摘要
目的:研究伤寒沙门菌SPI-8编码的两种细菌素免疫蛋白的功能。方法:用自杀质粒p GMB151介导的同源重组法,制备细菌素免疫蛋白基因缺陷变异株(△t3036,△t3038,△t3036△t3038);采用药敏纸片法检测细菌对8种抗菌药物的抵抗能力;结晶紫染色法观察细菌生物膜的形成。结果:通过PCR验证及序列比对,伤寒沙门菌细菌素免疫蛋白基因缺陷变异株制备成功;药敏实验结果显示,△t3038和△t3036△t3038变异株对氨苄西林、庆大霉素、多黏菌素B、环丙沙星、卡那霉素、亚胺培南等6种抗菌药物的抵抗能力明显变弱(P均<0.01),△t3036对8种抗菌药物的抵抗能力均无明显变化;生物膜形成实验结果显示,与野生株相比,△t3036生物膜形成能力增强(P<0.05),△t3038和△t3036△t3038生物膜形成能力明显减弱(P均<0.01),而△t3036△t3038生物膜形成能力较△t3038减弱。结论:伤寒沙门菌细菌素免疫蛋白T3036和T3038均能够促进细菌生物膜形成,T3038能够增强细菌对抗菌药物的抵御。
Objective ： To study the function of two bacteriocin immunity proteins encoded by SPI-8 ofSa Irnonll a enteric a serovar Typhi. Metliods ： The gene deleted mutants of bacteriocin immunity proteins（46036, 46038 and 46036z／6038） were constructed by homologous recombination mediated by the suicide plasmid pGMB151. The resistance of bacteria to 8 antimicrobial agents was separately tested with the K-B method. Crystal violet staining was used to assess the formation of bacterial biofilm. Results ：Based on the results of PCR verification and sequence alignment, the gene deleted mutants of bacteriocin immunity protein were successfully constructed. The results of drug sensitivity test showed that resistance of △ t3038and △t3036 △t3038 to ampicillin , gentamicin , polymyxin B , ciprofloxacin , kanamycin , and imipenem were significantly attenuated（ both P 〈0. 01） . However, there was no significant difference be-tween △t3036 and the wild type strain in resistance to all of the 8 antimicrobial agents. The result of thebiofilm formation test showed compared with the wild type strain, the capability of biofilm formation by4 △63036 was markedly enhanced （ P 〈 0. 05 ） , but the capability of biofilm formation by △t3038 and △t3036△t3038 was significantly reduced （both P 〈0. 01） . Furthermore, the capability of biofilm forma-tion by 4 6036/／6038 was significantly reduced than that of 4 6038. Conclusion ： The bacteriocin im-munity proteins T3036 and T3038 of S. Typhi could promote the formation of bacterial biofilm, andT3038 could also enhance the antibiotic resistance of bacteria.
出处
《江苏大学学报（医学版）》
CAS
2017年第6期461-465,共5页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金资助项目(81371780)
关键词
细菌素免疫蛋白
伤寒沙门菌
SPI-8
耐药
生物膜
bacteriocin immunity protein
Salmonella enterica serovar Typhi
SPI-8
drug resist-ance
biofilm
