BACKGROUND The efficacy of laparoscopic surgery for the treatment of T3-T4a colon cancer remains a subject of debate in the medical community.AIM To explore the surgical techniques,perioperative outcomes,follow-up res...BACKGROUND The efficacy of laparoscopic surgery for the treatment of T3-T4a colon cancer remains a subject of debate in the medical community.AIM To explore the surgical techniques,perioperative outcomes,follow-up results,patient prognosis,and survival status associated with laparoscopic surgery for T3-T4a colon cancer.METHODS A total of 202 patients with T3-T4a colon cancer treated at the Affiliated Hospital of Hebei University between January 2020 and December 2024 were selected for this study and divided into two groups based on the type of surgery:Open surgery group(101 cases)and laparoscopic surgery group(LAP group,101 cases).Perioperative indicators(surgical time,postoperative drainage,first flatus,hospital stay,intraoperative blood loss,and number of lymph nodes removed),disease-free survival,and overall survival at 1-year and 3-year follow-ups,as well as the incidence of complications,were compared between the two groups.RESULTS The LAP group had longer surgical times and a greater number of lymph nodes removed compared to the open surgery group(P<0.05).The LAP group also had less blood loss,shorter drainage time,faster time to flatus,and a lower incidence of complications compared to the open surgery group(P<0.05).There was no significant difference in hospital stay,disease-free survival,or overall survival between the two groups during the follow-up period,and this remained true even after adjusting for subgroups based on left-sided colon,right-sided colon,and T4a stage(P>0.05).CONCLUSION The long-term outcomes of laparoscopic radical surgery for T3-T4a colon cancer are comparable to those of open surgery and can accelerate patient recovery and reduce the risk of short-term complications,offering better immediate outcomes.展开更多
The evolution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in mutations not only in the spike protein,aiding immune evasion,but also in the NSP3/4/6 proteins,crucial for regulating double...The evolution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in mutations not only in the spike protein,aiding immune evasion,but also in the NSP3/4/6 proteins,crucial for regulating double-membrane vesicle(DMV)formation.However,the functional consequences of these NSP3/4/6 mutations remain poorly understood.In this study,a systematic analysis was conducted to investigate the evolutionary patterns of NSP3/4/6 mutations and their impact on DMV formation.The findings revealed that the NSP4 T492I mutation,a prevalent mutation found in all Delta and Omicron sub-lineages,notably enhances DMV formation.Mechanistically,the NSP4 T492I mutation enhances its homodimerization,leading to an increase in the size of puncta induced by NSP3/4,and also augments endoplasmic reticulum(ER)membrane curvature,resulting in a higher DMV density per fluorescent puncta.This study underscores the significance of the NSP4 T492I mutation in modulating DMV formation,with potential implications for the transmission dynamics of SARS-CoV-2.It contributes valuable insights into how these mutations impact viral replication and pathogenesis.展开更多
基金Supported by 2024 Medical Research Program of Hebei Province,No.20240457and 2024 Medically Applicable Technology Track Project of Hebei Province,No.GZ2024099.
文摘BACKGROUND The efficacy of laparoscopic surgery for the treatment of T3-T4a colon cancer remains a subject of debate in the medical community.AIM To explore the surgical techniques,perioperative outcomes,follow-up results,patient prognosis,and survival status associated with laparoscopic surgery for T3-T4a colon cancer.METHODS A total of 202 patients with T3-T4a colon cancer treated at the Affiliated Hospital of Hebei University between January 2020 and December 2024 were selected for this study and divided into two groups based on the type of surgery:Open surgery group(101 cases)and laparoscopic surgery group(LAP group,101 cases).Perioperative indicators(surgical time,postoperative drainage,first flatus,hospital stay,intraoperative blood loss,and number of lymph nodes removed),disease-free survival,and overall survival at 1-year and 3-year follow-ups,as well as the incidence of complications,were compared between the two groups.RESULTS The LAP group had longer surgical times and a greater number of lymph nodes removed compared to the open surgery group(P<0.05).The LAP group also had less blood loss,shorter drainage time,faster time to flatus,and a lower incidence of complications compared to the open surgery group(P<0.05).There was no significant difference in hospital stay,disease-free survival,or overall survival between the two groups during the follow-up period,and this remained true even after adjusting for subgroups based on left-sided colon,right-sided colon,and T4a stage(P>0.05).CONCLUSION The long-term outcomes of laparoscopic radical surgery for T3-T4a colon cancer are comparable to those of open surgery and can accelerate patient recovery and reduce the risk of short-term complications,offering better immediate outcomes.
基金supported by the National Natural Science Foundation of China(92469107 to Z.Li)the R&D Program of Guangzhou National Laboratory(ZL-SRPG2200205 to Z.Li)the Guangdong Province High-level Talent Youth Project(2021QN02Y939 to Z.Li).
文摘The evolution of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in mutations not only in the spike protein,aiding immune evasion,but also in the NSP3/4/6 proteins,crucial for regulating double-membrane vesicle(DMV)formation.However,the functional consequences of these NSP3/4/6 mutations remain poorly understood.In this study,a systematic analysis was conducted to investigate the evolutionary patterns of NSP3/4/6 mutations and their impact on DMV formation.The findings revealed that the NSP4 T492I mutation,a prevalent mutation found in all Delta and Omicron sub-lineages,notably enhances DMV formation.Mechanistically,the NSP4 T492I mutation enhances its homodimerization,leading to an increase in the size of puncta induced by NSP3/4,and also augments endoplasmic reticulum(ER)membrane curvature,resulting in a higher DMV density per fluorescent puncta.This study underscores the significance of the NSP4 T492I mutation in modulating DMV formation,with potential implications for the transmission dynamics of SARS-CoV-2.It contributes valuable insights into how these mutations impact viral replication and pathogenesis.
