摘要
目的探讨沉默T细胞免疫球蛋白黏蛋白‐3(Tim-3)基因对肿瘤相关巨噬细胞(TAMs)极化的调控作用,及这种调控对宫颈癌细胞增殖与迁移能力的影响。方法构建沉默TIM-3基因的TAMs模型,采用CCK-8实验检测宫颈癌细胞增殖能力,Transwell实验检测宫颈癌细胞迁移能力,蛋白质免疫印迹(Western blotting)实验检测上皮间质转化(EMT)相关蛋白(上皮钙黏素,神经钙黏素)的表达变化,流式细胞术检测TAMs极化状态。结果沉默TIM-3的宫颈癌细胞增殖率、迁移率显著降低,差异具有统计学意义(t=4.17~12.81,均P<0.05);E-cadherin蛋白表达量显著增高,N-cadherin蛋白表达量显著降低,差异具有统计学意义(t=8.90~25.00,均P<0.05);CD86阳性细胞比例显著升高,CD163阳性细胞比例显著降低,差异具有统计学意义(t=11.92~26.10,均P<0.05)。结论沉默TIM-3基因能够有效调控TAMs极化,显著抑制宫颈癌细胞的增殖与迁移能力,为宫颈癌的靶向治疗提供了新的理论依据和潜在靶点。
Objective This study aim to investigate the regulatory impact of the T cell immunoglobulin and mucin domain-containing protein-3(TIM-3)gene silencing on the polarization of tumor-associated macrophages(TAMs)and its influence on the proliferation and migration of cervical cancer cells.Methods The experiment constructed a TAMs model with silenced TIM-3 gene,and used CCK-8 assay to detect the proliferation ability of cervical cancer cells,Transwell assay to detect the migration ability of cervical cancer cells,Western blotting to detect the expression changes of epithelial-to-mesenchymal transition(EMT)-related proteins(E-cadherin,N-cadherin),and flow cytometry to detect the polarization state of TAMs.Results The proliferation and migration rates of cervical cancer cells with TIM-3 silencing were significantly decreased,and the differences were statistically significant(t=4.17~12.81,all P<0.05).The protein expression level of E-cadherin was significantly increased,while that of N-cadherin was significantly decreased,and the differences were statistically significant(t=8.90~25.00,all P<0.05).The proportion of CD86-positive cells increased significantly,while the proportion of CD163-positive cells decreased significantly,and the differences were statistically significant(t=11.92~26.10,all P<0.05).Conclusions The TIM-3 gene silencing regulates effectively the polarization of tumor-associated macrophages(TAMs),thereby suppressing the proliferation and migration of cervical cancer cells.This finding provides a new theoretical foundation and a promising therapeutic target for the immunotherapy of cervical cancer.
作者
邓伟伟
唐成琼
马秀敏
DENG Weiwei;TANG Chengqiong;MA Xiumin(Department of Clinical Laboratory,Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830000,China;Department of Radiation Physics and Technology,Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830000,China)
出处
《现代检验医学杂志》
2026年第1期21-23,34,共4页
Journal of Modern Laboratory Medicine
基金
新疆维吾尔自治区自然科学基金资助项目(2022D01C507)。
关键词
T细胞免疫球蛋白黏蛋白‐3
宫颈癌细胞
侵袭迁移
肿瘤相关巨噬细胞
T cell immunoglobulin and mucin domain-containing protein-3
cervical cancer cells
invasion and migration
tumor-associated macrophages
