摘要
目的探讨化脓性脑膜炎肺炎链球菌spr1759蛋白的理化性质并预测其功能。方法从NCBI数据库中获得spr1759蛋白的相关序列信息,运用ProtParam以及ProtScale软件对spr1759的理化特性及具体的亲疏水性予以分析,运用NetPhos、TMHMM分析spr1759的磷酸化位点及跨膜区结构,分别运用SWISS-MODEL、SOPMA、ABCpred对spr1759的二级及三级结构的具体情况予以预测,用SYFPEITHI软件进行抗原表位的预测分析,STRING预测spr1759的相互作用蛋白,并对其生物过程进行功能富集分析。结果spr1759蛋白是一种亲水蛋白,等电点为5.11,有一个跨膜区,其全部包含一个跨膜结构。肺炎链球菌spr1759蛋白属于LytRcpsApsr超家族,可能是一种参与阴离子细胞壁聚合物合成的酶。肺炎链球菌spr1759蛋白的氨基酸序列与B.炭疽杆菌的BAS5115蛋白和枯草芽孢杆菌的DJ971211蛋白高度同源,全长序列相似性为35.7%和32.5%。结论肺炎链球菌spr1759蛋白属于亲水性蛋白,具有跨膜结构,可能具有阴离子细胞壁多聚物合成相关酶学功能。
Objective To investigate the physicochemical properties and functional prediction of S.pneumoniae spr1759 protein in purulent meningitis.Methods The relevant sequence information of spr1759 protein was obtained from NCBI database.The physical and chemical characteristics and hydrophobicity of spr1759 protein were analyzed by ProtParam and ProtScale.The phosphorylation site and transmembrane region structure of spr1759 were analyzed by NetPhos and TMHMM.The secondary structure and tertiary structure of spr1759 protein were predicted by SOPMA,SWISS-MODEL,and ABCpred,respectively.The epitope prediction analysis was performed using SYFPEITHI software.The interacting protein of spr1759 was predicted using STRING,and its biological process was functionally enriched and analyzed.Results spr1759 protein was a hydrophilic protein with an isoelectric point of 5.11 and a transmembrane region,all of which contained a transmembrane structure.S.pneumoniae spr1759 protein belonged to the LytRcpsApsr superfamily and may be an enzyme involved in anionic cell wall polymer synthesis.The amino acid sequence of S.pneumoniae spr1759 protein was highly homologous to B.anthracis BAS5115 protein and B.subtilis DJ971211 protein,with 35.7%and 32.5%full-length sequence similarity.Conclusion Streptococcus pneumoniae spr1759 protein is a hydrophilic protein with transmembrane structure and may have enzymatic functions related to anionic cell wall polymer synthesis.
作者
孙旸园
冯晓伟
SUN Yangyuan;FENG Xiaowei(Baoji People’s Hospital,Baoji 721000,China)
出处
《中国实用神经疾病杂志》
2025年第1期18-22,共5页
Chinese Journal of Practical Nervous Diseases
基金
陕西省卫生健康科研基金项目(编号:2021JQ-989)。
关键词
脑膜炎
肺炎链球菌
spr1759蛋白
理化性质
蛋白功能预测
Meningitis
Streptococcus pneumoniae
spr1759 protein
Physicochemical properties
Protein function prediction
