Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the trea...Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses.Therefore,it is impor-tant to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains.In this study,novel selenium-tellurium based nanoparticles(SeTeNPs)were synthesized and characterized.Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model.A total of 10 heifers were divided into experimental and control groups(5 animals each).After intramammary infection with methicillin resistant S.aureus(MRSA)and the development of clinical signs of mastitis,a dose of SeTeNPs was administered to all quarters in the experimental group.Results Based on in vitro tests,the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te,respectively,was used for application into the mammary gland.Three days after SeTeNPs administration,MRSA counts in the experimental group showed a significant reduction(P<0.01)compared to the control group.The inhibitory effect observed within the in vitro experiments was thus confirmed,resulting in the suppression of infection in ani-mals.Moreover,the superior biocompatibility of SeTeNPs in the organism was demonstrated,as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application,i.e.,mammary gland,compared to the control group(P>0.05).Additionally,the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected,indicating that the nanoparticles had no adverse effects on the organism.Conclusions Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the pres-ence of resistant bacteria.However,the current study is limited by its small sample size,making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs.Therefore,further research with a larger sample size is needed to validate these results.展开更多
基金Financial support from ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No.CZ.02.1.01/0.0/0.0/16_025/0007314)the assistance provided by the Research Infrastructure Nano Envi Cz, supported by the Ministry of Education, Youth and Sports of the Czech Republic under Project No. LM2018124+4 种基金Czech Nano Lab Research Infrastructure supported by MEYS CR (LM2023051)Grant Agency of Gregor Johann Mendel (C-MNG-23–002)further supported by the Ministry of Agriculture of the Czech Republic by Grant RO0523Internal Grant Agency of University of Veterinary Sciences Brno (223/2024/FVHE)the National Institute of Virology and Bacteriology project (Programme EXCELES, Project ID No. LX22NPO5103)-Funded by the European Union-Next Generation EU.
文摘Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses.Therefore,it is impor-tant to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains.In this study,novel selenium-tellurium based nanoparticles(SeTeNPs)were synthesized and characterized.Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model.A total of 10 heifers were divided into experimental and control groups(5 animals each).After intramammary infection with methicillin resistant S.aureus(MRSA)and the development of clinical signs of mastitis,a dose of SeTeNPs was administered to all quarters in the experimental group.Results Based on in vitro tests,the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te,respectively,was used for application into the mammary gland.Three days after SeTeNPs administration,MRSA counts in the experimental group showed a significant reduction(P<0.01)compared to the control group.The inhibitory effect observed within the in vitro experiments was thus confirmed,resulting in the suppression of infection in ani-mals.Moreover,the superior biocompatibility of SeTeNPs in the organism was demonstrated,as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application,i.e.,mammary gland,compared to the control group(P>0.05).Additionally,the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected,indicating that the nanoparticles had no adverse effects on the organism.Conclusions Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the pres-ence of resistant bacteria.However,the current study is limited by its small sample size,making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs.Therefore,further research with a larger sample size is needed to validate these results.
