The methods of Traditional Chinese Medicine(TCM)’s diagnosis and treatment have undergone several changes.It is crucial to build a proper model which is capable to modernize TCM into a both standardized and individua...The methods of Traditional Chinese Medicine(TCM)’s diagnosis and treatment have undergone several changes.It is crucial to build a proper model which is capable to modernize TCM into a both standardized and individualized treatment.Tong xiao-lin proposed the state-target strategy to build a bridge for the integration of Chinese and Western medicine.It is a model based on modern medical disease concepts and using the method of TCM to balance the pathological states and adopting the achievements of pharmacology of Chinese medicine to focus on the disease targets,symptom targets,and biochemical indicator targets.The reconstruction of TCM diagnosis and treatment system for diabetes is a good example to demonstrate this theory.It could improve the clinical efficacy,support the scientific research,and reinforce the standardization of TCM.展开更多
Insomnia has become an urgent clinical problem in modern society.Current research has found that the gut flora-gut-brain axis plays an important role in regulating insomnia.The state-target theory is a product of the ...Insomnia has become an urgent clinical problem in modern society.Current research has found that the gut flora-gut-brain axis plays an important role in regulating insomnia.The state-target theory is a product of the combination of traditional Chinese medicine and modern molecular biology technology.This paper clarifies the correlation between the state-target theory,the intestinal flora-gut-brain axis,and liver-depression-spleen-deficiency insomnia.The use of traditional Chinese medicine to regulate the structure and abundance of intestinal flora was also explored,aiming to integrate traditional Chinese medicine with Western medicine for the prevention and treatment of liver-depression-spleen-deficiency insomnia.展开更多
目的综合分析脓毒症相关单细胞转录组学和转录组学数据集,探讨脓毒症相关态靶病理机制,构建脓毒症态靶预后模型,并挖掘潜在靶向中药及其活性成分。方法运用差异表达基因分析和韦恩图鉴定出脓毒症态靶相关基因。通过蛋白质‐蛋白相互作用...目的综合分析脓毒症相关单细胞转录组学和转录组学数据集,探讨脓毒症相关态靶病理机制,构建脓毒症态靶预后模型,并挖掘潜在靶向中药及其活性成分。方法运用差异表达基因分析和韦恩图鉴定出脓毒症态靶相关基因。通过蛋白质‐蛋白相互作用(protein‐protein interaction,PPI)网络连接度使用随机游走算法得到关键模块基因,采用基因本体论(gene ontology,GO)的生物过程(biological process,BP)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)深入分析关键模块基因的生物学功能;单细胞转录组分析探讨脓毒症态靶相关基因在其中的作用。通过脓毒症患者的基因表达数据及临床生存数据构建脓毒症态靶预后模型。采用脓毒症小鼠模型和实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-PCR)验证脓毒症态靶预后相关基因的表达水平。通过逆向网络药理学筛选脓毒症态靶相关基因的潜在靶向中药及其入血活性成分,并采用分子对接技术验证活性成分与预后相关靶点之间的结合性能。结果得到70个脓毒症毒态相关基因、67个脓毒症瘀态相关基因、54个脓毒症虚态相关基因,毒态关键模块基因与炎症密切相关,瘀态关键模块基因与凝血密切相关,虚态关键模块基因与细胞稳态密切相关。AUCell评分显示,单核细胞对脓毒症毒态和虚态作用评分最高,血小板对脓毒症瘀态作用评分最高。毒态高评分单核细胞中代谢与炎症相关基因及通路的活性明显上调;瘀态高评分血小板中血小板活化与凝血/血栓形成相关基因及通路的活性明显上调;相对于正常组,脓毒症患者中单核细胞存在大量表达下调的基因,涉及抗原呈递、细胞能量代谢、细胞周期、蛋白质合成等。基于8个靶点[白细胞介素-1受体2型(interleukin-1 receptor type 2,IL1R2)、ADP-核糖基化因子样蛋白4C(ADP ribosylation factor like GTPase 4C,ARL4C)、细胞色素C氧化酶亚基7B(cytochrome C oxidase subunit 7B,COX7B)、真核翻译起始因子2亚基γ(eukaryotic translation initiation factor 2 subunit gamma,EIF2S3)、立即早期反应3(immediate early response 3,IER3)、LSM1同源物(LSM1 homolog,LSM1)、弗林蛋白酶(paired basic amino acid cleaving enzyme,FURIN)和锚蛋白重复结构域9(ankyrin repeat domain-containing protein 9,ANKRD9)]构建的脓毒症态靶预后模型具有良好的生存预后预测能力,动物实验及RTPCR验证了其表达水平。基于中药分子机制生物信息学分析工具数据库(bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine,BATMAN-TCM)和中药血液吸收成分及代谢物数据库(database of constituents absorbed into the blood and metabolites of traditional Chinese medicine,DCABM-TCM)的逆向网络药理学分析筛选得到多种具有清热解毒、活血化瘀、补益扶正功效的中药及其入血活性成分,分子对接表明代表性入血活性成分与预后相关靶点具有良好的结合性能。结论单核细胞代谢重编程驱动下的炎症反应激活可能是脓毒症毒态的主要病理机制;血小板活化-凝血/血栓形成可能是脓毒症瘀态的主要病理机制;单核细胞功能紊乱-免疫抑制可能是脓毒症虚态的主要病理机制。基于8个基因的模型可作为脓毒症态靶预后的风险预测模型。展开更多
文摘The methods of Traditional Chinese Medicine(TCM)’s diagnosis and treatment have undergone several changes.It is crucial to build a proper model which is capable to modernize TCM into a both standardized and individualized treatment.Tong xiao-lin proposed the state-target strategy to build a bridge for the integration of Chinese and Western medicine.It is a model based on modern medical disease concepts and using the method of TCM to balance the pathological states and adopting the achievements of pharmacology of Chinese medicine to focus on the disease targets,symptom targets,and biochemical indicator targets.The reconstruction of TCM diagnosis and treatment system for diabetes is a good example to demonstrate this theory.It could improve the clinical efficacy,support the scientific research,and reinforce the standardization of TCM.
文摘Insomnia has become an urgent clinical problem in modern society.Current research has found that the gut flora-gut-brain axis plays an important role in regulating insomnia.The state-target theory is a product of the combination of traditional Chinese medicine and modern molecular biology technology.This paper clarifies the correlation between the state-target theory,the intestinal flora-gut-brain axis,and liver-depression-spleen-deficiency insomnia.The use of traditional Chinese medicine to regulate the structure and abundance of intestinal flora was also explored,aiming to integrate traditional Chinese medicine with Western medicine for the prevention and treatment of liver-depression-spleen-deficiency insomnia.
文摘目的综合分析脓毒症相关单细胞转录组学和转录组学数据集,探讨脓毒症相关态靶病理机制,构建脓毒症态靶预后模型,并挖掘潜在靶向中药及其活性成分。方法运用差异表达基因分析和韦恩图鉴定出脓毒症态靶相关基因。通过蛋白质‐蛋白相互作用(protein‐protein interaction,PPI)网络连接度使用随机游走算法得到关键模块基因,采用基因本体论(gene ontology,GO)的生物过程(biological process,BP)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)深入分析关键模块基因的生物学功能;单细胞转录组分析探讨脓毒症态靶相关基因在其中的作用。通过脓毒症患者的基因表达数据及临床生存数据构建脓毒症态靶预后模型。采用脓毒症小鼠模型和实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-PCR)验证脓毒症态靶预后相关基因的表达水平。通过逆向网络药理学筛选脓毒症态靶相关基因的潜在靶向中药及其入血活性成分,并采用分子对接技术验证活性成分与预后相关靶点之间的结合性能。结果得到70个脓毒症毒态相关基因、67个脓毒症瘀态相关基因、54个脓毒症虚态相关基因,毒态关键模块基因与炎症密切相关,瘀态关键模块基因与凝血密切相关,虚态关键模块基因与细胞稳态密切相关。AUCell评分显示,单核细胞对脓毒症毒态和虚态作用评分最高,血小板对脓毒症瘀态作用评分最高。毒态高评分单核细胞中代谢与炎症相关基因及通路的活性明显上调;瘀态高评分血小板中血小板活化与凝血/血栓形成相关基因及通路的活性明显上调;相对于正常组,脓毒症患者中单核细胞存在大量表达下调的基因,涉及抗原呈递、细胞能量代谢、细胞周期、蛋白质合成等。基于8个靶点[白细胞介素-1受体2型(interleukin-1 receptor type 2,IL1R2)、ADP-核糖基化因子样蛋白4C(ADP ribosylation factor like GTPase 4C,ARL4C)、细胞色素C氧化酶亚基7B(cytochrome C oxidase subunit 7B,COX7B)、真核翻译起始因子2亚基γ(eukaryotic translation initiation factor 2 subunit gamma,EIF2S3)、立即早期反应3(immediate early response 3,IER3)、LSM1同源物(LSM1 homolog,LSM1)、弗林蛋白酶(paired basic amino acid cleaving enzyme,FURIN)和锚蛋白重复结构域9(ankyrin repeat domain-containing protein 9,ANKRD9)]构建的脓毒症态靶预后模型具有良好的生存预后预测能力,动物实验及RTPCR验证了其表达水平。基于中药分子机制生物信息学分析工具数据库(bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine,BATMAN-TCM)和中药血液吸收成分及代谢物数据库(database of constituents absorbed into the blood and metabolites of traditional Chinese medicine,DCABM-TCM)的逆向网络药理学分析筛选得到多种具有清热解毒、活血化瘀、补益扶正功效的中药及其入血活性成分,分子对接表明代表性入血活性成分与预后相关靶点具有良好的结合性能。结论单核细胞代谢重编程驱动下的炎症反应激活可能是脓毒症毒态的主要病理机制;血小板活化-凝血/血栓形成可能是脓毒症瘀态的主要病理机制;单核细胞功能紊乱-免疫抑制可能是脓毒症虚态的主要病理机制。基于8个基因的模型可作为脓毒症态靶预后的风险预测模型。
