AIM:To investigate the efficacy and potential mechanism of Xiaotan Tongfu granules(XTTF)in stress ulcers.METHODS:One hundred sixty rats were randomly divided into 4 groups(n=10)as follows:the model group(MP group),the...AIM:To investigate the efficacy and potential mechanism of Xiaotan Tongfu granules(XTTF)in stress ulcers.METHODS:One hundred sixty rats were randomly divided into 4 groups(n=10)as follows:the model group(MP group),the control group(CP group),the ranitidine group(RP group)and the XTTF granule group(XP group).Rats in the MP group received no drugs,rats in the CP group received 0.2 mL of a 0.9%sodium chloride solution via oral gavage,and rats in the RP and XP groups received the same volume of ranitidine(50 mg/kg)or XTTF granule(4.9 g/kg).The cold-restraint stress model was applied to induce stress ulcers after 7 consecutive days of drug administration.Afterwards,rats were sacrificed at 0,3,6 and24 h.Gastric pH was measured by a precise pH meter;gastric emptying rate(GER)was measured by using a methylcellulose test meal;myeloperoxidase activity(MPO),macrophage migration inhibitory factor(MIF),proliferating cell nuclear antigen(PCNA),and heat shock protein 70(HSP70)were measured by immunohistochemical staining;and mucosal cell apoptosis was measured by transferase dUTP nick end labeling.RESULTS:In the cold-restraint stress model,the development of stress ulcers peaked at 3 h and basically regressed after 24 h.Gastric lesions were significantly different in the RP and XP groups at each time point.Interestingly,although this index was much lower in the RP group than in the XP group immediately following stress induction(7.00±1.10 vs 10.00±1.79,P<0.05.Concerning gastric pH,between the RP and XP groups,we detected a statistically significant difference immediately after stress induction(0 h:4.56±0.47 vs 3.34±0.28,P<0.05)but not at any of the subsequent time points.For GER,compared to the RP group,GER was remarkably elevated in the XP group because a statistically significant difference was detected(3 h:46.84±2.70 vs 61.16±5.12,P<0.05;6 h:60.96±6.71 vs 73.41±6.16,P<0.05;24 h:77.47±3.17 vs 91.31±4.34,P<0.05).With respect to MPO and MIF,comparisons between the RP and XP groups revealed statistically significant differences at 3 h(MPO:18.94±1.20 vs 13.51±0.89,P<0.05;MIF:150.67±9.85 vs 122.17±5.67,P<0.05)and 6 h(MPO:13.22±1.54 vs 8.83±0.65,P<0.05;MIF:135.50±9.46 vs 109.83±6.40,P<0.05).With regard to HSP70,HSP70 expression was significantly increased in the RP and XP groups at 3 and 6 h compared to the MP and CP groups.In addition,comparing the RP and XP groups also showed statistically significant differences at 3 and 6 h.The expression of PCNA was higher in the RP and XP groups 3 h after stress induction.Between these two groups,small but statistically significant differences were observed at all of the time points(3 h:69.50±21.52 vs 79.33±15.68,P<0.05;6 h:107.83±4.40 vs 121.33±5.71,P<0.05;24 h:125.33±5.65 vs 128.50±14.49,P<0.05)except 0 h.With regard to apoptosis,the apoptotic activity in the RP and XP groups was significantly different from that in the MP and CP groups.The XP group exhibited a higher inhibition of cell apoptosis than the RP group at3 h(232.58±24.51 vs 174.46±10.35,P<0.05)and6 h(164.74±18.31 vs 117.71±12.08,P<0.05).CONCLUSION:The Xiaotan Tongfu granule was demonstrated to be similar to ranitidine in preventing stress ulcers.It exhibited multiple underlying mechanisms and deserves further study.展开更多
Objective. To observe the clinical efficacy and mechanism of Zhuyu Tongfu （逐瘀通腑, ZYTF） Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage （HCH）. Methods： One...Objective. To observe the clinical efficacy and mechanism of Zhuyu Tongfu （逐瘀通腑, ZYTF） Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage （HCH）. Methods： One hundred and eighteen patients with hypertensive cerebral hemorrhage, on the basis of conventional Western medicine treatment, were randomly divided into ZYTF combined with acupuncture and massotherapy group （treated group） and simple Western medicine group （control group） ; the clinical efficacy, neurofunction deficit scoring （NDS） alterations and hematoma absorption rate of both groups were observed, and also the plasma superoxide dismutase （SOD） activity, plasma lipid peroxidase （LPO） content, erythrocyte glutathion peroxidase （GSH-Px） activity, hematocrit （Ht） and the whole blood viscosity （Va） change were also observed. Results： In the treated group, the clinical efficacy, NDS improvement and hematoma absorption rate were superior to that of the control group; comparison between the two groups after treatment showed that plasma SOD activity and GSH-Px activity got more elevated and plasma LPO content, Ht and Va more lowered in the the treated group than those in the control group. Cenclusien： ZYTF combined with acupuncture and massotherapy has better effect, its therapeutic mechanism was possibly correlated to the elevation of plasma SOD activity, GSH-Px activity and lowering of plasma LPO content, Ht and Va.展开更多
Objective:To evaluate the efficacy and safety of XuanFei TongFu method in the treatment of sepsis.Methods:The relevant literatures on the treatment of sepsis by Xuanfei Tongfu method,published by PubMed,Medline and th...Objective:To evaluate the efficacy and safety of XuanFei TongFu method in the treatment of sepsis.Methods:The relevant literatures on the treatment of sepsis by Xuanfei Tongfu method,published by PubMed,Medline and the Cochrane library,CNKI,WEIPU Database,WANFANG Database until December 2019 were searched by computer.Revman 5.3 was used to analyze the relevant literatures that met the inclusion and exclusion criteria,and to evaluate the influence of Xuanfei Tongfu method on gastrointestinal function,adverse reactions of gastrointestinal,28 day mortality.Results:a total of 17 randomized controlled clinical trials(RCTS)involving a total of 984 patients were included.Xuanfei Tongfu decoction combined with the control group can improve intestinal function.Xuanfei Tongfu decoction can reduce gastrointestinal adverse reactions,but there is no statistical significance,and reducing mortality of 28 days(RR=0.64,95%CI(0.42,0.99),P=0.04),reducing the APACHEⅡ[SMD=-0.90,95%CI(-1.49,-0.31),P=0.0003],reducing of Il-6[SMD=-0.36,95%CI(-0.62,-0.1),P=0.007],and improving IL-10 were all better than the control group,the difference was statistically significant.Conclusion:The method of Xuanfei Tongfu can enhance the gastrointestinal function,reduce the gastrointestinal adverse reactions,and improve the prognosis of sepsis patients.It may be achieved by regulating the body's immune inflammatory response.展开更多
目的探讨通腑醒神汤联合西药治疗高血压病脑出血的临床价值。方法选取住院治疗的高血压病脑出血患者100例,随机分为对照组与观察组,每组50例。两组均给予常规西医治疗,同时观察组在对照组的治疗基础上给予通腑醒神汤辅助治疗,治疗疗程4...目的探讨通腑醒神汤联合西药治疗高血压病脑出血的临床价值。方法选取住院治疗的高血压病脑出血患者100例,随机分为对照组与观察组,每组50例。两组均给予常规西医治疗,同时观察组在对照组的治疗基础上给予通腑醒神汤辅助治疗,治疗疗程4周。观察治疗前后中医证候积分、神经功能及认知功能、血清脑损伤及自由基指标、清炎症因子水平变化。结果两组治疗前中医证候积分比较差异无统计学意义(P>0.05),治疗后积分下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05);两组患者两组治疗前格拉斯哥(Glasgow Coma Scale,GCS)、美国国立卫生研究院卒中量表(the National Institutes of Health Stroke Scale,NIHSS)、简易精神状态量表(Mini-mental State Examiniation,MMSE)、蒙特利尔认知评估量表(Montreal Cognitive Assessment,MoCA)评分比较差异无统计学意义(P>0.05),治疗后GCS、MMSE、MoCA评分升高,且观察组升高程度大于对照组,NIHSS评分下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05);两组患者两组治疗前血清神经元特异性烯醇化酶(Neuron specific enolase,NSE)、星形胶质源性蛋白(S100β)水平比较差异无统计学意义(P>0.05),治疗后NSE及S100β水平下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05);两组患者治疗前血清超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)、内皮素-1(Endothelin-1,NT-1)、一氧化氮(Nitric oxide,NO)水平比较差异无统计学意义(P>0.05),MDA、NT-1治疗后下降,且观察组下降程度大于对照组,SOD、NO治疗后升高,且观察组升高程度大于对照组,比较差异有统计学意义(P<0.05);两组患者治疗前血清白细胞介素-6(interleukin-6,IL-6)及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平比较差异无统计学意义(P>0.05),治疗后下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05)。结论通腑醒神汤联合西药治疗高血压病脑出血可明显改善患者临床症状、神经功能及认知功能,其作用可能与抑制炎性介质及自由基所致的脑损伤有关。展开更多
基金Supported by Grants from the Natural Science Foundation of ChinaNo.2010Z131the Excellent Master Training Fund of the Second Military Medical University
文摘AIM:To investigate the efficacy and potential mechanism of Xiaotan Tongfu granules(XTTF)in stress ulcers.METHODS:One hundred sixty rats were randomly divided into 4 groups(n=10)as follows:the model group(MP group),the control group(CP group),the ranitidine group(RP group)and the XTTF granule group(XP group).Rats in the MP group received no drugs,rats in the CP group received 0.2 mL of a 0.9%sodium chloride solution via oral gavage,and rats in the RP and XP groups received the same volume of ranitidine(50 mg/kg)or XTTF granule(4.9 g/kg).The cold-restraint stress model was applied to induce stress ulcers after 7 consecutive days of drug administration.Afterwards,rats were sacrificed at 0,3,6 and24 h.Gastric pH was measured by a precise pH meter;gastric emptying rate(GER)was measured by using a methylcellulose test meal;myeloperoxidase activity(MPO),macrophage migration inhibitory factor(MIF),proliferating cell nuclear antigen(PCNA),and heat shock protein 70(HSP70)were measured by immunohistochemical staining;and mucosal cell apoptosis was measured by transferase dUTP nick end labeling.RESULTS:In the cold-restraint stress model,the development of stress ulcers peaked at 3 h and basically regressed after 24 h.Gastric lesions were significantly different in the RP and XP groups at each time point.Interestingly,although this index was much lower in the RP group than in the XP group immediately following stress induction(7.00±1.10 vs 10.00±1.79,P<0.05.Concerning gastric pH,between the RP and XP groups,we detected a statistically significant difference immediately after stress induction(0 h:4.56±0.47 vs 3.34±0.28,P<0.05)but not at any of the subsequent time points.For GER,compared to the RP group,GER was remarkably elevated in the XP group because a statistically significant difference was detected(3 h:46.84±2.70 vs 61.16±5.12,P<0.05;6 h:60.96±6.71 vs 73.41±6.16,P<0.05;24 h:77.47±3.17 vs 91.31±4.34,P<0.05).With respect to MPO and MIF,comparisons between the RP and XP groups revealed statistically significant differences at 3 h(MPO:18.94±1.20 vs 13.51±0.89,P<0.05;MIF:150.67±9.85 vs 122.17±5.67,P<0.05)and 6 h(MPO:13.22±1.54 vs 8.83±0.65,P<0.05;MIF:135.50±9.46 vs 109.83±6.40,P<0.05).With regard to HSP70,HSP70 expression was significantly increased in the RP and XP groups at 3 and 6 h compared to the MP and CP groups.In addition,comparing the RP and XP groups also showed statistically significant differences at 3 and 6 h.The expression of PCNA was higher in the RP and XP groups 3 h after stress induction.Between these two groups,small but statistically significant differences were observed at all of the time points(3 h:69.50±21.52 vs 79.33±15.68,P<0.05;6 h:107.83±4.40 vs 121.33±5.71,P<0.05;24 h:125.33±5.65 vs 128.50±14.49,P<0.05)except 0 h.With regard to apoptosis,the apoptotic activity in the RP and XP groups was significantly different from that in the MP and CP groups.The XP group exhibited a higher inhibition of cell apoptosis than the RP group at3 h(232.58±24.51 vs 174.46±10.35,P<0.05)and6 h(164.74±18.31 vs 117.71±12.08,P<0.05).CONCLUSION:The Xiaotan Tongfu granule was demonstrated to be similar to ranitidine in preventing stress ulcers.It exhibited multiple underlying mechanisms and deserves further study.
文摘Objective. To observe the clinical efficacy and mechanism of Zhuyu Tongfu （逐瘀通腑, ZYTF） Serial Recipe combined with acupuncture and massotherapy in treating hypertensive cerebral hemorrhage （HCH）. Methods： One hundred and eighteen patients with hypertensive cerebral hemorrhage, on the basis of conventional Western medicine treatment, were randomly divided into ZYTF combined with acupuncture and massotherapy group （treated group） and simple Western medicine group （control group） ; the clinical efficacy, neurofunction deficit scoring （NDS） alterations and hematoma absorption rate of both groups were observed, and also the plasma superoxide dismutase （SOD） activity, plasma lipid peroxidase （LPO） content, erythrocyte glutathion peroxidase （GSH-Px） activity, hematocrit （Ht） and the whole blood viscosity （Va） change were also observed. Results： In the treated group, the clinical efficacy, NDS improvement and hematoma absorption rate were superior to that of the control group; comparison between the two groups after treatment showed that plasma SOD activity and GSH-Px activity got more elevated and plasma LPO content, Ht and Va more lowered in the the treated group than those in the control group. Cenclusien： ZYTF combined with acupuncture and massotherapy has better effect, its therapeutic mechanism was possibly correlated to the elevation of plasma SOD activity, GSH-Px activity and lowering of plasma LPO content, Ht and Va.
基金Key R&D Plan of Shaanxi Provincial Department of Science and Technology(No.2017ZDXM-SF-109)。
文摘Objective:To evaluate the efficacy and safety of XuanFei TongFu method in the treatment of sepsis.Methods:The relevant literatures on the treatment of sepsis by Xuanfei Tongfu method,published by PubMed,Medline and the Cochrane library,CNKI,WEIPU Database,WANFANG Database until December 2019 were searched by computer.Revman 5.3 was used to analyze the relevant literatures that met the inclusion and exclusion criteria,and to evaluate the influence of Xuanfei Tongfu method on gastrointestinal function,adverse reactions of gastrointestinal,28 day mortality.Results:a total of 17 randomized controlled clinical trials(RCTS)involving a total of 984 patients were included.Xuanfei Tongfu decoction combined with the control group can improve intestinal function.Xuanfei Tongfu decoction can reduce gastrointestinal adverse reactions,but there is no statistical significance,and reducing mortality of 28 days(RR=0.64,95%CI(0.42,0.99),P=0.04),reducing the APACHEⅡ[SMD=-0.90,95%CI(-1.49,-0.31),P=0.0003],reducing of Il-6[SMD=-0.36,95%CI(-0.62,-0.1),P=0.007],and improving IL-10 were all better than the control group,the difference was statistically significant.Conclusion:The method of Xuanfei Tongfu can enhance the gastrointestinal function,reduce the gastrointestinal adverse reactions,and improve the prognosis of sepsis patients.It may be achieved by regulating the body's immune inflammatory response.
文摘目的探讨通腑醒神汤联合西药治疗高血压病脑出血的临床价值。方法选取住院治疗的高血压病脑出血患者100例,随机分为对照组与观察组,每组50例。两组均给予常规西医治疗,同时观察组在对照组的治疗基础上给予通腑醒神汤辅助治疗,治疗疗程4周。观察治疗前后中医证候积分、神经功能及认知功能、血清脑损伤及自由基指标、清炎症因子水平变化。结果两组治疗前中医证候积分比较差异无统计学意义(P>0.05),治疗后积分下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05);两组患者两组治疗前格拉斯哥(Glasgow Coma Scale,GCS)、美国国立卫生研究院卒中量表(the National Institutes of Health Stroke Scale,NIHSS)、简易精神状态量表(Mini-mental State Examiniation,MMSE)、蒙特利尔认知评估量表(Montreal Cognitive Assessment,MoCA)评分比较差异无统计学意义(P>0.05),治疗后GCS、MMSE、MoCA评分升高,且观察组升高程度大于对照组,NIHSS评分下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05);两组患者两组治疗前血清神经元特异性烯醇化酶(Neuron specific enolase,NSE)、星形胶质源性蛋白(S100β)水平比较差异无统计学意义(P>0.05),治疗后NSE及S100β水平下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05);两组患者治疗前血清超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(Malondialdehyde,MDA)、内皮素-1(Endothelin-1,NT-1)、一氧化氮(Nitric oxide,NO)水平比较差异无统计学意义(P>0.05),MDA、NT-1治疗后下降,且观察组下降程度大于对照组,SOD、NO治疗后升高,且观察组升高程度大于对照组,比较差异有统计学意义(P<0.05);两组患者治疗前血清白细胞介素-6(interleukin-6,IL-6)及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平比较差异无统计学意义(P>0.05),治疗后下降,且观察组下降程度大于对照组,比较差异有统计学意义(P<0.05)。结论通腑醒神汤联合西药治疗高血压病脑出血可明显改善患者临床症状、神经功能及认知功能,其作用可能与抑制炎性介质及自由基所致的脑损伤有关。
