AIM： TO study the effect of SNC19/ST14gene overexpression on invasion in vitro of colorectal cancer cells. METHODS： The adhesion of SlVC19/ST14gene-transfected cells to ECM was measured by MTT assay. The cell moveme...AIM： TO study the effect of SNC19/ST14gene overexpression on invasion in vitro of colorectal cancer cells. METHODS： The adhesion of SlVC19/ST14gene-transfected cells to ECM was measured by MTT assay. The cell movement was evaluated by wound healing assay. Cell invasion and migration were determined by invasion assay in vitro. RESULTS： SNC19/ST14 gene overexpression could enhance invasion of colorectal cancer cells in vitro significantly and influence early cell adherence to ECM, but could not change cell movement significantly. CONCLUSION： SNC19/ST14 gene overexpression increases the local invasion of colorectal cancer cells in vitro. 2005 The WJG Press and Elsevier Inc. All rights reserved.展开更多
ST14 is one of the type II transmembrane serine proteases that correlates with the process of tumor metastasis. The C-terminal catalytic region (900 bp) of ST14 was cloned into the expression vector pGEX-4T-2 and the ...ST14 is one of the type II transmembrane serine proteases that correlates with the process of tumor metastasis. The C-terminal catalytic region (900 bp) of ST14 was cloned into the expression vector pGEX-4T-2 and the positive plasmid pGEX-4T-2-ST14 was transformed into E. coli BL21, then cultured and induced with IPTG. The chaperonin GroEL was found to be tightly associated with the fusion protein and co-purified with it by regular GST affinity chromatography. A method for the removal of contaminating GroEL from GST-ST14 fusion protein was described, the purity of product was 96.2%. Enzyme activity assay indicated that this fusion protein had serine protease activity.展开更多
The BclI polymorphism within DXS52 (St14) was reported. It was composed of 4 allelic fragments 4.0 kb, 3.3 kb, 3.0 kb and 2.3 kb, The frequency of these fragments were 0.09, 0.12, 0.44 and 0.35 respectively in the Chi...The BclI polymorphism within DXS52 (St14) was reported. It was composed of 4 allelic fragments 4.0 kb, 3.3 kb, 3.0 kb and 2.3 kb, The frequency of these fragments were 0.09, 0.12, 0.44 and 0.35 respectively in the Chinese. The polymorphism provided the PIC of 0.66. DNA analysis of families, with hemophilia A showed that the confidence of the RFLPs was the same as the TaqI/St14 RFLPs and for carrier detection the former is much better than that of the TaqI/St14 RFLPs.展开更多
基金Supported by the National Natural Science Foundation of China,No.30200325
文摘AIM： TO study the effect of SNC19/ST14gene overexpression on invasion in vitro of colorectal cancer cells. METHODS： The adhesion of SlVC19/ST14gene-transfected cells to ECM was measured by MTT assay. The cell movement was evaluated by wound healing assay. Cell invasion and migration were determined by invasion assay in vitro. RESULTS： SNC19/ST14 gene overexpression could enhance invasion of colorectal cancer cells in vitro significantly and influence early cell adherence to ECM, but could not change cell movement significantly. CONCLUSION： SNC19/ST14 gene overexpression increases the local invasion of colorectal cancer cells in vitro. 2005 The WJG Press and Elsevier Inc. All rights reserved.
文摘ST14 is one of the type II transmembrane serine proteases that correlates with the process of tumor metastasis. The C-terminal catalytic region (900 bp) of ST14 was cloned into the expression vector pGEX-4T-2 and the positive plasmid pGEX-4T-2-ST14 was transformed into E. coli BL21, then cultured and induced with IPTG. The chaperonin GroEL was found to be tightly associated with the fusion protein and co-purified with it by regular GST affinity chromatography. A method for the removal of contaminating GroEL from GST-ST14 fusion protein was described, the purity of product was 96.2%. Enzyme activity assay indicated that this fusion protein had serine protease activity.
文摘The BclI polymorphism within DXS52 (St14) was reported. It was composed of 4 allelic fragments 4.0 kb, 3.3 kb, 3.0 kb and 2.3 kb, The frequency of these fragments were 0.09, 0.12, 0.44 and 0.35 respectively in the Chinese. The polymorphism provided the PIC of 0.66. DNA analysis of families, with hemophilia A showed that the confidence of the RFLPs was the same as the TaqI/St14 RFLPs and for carrier detection the former is much better than that of the TaqI/St14 RFLPs.
