Pentatricopeptide repeat(PPR)proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression.Here,we report the function of PPR21 in mitochondrial intro...Pentatricopeptide repeat(PPR)proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression.Here,we report the function of PPR21 in mitochondrial intron splicing and its role in maize kernel development.PPR21 is a typical P-type PPR protein targeted to mitochondria.The ppr21 mutants are arrested in embryogenesis and endosperm development,leading to embryo lethality.Null mutations of PPR21 reduce the splicing efficiency of nad2 intron 1,2,and 4 and impair the assembly and activity of mitochondrial complex I.Previous studies show that the P-type PPR protein EMP12 is required for the splicing of identical introns.However,our protein interaction analyses reveal that PPR21 does not interact with EMP12.Instead,both PPR21 and EMP12 interact with the small MutS-related(SMR)domain-containing PPR protein 1(PPR-SMR1)and the short P-type PPR protein 2(SPR2).PPR-SMR1 interacts with SPR2,and both proteins are required for the splicing of many introns in mitochondria,including nad2 intron 1,2,and 4.These results suggest that a PPR21-(PPR-SMR1/SPR2)-EMP12 complex is involved in the splicing of nad2 introns in maize mitochondria.展开更多
This paper uses an innovative improved artificial bee colony(IABC)algorithm to aid in the fabrication of a highly responsive phasemodulation surface plasmon resonance(SPR)biosensor.In this biosensor’s sensing structu...This paper uses an innovative improved artificial bee colony(IABC)algorithm to aid in the fabrication of a highly responsive phasemodulation surface plasmon resonance(SPR)biosensor.In this biosensor’s sensing structure,a double-layer Ag-Au metal film is combined with a blue phosphorene/transition metal dichalcogenide(BlueP/TMDC)hybrid structure and graphene.In the optimization function of the IABC method,the reflectivity at resonance angle is incorporated as a constraint to achieve high phase sensitivity.The performance of the Ag-Au-BlueP/TMDC-graphene heterostructure as optimized by the IABC method is compared with that of a similar structure optimized using the traditional ABC algorithm.The results indicate that optimization using the IABC method gives significantly more phase sensitivity,together with lower reflectivity,than can be achieved with the traditional ABC method.The highest phase sensitivity of 3.662×10^(6) °/RIU is achieved with a bilayer of BlueP/WS2 and three layers of graphene.Moreover,analysis of the electric field distribution demonstrates that the optimal arrangement can be utilized for enhanced detection of small biomolecules.Thus,given the exceptional sensitivity achieved,the proposed method based on the IABC algorithm has great promise for use in the design of high-performance SPR biosensors with a variety of multilayer structures.展开更多
Serial-parallel manipulators are of great interest to academic community in recent years,especially those composed of classical parallel mechanisms.There have been many studies around 2(3RPS)and 2(3SPR)S-PMs,but unfor...Serial-parallel manipulators are of great interest to academic community in recent years,especially those composed of classical parallel mechanisms.There have been many studies around 2(3RPS)and 2(3SPR)S-PMs,but unfortunately their inverse kinematics have not yet been resolved.This paper discovers that the unknown kinematic parameters of middle platform are responsible for the unresolvable of inverse kinematics,meanwhile the unknown kinematic parameters of middle platform also have huge coupling relationships.Therefore,to break through this challenges,the huge coupling relationships are decoupled layer by layer,the kinematic parameters of middle platform are solved by combining Sylvester's elimination method,and the inverse displacements of 2(3RPS)and 2(3SPR)S-PMs are obtained subsequently.This paper not only solves the inverse kinematics of classical 2(3RPS)and 2(3SPR)S-PMs,but also reveals the essence of the inverse kinematics of general(3-DOF)+(3-DOF)6-DOF S-PMs and proposes a corresponding solution.展开更多
基金supported by the National Natural Science Foundation of China(32072126 and 32230075)the Shandong Provincial Natural Science Foundation(ZR2019MC005).
文摘Pentatricopeptide repeat(PPR)proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression.Here,we report the function of PPR21 in mitochondrial intron splicing and its role in maize kernel development.PPR21 is a typical P-type PPR protein targeted to mitochondria.The ppr21 mutants are arrested in embryogenesis and endosperm development,leading to embryo lethality.Null mutations of PPR21 reduce the splicing efficiency of nad2 intron 1,2,and 4 and impair the assembly and activity of mitochondrial complex I.Previous studies show that the P-type PPR protein EMP12 is required for the splicing of identical introns.However,our protein interaction analyses reveal that PPR21 does not interact with EMP12.Instead,both PPR21 and EMP12 interact with the small MutS-related(SMR)domain-containing PPR protein 1(PPR-SMR1)and the short P-type PPR protein 2(SPR2).PPR-SMR1 interacts with SPR2,and both proteins are required for the splicing of many introns in mitochondria,including nad2 intron 1,2,and 4.These results suggest that a PPR21-(PPR-SMR1/SPR2)-EMP12 complex is involved in the splicing of nad2 introns in maize mitochondria.
基金funded by the National Natural Science Foundation of China(Grant No.52375547)the Natural Science Foundation of Chongqing,China(Grant Nos.CSTB2022NSCQ-BHX0736 and CSTB2022NSCQ-MSX1523)the Chongqing Scientific Institution Incentive Performance Guiding Special Projects(Grant No.CSTB2024JXJL-YFX0034).
文摘This paper uses an innovative improved artificial bee colony(IABC)algorithm to aid in the fabrication of a highly responsive phasemodulation surface plasmon resonance(SPR)biosensor.In this biosensor’s sensing structure,a double-layer Ag-Au metal film is combined with a blue phosphorene/transition metal dichalcogenide(BlueP/TMDC)hybrid structure and graphene.In the optimization function of the IABC method,the reflectivity at resonance angle is incorporated as a constraint to achieve high phase sensitivity.The performance of the Ag-Au-BlueP/TMDC-graphene heterostructure as optimized by the IABC method is compared with that of a similar structure optimized using the traditional ABC algorithm.The results indicate that optimization using the IABC method gives significantly more phase sensitivity,together with lower reflectivity,than can be achieved with the traditional ABC method.The highest phase sensitivity of 3.662×10^(6) °/RIU is achieved with a bilayer of BlueP/WS2 and three layers of graphene.Moreover,analysis of the electric field distribution demonstrates that the optimal arrangement can be utilized for enhanced detection of small biomolecules.Thus,given the exceptional sensitivity achieved,the proposed method based on the IABC algorithm has great promise for use in the design of high-performance SPR biosensors with a variety of multilayer structures.
基金Supported by National Natural Science Foundation of China(Grant No.52275033)National Natural Science Youth Foundation of China(Grant No.52205033)Hebei Provincial Natural Science Foundation of China(Grant No.E2021203019)。
文摘Serial-parallel manipulators are of great interest to academic community in recent years,especially those composed of classical parallel mechanisms.There have been many studies around 2(3RPS)and 2(3SPR)S-PMs,but unfortunately their inverse kinematics have not yet been resolved.This paper discovers that the unknown kinematic parameters of middle platform are responsible for the unresolvable of inverse kinematics,meanwhile the unknown kinematic parameters of middle platform also have huge coupling relationships.Therefore,to break through this challenges,the huge coupling relationships are decoupled layer by layer,the kinematic parameters of middle platform are solved by combining Sylvester's elimination method,and the inverse displacements of 2(3RPS)and 2(3SPR)S-PMs are obtained subsequently.This paper not only solves the inverse kinematics of classical 2(3RPS)and 2(3SPR)S-PMs,but also reveals the essence of the inverse kinematics of general(3-DOF)+(3-DOF)6-DOF S-PMs and proposes a corresponding solution.
