Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in So...Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in Soybean presents certain challenges.Therefore,we introduced the spectrum-effect relationship-ingredient knockout identification technique to identify a series of antioxidant active ingredients in soybean.By combining untargeted metabolomics with network pharmacology,we predicted the antioxidant active ingredients and their target sites.We successfully identified 4 antioxidant active compounds(daidzein,genistein,daidzein,and glycitin)and 10 corresponding antioxidant targets(epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),steroid receptor coactivator(SRC),tumor necrosis factor(TNF),kinase insert domain receptor(KDR),AKT serine/threonine kinase 1(AKT1),growth factor receptor bound protein 2(GRB2),signal transducer and activator of transcription1(STAT1),mitogen-activated protein kinase 8(MAPK8),B-cell lymphoma-2(BCL2))by our analysis.The validation results from cell experiments revealed that glycitin exhibited the best antioxidant activity and significantly influenced the expression of EGFR and the proteins associated with nuclear factor erythroid 2-related factor 2/NAD(P)H quinone dehydrogenase 1(NRF2/NQO1)signaling pathways.These findings were consistent with the predicted outcomes and were further confirmed in a zebrafish model.It suggests that glycitin may exert antioxidant effects by regulating the expression of EGFR,NRF2,and NQO1 proteins.The results demonstrate that a rapid analytical method for determining antioxidant activity was established.展开更多
Since the chromatographic fingerprint was introduced, it has been accepted by many countries to assess the quality and authenticity of Chinese herbal medicine （CHM）. However, solely using the chromatographic fingerp...Since the chromatographic fingerprint was introduced, it has been accepted by many countries to assess the quality and authenticity of Chinese herbal medicine （CHM）. However, solely using the chromatographic fingerprint to assay numerous chemicals is not suitable for the assessment of the whole internal quality and pharmacodynamics of CHM. Consequently, it is necessary to develop a rational approach to connecting the chromatographic fingerprint with effective components to assess the internal quality of CHM. For this purpose, a spec^tun-effect relationship theory was proposed and accepted as a new method for the assessment of CHM because of its potential use to screen effective components from CHM. In this paper, we systematically reviewed the application of the spectrum-effect relationship theory in the research of CHM, including research mentality, different chromatographic analysis techniques, data processing technologies, and structure determination.展开更多
Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis.The spectrum-effect relationship between chromatography fingerprints and efficacy of h...Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis.The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures.The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography(HPLC)fingerprints and anticoccidial activities.Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine.In addition,pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time.We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample.Furthermore,effect of this medicine was related with the main active constituents,along with the origin and the harvesting time.展开更多
This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network mode...This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study.展开更多
Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects o...Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF.Fingerprints of ten batches of LF were established by UPLC-PDA.The inhibitory levels of AST and ALT were used as pharmacological indexes,and secoxyloganin,isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis(GRA)and partial least squares regression analysis(PLSR).Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds.Molecular docking is used to explore the interaction between small molecules and proteins.This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF,which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food.展开更多
The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in c...The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in collaterals, which is consistent with the principle of treatment for osteoporosis. This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of gray system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin and calycosin play an extremely significant role in increasing PBM. In addition, factor analysis and hierarchical cluster analysis (HCA) are used to screen the optimal producing area for Radix Hedysari. This provides a comprehensive and efficient method to improve the quality evaluation of Radix Hedysari, confirming the bioactive components for PBM-enhancement and further develop its medicinal value.展开更多
The quantitative relationship between modern pollen and vegetation provides a critical foundation for reconstructing past vegetation,with relative pollen productivity(RPP)serving as a key calibration parameter.However...The quantitative relationship between modern pollen and vegetation provides a critical foundation for reconstructing past vegetation,with relative pollen productivity(RPP)serving as a key calibration parameter.However,in subtropical evergreen broadleaved forests(SEBFs)in China,RPP studies remain scarce,and the impact of human disturbances on RPP estimates has yet to be adequately assessed,limiting the accuracy of quantitative palaeovegetation reconstructions.This study was conducted in Dinghu Mountain Nature Reserve and its surrounding areas in Zhaoqing,Guangdong Province,and included 31 sampling sites.We performed pollen analysis alongside detailed vegetation surveys and utilized ERV submodel 2 and Prentice’s model to estimate the RPP of 9 common plant taxa in the southern SEBFs.There was a particular focus on evaluating the interference effects of bamboo plantations on the estimation of RPP.The results indicate that bamboo within the family Poaceae contributes minimally to surface soil Poaceae pollen because of its unique flowering characteristics,such as long flowering cycles and monocarpic reproduction.The incorporation of bamboo into the Poaceae vegetation coverage in the analysis led to excessively high RPP values for the other taxa.When bamboo coverage was removed from the Poaceae family,the recalculated RPP values aligned closely with those reported in previous studies.The RPP values,ranked from highest to lowest,were as follows:Castanopsis(12.33±0.03)>Araliaceae(1.60±0.03)>Mallotus(1.53±0.26)>Pinus(1.47±0.03)>Rosaceae(1.07±0.02)>Poaceae(1±0)>Euphorbiaceae(0.44±0.03)>Anacardiaceae(0.26±0.03)>Theaceae(0.15±0).Notably,the RPP values for Mallotus,Araliaceae,Theaceae,and Euphorbiaceae represent the first estimates for China’s subtropical region.Differences between certain RPP estimates and those of previous studies may be attributed to factors such as species composition,vegetation structure,and model selection.The findings of this study highlight that due to the widespread distribution of artificial bamboo forests in China’s subtropical regions,future RPP studies should carefully consider the influence of Poaceae.This consideration is essential for improving the accuracy of the application of fossil pollen for quantitative paleo-vegetation reconstruction in these regions.展开更多
Objective: This study aimed to identify the main medicinal active components of Panax ginseng(P. ginseng) in the compatibility environment of clinical application. For this purpose, the anti-inflammatory ingredients o...Objective: This study aimed to identify the main medicinal active components of Panax ginseng(P. ginseng) in the compatibility environment of clinical application. For this purpose, the anti-inflammatory ingredients of P. ginseng were investigated based on its therapeutic effect in Sijunzi Decoction(SJD) which is a widely used traditional Chinese formula.Methods: The fingerprints of 10 batches of SJD consisting of different sources of P. ginseng were established by UPLC technique to investigate the chemical components. At the same time, the antiinflammatory effects of these components were evaluated by dextran sulfate sodium-induced ulcerative colitis mouse model. Grey relational analysis was applied to explore the correlation degree between fingerprints and anti-inflammatory effects in SJD. Lipopolysaccharide-stimulated RAW264.7 murine macrophages were established to evaluate the anti-inflammatory action of the screened effective substances of P. ginseng.Results: According to grey relational analysis, notoginsenoside R1, ginsenoside Rg2 and ginsenoside Rb3of P. ginseng were the major anti-inflammatory contributions in SJD. They had been proven to be closely associated with the anti-inflammatory process of SJD and displayed a close effect compared with SJD by LPS-stimulated RAW264.7 murine macrophages.Conclusion: Our work provides a general strategy for exploring the pharmacological ingredients of P. ginseng in traditional Chinese formulas which is beneficial for establishing the quality standards of traditional herbs in traditional Chinese medicine prescription based on their clinical therapeutic effect.展开更多
Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-hi...Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-high performance liquid chromatography （UPLC） and the toxicity was evaluated by using a TAM Air Isothermal Calorimeter on Tetrahymena thermophila SB1 10. Results By analyzing the spectrum- effect relationships between UPLC fingerprints and toxic effects, the active components which had the toxic effects were obtained. Conclusion This work provides a general model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of the five Aconitum L. herbal medicines, which could be used to evaluate the toxic effects and analyze the principal toxic components of the five Aconitum L. herbal medicines. On the whole, this result provides the experimental basis for the safe use of the five Aconitum L. herbal medicines in clinic.展开更多
Lonicerae Japonicae Flos is a significant food and traditional Chinese medicine,known as plant antibiotics.It has rich chemical constituents and significant pharmacological effects.The antitumor activity of Lonicerae ...Lonicerae Japonicae Flos is a significant food and traditional Chinese medicine,known as plant antibiotics.It has rich chemical constituents and significant pharmacological effects.The antitumor activity of Lonicerae Japonicae Flos has been clarified,but the study on its spectrum-effect relationship has not been reported.The compounds responsible for its antitumor activity are still unknown.In this study,processed products of Lonicerae Japonicae Flos at different temperatures were taken as experimental materials,and SMMC-7721,A549,andMGC80-3 cells were tested.The orthogonal partial least squares regressionmethod was used to analyze the common compounds in different processed products and the antitumor activity.The results show that processed products have a stronger inhibitory effect on A549 cells and MGC80-3 cells than SMMC-7721 cells.Compounds such as secologanic acid,isochlorogenic acid A,serotonin,and chlorogenic acid play an important role in their antitumor effects.展开更多
In the international shipping industry, digital intelligence transformation has become essential, with both governments and enterprises actively working to integrate diverse datasets. The domain of maritime and shippi...In the international shipping industry, digital intelligence transformation has become essential, with both governments and enterprises actively working to integrate diverse datasets. The domain of maritime and shipping is characterized by a vast array of document types, filled with complex, large-scale, and often chaotic knowledge and relationships. Effectively managing these documents is crucial for developing a Large Language Model (LLM) in the maritime domain, enabling practitioners to access and leverage valuable information. A Knowledge Graph (KG) offers a state-of-the-art solution for enhancing knowledge retrieval, providing more accurate responses and enabling context-aware reasoning. This paper presents a framework for utilizing maritime and shipping documents to construct a knowledge graph using GraphRAG, a hybrid tool combining graph-based retrieval and generation capabilities. The extraction of entities and relationships from these documents and the KG construction process are detailed. Furthermore, the KG is integrated with an LLM to develop a Q&A system, demonstrating that the system significantly improves answer accuracy compared to traditional LLMs. Additionally, the KG construction process is up to 50% faster than conventional LLM-based approaches, underscoring the efficiency of our method. This study provides a promising approach to digital intelligence in shipping, advancing knowledge accessibility and decision-making.展开更多
In this editorial,I comment on the article by Zhang et al.To emphasize the importance of the topic,I discuss the relationship between the use of smart medical devices and mental health.Smart medical services have the ...In this editorial,I comment on the article by Zhang et al.To emphasize the importance of the topic,I discuss the relationship between the use of smart medical devices and mental health.Smart medical services have the potential to positively influence mental health by providing monitoring,insights,and inter-ventions.However,they also come with challenges that need to be addressed.Understanding the primary purpose for which individuals use these smart tech-nologies is essential to tailoring them to specific mental health needs and prefe-rences.展开更多
This study investigated the role of intentional self-regulation and the moderating role of peer relationship in the relationship between teacher-student relationship and learning engagement.The study sample comprised ...This study investigated the role of intentional self-regulation and the moderating role of peer relationship in the relationship between teacher-student relationship and learning engagement.The study sample comprised 540 Chinese senior secondary school students between the ages of 15–18(51.67%boys;Mage=16.56 years;SDage=0.90).They completed surveys on the Teacher-Student Relationship Scale,the Selection,Optimization,and Compensation(SOC)Scale,the Peer Relationship Scale for Children and Adolescents,and the Learning Engagement Scale.The results following regression analysis showed that teacher-student relationship predicted higher learning engagement among senior secondary school students.Intentional self-regulation partially mediated the link between teacher-student relationship and learning engagement for higher learning engagement.Peer relationship moderated the relationships between teacher-student relationship and learning engagement and moderated the relationship between teacher-student relationship and intentional self-regulation for higher learning engagement.Thesefindings imply learning engagement can be enhanced by optimizing teacher-student relationship and strengthening intentional self-regulation interventions.展开更多
The relationship between ecosystem services(ES)and human well-being(HWB)is fundamental to the science and practice of sustainability.However,studies have shown conflicting results,which has been attributed to the infl...The relationship between ecosystem services(ES)and human well-being(HWB)is fundamental to the science and practice of sustainability.However,studies have shown conflicting results,which has been attributed to the influences of indicators,contexts,and scales.Yet,another potential factor,which has been overlooked,may be the mixed use of spatial and temporal approaches.Using twelve ES and seven well-being indicators and multiple statistical methods,we quantified and compared the spatial and temporal ES–HWB relationships for Inner Mongolia,China.The spatial and temporal relationships differed in both correlation direction and strength.Most relationships of economic and employment-related indicators with food provisioning and supporting services were temporally positive but spatially nonsignificant or negative.Some relationships of economic and employmentrelated indicators with water retention,sandstorm prevention,and wind erosion were temporally negative but spatially complex.However,the spatial and temporal ES–HWB relationships could also be similar in some cases.We conclude that although both the spatial and temporal approaches have merits,space generally cannot substitute for time in the study of ES–HWB relationship.Our study helps reconcile the seemingly conflicting findings in the literature,and suggests that future studies should explicitly distinguish between the spatial and temporal ES–HWB relationships.展开更多
This study examined the impact of teacher-student relationship quality on students’risk of bullying victimiza-tion and the mediating roles of student-student relationships and student engagement in this relationship....This study examined the impact of teacher-student relationship quality on students’risk of bullying victimiza-tion and the mediating roles of student-student relationships and student engagement in this relationship.A total of 656 Chinese junior high school students(females=361,mean age=13.75,SD=0.98)completed validated measures of teacher-student relationship quality,student-student relationship quality,student engagement,and bullying victimization.Regression analysis results indicated that higher teacher-student relationship quality predicted a lower risk of student bullying victimization.Serial mediating effect testing of the student-student relationship quality and student engagement revealed that these factors fully mediated the relationship between teacher-student relationship quality and bullying victimization,resulting in a lower risk of bullying victimization.The results showed that student-student relationship quality had a more substantial mediating effect than student engagement.Thefindings support the Socio-Ecological Framework,suggesting that within the Microsystem,interactions between individuals and their immediate environments significantly impact their behavior.Specifically,thesefindings suggest that good teacher-student relationships can enhance the quality of student-student relationships and student engagement,thereby preventing and reducing the occurrence of bullying victimization.展开更多
Since China’s reform and development,under the influence of the global religious and cultural boom,it has also focused on studying Islamic culture and related fields,resulting in many important representative works.T...Since China’s reform and development,under the influence of the global religious and cultural boom,it has also focused on studying Islamic culture and related fields,resulting in many important representative works.The main characteristic of writing books in this area is to use popular knowledge materials that are suitable for various sectors of society.There are also works exploring the historical evolution of Islamic culture in China,which involve the relationship between Islamic culture and traditional Chinese culture.Before the 1980s,there were no significant achievements in publishing papers.However,since the 1990s,there has been a noticeable increase in papers related to Islamic culture and related fields,with new advances in research areas and methods.There are both articles that explore the attributes and characteristics of Islamic culture from the perspectives of world history and culture,as well as many articles that explore various specific aspects of Islamic culture from the perspective of specific topics.In this article,the author collects academic achievements in the study of Islamic culture in China and introduces them to the academic community,letting them know about China's research on world Islamic culture.展开更多
BACKGROUND Hyperglycemia secondary to diabetes can lead to organ failure unless properly controlled,and it is associated with numerous health issues,particularly metabolic problems.Breast cancer is one of the most com...BACKGROUND Hyperglycemia secondary to diabetes can lead to organ failure unless properly controlled,and it is associated with numerous health issues,particularly metabolic problems.Breast cancer is one of the most common malignancies in females worldwide and represents a major health concern due to its morbidity and mortality.The progression of the disease and the response to treatment depend on numerous variables,including both tumor biology and patient-related factors.Key prognostic indicators include age,tumor size,lymph node involvement,histological type,hormone receptor status,and expression of biomarkers such as HER2.AIM To investigate the relationship between preoperative blood glucose levels and cancer prognostic factors in patients diagnosed with breast cancer.METHODS In this study patients diagnosed with breast cancer were classified into two groups:Those with blood glucose levels<125 mg/dL and those with levels>125 mg/dL.Estrogen and progesterone receptor positivity,Ki-67 percentage,Cerb2 positivity,number of positive lymph nodes,and presence of lymphovascular invasion were examined histopathologically.RESULTS The study included 246 patients with breast cancer:196 were in the normal glucose group and 50 were in the elevated glucose group.In the normal glucose group,68%of the tumors were left-sided and 32%were right-sided,while all tumors in the elevated glucose group were left-sided.This difference was statistically significant(P<0.001).Tumor size,Ki-67,and the number of positive lymph nodes were not associated with blood glucose in patients with breast cancer(P>0.05 for all).However,blood glucose was correlated with age in this population.Correlation analyses revealed that blood glucose was positively correlated with age(r=0.23,P<0.001).Additionally,Ki-67 was correlated with tumor size(r=0.14,P=0.03)and age(r=-0.18,P=0.005).CONCLUSION Our study highlighted the complex interplay between metabolic factors,such as fasting glucose levels,and breast cancer characteristics.Elevated glucose levels were associated with older age.The significant association between tumor laterality and glucose levels underscores the need for further investigation into the collective influence of metabolic and anatomical factors on breast cancer development and progression.展开更多
Background With the rapid growth of internet usage,adolescent cyberbullying has become a pressing issue.This study examines the longitudinal impact of leisure-time physical activity and peer relationships on cyberbull...Background With the rapid growth of internet usage,adolescent cyberbullying has become a pressing issue.This study examines the longitudinal impact of leisure-time physical activity and peer relationships on cyberbullying over a one-year period,drawing on the Stage-Environment Fit Theory and the Interpersonal Relationship Theory.Methods A three-wave longitudinal study was conducted over one year,involving 896 middle school students from five schools in Sichuan,Jiangsu,and Guangdong,China,selected to ensure regional diversity.Participants were recruited using stratified random sampling,and data were collected at four-month intervals.Leisure-time physical activity,peer relationships,and cyberbullying behaviors were assessed using validated scales.Data were analyzed using latent growth modeling(LGM)and structural equation modeling(SEM)to examine longitudinal effects and mediation relationships.Results(1)Leisure-time physical activity,peer relationships,and cyberbullying behaviors remained relatively stable over time.(2)Both leisure-time physical activity and peer relationships significantly reduced future cyberbullying incidents,with physical activity also enhancing subsequent peer relationships.(3)Peer relationships partially mediated the effect of leisure-time physical activity on cyberbullying,indicating that improved peer interactions contributed to a reduction in cyberbullying behaviors.Conclusion This study found that leisure-time sports activities and peer relationships are important factors affecting cyberbullying,and peer relationships play a partial mediating role in it.The results provide empirical support for understanding the formation mechanism and influencing factors of cyberbullying.展开更多
基金supported by National Key R&D Program of China(2022YFF1100300)Joint Fund of Henan Province Science and Technology R&D Program(235200810051)+1 种基金Key Project in Science and Technology Agency of Henan Province(242102310561)key research projects of higher education institutions in Henan Province(24B350002).
文摘Soybean is widely used in diets,and numerous reports have highlighted its antioxidant properties.However,constructing a methodology for rapid identifying and predicting a series of antioxidant active ingredients in Soybean presents certain challenges.Therefore,we introduced the spectrum-effect relationship-ingredient knockout identification technique to identify a series of antioxidant active ingredients in soybean.By combining untargeted metabolomics with network pharmacology,we predicted the antioxidant active ingredients and their target sites.We successfully identified 4 antioxidant active compounds(daidzein,genistein,daidzein,and glycitin)and 10 corresponding antioxidant targets(epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),steroid receptor coactivator(SRC),tumor necrosis factor(TNF),kinase insert domain receptor(KDR),AKT serine/threonine kinase 1(AKT1),growth factor receptor bound protein 2(GRB2),signal transducer and activator of transcription1(STAT1),mitogen-activated protein kinase 8(MAPK8),B-cell lymphoma-2(BCL2))by our analysis.The validation results from cell experiments revealed that glycitin exhibited the best antioxidant activity and significantly influenced the expression of EGFR and the proteins associated with nuclear factor erythroid 2-related factor 2/NAD(P)H quinone dehydrogenase 1(NRF2/NQO1)signaling pathways.These findings were consistent with the predicted outcomes and were further confirmed in a zebrafish model.It suggests that glycitin may exert antioxidant effects by regulating the expression of EGFR,NRF2,and NQO1 proteins.The results demonstrate that a rapid analytical method for determining antioxidant activity was established.
基金supported by Ministry of Education Research Fund for the Doctoral Program(20130013120001 and 20120013130002)Beijing University of Chinese Medicine Young Teachers of Special Autonomy Issue(2013-QNJSZX008)Beijing University of Chinese Medicine Scientific Research Innovation Team(2011-CXTD-014)
文摘Since the chromatographic fingerprint was introduced, it has been accepted by many countries to assess the quality and authenticity of Chinese herbal medicine （CHM）. However, solely using the chromatographic fingerprint to assay numerous chemicals is not suitable for the assessment of the whole internal quality and pharmacodynamics of CHM. Consequently, it is necessary to develop a rational approach to connecting the chromatographic fingerprint with effective components to assess the internal quality of CHM. For this purpose, a spec^tun-effect relationship theory was proposed and accepted as a new method for the assessment of CHM because of its potential use to screen effective components from CHM. In this paper, we systematically reviewed the application of the spectrum-effect relationship theory in the research of CHM, including research mentality, different chromatographic analysis techniques, data processing technologies, and structure determination.
基金funded by the National Key Technology R&D Program,China(2008 BADB4B05-02)
文摘Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis.The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures.The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography(HPLC)fingerprints and anticoccidial activities.Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine.In addition,pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time.We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample.Furthermore,effect of this medicine was related with the main active constituents,along with the origin and the harvesting time.
基金This work was funded by the National Key R&D Program of China(2018YFD0400200)Key Project in Science and Technology Agency of Kaifeng City(1906006)Major Public Welfare Projects in Henan Province(201300110200).
文摘This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study.
基金financially supported by the National Natural Science Foundation of China (81973604, 81803690 and 81703684)Special Funds from the Central Finance to Support the Development of Local Universities+12 种基金the National Natural Science Foundation Matching Project (2018PT02)the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (2018RCD25)the Postdoctoral Initial Fund of Heilongjiang Province (UNPYSCT 2017219)the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2017215)the National Natural Science Foundation Matching Project (2017PT01)the Natural Science Foundation of Heilongjiang Province (H2015037)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (2014bs05)the Application Technology Research and Development Projects of Harbin Technology Bureau (2014RFQXJ149)the Heilongjiang Postdoctoral Scientific Research Developmental Fund (LBH-Q16210 and LBH-Q17161)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (2013bs04)the scientific research project of Heilongjiang Provincial Health Commission (20211313050171)Heilongjiang Touyan Innovation Team ProgramNational Famous Old Traditional Chinese Medecine Experts Inheritance Studio Construction Program of National Administration of TCM ([2022]No.75)
文摘Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF.Fingerprints of ten batches of LF were established by UPLC-PDA.The inhibitory levels of AST and ALT were used as pharmacological indexes,and secoxyloganin,isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis(GRA)and partial least squares regression analysis(PLSR).Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds.Molecular docking is used to explore the interaction between small molecules and proteins.This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF,which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food.
基金supported by the National Natural Science Funds of China(Grant No.81703664)Science and Technology Funds of Lanzhou,China(Grant No.201603111)
文摘The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in collaterals, which is consistent with the principle of treatment for osteoporosis. This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of gray system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin and calycosin play an extremely significant role in increasing PBM. In addition, factor analysis and hierarchical cluster analysis (HCA) are used to screen the optimal producing area for Radix Hedysari. This provides a comprehensive and efficient method to improve the quality evaluation of Radix Hedysari, confirming the bioactive components for PBM-enhancement and further develop its medicinal value.
基金supported by the National Natural Science Foundation of China(Grant Nos.42407595&41630753)the National Key Research and Development Program of China(Grant No.2022YFF0801501).
文摘The quantitative relationship between modern pollen and vegetation provides a critical foundation for reconstructing past vegetation,with relative pollen productivity(RPP)serving as a key calibration parameter.However,in subtropical evergreen broadleaved forests(SEBFs)in China,RPP studies remain scarce,and the impact of human disturbances on RPP estimates has yet to be adequately assessed,limiting the accuracy of quantitative palaeovegetation reconstructions.This study was conducted in Dinghu Mountain Nature Reserve and its surrounding areas in Zhaoqing,Guangdong Province,and included 31 sampling sites.We performed pollen analysis alongside detailed vegetation surveys and utilized ERV submodel 2 and Prentice’s model to estimate the RPP of 9 common plant taxa in the southern SEBFs.There was a particular focus on evaluating the interference effects of bamboo plantations on the estimation of RPP.The results indicate that bamboo within the family Poaceae contributes minimally to surface soil Poaceae pollen because of its unique flowering characteristics,such as long flowering cycles and monocarpic reproduction.The incorporation of bamboo into the Poaceae vegetation coverage in the analysis led to excessively high RPP values for the other taxa.When bamboo coverage was removed from the Poaceae family,the recalculated RPP values aligned closely with those reported in previous studies.The RPP values,ranked from highest to lowest,were as follows:Castanopsis(12.33±0.03)>Araliaceae(1.60±0.03)>Mallotus(1.53±0.26)>Pinus(1.47±0.03)>Rosaceae(1.07±0.02)>Poaceae(1±0)>Euphorbiaceae(0.44±0.03)>Anacardiaceae(0.26±0.03)>Theaceae(0.15±0).Notably,the RPP values for Mallotus,Araliaceae,Theaceae,and Euphorbiaceae represent the first estimates for China’s subtropical region.Differences between certain RPP estimates and those of previous studies may be attributed to factors such as species composition,vegetation structure,and model selection.The findings of this study highlight that due to the widespread distribution of artificial bamboo forests in China’s subtropical regions,future RPP studies should carefully consider the influence of Poaceae.This consideration is essential for improving the accuracy of the application of fossil pollen for quantitative paleo-vegetation reconstruction in these regions.
基金financially supported by Science Foundation of Jilin Educational Committee (No. JJKH20200363KJ)Jilin Science & Technology Development Plan (No. 20190304009YY). Jilin Science & Technology Development Plan (No. 20200404090YY)。
文摘Objective: This study aimed to identify the main medicinal active components of Panax ginseng(P. ginseng) in the compatibility environment of clinical application. For this purpose, the anti-inflammatory ingredients of P. ginseng were investigated based on its therapeutic effect in Sijunzi Decoction(SJD) which is a widely used traditional Chinese formula.Methods: The fingerprints of 10 batches of SJD consisting of different sources of P. ginseng were established by UPLC technique to investigate the chemical components. At the same time, the antiinflammatory effects of these components were evaluated by dextran sulfate sodium-induced ulcerative colitis mouse model. Grey relational analysis was applied to explore the correlation degree between fingerprints and anti-inflammatory effects in SJD. Lipopolysaccharide-stimulated RAW264.7 murine macrophages were established to evaluate the anti-inflammatory action of the screened effective substances of P. ginseng.Results: According to grey relational analysis, notoginsenoside R1, ginsenoside Rg2 and ginsenoside Rb3of P. ginseng were the major anti-inflammatory contributions in SJD. They had been proven to be closely associated with the anti-inflammatory process of SJD and displayed a close effect compared with SJD by LPS-stimulated RAW264.7 murine macrophages.Conclusion: Our work provides a general strategy for exploring the pharmacological ingredients of P. ginseng in traditional Chinese formulas which is beneficial for establishing the quality standards of traditional herbs in traditional Chinese medicine prescription based on their clinical therapeutic effect.
基金State Natural Science Foundation(81173571)The"Twelfth Five-Year Plan"Foundation of China People’s Liberation Army(CWS11C164)The Major Projects of the National Science and Technology(2012ZX10005010-002-002)
文摘Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-high performance liquid chromatography （UPLC） and the toxicity was evaluated by using a TAM Air Isothermal Calorimeter on Tetrahymena thermophila SB1 10. Results By analyzing the spectrum- effect relationships between UPLC fingerprints and toxic effects, the active components which had the toxic effects were obtained. Conclusion This work provides a general model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of the five Aconitum L. herbal medicines, which could be used to evaluate the toxic effects and analyze the principal toxic components of the five Aconitum L. herbal medicines. On the whole, this result provides the experimental basis for the safe use of the five Aconitum L. herbal medicines in clinic.
基金supported by the Scientific and Technological Research Project of Henan Province(grant no.242102310549)the Key Research and Development Programme of Henan Province(grant no.231111312700)+2 种基金the National Natural Science Foundation of China(grant no.82104329)theNational Key Research andDevelopment Programme of China(grant no.2017YFC1702800)the special funds for starting scientific research of Henan University of Chinese Medicine(grant no.00104311-2021-1-41).
文摘Lonicerae Japonicae Flos is a significant food and traditional Chinese medicine,known as plant antibiotics.It has rich chemical constituents and significant pharmacological effects.The antitumor activity of Lonicerae Japonicae Flos has been clarified,but the study on its spectrum-effect relationship has not been reported.The compounds responsible for its antitumor activity are still unknown.In this study,processed products of Lonicerae Japonicae Flos at different temperatures were taken as experimental materials,and SMMC-7721,A549,andMGC80-3 cells were tested.The orthogonal partial least squares regressionmethod was used to analyze the common compounds in different processed products and the antitumor activity.The results show that processed products have a stronger inhibitory effect on A549 cells and MGC80-3 cells than SMMC-7721 cells.Compounds such as secologanic acid,isochlorogenic acid A,serotonin,and chlorogenic acid play an important role in their antitumor effects.
文摘In the international shipping industry, digital intelligence transformation has become essential, with both governments and enterprises actively working to integrate diverse datasets. The domain of maritime and shipping is characterized by a vast array of document types, filled with complex, large-scale, and often chaotic knowledge and relationships. Effectively managing these documents is crucial for developing a Large Language Model (LLM) in the maritime domain, enabling practitioners to access and leverage valuable information. A Knowledge Graph (KG) offers a state-of-the-art solution for enhancing knowledge retrieval, providing more accurate responses and enabling context-aware reasoning. This paper presents a framework for utilizing maritime and shipping documents to construct a knowledge graph using GraphRAG, a hybrid tool combining graph-based retrieval and generation capabilities. The extraction of entities and relationships from these documents and the KG construction process are detailed. Furthermore, the KG is integrated with an LLM to develop a Q&A system, demonstrating that the system significantly improves answer accuracy compared to traditional LLMs. Additionally, the KG construction process is up to 50% faster than conventional LLM-based approaches, underscoring the efficiency of our method. This study provides a promising approach to digital intelligence in shipping, advancing knowledge accessibility and decision-making.
文摘In this editorial,I comment on the article by Zhang et al.To emphasize the importance of the topic,I discuss the relationship between the use of smart medical devices and mental health.Smart medical services have the potential to positively influence mental health by providing monitoring,insights,and inter-ventions.However,they also come with challenges that need to be addressed.Understanding the primary purpose for which individuals use these smart tech-nologies is essential to tailoring them to specific mental health needs and prefe-rences.
文摘This study investigated the role of intentional self-regulation and the moderating role of peer relationship in the relationship between teacher-student relationship and learning engagement.The study sample comprised 540 Chinese senior secondary school students between the ages of 15–18(51.67%boys;Mage=16.56 years;SDage=0.90).They completed surveys on the Teacher-Student Relationship Scale,the Selection,Optimization,and Compensation(SOC)Scale,the Peer Relationship Scale for Children and Adolescents,and the Learning Engagement Scale.The results following regression analysis showed that teacher-student relationship predicted higher learning engagement among senior secondary school students.Intentional self-regulation partially mediated the link between teacher-student relationship and learning engagement for higher learning engagement.Peer relationship moderated the relationships between teacher-student relationship and learning engagement and moderated the relationship between teacher-student relationship and intentional self-regulation for higher learning engagement.Thesefindings imply learning engagement can be enhanced by optimizing teacher-student relationship and strengthening intentional self-regulation interventions.
基金supported by the Fundamental Research Funds for the Central Universities(Grant No.B240201068)the National Natural Science Foundation of China(Grant No.42361144861)the National Basic Research Program of China(Grant No.2014CB954303).
文摘The relationship between ecosystem services(ES)and human well-being(HWB)is fundamental to the science and practice of sustainability.However,studies have shown conflicting results,which has been attributed to the influences of indicators,contexts,and scales.Yet,another potential factor,which has been overlooked,may be the mixed use of spatial and temporal approaches.Using twelve ES and seven well-being indicators and multiple statistical methods,we quantified and compared the spatial and temporal ES–HWB relationships for Inner Mongolia,China.The spatial and temporal relationships differed in both correlation direction and strength.Most relationships of economic and employment-related indicators with food provisioning and supporting services were temporally positive but spatially nonsignificant or negative.Some relationships of economic and employmentrelated indicators with water retention,sandstorm prevention,and wind erosion were temporally negative but spatially complex.However,the spatial and temporal ES–HWB relationships could also be similar in some cases.We conclude that although both the spatial and temporal approaches have merits,space generally cannot substitute for time in the study of ES–HWB relationship.Our study helps reconcile the seemingly conflicting findings in the literature,and suggests that future studies should explicitly distinguish between the spatial and temporal ES–HWB relationships.
基金supported by the 2024 Henan Province Philosophy and Social Science Planning Project(Youth Project)entitled“Research on the Mechanism and Intervention of Self-Regulated Learning in Promoting Children’s Chinese Reading Comprehension”(2024CJY070).
文摘This study examined the impact of teacher-student relationship quality on students’risk of bullying victimiza-tion and the mediating roles of student-student relationships and student engagement in this relationship.A total of 656 Chinese junior high school students(females=361,mean age=13.75,SD=0.98)completed validated measures of teacher-student relationship quality,student-student relationship quality,student engagement,and bullying victimization.Regression analysis results indicated that higher teacher-student relationship quality predicted a lower risk of student bullying victimization.Serial mediating effect testing of the student-student relationship quality and student engagement revealed that these factors fully mediated the relationship between teacher-student relationship quality and bullying victimization,resulting in a lower risk of bullying victimization.The results showed that student-student relationship quality had a more substantial mediating effect than student engagement.Thefindings support the Socio-Ecological Framework,suggesting that within the Microsystem,interactions between individuals and their immediate environments significantly impact their behavior.Specifically,thesefindings suggest that good teacher-student relationships can enhance the quality of student-student relationships and student engagement,thereby preventing and reducing the occurrence of bullying victimization.
文摘Since China’s reform and development,under the influence of the global religious and cultural boom,it has also focused on studying Islamic culture and related fields,resulting in many important representative works.The main characteristic of writing books in this area is to use popular knowledge materials that are suitable for various sectors of society.There are also works exploring the historical evolution of Islamic culture in China,which involve the relationship between Islamic culture and traditional Chinese culture.Before the 1980s,there were no significant achievements in publishing papers.However,since the 1990s,there has been a noticeable increase in papers related to Islamic culture and related fields,with new advances in research areas and methods.There are both articles that explore the attributes and characteristics of Islamic culture from the perspectives of world history and culture,as well as many articles that explore various specific aspects of Islamic culture from the perspective of specific topics.In this article,the author collects academic achievements in the study of Islamic culture in China and introduces them to the academic community,letting them know about China's research on world Islamic culture.
文摘BACKGROUND Hyperglycemia secondary to diabetes can lead to organ failure unless properly controlled,and it is associated with numerous health issues,particularly metabolic problems.Breast cancer is one of the most common malignancies in females worldwide and represents a major health concern due to its morbidity and mortality.The progression of the disease and the response to treatment depend on numerous variables,including both tumor biology and patient-related factors.Key prognostic indicators include age,tumor size,lymph node involvement,histological type,hormone receptor status,and expression of biomarkers such as HER2.AIM To investigate the relationship between preoperative blood glucose levels and cancer prognostic factors in patients diagnosed with breast cancer.METHODS In this study patients diagnosed with breast cancer were classified into two groups:Those with blood glucose levels<125 mg/dL and those with levels>125 mg/dL.Estrogen and progesterone receptor positivity,Ki-67 percentage,Cerb2 positivity,number of positive lymph nodes,and presence of lymphovascular invasion were examined histopathologically.RESULTS The study included 246 patients with breast cancer:196 were in the normal glucose group and 50 were in the elevated glucose group.In the normal glucose group,68%of the tumors were left-sided and 32%were right-sided,while all tumors in the elevated glucose group were left-sided.This difference was statistically significant(P<0.001).Tumor size,Ki-67,and the number of positive lymph nodes were not associated with blood glucose in patients with breast cancer(P>0.05 for all).However,blood glucose was correlated with age in this population.Correlation analyses revealed that blood glucose was positively correlated with age(r=0.23,P<0.001).Additionally,Ki-67 was correlated with tumor size(r=0.14,P=0.03)and age(r=-0.18,P=0.005).CONCLUSION Our study highlighted the complex interplay between metabolic factors,such as fasting glucose levels,and breast cancer characteristics.Elevated glucose levels were associated with older age.The significant association between tumor laterality and glucose levels underscores the need for further investigation into the collective influence of metabolic and anatomical factors on breast cancer development and progression.
基金funded by the following projects:National Social Science Fund Project“The Empowerment Mechanism of Physical Exercise on Emotional Regulation in Adolescents”(23BTY116)Leshan Normal University 2024 Research Cultivation Project:“Research on the Trajectory Effect of Family Cumulative Risk and Home-based Activity of Adolescents”(KYPY2025-0014)+1 种基金Key Humanities and Social Sciences Cultivation Project of Leshan Normal University:“Research on the Sequence Difference of Knowledge and Behavior of Physical Activity among Adolescents and the Compensation Mechanism”and Sichuan Province College Students’Sports Association Annual Project“The Trajectory Effect of Family Cumulative Risk and Adolescents’Home Physical Activity”(23CDTXQ004).
文摘Background With the rapid growth of internet usage,adolescent cyberbullying has become a pressing issue.This study examines the longitudinal impact of leisure-time physical activity and peer relationships on cyberbullying over a one-year period,drawing on the Stage-Environment Fit Theory and the Interpersonal Relationship Theory.Methods A three-wave longitudinal study was conducted over one year,involving 896 middle school students from five schools in Sichuan,Jiangsu,and Guangdong,China,selected to ensure regional diversity.Participants were recruited using stratified random sampling,and data were collected at four-month intervals.Leisure-time physical activity,peer relationships,and cyberbullying behaviors were assessed using validated scales.Data were analyzed using latent growth modeling(LGM)and structural equation modeling(SEM)to examine longitudinal effects and mediation relationships.Results(1)Leisure-time physical activity,peer relationships,and cyberbullying behaviors remained relatively stable over time.(2)Both leisure-time physical activity and peer relationships significantly reduced future cyberbullying incidents,with physical activity also enhancing subsequent peer relationships.(3)Peer relationships partially mediated the effect of leisure-time physical activity on cyberbullying,indicating that improved peer interactions contributed to a reduction in cyberbullying behaviors.Conclusion This study found that leisure-time sports activities and peer relationships are important factors affecting cyberbullying,and peer relationships play a partial mediating role in it.The results provide empirical support for understanding the formation mechanism and influencing factors of cyberbullying.
