Objective To investigate the genetic relation am on g ten ethnic groups in northwest China. Methods Allele frequ encies of six STR(short tandem repeat ) loci in D13S1358, VWA, FGA, D5S818, D13S 317, and D7S820 were ...Objective To investigate the genetic relation am on g ten ethnic groups in northwest China. Methods Allele frequ encies of six STR(short tandem repeat ) loci in D13S1358, VWA, FGA, D5S818, D13S 317, and D7S820 were collected from Lasa Tibetan, Changdu Tibetan, Xi'an Han, G ansu Dongxiang, Gansu Yugu, Xinjiang Uygur, Ozbak, Kirgiz, Sibe, Ningxia Hui by the results of State Key laboratory, Forensic Science Department, school of medi cal, Xi'an Jiaotong University and internet biological information data bank, a nd compared with that of the Mongolian, Zhuang in China, and White and Negro in USA. The polymorphism index (H, DP, PPE, PIC) and genetic distance, then the phy logenetic tree of all population were reported. Results The res ulting tree topology exhibited strong geographic and racial partitioning consist ent with that obtained with HLA and classical genetic polymorphisms. Conclusion The results suggest that forensic STR loci may be particular ly powerful tools and provide the necessary fine resolution for the reconstructi on of recent human evolutionary history.展开更多
Objective To discuss the genetic relationship between Chaoxian and the other populations with STR polymorphisms, and study the origination of Chaoxian with historical data, analyze the difference among them. Methods S...Objective To discuss the genetic relationship between Chaoxian and the other populations with STR polymorphisms, and study the origination of Chaoxian with historical data, analyze the difference among them. Methods Samples were obtained from 91 unrelated individuals of Jilin province. PCR amplification was performed using PE9700, the results were analyzed with the GeneScan software and then using the Genetic Analyzer ABI377 and Genotyper2.5. Tests for Hardy Weinberg equilibrium (HWE) and the clustering map were carried out using SPSS11.5. The DA (genetic distance) according to Nei's was calculated and a phylogenetic tree based on the neighbor-joining method using Mega2 software package was constructed. Results 81 alleles and 196 genotypes were observed, with the corresponding frequencies ranging from 0.0055 to 0.4615 and 0.0110 to 0.2747, the observed and expected of genotypes were evaluated using χ 2-test and all of the loci were in accordance with Hardy Weinberg equilibrium (P> 0.05). Chaoxian was clustered with the other Chinese ethnic groups, then the American Black and the White; it was clustered with the Baishan Han, the Beijing Han of China first, then the South Korean. Conclusion The differentiation among races is larger than that among minorities, and, the differentiation among minorities is related to and is consistent with their geographic location.展开更多
文摘Objective To investigate the genetic relation am on g ten ethnic groups in northwest China. Methods Allele frequ encies of six STR(short tandem repeat ) loci in D13S1358, VWA, FGA, D5S818, D13S 317, and D7S820 were collected from Lasa Tibetan, Changdu Tibetan, Xi'an Han, G ansu Dongxiang, Gansu Yugu, Xinjiang Uygur, Ozbak, Kirgiz, Sibe, Ningxia Hui by the results of State Key laboratory, Forensic Science Department, school of medi cal, Xi'an Jiaotong University and internet biological information data bank, a nd compared with that of the Mongolian, Zhuang in China, and White and Negro in USA. The polymorphism index (H, DP, PPE, PIC) and genetic distance, then the phy logenetic tree of all population were reported. Results The res ulting tree topology exhibited strong geographic and racial partitioning consist ent with that obtained with HLA and classical genetic polymorphisms. Conclusion The results suggest that forensic STR loci may be particular ly powerful tools and provide the necessary fine resolution for the reconstructi on of recent human evolutionary history.
文摘Objective To discuss the genetic relationship between Chaoxian and the other populations with STR polymorphisms, and study the origination of Chaoxian with historical data, analyze the difference among them. Methods Samples were obtained from 91 unrelated individuals of Jilin province. PCR amplification was performed using PE9700, the results were analyzed with the GeneScan software and then using the Genetic Analyzer ABI377 and Genotyper2.5. Tests for Hardy Weinberg equilibrium (HWE) and the clustering map were carried out using SPSS11.5. The DA (genetic distance) according to Nei's was calculated and a phylogenetic tree based on the neighbor-joining method using Mega2 software package was constructed. Results 81 alleles and 196 genotypes were observed, with the corresponding frequencies ranging from 0.0055 to 0.4615 and 0.0110 to 0.2747, the observed and expected of genotypes were evaluated using χ 2-test and all of the loci were in accordance with Hardy Weinberg equilibrium (P> 0.05). Chaoxian was clustered with the other Chinese ethnic groups, then the American Black and the White; it was clustered with the Baishan Han, the Beijing Han of China first, then the South Korean. Conclusion The differentiation among races is larger than that among minorities, and, the differentiation among minorities is related to and is consistent with their geographic location.
