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细菌素免疫蛋白调控伤寒沙门菌耐药和生物膜的形成 被引量:3
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作者 陈珉池 刘锐 +2 位作者 颜冬梅 赵昕 黄新祥 《江苏大学学报(医学版)》 CAS 2017年第6期461-465,共5页
目的:研究伤寒沙门菌SPI-8编码的两种细菌素免疫蛋白的功能。方法:用自杀质粒p GMB151介导的同源重组法,制备细菌素免疫蛋白基因缺陷变异株(△t3036,△t3038,△t3036△t3038);采用药敏纸片法检测细菌对8种抗菌药物的抵抗能力;结晶紫染... 目的:研究伤寒沙门菌SPI-8编码的两种细菌素免疫蛋白的功能。方法:用自杀质粒p GMB151介导的同源重组法,制备细菌素免疫蛋白基因缺陷变异株(△t3036,△t3038,△t3036△t3038);采用药敏纸片法检测细菌对8种抗菌药物的抵抗能力;结晶紫染色法观察细菌生物膜的形成。结果:通过PCR验证及序列比对,伤寒沙门菌细菌素免疫蛋白基因缺陷变异株制备成功;药敏实验结果显示,△t3038和△t3036△t3038变异株对氨苄西林、庆大霉素、多黏菌素B、环丙沙星、卡那霉素、亚胺培南等6种抗菌药物的抵抗能力明显变弱(P均<0.01),△t3036对8种抗菌药物的抵抗能力均无明显变化;生物膜形成实验结果显示,与野生株相比,△t3036生物膜形成能力增强(P<0.05),△t3038和△t3036△t3038生物膜形成能力明显减弱(P均<0.01),而△t3036△t3038生物膜形成能力较△t3038减弱。结论:伤寒沙门菌细菌素免疫蛋白T3036和T3038均能够促进细菌生物膜形成,T3038能够增强细菌对抗菌药物的抵御。 展开更多
关键词 细菌素免疫蛋白 伤寒沙门菌 spi-8 耐药 生物膜
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A remote sensing-based agricultural drought indicator and its implementation over a semi-arid region, Jordan 被引量:3
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作者 Khaled HAZAYMEH Quazi K HASSAN 《Journal of Arid Land》 SCIE CSCD 2017年第3期319-330,共12页
The objective of the study was to develop a remote sensing (i.e., Landsat-8 and MODIS)-based agricultural drought indicator (ADI) at 30-m spatial resolution and 8-day temporal resolution and also to evaluate its p... The objective of the study was to develop a remote sensing (i.e., Landsat-8 and MODIS)-based agricultural drought indicator (ADI) at 30-m spatial resolution and 8-day temporal resolution and also to evaluate its performance over a heterogeneous agriculture dominant semi-arid region in Jordan. Firstly, we used principal component analysis (PCA) to evaluate the correlations among six commonly used remote sensing-derived agricultural drought related variables. The variables included normalized difference water index (NDWI), normalized difference vegetation index (NDVI), visible and shortwave drought index (VSDI), normalized multiband drought index (NMDI), moisture stress index (MSI), and land surface temperature (LST). Secondly, we integrated the relatively less correlated variables (that were found to be NDWI, VSDI, and LST) to generate four agricultural drought categories/conditions (i.e., wet, mild drought, moderate drought, and severe drought). Finally, we evaluated the ADI maps against a set of 8-day ground-based standardized precipitation index values (i.e., SPI-I, SPI-2, ..., SPLS) by use of confusion matrices and observed the best results for SPI-4 (i.e., overall accuracy and Kappa-values were 83% and 76%, respectively) and SPI-5 (i.e., overall accuracy and Kappa-values were 85% and 78%, respectively). The results demonstrated that the method would be valuable for monitoring agricultural drought conditions in semi-arid regions at both a reasonably high spatial resolution (i.e., 30-m) and a short time period (i.e., 8-day). 展开更多
关键词 spatio-temporal image fusion model (STI-FM) land surface temperature (LST) surface reflectance standardizedprecipitation index (SPI) Landsat-8 MODIS
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