This paper proposed a T- Y tube model to simulate foe input impedance of arterial system. It improves and extends the asymmetric T-tube model which was firstproposed by O' Rourke[1] and developed laier by Liu et a...This paper proposed a T- Y tube model to simulate foe input impedance of arterial system. It improves and extends the asymmetric T-tube model which was firstproposed by O' Rourke[1] and developed laier by Liu et al.[2]. Based on foe asymmetricT-tube model. a T-Y tube model was proposed by adding branching tubes whichrepresem the iliac arteries.All the tubes are considered to be uniform,viscoelasticlongitudinally tethered cylindrical tubes.The upper tube terminates with a windkesselmodel, while the terminal arterioles of the lowr tube are expressed as a resistance.After proper eraluation of the parameters.the impedance of the arterial system iscalculated under normal physiological and hypertensive condition.The model canpredict impedance in good agreement with the experimentally obtained data no matterin normal physiological condition or in pathological condition In comparison with theasymmeric T-tube model,T- Y tube model is closer to anatomy structure of the human arlerial system and at the sametime much simpler than the extremely complex multiplebranching tube model Therefore it will be a valuable model in studying the influencesof various parameters on aorta impedance and ventricular-vascular coupling.展开更多
目的探究Y染色体丢失(loss of Y chromosome,LOY)在膀胱癌中的作用及临床意义。方法收集2018年1月至2019年10月在广西医科大学第一附属医院接受全膀胱切除术的44例男性膀胱癌组织样本及临床资料。基于Y染色体转录特征(Y chromosome tran...目的探究Y染色体丢失(loss of Y chromosome,LOY)在膀胱癌中的作用及临床意义。方法收集2018年1月至2019年10月在广西医科大学第一附属医院接受全膀胱切除术的44例男性膀胱癌组织样本及临床资料。基于Y染色体转录特征(Y chromosome transcriptional signature,YchrS),采用单样本基因集富集分析(single-sample gene set enrichment analysis,ssGSEA)对患者的全转录组测序数据进行评分,将患者分成高YchrS评分组和低YchrS评分组。采用Kaplan-Meier法评估两组患者预后,并通过差异表达分析及基因集富集分析(gene set enrichment analysis,GSEA)揭示其潜在的分子特征。利用质谱流式细胞术(cytometry by time of flight,CyTOF)分析两组肿瘤中免疫细胞的多样性。基于公共数据库的单细胞转录组测序数据(HRA000212),通过细胞分化轨迹及细胞通讯分析LOY上皮细胞的特征。使用CMap数据库识别潜在有效治疗药物。结果低YchrS评分组膀胱癌患者表现出更短的总生存期(P=0.006)及更高的肿瘤分级(P=0.036)。该组上调基因富集于细胞周期调控、DNA复制及染色体分离等相关生物过程(P<0.05),并表现出p53抑制基因的评分升高和p53诱导基因的评分降低(均P<0.001)。相较于其他上皮细胞,LOY上皮细胞的免疫相关信号通路激活,且与髓系细胞和T细胞存在较强的相互作用。CyTOF分析显示,低YchrS评分组表现为调节性T(regulatory T,Treg)细胞富集,CD4+T细胞的CD279(PD-1)表达上调。CMap数据库分析结果显示,周期蛋白依赖性激酶(cyclin-dependent kinase,CDK)抑制剂(如哌柏西利)和组蛋白脱乙酰酶(histone deacetylase,HDAC)抑制剂是低YchrS评分组膀胱癌患者的潜在治疗药物。结论LOY与膀胱癌患者不良预后密切相关,其促癌机制可能与p53抑制及免疫抑制有关。CDK抑制剂和HDAC抑制剂是LOY膀胱癌患者潜在的治疗药物。展开更多
文摘This paper proposed a T- Y tube model to simulate foe input impedance of arterial system. It improves and extends the asymmetric T-tube model which was firstproposed by O' Rourke[1] and developed laier by Liu et al.[2]. Based on foe asymmetricT-tube model. a T-Y tube model was proposed by adding branching tubes whichrepresem the iliac arteries.All the tubes are considered to be uniform,viscoelasticlongitudinally tethered cylindrical tubes.The upper tube terminates with a windkesselmodel, while the terminal arterioles of the lowr tube are expressed as a resistance.After proper eraluation of the parameters.the impedance of the arterial system iscalculated under normal physiological and hypertensive condition.The model canpredict impedance in good agreement with the experimentally obtained data no matterin normal physiological condition or in pathological condition In comparison with theasymmeric T-tube model,T- Y tube model is closer to anatomy structure of the human arlerial system and at the sametime much simpler than the extremely complex multiplebranching tube model Therefore it will be a valuable model in studying the influencesof various parameters on aorta impedance and ventricular-vascular coupling.
文摘目的探究Y染色体丢失(loss of Y chromosome,LOY)在膀胱癌中的作用及临床意义。方法收集2018年1月至2019年10月在广西医科大学第一附属医院接受全膀胱切除术的44例男性膀胱癌组织样本及临床资料。基于Y染色体转录特征(Y chromosome transcriptional signature,YchrS),采用单样本基因集富集分析(single-sample gene set enrichment analysis,ssGSEA)对患者的全转录组测序数据进行评分,将患者分成高YchrS评分组和低YchrS评分组。采用Kaplan-Meier法评估两组患者预后,并通过差异表达分析及基因集富集分析(gene set enrichment analysis,GSEA)揭示其潜在的分子特征。利用质谱流式细胞术(cytometry by time of flight,CyTOF)分析两组肿瘤中免疫细胞的多样性。基于公共数据库的单细胞转录组测序数据(HRA000212),通过细胞分化轨迹及细胞通讯分析LOY上皮细胞的特征。使用CMap数据库识别潜在有效治疗药物。结果低YchrS评分组膀胱癌患者表现出更短的总生存期(P=0.006)及更高的肿瘤分级(P=0.036)。该组上调基因富集于细胞周期调控、DNA复制及染色体分离等相关生物过程(P<0.05),并表现出p53抑制基因的评分升高和p53诱导基因的评分降低(均P<0.001)。相较于其他上皮细胞,LOY上皮细胞的免疫相关信号通路激活,且与髓系细胞和T细胞存在较强的相互作用。CyTOF分析显示,低YchrS评分组表现为调节性T(regulatory T,Treg)细胞富集,CD4+T细胞的CD279(PD-1)表达上调。CMap数据库分析结果显示,周期蛋白依赖性激酶(cyclin-dependent kinase,CDK)抑制剂(如哌柏西利)和组蛋白脱乙酰酶(histone deacetylase,HDAC)抑制剂是低YchrS评分组膀胱癌患者的潜在治疗药物。结论LOY与膀胱癌患者不良预后密切相关,其促癌机制可能与p53抑制及免疫抑制有关。CDK抑制剂和HDAC抑制剂是LOY膀胱癌患者潜在的治疗药物。
