Objective To evaluate the outcomes of T3 a prostate cancer with unfavorable prognostic factors treated with permanent interstitial brachytherapy combined with external radiotherapy and hormone therapy.Methods From Jan...Objective To evaluate the outcomes of T3 a prostate cancer with unfavorable prognostic factors treated with permanent interstitial brachytherapy combined with external radiotherapy and hormone therapy.Methods From January 2003 to December 2008,38 patients classified as T3 a prostate cancer with unfavorable prognostic factors were treated with trimodality therapy(brachytherapy + external radiotherapy + hormone therapy).The prescription dose of brachytherapy and external radiotherapy were 110 Gy and 45 Gy,respectively.The duration of hormone therapy was 2-3 years.The endpoints of this study included biochemical failure-free survival(BFFS),distant metastasis-free survival(DMFS),cancer-specific survival(CSS),and overall survival(OS).Survival curves were calculated using the Kaplan-Meier method.The Log-rank test was used to identify the prognostic predictors for univariate analysis.Results The median follow-up was 71 months.The serum pre-treatment prostate-specific antigen(PSA) level ranged from 10.0 to 99.8 ng/ml(mean 56.3 ng/ml),the Gleason score ranged from 5 to 9(median 8),and the percentage of positive biopsy cores ranged from 10% to 100%(mean 65%).The 5-year BFFS,DMFS,CSS,and OS rates were 44%,69%,82%,and 76%,respectively.All biochemical failures occurred within 40 months.The percentage of positive biopsy cores was significantly correlated with BFFS,DMFS,and OS(all P=0.000),and the Gleason score with DMFS(P=0.000) and OS(P=0.001).Conclusions T3 a prostate cancer with unfavorable prognostic factors presents not so optimistic outcome.Hormone therapy should be applied to prolong the biochemical progression-free or metastasis-free survival.The percentage of positive biopsy cores and the Gleason score are significant prognostic factors.展开更多
目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微...目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。展开更多
文摘Objective To evaluate the outcomes of T3 a prostate cancer with unfavorable prognostic factors treated with permanent interstitial brachytherapy combined with external radiotherapy and hormone therapy.Methods From January 2003 to December 2008,38 patients classified as T3 a prostate cancer with unfavorable prognostic factors were treated with trimodality therapy(brachytherapy + external radiotherapy + hormone therapy).The prescription dose of brachytherapy and external radiotherapy were 110 Gy and 45 Gy,respectively.The duration of hormone therapy was 2-3 years.The endpoints of this study included biochemical failure-free survival(BFFS),distant metastasis-free survival(DMFS),cancer-specific survival(CSS),and overall survival(OS).Survival curves were calculated using the Kaplan-Meier method.The Log-rank test was used to identify the prognostic predictors for univariate analysis.Results The median follow-up was 71 months.The serum pre-treatment prostate-specific antigen(PSA) level ranged from 10.0 to 99.8 ng/ml(mean 56.3 ng/ml),the Gleason score ranged from 5 to 9(median 8),and the percentage of positive biopsy cores ranged from 10% to 100%(mean 65%).The 5-year BFFS,DMFS,CSS,and OS rates were 44%,69%,82%,and 76%,respectively.All biochemical failures occurred within 40 months.The percentage of positive biopsy cores was significantly correlated with BFFS,DMFS,and OS(all P=0.000),and the Gleason score with DMFS(P=0.000) and OS(P=0.001).Conclusions T3 a prostate cancer with unfavorable prognostic factors presents not so optimistic outcome.Hormone therapy should be applied to prolong the biochemical progression-free or metastasis-free survival.The percentage of positive biopsy cores and the Gleason score are significant prognostic factors.
文摘目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。
