[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in...[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in polysaccharide from Phellinus Linteus were removed using Sevag method and TCA method.[Result] The TCA method was better than Sevag method,and the optimum protein removal condition was treated with 5% TCA for 30 min and for three times,under that condition,the protein removal rate attained 82% while the polysaccharide loss rate was only 10.8%.[Conclusion] The TCA method was a better way to remove proteins of polysaccharide from Phellinus Linteus.展开更多
The original temporal clustering analysis (OTCA) is an effective technique for obtaining brain activation maps when the timing and location of the activation are completely unknown, but its deficiency of sensitivity i...The original temporal clustering analysis (OTCA) is an effective technique for obtaining brain activation maps when the timing and location of the activation are completely unknown, but its deficiency of sensitivity is exposed in processing brain activation signal which is relatively weak. The time slice analysis method based on OTCA is proposed considering the weakness of the functional magnetic resonance imaging (fMRI) signal of the rat model. By dividing the stimulation period into several time slices and analyzing each slice to detect the activated pixels respectively after the background removal, the sensitivity is significantly improved. The inhibitory response in the hypothalamus after glucose loading is detected successfully with this method in the experiment on rat. Combined with the OTCA method, the time slice analysis method based on OTCA is effective on detecting when, where and which type of response will happen after stimulation, even if the fMRI signal is weak.展开更多
基金Supported by Youth Fund Project in Sichuan Province(2008ZQ026-072)Support Science and Technology Project of Sichuan Province(2008FZ0157)~~
文摘[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in polysaccharide from Phellinus Linteus were removed using Sevag method and TCA method.[Result] The TCA method was better than Sevag method,and the optimum protein removal condition was treated with 5% TCA for 30 min and for three times,under that condition,the protein removal rate attained 82% while the polysaccharide loss rate was only 10.8%.[Conclusion] The TCA method was a better way to remove proteins of polysaccharide from Phellinus Linteus.
基金the National Natural Science Foundation of China (30370432)
文摘The original temporal clustering analysis (OTCA) is an effective technique for obtaining brain activation maps when the timing and location of the activation are completely unknown, but its deficiency of sensitivity is exposed in processing brain activation signal which is relatively weak. The time slice analysis method based on OTCA is proposed considering the weakness of the functional magnetic resonance imaging (fMRI) signal of the rat model. By dividing the stimulation period into several time slices and analyzing each slice to detect the activated pixels respectively after the background removal, the sensitivity is significantly improved. The inhibitory response in the hypothalamus after glucose loading is detected successfully with this method in the experiment on rat. Combined with the OTCA method, the time slice analysis method based on OTCA is effective on detecting when, where and which type of response will happen after stimulation, even if the fMRI signal is weak.
