SRO(Similar to radical-induced cell death 1)蛋白是植物特有的一类小蛋白家族转录因子,在植物抵御生物/非生物胁迫(盐害,干旱,高温,低温,水涝等)和生长发育中发挥着重要的作用。尽管SROs已在许多物种中进行了研究,但在马铃薯中仍缺...SRO(Similar to radical-induced cell death 1)蛋白是植物特有的一类小蛋白家族转录因子,在植物抵御生物/非生物胁迫(盐害,干旱,高温,低温,水涝等)和生长发育中发挥着重要的作用。尽管SROs已在许多物种中进行了研究,但在马铃薯中仍缺乏系统鉴定。基于马铃薯已公布的基因组数据对马铃薯SRO蛋白家族进行全基因组鉴定和生物信息学分析,共鉴定到9个St SROs家族成员,不均匀分布在5条染色体上,亚细胞定位预测该基因全部成员定位在细胞核上。启动子上游鉴定到了大量与生长发育、植物激素响应和生物/非生物胁迫有关的顺式作用元件,同时预测了调控该基因的miRNAs。该基因各成员对不同激素处理响应程度存在明显差异。上述结果为马铃薯St SROs潜在的分子功能提供了理论依据,表明该基因在马铃薯非生物胁迫方面可能具有重要意义。展开更多
Background SRO(Similar to RCD1)genes family is largely recognized for their importance in the growth,develop-ment,and in responding to environmental stresses.However,genome-wide identification and functional character...Background SRO(Similar to RCD1)genes family is largely recognized for their importance in the growth,develop-ment,and in responding to environmental stresses.However,genome-wide identification and functional characteri-zation of SRO genes from cotton species have not been reported so far.Results A total of 36 SRO genes were identified from four cotton species.Phylogenetic analysis divided these genes into three groups with distinct structure.Syntenic and chromosomal distribution analysis indicated uneven distribu-tion of GaSRO,GrSRO,GhSRO,and GbSRO genes on A2,D5 genomes,Gh-At,Gh-Dt,Gb-At,and Gb-Dt subgenomes,respectively.Gene duplication analysis revealed the presence of six duplicated gene pairs among GhSRO genes.In promoter analysis,several elements responsive to the growth,development and hormones were found in GhSRO genes,implying gene induction during cotton growth and development.Several miRNAs responsive to plant growth and abiotic stress were predicted to target 12 GhSRO genes.Organ-specific expression profiling demonstrated the roles of GhSRO genes in one or more tissues.In addition,specific expression pattern of some GhSRO genes dur-ing ovule development depicted their involvement in these developmental processes.Conclusion The data presented in this report laid a foundation for understanding the classification and functions of SRO genes in cotton.展开更多
文摘SRO(Similar to radical-induced cell death 1)蛋白是植物特有的一类小蛋白家族转录因子,在植物抵御生物/非生物胁迫(盐害,干旱,高温,低温,水涝等)和生长发育中发挥着重要的作用。尽管SROs已在许多物种中进行了研究,但在马铃薯中仍缺乏系统鉴定。基于马铃薯已公布的基因组数据对马铃薯SRO蛋白家族进行全基因组鉴定和生物信息学分析,共鉴定到9个St SROs家族成员,不均匀分布在5条染色体上,亚细胞定位预测该基因全部成员定位在细胞核上。启动子上游鉴定到了大量与生长发育、植物激素响应和生物/非生物胁迫有关的顺式作用元件,同时预测了调控该基因的miRNAs。该基因各成员对不同激素处理响应程度存在明显差异。上述结果为马铃薯St SROs潜在的分子功能提供了理论依据,表明该基因在马铃薯非生物胁迫方面可能具有重要意义。
文摘Background SRO(Similar to RCD1)genes family is largely recognized for their importance in the growth,develop-ment,and in responding to environmental stresses.However,genome-wide identification and functional characteri-zation of SRO genes from cotton species have not been reported so far.Results A total of 36 SRO genes were identified from four cotton species.Phylogenetic analysis divided these genes into three groups with distinct structure.Syntenic and chromosomal distribution analysis indicated uneven distribu-tion of GaSRO,GrSRO,GhSRO,and GbSRO genes on A2,D5 genomes,Gh-At,Gh-Dt,Gb-At,and Gb-Dt subgenomes,respectively.Gene duplication analysis revealed the presence of six duplicated gene pairs among GhSRO genes.In promoter analysis,several elements responsive to the growth,development and hormones were found in GhSRO genes,implying gene induction during cotton growth and development.Several miRNAs responsive to plant growth and abiotic stress were predicted to target 12 GhSRO genes.Organ-specific expression profiling demonstrated the roles of GhSRO genes in one or more tissues.In addition,specific expression pattern of some GhSRO genes dur-ing ovule development depicted their involvement in these developmental processes.Conclusion The data presented in this report laid a foundation for understanding the classification and functions of SRO genes in cotton.
