Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin.Method : We used rapid electrophoretic method on cellulose acetate plate to ...Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin.Method : We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (aa,aγ and γγ),and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of fluorometer.Result: Aqueous from all patients with Rb contained aa,ar and rr isoenzymes and presented strong postitive, the positive rate of NSE being 100% and its relative activity accounting for 45 ± 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats's diseases (4 cases),but in two展开更多
目的 探讨手足口病(hand-foot-mouth disease,HFMD)患儿中血清淀粉样蛋白A/C反应蛋白(serum amyloid A/C reaction protein,SAA/CRP)、磷酸化信号转导和转录激活因子3(phosphorylated signal transducer and activator of transcription...目的 探讨手足口病(hand-foot-mouth disease,HFMD)患儿中血清淀粉样蛋白A/C反应蛋白(serum amyloid A/C reaction protein,SAA/CRP)、磷酸化信号转导和转录激活因子3(phosphorylated signal transducer and activator of transcription 3,p-STAT3)、神经元特异性烯醇化酶(neuron specific enolase,NSE)水平变化,为评估病情严重程度及预测价值提供科学依据。方法 将2022年3月至2024年3月收治的104例HFMD患儿分为轻症组、重症组、危重症组。选取同期入院进行健康体检的52例儿童作为对照组。比较各组SAA/CRP、p-STAT3、NSE水平。两组间比较采用t检验。Pearson法分析两两变量的相关性。受试者操作特征(receiver operator characteristic,ROC)曲线分析SAA/CRP、p-STAT3、NSE水平对HFMD的预测价值。结果 重症组SAA/CRP、p-STAT3、NSE水平均高于轻症组(t=5.392、14.652、7.321,均P<0.001);危重症组SAA/CRP、p-STAT3、NSE水平均高于轻症组(t=6.314、13.844、8.830,均P<0.001);危重症组SAA/CRP、p-STAT3、NSE水平均高于重症组(t=2.691、4.989、4.318,均P<0.05)。SAA/CRP与p-STAT3、NSE水平呈正相关(r=0.792、0.813,P<0.001),NSE与p-STAT3水平呈正相关(r=0.700,P<0.001)。SAA/CRP、p-STAT3、NSE预测HFMD的曲线下面积(area under the curve,AUC)分别为0.856、0.876、0.887;三者联合预测的AUC为0.950。结论 HFMD患儿SAA/CRP、p-STAT3、NSE水平升高,三者联合检测在预测HFMD中具有重要价值。展开更多
目的:观察促红细胞生成素(EPO)对新生大鼠缺氧缺血性脑损伤后神经元特异性烯醇化酶表达的影响。方法:7日龄SD大鼠随机分成假手术对照组、EPO治疗组、缺氧缺血(HIBD)组。造模后在不同时间点检测脑质量损伤百分比、皮层脑组织HE染色、免...目的:观察促红细胞生成素(EPO)对新生大鼠缺氧缺血性脑损伤后神经元特异性烯醇化酶表达的影响。方法:7日龄SD大鼠随机分成假手术对照组、EPO治疗组、缺氧缺血(HIBD)组。造模后在不同时间点检测脑质量损伤百分比、皮层脑组织HE染色、免疫组化观察皮层神经元特异性烯醇化酶(NSE)的表达。结果:与假手术对照组相比较,HIBD组大鼠脑组织病理变化明显,EPO治疗组能明显改善大鼠脑组织形态变化和脑质量损伤百分比(P<0.05);HIBD组大鼠造模48 h后可见部分NSE阳性表达出现在神经元外,造模后第4天NSE的阳性表达达到高峰;在相同时间点EPO治疗组可明显减少NSE阳性表达颗粒在神经元间隙的出现;HIBD组大鼠在造模后48h和8 d NSE阳性细胞数目均少于同日龄对照组和EPO组(P<0.05)。结论:EPO在HIBD发生早期起到神经保护的作用,减少神经元的坏死,减轻缺氧缺血导致的脑功能损伤。展开更多
文摘Purpose: Neuron-specific enolase (NSE) of containing γ-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin.Method : We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (aa,aγ and γγ),and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of fluorometer.Result: Aqueous from all patients with Rb contained aa,ar and rr isoenzymes and presented strong postitive, the positive rate of NSE being 100% and its relative activity accounting for 45 ± 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats's diseases (4 cases),but in two
文摘目的 探讨手足口病(hand-foot-mouth disease,HFMD)患儿中血清淀粉样蛋白A/C反应蛋白(serum amyloid A/C reaction protein,SAA/CRP)、磷酸化信号转导和转录激活因子3(phosphorylated signal transducer and activator of transcription 3,p-STAT3)、神经元特异性烯醇化酶(neuron specific enolase,NSE)水平变化,为评估病情严重程度及预测价值提供科学依据。方法 将2022年3月至2024年3月收治的104例HFMD患儿分为轻症组、重症组、危重症组。选取同期入院进行健康体检的52例儿童作为对照组。比较各组SAA/CRP、p-STAT3、NSE水平。两组间比较采用t检验。Pearson法分析两两变量的相关性。受试者操作特征(receiver operator characteristic,ROC)曲线分析SAA/CRP、p-STAT3、NSE水平对HFMD的预测价值。结果 重症组SAA/CRP、p-STAT3、NSE水平均高于轻症组(t=5.392、14.652、7.321,均P<0.001);危重症组SAA/CRP、p-STAT3、NSE水平均高于轻症组(t=6.314、13.844、8.830,均P<0.001);危重症组SAA/CRP、p-STAT3、NSE水平均高于重症组(t=2.691、4.989、4.318,均P<0.05)。SAA/CRP与p-STAT3、NSE水平呈正相关(r=0.792、0.813,P<0.001),NSE与p-STAT3水平呈正相关(r=0.700,P<0.001)。SAA/CRP、p-STAT3、NSE预测HFMD的曲线下面积(area under the curve,AUC)分别为0.856、0.876、0.887;三者联合预测的AUC为0.950。结论 HFMD患儿SAA/CRP、p-STAT3、NSE水平升高,三者联合检测在预测HFMD中具有重要价值。
文摘目的:观察促红细胞生成素(EPO)对新生大鼠缺氧缺血性脑损伤后神经元特异性烯醇化酶表达的影响。方法:7日龄SD大鼠随机分成假手术对照组、EPO治疗组、缺氧缺血(HIBD)组。造模后在不同时间点检测脑质量损伤百分比、皮层脑组织HE染色、免疫组化观察皮层神经元特异性烯醇化酶(NSE)的表达。结果:与假手术对照组相比较,HIBD组大鼠脑组织病理变化明显,EPO治疗组能明显改善大鼠脑组织形态变化和脑质量损伤百分比(P<0.05);HIBD组大鼠造模48 h后可见部分NSE阳性表达出现在神经元外,造模后第4天NSE的阳性表达达到高峰;在相同时间点EPO治疗组可明显减少NSE阳性表达颗粒在神经元间隙的出现;HIBD组大鼠在造模后48h和8 d NSE阳性细胞数目均少于同日龄对照组和EPO组(P<0.05)。结论:EPO在HIBD发生早期起到神经保护的作用,减少神经元的坏死,减轻缺氧缺血导致的脑功能损伤。
