摘要
The understanding of plant-microbe interactions in terms of core and/or keystone taxa is crucial for enhancing plant stress tolerance.Nevertheless,the investigation of this key component of microbiome associated with plants thriving in extreme environments,like non-mycorrhizal sedges on the Qinghai-Tibet Plateau,has been relatively limited.In this study,we employed frequency-abundance methods and molecular ecological network analysis to identify the core and keystone taxa of fungi and bacteria in both rhizosphere soil and root endosphere of Carex cepillacea.The results revealed a substantial number of unique taxa in both core and keystone taxa,with Sphingomonas and Gibberella representing core taxa,while Nocardioides and Truncatella serve as the keystone taxa.Specifically,there was a considerably higher proportion of exclusive taxa in the keystone taxa(bacteria:48.8%,fungi:55.4%)compared to that observed in core taxa(bacteria:16.3%,fungi:10.7%).Regarding microorganisms inhabiting rhizosphere soil,total nitrogen(TN)primarily influenced the assembly of core communities while available phosphorus(AP)played a major role in shaping the keystone communities.Within the root endosphere,both the core and keystone microbial communities were significantly more influenced by soil carbon and TN nutrients compared to other factors.It is noteworthy that certain“common core”taxa,such as Actinoplanes,Blastococcus,Penicillium,and Fusarium,exhibited high interconnectedness within the entire microbiome network.Considering the contribution of keystone taxa is significantly enhanced when they are part of the core taxa,these findings can provide a foundation for the development of microbial formulations based on key constituents of the microbiome.
基金
supported by the Second Tibetan Plateau Scientific Expedition and Research Program(Grant No.2019QZKK0308)
the National Natural Science Foundation of China(Grant No.41877049).
