文章对Zr-Sn系锆合金中间工序管坯采用两种退火工序制备,采用扫描电镜(Scanning Electron Microscope,SEM)对其微观组织形貌进行观察,并对其第二相尺寸进行统计,最后对其腐蚀性能进行分析。SPPs统计结果表明,退火工序的选择对SPPs的尺...文章对Zr-Sn系锆合金中间工序管坯采用两种退火工序制备,采用扫描电镜(Scanning Electron Microscope,SEM)对其微观组织形貌进行观察,并对其第二相尺寸进行统计,最后对其腐蚀性能进行分析。SPPs统计结果表明,退火工序的选择对SPPs的尺寸形貌有显著影响,A样品内析出相的数量明显多于样品B,且尺寸相对较大。腐蚀结果表明,采用A工序退火的TREX管坯更耐均匀腐蚀。展开更多
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short...Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.展开更多
Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the ant...Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst.and Kit.,A.grandifolia Friv.and A.millefolium L.The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis,and the results were consistent with the spectrophotometrically determined total phenolic(TP:125.42-191.98 mg/g)and total flavonoid(TF:47.34-180.02 mg/g)contents.All the extracts were tested for their antioxidant activity using DPPH and ABTS•+radical scavenging assay,as well as ferrous ion chelating ability and reducing power tests.All the extracts showed moderate antioxidant activity,compared to the reference substance BHT.Additionally,the antibacterial activity of the extracts was evaluated against major food-borne pathogens,showing moderate antimicrobial effects.展开更多
Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al....Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al.,2025).However,its rapid softening severely limits shelf life,causing substantial economic losses during transport and storage.Recently,the enhanced shelf-life can be generated by compromising the key ripening regulators,such as RIN,but other fruit quality traits including flavor and color also can be impaired concurrently(Kitagawa et al.,2005).展开更多
Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100...Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.展开更多
文摘文章对Zr-Sn系锆合金中间工序管坯采用两种退火工序制备,采用扫描电镜(Scanning Electron Microscope,SEM)对其微观组织形貌进行观察,并对其第二相尺寸进行统计,最后对其腐蚀性能进行分析。SPPs统计结果表明,退火工序的选择对SPPs的尺寸形貌有显著影响,A样品内析出相的数量明显多于样品B,且尺寸相对较大。腐蚀结果表明,采用A工序退火的TREX管坯更耐均匀腐蚀。
文摘目的:通过单细胞RNA测序阐明免疫细胞与纤维环细胞相互作用的时空动态特征。方法:实验采用12只8周龄雄性Sprague Dawley大鼠,并随机分为3组:正常对照组(NT)、针刺退变7d组(IDD7)及针刺退变14d组(IDD14),每组三只大鼠的椎间盘组织合并作为一个scRNA-seq样本(即NT/IDD7/IDD14各1个样本,n=3大鼠/组)。椎间盘组织经过混合的多种胶原酶消化后,并未进行流式分选,直接利用BD Rhapsody平台,对3个样本开展了单细胞RNA测序(scRNA-seq)。通过以下流程解析细胞动态变化:采用Seurat对原始计数矩阵进行质量控制、标准化及批次校正(Harmony算法整合),保留基因表达均值用于后续分析。通过FindVariableFeatures筛选2000个高变基因,经主成分分析(principal component analysis,PCA)降维后,采用Louvain聚类算法(分辨率0.2)对细胞进行分群,并结合SingleR工具进行细胞类型注释,获取各亚群的标志基因。对比正常对照组(NT)、针刺退变7d组(IDD7)及针刺退变14d组(IDD14)间的差异表达基因(筛选阈值:|logFC|>0.5,P<0.05)。使用基于R语言开发的生物信息学软件包ClusterProfiler与enrichR软件分别对差异基因进行GO(gene ontology)生物学过程与KEGG(kyoto encyclopedia of genes and genomes)通路富集分析(P<0.05,基因数≥2),筛选显著相关通路。基于Monocle3构建伪时间轨迹,将整合数据转换为cell_data_set对象,经UMAP(uniform manifold approximation and projection)降维与Louvain聚类后,利用learn_graph与order_cells函数揭示细胞状态演化路径。运用CellChat与CellPhoneDB分析配体-受体相互作用,计算细胞间通讯概率,可视化关键信号通路(如细胞因子、生长因子)在NT与IDD组间的动态变化。采用免疫荧光技术对CD68(巨噬细胞标记物)、SPP1、CD44等蛋白的共定位表达进行定量分析。结果:单细胞数据鉴定出9个主要细胞群:纤维环细胞(NP)、纤维环细胞(AF)、巨噬细胞(Mφ)、单核细胞、中性粒细胞、T细胞、成纤维细胞、内皮细胞及平滑肌细胞。74个基因持续差异表达,主要与免疫细胞趋化相关。并系统解析了纤维环细胞的时序性分子变化。纤维环细胞存在修复型(Fibro-1/3)与耗竭型(Fibro-2)两极分化,为精准干预提供靶群依据。拟时序T1期可能(对应IDD7d)是应激保护向纤维化转化的关键节点,为阻断不可逆损伤提供治疗窗口期。细胞互作动态演变:纤维环亚群中耗竭型Fibro-2与巨噬细胞的互作数最多,SPP1信号轴由Fibro-2主导发送,Mφ-3为主要接收者,该通路可能驱动纤维环细胞向促纤维化表型转化,提示其可能参与基质破坏和纤维化重塑进程。结论:纤维环细胞可能通过SPP1-CD44信号轴增强与巨噬细胞的相互作用,进而激活炎症反应并加速基质降解。这一发现揭示了椎间盘退变过程中的关键细胞受体-配体对,为IDD的精准干预提供了新的潜在治疗靶点。
文摘北斗三号卫星导航系统(BeiDou-3 Navigation Satellite System,BDS-3)于2020年7月正式开通全球导航定位服务.目前,BDS-3在B1I、B3I、B1C、B2a和B2b频点提供公开服务.伪距单点定位(single point positioning,SPP)因其成本低、算法简单等特点,广泛应用于车辆导航与智能终端定位等领域.为系统评估BDS-3各频点单频SPP性能,并探究北斗全球电离层延迟修正模型(BDS global ionospheric model,BDGIM)的性能,本文构建了适用于BDS-3全频点的单频SPP模型,基于22个全球均匀分布测站的观测数据,对比分析了BDGIM与GPS Klobuchar模型(GPSK8)在太阳活动活跃期与平静期对定位精度的影响.实验结果表明,在全球尺度上,BDGIM性能总体优于GPSK8,在平静期和活跃期的定位精度提升分别为15.6%和11.5%,且在高、低纬度地区改善尤为显著.各频点的单频SPP结果显示,B1C和B1I定位性能最优,3D方向精度分别为3.31 m和3.39 m.
基金supported by the National Research Foundation of Korea(2020R1F1A1074155).
文摘Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.
基金supported by the Serbian Ministry of Education,Science and Technological Development(Agreements No.451-03-136/2025-03/200122 and 451-03-136/2025-03/200378).
文摘Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst.and Kit.,A.grandifolia Friv.and A.millefolium L.The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis,and the results were consistent with the spectrophotometrically determined total phenolic(TP:125.42-191.98 mg/g)and total flavonoid(TF:47.34-180.02 mg/g)contents.All the extracts were tested for their antioxidant activity using DPPH and ABTS•+radical scavenging assay,as well as ferrous ion chelating ability and reducing power tests.All the extracts showed moderate antioxidant activity,compared to the reference substance BHT.Additionally,the antibacterial activity of the extracts was evaluated against major food-borne pathogens,showing moderate antimicrobial effects.
基金supported by the Natural Science Foundation of China(Grant Nos.32172544,32441071)the special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science(Grant No.R2023PY-JG003)+4 种基金the earmarked fund for CARS(Grant No.CARS-31-01)Guangdong Special Support Program(Grant No.NYLJ2024010)Guangdong S&T Program(Grant No.2025B0202070005)IAEA CRP D23033the Project from Guangzhou Municipal Science and Technology Bureau(2023B03J0991).
文摘Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al.,2025).However,its rapid softening severely limits shelf life,causing substantial economic losses during transport and storage.Recently,the enhanced shelf-life can be generated by compromising the key ripening regulators,such as RIN,but other fruit quality traits including flavor and color also can be impaired concurrently(Kitagawa et al.,2005).
基金Baoding Science and Technology Plan Project(Project No.:2341ZF214)。
文摘Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.
