lamellae,and ellipsoid,faintly ornamented,inamyloid basidiospores.Phylogenetic analyses based on ITS sequences reveal that C.hainanensis forms a unique evolutionary lineage within Calocybella,but it shows unresolved affinities to other taxa.C.sribuabanensis forms a sister lineage with the Indian species Calocybella dicholamellata.展开更多
基金supported by the National Natural Science Foundation of China(No.42007128)the Fundamental Research Funds for the Central Universities(No.2024QNYL30)the Graduate Research and Practice Projects of Minzu University of China(No.SZKY2024034).
文摘Traditional studies of microbial succession under iron-carbon composite(Fe-C)amendment application have focused on the entire microbial community,with limited attention to the responses and ecological roles of abundant or rare taxa.Herein,a 90-day microcosm incubation was conducted to investigate the effects of three Fe-C amendments,including Fe_(3)O_(4)-modified biochar(FeC-B),ferrihydrite-natural humic acid(FeC-N),and ferrihydrite-synthetic humic-like acid(FeC-S),on distribution patterns,assembly processes,and ecological functions of both abundant and rare subcommunities.Our results showed that Fe-C amendments significantly affected theα-diversity of rare taxa,particularly under FeC-B treatment,with minimal impact on abundant taxa.Fe-C amendments also reshaped the community structures of both groups.Rare taxa,representing 63.9%of Operational Taxonomic Unit(OTU)richness but only 1.6%of total abundance,played a key role in community diversity and were more susceptible to Fe-C amendments.Certain rare taxa transitioned to abundant status,demonstrating their potential as a microbial seed bank.Abundant taxa were positioned more centrally within the networks,and Fe-C applications promoted cooperative interactions between abundant and rare species.Deterministic processes dominated the assembly of the rare subcommunity,while stochastic processes primarily influenced the abundant bacterial community.Fe-C amendments reduced community differentiation among rare taxa while increasing variability among abundant groups.Functional diversity of rare groups surpassed that of abundant groups,with notable enhancement in nitrogen cycling-related genes under Fe-C treatments.This study highlights the complementary roles of abundant and rare taxa in soil remediation,providing insights for optimizing remediation strategies.
基金supported by the National Natural Science Foundation of China(No.42007428)the National Forage Industry Technology System Program of China(No.CARS34)+1 种基金the Key Research and Development Program of Shaanxi,China(No.2022SF-285)Shaanxi Province Forestry Science and Technology Innovation Program,China(No.SXLK2022-02-14)。
文摘Effective vegetation reconstruction plays a vital role in the restoration of desert ecosystems.However,in reconstruction of different vegetation types,the community characteristics,assembly processes,and functions of different soil microbial taxa under environmental changes are still disputed,which limits the understanding of the sustainability of desert restoration.Hence,we investigated the soil microbial community characteristics and functional attributes of grassland desert(GD),desert steppe(DS),typical steppe(TS),and artificial forest(AF)in the Mu Us Desert,China.Our findings confirmed the geographical conservation of soil microbial composition but highlighted decreased microbial diversity in TS.Meanwhile,the abundance of rare taxa and microbial community stability in TS improved.Heterogeneous and homogeneous selection determined the assembly of rare and abundant bacterial taxa,respectively,with both being significantly influenced by soil moisture.In contrast,fungal communities displayed stochastic processes and exhibited sensitivity to soil nutrient conditions.Furthermore,our investigation revealed a noteworthy augmentation in bacterial metabolic functionality in TS,aligning with improved vegetation restoration and the assemblage of abundant bacterial taxa.However,within nutrient-limited soils(GD,DS,and AF),the assembly dynamics of rare fungal taxa assumed a prominent role in augmenting their metabolic capacity and adaptability to desert ecosystems.These results highlighted the variations in the assembly processes and metabolic functions of soil microorganisms during vegetation reestablishment and provided corresponding theoretical support for anthropogenic revegetation of desert ecosystems.
基金supported by the National Key Research and Development Program of China(No.2022YFD 1901601)the National Natural Science Foundation of China(No.42107360)the Fundamental Research Funds for the Central Universities,China(No.2452021032).
文摘phoD and pqqC gene occurrence in bacteria allows them to mobilize phosphorus(P)by mineralizing organic P(Po)and solubilizing inorganic P(Pi),respectively.Community characteristics of phoD-and pqqC-harboring bacteria(phoD-and pqqC-HB,respectively)mediate P cycling.However,whether the microbial community assembly and keystone taxa of phoD-and pqqC-HB regulate P availability and distinct regulatory pathways between these two genes remain unclear.In this study,soil microbial community characteristics and P availability were investigated in four long-term(38-year)fertilization regimes:control with no fertilizer(CK),P fertilizer(PF),nitrogen(N)and P fertilizers(NP),and N fertilizer,P fertilizer,and manure(NPM).The N addition treatments(NP and NPM)significantly changed the community composition and increased the abundances of phoD-and pqqC-HB compared to the no-N addition treatments(CK and PF).Stochastic processes dominated the community assembly of both phoD-and pqqC-HB,and the relative contributions of stochasticity increased with N addition.Furthermore,the N addition treatments resulted in greater network complexity and higher abundances of keystone taxa of phoD-and pqqC-HB compared to those of the no-N addition treatments.The keystone taxa implicated in P cycling were also associated with carbon(C)and N cycling processes.Microbial community composition and assembly processes were the main factors driving labile Pi for phoD-HB,whereas keystone taxa contributed the most to labile Pi for pqqC-HB.These results emphasize that distinct mechanisms of phoD-and pqqC-HB regulate P availability under fertilization management and underline the significance of microbial community assembly and keystone taxa in soil ecological functions,offering fresh perspectives on comprehending the biological processes facilitated by microorganisms in enhancing soil quality.
基金supported by the National Natural Science Foundation of China(Grant Nos.42077088,42407447)Zhejiang Province“Agriculture,Rural Areas,Rural People and Nine Institutions”Science and Technology Collaboration Program,China Postdoctoral Science Foundation(Certificate Number:2023M743418)the Key Project of Science and Technology Innovation in Ningbo City(Grant No.2022Z169).
文摘Earthworm gut microbiome can significantly influence soil microbial community and functions.However,how earthworms affect the abundant,intermediate,and rare soil bacterial taxa and subsequently regulate soil multifunctionality remains poorly understood.In this study,we investigated bacteria composition and functional gene traits with and without earthworm addition in low-nutrient soil.Our results show that earthworm addition enhanced soil multifunctionality,including organic carbon,nitrogen,and phosphorus mineralization.Compared to other groups,abundant taxa in earthworm-treated soil exhibited higher 16S rRNA operon copy numbers,copiotroph/oligotroph ratios,niche width,and network efficiency,suggesting a greater competitive capacity for resource acquisition.We identified a core set of persistent abundant taxa genera(11 genera)in earthworm-treated soil,which persisted throughout the incubation period,and were notably dominant among abundant taxa in the earthworm gut(67.1%−79.2%).Furthermore,structural equation modeling revealed that gut-associated abundant taxa strongly influenced the composition of soil abundant taxa and persistent core abundant taxa genera,which in turn increased soil r-strategists and enhanced multifunctionality.Overall,our findings provide new insights into the ecological strategies of different soil taxa in response to earthworm addition and highlight the role of earthworm gut microbiome in adapting to nutrient-poor environments.
基金the Sino-German Cooperation Forestry Major Scientific Research Project(zdczhz2021ky09)the National Natural Science Foundation of China(31971487 and 42277245).
文摘Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand.
基金supported by the Cooperation Project of Luzhou Laojiao Co.,Ltd.Sichuan University (21H0997)。
文摘The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology.
基金The National Natural Science Foundation of China under contract Nos 41506217 and 41506136the Basic Research of the National Department of Science and Technology under contract Nos GASI-01-02-04 and 201105022-2
文摘Research and protection of biodiversity is generally started with species recognizing guided by achievement of cataloguing and illustration obtained through in situ investigation data accumulation and literature learning. Facing the shortage of taxonomist, it is getting harder to maintain important specimen collections and handle loans. The West Pacific Ocean has been operating as a center of the origin of global marine biodiversity result of the richest species diversity of marine taxa found in these waters. The present work is a compilation and summary of systematics, species diversity and new taxa of mesozooplankton major group known in West Pacific Ocean and its marginal seas （0°-45°N, 105°-152°E） according to 6 203 mesozooplankton samples acquired from 701 stations during 1965 and 2008. A total of 2 657 species belonging to 686 genera and 206 families of 10 mesozooplankton groups have been found through taxonomic identification and document consulting. In details, 697 species from 251 genera of 99 families belong to the Medusozoa, 59 species from 22 genera of 12 families to the Ctenophora, 85 species from 36 genera of 14 families to the pelagic Mollusca （Pteropoda and Heteropoda）, 416 species from 91 genera of 8 families to the Ostracoda, 908 species from 156 genera of 51 families to the Copepoda, 202 species from 60 genera of 4 families to the Mysidacea, 56 species from 8 genera of 2 families to the Euphausiacea, 105 species from 23 genera of 8 families to the Decapoda, 48 species from 13 genera of 5 families to the Chaetognatha and 81 species from 26 genera of 5 families to the Tunicata. The dominant species of each group are enumerated. Moreover, 2 new species of Medusozoa, Tubulariidae, Ectopleura, 1 new species of Medusozoa, Protiaridae, Halitiarella, 1 new genus and 1 new species of Medusozoa, Corymorphidae are established. An amount of 806 species are expanded with an increase of 43.5% on the basis of 1 852 species recorded before 2008, including 1.4% increase from tropical sea around equator, 4.0% from the frigid water in high latitude region, and 3.0% of bathypelagic-associated waters. The authors also summarized future prospects into five major areas in marine mesozooplankton research in China. Such information of qualitative phyletic classification could be of high relevance to studies on biodiversity and biogeography of marine mesozooplankton, especially for monographs contributed to make an overall and systematic conclusion on the species of marine life in China after 2008.
基金The project supported by National Natural Science Foundation of China
文摘Eight new taxa of Euglenophyta are described in this paper. They were collected from severalprovinces in China and respectively named Euglena allorgei var. exsulcata. E pisciformis var. globosa, E.tortiliS, Lepocinclis glabra var. papillata, Phacus pisiformis, Ph.strombuliformis,Ph.trimarginatus var.truncatus and Astasia angusta.
文摘The Cameroonian flora has a very high biodiversity that remains largely unexplored. It contains many grasses that can be used for sports lawns, but are currently underutilized. In order to contribute to the improvement of the quality of sports lawns, nine local grass taxa from the agro-ecological zone with bimodal rainfall in Cameroon were evaluated for their potential for sporting use in natural lawns. The method of the Study and Control Group for Varieties and Seeds was used to evaluate the sports lawn characteristics of the taxa. The resistance to trampling and pulling, the density of the lawn, and the aesthetic appearance, which are factors in calculating the sports index, were monitored at two sites, including the experimental space at the University of Yaounde 1 in the Center region and Kagnol 2 in the East region, from January 2017 to January 2020. The monthly lawn characteristic data allowed for the differentiation between the means and the determination of the sports index of the taxa compared to commercialized lawn varieties. The sports index of the evaluated taxa varied: Cynodon dactylon (6.989), Eleusine indica (6.338), Sporobolus natalensis (6.301), Cynodon cf Cynodon dactylon (6.257), Eragrostis tremula (5.939), Sporobolus pyramidalis (5.583), Agrostis rupestris (5.335), Axonopus compressus (4.991), and Digitaria sp. (4.544). These results show that these grass taxa have potential for sporting use in lawn mixtures at different levels of sports disciplines.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program(Grant No.2019QZKK0308)the National Natural Science Foundation of China(Grant No.41877049).
文摘The understanding of plant-microbe interactions in terms of core and/or keystone taxa is crucial for enhancing plant stress tolerance.Nevertheless,the investigation of this key component of microbiome associated with plants thriving in extreme environments,like non-mycorrhizal sedges on the Qinghai-Tibet Plateau,has been relatively limited.In this study,we employed frequency-abundance methods and molecular ecological network analysis to identify the core and keystone taxa of fungi and bacteria in both rhizosphere soil and root endosphere of Carex cepillacea.The results revealed a substantial number of unique taxa in both core and keystone taxa,with Sphingomonas and Gibberella representing core taxa,while Nocardioides and Truncatella serve as the keystone taxa.Specifically,there was a considerably higher proportion of exclusive taxa in the keystone taxa(bacteria:48.8%,fungi:55.4%)compared to that observed in core taxa(bacteria:16.3%,fungi:10.7%).Regarding microorganisms inhabiting rhizosphere soil,total nitrogen(TN)primarily influenced the assembly of core communities while available phosphorus(AP)played a major role in shaping the keystone communities.Within the root endosphere,both the core and keystone microbial communities were significantly more influenced by soil carbon and TN nutrients compared to other factors.It is noteworthy that certain“common core”taxa,such as Actinoplanes,Blastococcus,Penicillium,and Fusarium,exhibited high interconnectedness within the entire microbiome network.Considering the contribution of keystone taxa is significantly enhanced when they are part of the core taxa,these findings can provide a foundation for the development of microbial formulations based on key constituents of the microbiome.
基金supported by the Ningbo Science and Technology Bureau(Grant No.2022S103)the National Natural Science Foundation of China(Grant Nos.42107341,42307420)+1 种基金the UK Natural Environment Research Council and the Global Challenges Research Fund(Grant No.NE/V005871/1)the K.C.Wong Magna Fund at Ningbo University.
文摘Microplastics and heavy metal contamination poses major threats to soil function and food security;however,their synergistic effects remain largely unclear.This study investigated the effects of single or combined addition of polyethylene(PE)microplastic(1%w/w)and cadmium(Cd;1.5 and 5 mg kg^(-1))on functional microbial communities in the wheat rhizosphere soil.We observed that the biomass of wheat increased by 142.44%under high doses of Cd addition.The bacterial alpha diversity in wheat bulk soil reduced by 37.34%–37.83%with the combined addition of microplastic and Cd.The addition of microplastic reduced the relative abundance of Proteus involved in nitrogen fixation by 19.93%,while the relative abundance of Proteus and Actinobacteria involved in nitrogen cycling increased with the increase of Cd concentration,increasing by 27.96%–37.37%and 51.14%–55.04%,respectively.FAPROTAX analysis revealed that increasing Cd concentration promoted the abundance of functional bacterial communities involved in nitrification/denitrification and nitrate/nitrite respiration in rhizosphere soil.A FunGuild analysis showed that the synergy of PE-microplastics and Cd increased the abundance of saprophytic fungi,suggesting an enhanced degradation function.Our findings provide new knowledge on the effects of microplastics and heavy metals on soil microorganisms and functional microbial communities in agricultural soil.
基金financially supported by the National Natural Science Foundation of China(42177022 and 41877120)Natural Science Foundation of Hubei Province,China(2020CFA013).
文摘Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunity supported the strength of SBF.•Stochastic ratio was the most important predictor for the strength of SBF.It is known that soil microbial communities are intricately linked to multiple ecosystem functions and can maintain the relationship between soil biodiversity and multifunctionality(SBF)under environmental stresses.However,the relative contributions and driving forces of abundant and rare taxa within the communities in maintaining soil biodiversity-multifunctionality relationship under pollution stresses are still unclear.Here,we conducted microcosm experiments to estimate the importance of soil abundant and rare taxa in predicting these relationships under heavy metal mercury(Hg)stress in paired paddy and upland fields.The results revealed that the diversity of abundant taxa,rather than rare taxa,was positively related to multifunctionality,with the abundant subcommunity tending to maintain a larger proportion of soil functions including chitin degradation,protein degradation,and phosphorus mineralization.Soil multitrophic network complexity consisting of abundant species showed positive correlations with biodiversity and multifunctionality,and supported the strength of SBF within a network complexity range.Stochastic assembly processes of the abundant subcommunity were positively correlated with the strength of SBF,although stochastic processes decreased the biodiversity and the multifunctionality,respectively.After simultaneously accounting for multiple factors on the strength of SBF,we found that the stochastic community assembly ratio of abundant taxa was the most important predictor for SBF strength under Hg stress.Our results highlight the importance of abundant taxa in supporting soil multifunctionality,and elucidate the linkages between community assembly,network complexity and SBF relationship under environmental stresses.
基金supported by the National Key Research and Development Program of China(Grant No.2021YFD1901201-05)the National Natural Science Foundation of China(Grant No.41661052)+2 种基金Agricultural Projects Jiangxi Province(Grant No.JXNK202307-01)Special Program for Basic Research and Talent Training of Jiangxi Academy of Agricultural Sciences(Grant No.JXSNKYJCRC202301)the Outstanding Youth Research Project of Anhui Province(Grant No.2022AH030015).
文摘Core taxa play an important role in regulating soil carbon metabolism.Ecological cluster with oligotrophic made key contributions to soil carbon metabolism.Microbial cluster characteristics link microorganisms to carbon metabolism.Characterizing the ecological roles of core soil microbial species in soil carbon metabolism is critically important for enhancing carbon sequestration in agricultural systems;however,no studies to date have determined the effects of core soil microbial taxa on carbon metabolism under various long-term fertilization practices.Here,we collected soil samples from field plots that had been subjected to different fertilization practices for nearly 30 years and examined the long-term effects of fertilization on the preferences of core soil bacterial taxa for different carbon sources.We also examined the relative contribution of core soil bacterial taxa in utilization of different carbon source types in Biolog Eco microplates.Long-term fertilization treatment had a significant effect on soil properties and bacterial community structure.The core taxa were closely related to soil carbon source utilization.The co-occurrence network showed that the major ecological clusters containing core taxa made key contributions to soil carbon source utilization.The organic fertilization increased the abundance of a core cluster with a low weighted average rrn copy number.This ecological cluster was the most important factor affecting soil carbon source utilization even among soil physicochemical factors considered.Our findings indicate that core taxa characterized by oligotrophic bacteria have a major effect on carbon source utilization in Ultisols.
基金supported by the National Science Foundation of China(32260005,32470008)the Hainan Province Science and Technology Special Fund(ZDYF2024SHFZ129)+1 种基金the Hainan Provincial Natural Science Foundation(323MS044)the Scientific Research Project of Hainan Higher Education Institutions(Hnky2023ZD-8).
文摘Hainan Province is situated at the northern edge of the Asian tropics,serving as China’s only tropical province.The province harbors exceptional biodiversity due to its unique geography and climate,particularly in fungal diversity.However,previous mycological surveys in Hainan are predominantly focused on central forested regions,while coastal ecosystems are largely overlooked.This study reports the first discovery of the genus Calocybella in China,with one new species and one new record for China collected from coastal Casuarina forests.Calocybella hainanensis sp.nov.is characterized by its ginger-yellow to yellowish-brown pileus,pale yellow lamellae,and small,oblong-ellipsoid basidiospores(4.9–5.5×2.3–3.1μm)with faint ornamentation and inamyloid reaction.The newly recorded species,Calocybella sribuabanensis,exhibits gregarious growth,yellowish-brown pileus,densely arranged lamellae,and ellipsoid,faintly ornamented,inamyloid basidiospores.Phylogenetic analyses based on ITS sequences reveal that C.hainanensis forms a unique evolutionary lineage within Calocybella,but it shows unresolved affinities to other taxa.C.sribuabanensis forms a sister lineage with the Indian species Calocybella dicholamellata.
