CtBP-interacting protein(CtIP)is known for its multifaceted roles in DNA repair and genomic stability,directing the homologous recombination-mediated DNA double-stranded break repair pathway via DNA end resection,an e...CtBP-interacting protein(CtIP)is known for its multifaceted roles in DNA repair and genomic stability,directing the homologous recombination-mediated DNA double-stranded break repair pathway via DNA end resection,an essential error-free repair process vital for genome stability.Mammalian oocytes are highly prone to DNA damage accumulation due to prolonged G2/prophase arrest.Here,we explore the functions of CtIP in meiotic cell cycle regulation via a mouse oocyte model.Depletion of CtIP by siRNA injection results in delayed germinal vesicle breakdown and failed polar body extrusion.Mechanistically,CtIP deficiency increases DNA damage and decreases the expression and nuclear entry of CCNB1,resulting in marked impairment of meiotic resumption,which can be rescued by exogenous CCNB1 overexpression.Furthermore,depletion of CtIP disrupts microtubule-organizing centers coalescence at spindle poles as indicated by failed accumulation ofγ-tubulin,p-Aurora kinase A,Kif2A,and TPX2,leading to abnormal spindle assembly and prometaphase arrest.These results provide valuable insights into the important roles of CtIP in the G2/M checkpoint and spindle assembly in mouse oocyte meiotic cell cycle regulation.展开更多
One of the most important aims of this study is to improve the core of the current VVER reactors to achieve more burn-up(or more cycle length)and more intrinsic safety.It is an independent study on the Russian new pro...One of the most important aims of this study is to improve the core of the current VVER reactors to achieve more burn-up(or more cycle length)and more intrinsic safety.It is an independent study on the Russian new proposed FAs,called TVS-2M,which would be applied for the future advanced VVERs.Some important aspects of neutronics as well as thermal hydraulics investigations(and analysis)of the new type of Fas are conducted,and results are compared with the standards PWR CDBL.The TVS-2M FA contains gadolinium-oxide which is mixed with UO_(2)(for different Gd densities and U-235 enrichments which are given herein),but the core does not contain BARs.The new type TVS-2M Fas are modeled by the SARCS software package to find the PMAXS format for three states of CZP and HZP as well as HFP,and then the whole core is simulated by the PARCS code to investigate transient conditions.In addition,the WIMS-D5 code is suggested for steady core modeling including TVS-2M FAs and/or TVS FAs.Many neutronics aspects such as the first cycle length(first cycle burn up in terms of MWthd/kgU),the critical concentration of boric acid at the BOC as well as the cycle length,the axial,and radial power peaking factors,differential and integral worthy of the most reactive CPS-CRs,reactivity coefficients of the fuel,moderator,boric acid,and the under-moderation estimation of the core are conducted and benchmarked with the PWR CDBL.Specifically,the burn-up calculations indicate that the 45.6 d increase of the first cycle length(which corresponds to 1.18 MWthd/kgU increase of burnup)is the best improving aim of the new FA type called TVS-2M.Moreover,thermal-hydraulics core design criteria such as MDNBR(based on W3 correlation)and the maximum of fuel and clad temperatures(radially and axially),are investigated,and discussed based on the CDBL.展开更多
基金supported by National Natural Science Foundation of China(32570854)Science and Technology Program of Guangzhou,China(2023A03J0258)Guangdong Basic and Applied Basic Research Foundation,China(2023B1515120027)。
文摘CtBP-interacting protein(CtIP)is known for its multifaceted roles in DNA repair and genomic stability,directing the homologous recombination-mediated DNA double-stranded break repair pathway via DNA end resection,an essential error-free repair process vital for genome stability.Mammalian oocytes are highly prone to DNA damage accumulation due to prolonged G2/prophase arrest.Here,we explore the functions of CtIP in meiotic cell cycle regulation via a mouse oocyte model.Depletion of CtIP by siRNA injection results in delayed germinal vesicle breakdown and failed polar body extrusion.Mechanistically,CtIP deficiency increases DNA damage and decreases the expression and nuclear entry of CCNB1,resulting in marked impairment of meiotic resumption,which can be rescued by exogenous CCNB1 overexpression.Furthermore,depletion of CtIP disrupts microtubule-organizing centers coalescence at spindle poles as indicated by failed accumulation ofγ-tubulin,p-Aurora kinase A,Kif2A,and TPX2,leading to abnormal spindle assembly and prometaphase arrest.These results provide valuable insights into the important roles of CtIP in the G2/M checkpoint and spindle assembly in mouse oocyte meiotic cell cycle regulation.
基金Sincere thanks are due to the Shiraz University Research Council for his financial support.
文摘One of the most important aims of this study is to improve the core of the current VVER reactors to achieve more burn-up(or more cycle length)and more intrinsic safety.It is an independent study on the Russian new proposed FAs,called TVS-2M,which would be applied for the future advanced VVERs.Some important aspects of neutronics as well as thermal hydraulics investigations(and analysis)of the new type of Fas are conducted,and results are compared with the standards PWR CDBL.The TVS-2M FA contains gadolinium-oxide which is mixed with UO_(2)(for different Gd densities and U-235 enrichments which are given herein),but the core does not contain BARs.The new type TVS-2M Fas are modeled by the SARCS software package to find the PMAXS format for three states of CZP and HZP as well as HFP,and then the whole core is simulated by the PARCS code to investigate transient conditions.In addition,the WIMS-D5 code is suggested for steady core modeling including TVS-2M FAs and/or TVS FAs.Many neutronics aspects such as the first cycle length(first cycle burn up in terms of MWthd/kgU),the critical concentration of boric acid at the BOC as well as the cycle length,the axial,and radial power peaking factors,differential and integral worthy of the most reactive CPS-CRs,reactivity coefficients of the fuel,moderator,boric acid,and the under-moderation estimation of the core are conducted and benchmarked with the PWR CDBL.Specifically,the burn-up calculations indicate that the 45.6 d increase of the first cycle length(which corresponds to 1.18 MWthd/kgU increase of burnup)is the best improving aim of the new FA type called TVS-2M.Moreover,thermal-hydraulics core design criteria such as MDNBR(based on W3 correlation)and the maximum of fuel and clad temperatures(radially and axially),are investigated,and discussed based on the CDBL.
