Six overlapping DNA fragments from swine vesicular disease virus (SVDV) RNA were acquired by RT-PCR,nested PCR,and half-nested PCR.These fragments were individually cloned and sequenced.Comparing and analyzing of homo...Six overlapping DNA fragments from swine vesicular disease virus (SVDV) RNA were acquired by RT-PCR,nested PCR,and half-nested PCR.These fragments were individually cloned and sequenced.Comparing and analyzing of homologies of nucleotide and animo acid sequences by computer software,the DNA sequence homologies were 98.4% with SVDV J1′73,98.2% with SVDV H/3′76,98.2% with SVDV(Seechurn.P,et al),91.0% with SVDV NET/1/92;the amino acid sequence homoiogies were 98.9% with SVDV J1′73,98.9% with SVDV H/3′76,99.0% with SVDV (Seechurn P,et al.)96.9% with SVDV NET/1/92.展开更多
文摘Six overlapping DNA fragments from swine vesicular disease virus (SVDV) RNA were acquired by RT-PCR,nested PCR,and half-nested PCR.These fragments were individually cloned and sequenced.Comparing and analyzing of homologies of nucleotide and animo acid sequences by computer software,the DNA sequence homologies were 98.4% with SVDV J1′73,98.2% with SVDV H/3′76,98.2% with SVDV(Seechurn.P,et al),91.0% with SVDV NET/1/92;the amino acid sequence homoiogies were 98.9% with SVDV J1′73,98.9% with SVDV H/3′76,99.0% with SVDV (Seechurn P,et al.)96.9% with SVDV NET/1/92.
