目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期...目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期临床个体化干预提供参考。方法选取2020年3月—2025年3月河北省胸科医院收治的TRALI患者249例为研究对象,根据输血后30 d内预后情况,分别纳入预后良好组(178例)、预后不良组(71例)。比较2组一般临床资料及血清SP-D、MUC1、A20水平,Logistic回归分析血清SP-D、MUC1、A20水平对TRALI患者预后不良的影响因素,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SP-D、MUC1、A20水平单独及联合检测对预后不良的预测效能,并采用一致性分析进行外部验证。结果2组输血次数、发血至输血时间、过敏史、急性生理与慢性健康评分系统Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分比较,差异有统计学意义(P<0.05);预后不良组血清SP-D、MUC1、A20水平分别为(89.54±21.36)g/L、(22.97±5.14)kU/L、(14.53±1.96)mg/L,明显高于预后良好组的(78.61±18.05)g/L、(16.28±4.32)kU/L、(12.67±1.84)mg/L,差异有统计学意义(P<0.05);Logistic回归分析显示,输血次数、发血至输血时间、过敏史均是TRALI患者预后不良的影响因素(P<0.05),APACHEⅡ评分及血清SP-D、MUC1、A20均与TRALI患者预后不良显著相关(P<0.05);ROC分析显示,血清SP-D、MUC1、A20联合预测预后不良的曲线下面积(area under the curve,AUC)为0.904(95%CI:0.860~0.938),优于各指标单独预测价值(Z=2.507、3.016、3.042,均P<0.05),且外部验证显示,联合预测预后不良与临床实际的符合率为95.00%,Kappa值为0.870(95%CI:0.617~0.982)差异有统计学意义(P<0.05)。结论血清SP-D、MUC1、A20均是TRALI患者预后不良的独立影响因素,联合检测对预后不良具有较高预测价值,可作为TRALI患者预后的潜在预测因子,并可指导临床工作。展开更多
Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,red...Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency.展开更多
根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上...根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。通报统计分析1.通报数量2026年1月,共有42个国家(地区)或组织向WTO秘书处发送194件SPS通报,提交通报数量位列前十位的成员依次是:巴西(23件)、巴拿马(20件)、欧盟(17件)、日本(17件)、哥斯达黎加(8件)、坦桑尼亚(8件)、印度(7件)、尼加拉瓜(7件)、智利(6件)和中国台湾(6件)。展开更多
根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上...根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。展开更多
In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions....In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang.展开更多
Hyperactivation of DNA repairing pathway is highly associated with the chemosensitivity and chemoresistance of cancer cells.In this manuscript,guided by cascaded one strain many compounds-global natural products socia...Hyperactivation of DNA repairing pathway is highly associated with the chemosensitivity and chemoresistance of cancer cells.In this manuscript,guided by cascaded one strain many compounds-global natural products social molecular networking(OSMAC-GNPS)strategy,a pair of epimeric environmental-induced metabolites were isolated from Aspergillus sp.EGF 15-0-3.Structurally,sterpiperazines A(1)and B(2)represent the first steroid-based indole alkaloids with unprecedented backbones.Biologically,compound 1 could be identified as a novel tyrosyl-DNA phosphodiesterase 1(Tdp1)inhibitor with a unique mechanism distinct from the reported modulators,and was able to significantly enhance the sensitivity of NCI-H460 cells to the clinic chemotherapeutic drug through inhibiting the DNA repairment and enhanced the DNA damage of cancer cells.展开更多
文摘目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期临床个体化干预提供参考。方法选取2020年3月—2025年3月河北省胸科医院收治的TRALI患者249例为研究对象,根据输血后30 d内预后情况,分别纳入预后良好组(178例)、预后不良组(71例)。比较2组一般临床资料及血清SP-D、MUC1、A20水平,Logistic回归分析血清SP-D、MUC1、A20水平对TRALI患者预后不良的影响因素,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SP-D、MUC1、A20水平单独及联合检测对预后不良的预测效能,并采用一致性分析进行外部验证。结果2组输血次数、发血至输血时间、过敏史、急性生理与慢性健康评分系统Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分比较,差异有统计学意义(P<0.05);预后不良组血清SP-D、MUC1、A20水平分别为(89.54±21.36)g/L、(22.97±5.14)kU/L、(14.53±1.96)mg/L,明显高于预后良好组的(78.61±18.05)g/L、(16.28±4.32)kU/L、(12.67±1.84)mg/L,差异有统计学意义(P<0.05);Logistic回归分析显示,输血次数、发血至输血时间、过敏史均是TRALI患者预后不良的影响因素(P<0.05),APACHEⅡ评分及血清SP-D、MUC1、A20均与TRALI患者预后不良显著相关(P<0.05);ROC分析显示,血清SP-D、MUC1、A20联合预测预后不良的曲线下面积(area under the curve,AUC)为0.904(95%CI:0.860~0.938),优于各指标单独预测价值(Z=2.507、3.016、3.042,均P<0.05),且外部验证显示,联合预测预后不良与临床实际的符合率为95.00%,Kappa值为0.870(95%CI:0.617~0.982)差异有统计学意义(P<0.05)。结论血清SP-D、MUC1、A20均是TRALI患者预后不良的独立影响因素,联合检测对预后不良具有较高预测价值,可作为TRALI患者预后的潜在预测因子,并可指导临床工作。
文摘Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency.
文摘根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。通报统计分析1.通报数量2026年1月,共有42个国家(地区)或组织向WTO秘书处发送194件SPS通报,提交通报数量位列前十位的成员依次是:巴西(23件)、巴拿马(20件)、欧盟(17件)、日本(17件)、哥斯达黎加(8件)、坦桑尼亚(8件)、印度(7件)、尼加拉瓜(7件)、智利(6件)和中国台湾(6件)。
文摘根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。
基金financially supported by Xinjiang Major Science and Technology Projects,China (2023A02009)。
文摘In China,Xinjiang is a relatively independent epidemic region of wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,due to great population genetic divergence of Xinjiang with other inland epidemic regions.In this region,race evolution was usually slower than inland populations.However,many new races have recently been found,and therefore,it is necessary for more understanding of the virulence evolution of the Xinjiang population.So,in this study,a 65 sexual progenies,derived from a Xinjiang single-urediospore isolate BGTB-1 of P.striiformis f.sp.tritici by selfing on alternate host barberry(Berberis aggregata).It was phenotyped on the 25 single Yr lines,and genotyped by 19 kompetitive allele-specific PCRsingle nucleotide polymorphism(KASP-SNP) markers.As a result,the 65 progenies were identified as 56 various virulence patterns(VPs),and neither of which was identical to the parental isolate,showed 100% virulence variation.Compared with the parental isolate,of all progenies,39(60.0%) had increased virulence,and 18(27.7%) had decreased virulence.All progenies exhibited avirulence to Yr10,Yr15,Yr32,and YrTr1 loci,and avirulence and virulence segregation at the remaining 21 Yr resistance loci.The results showed avirulence to Yr5,Yr7,and Yr76(A:V≈3:1) loci is controlled by a single dominant gene,and that to Yr6,Yr25,and Yr44(A:V≈1:3) loci by a single recessive gene.However,avirulence to the remaining 15 resistant loci including Yr1,Yr2,Yr3,Yr4,Yr8,Yr9,Yr17,Yr26(=Yr24),Yr28,Yr29,Yr43,YrSP,Yr27,YrA,and YrExp2,with various avirulence and virulence segregation ratios,is controlled by two genes with different gene effects,indicating complex genetic traits of the parental isolate.Totally,65 dissimilar genotypes detected among progenies using overall molecular markers,by which a linkage map was constructed,with a genetic distance of 441.0 cM.Interestingly,although the parental isolate was avirulent to Yr5,but 17 progenies showed virulence,showing the change of pathogenicity from avirulence to virulence at this resistance locus.It was for the first time to report that progenies with virulence to Yr5 produced sexually from avirulent parental isolate at this resistance locus.To our knowledge,this study offers an insight into inheritance,sexual reproduction and virulence variation of P.striiformis f.sp.tritici in Xinjiang,facilitating us to understand evolution of the rust pathogen in this region and accounting for Xinjiang population distinguished form other inland populations.Additionally,it is necessary to further confirm the roles of sexual reproduction in the emergence of new races and affecting population genetic diversity of P.striiformis f.sp.tritici under natural conditions in Xinjiang.
基金supported by the National Natural Science Foundation of China(Nos.82273845,82304331,and 82360695)Guangxi Natural Science Foundation project(No.2023GXNSFBA026305).
文摘Hyperactivation of DNA repairing pathway is highly associated with the chemosensitivity and chemoresistance of cancer cells.In this manuscript,guided by cascaded one strain many compounds-global natural products social molecular networking(OSMAC-GNPS)strategy,a pair of epimeric environmental-induced metabolites were isolated from Aspergillus sp.EGF 15-0-3.Structurally,sterpiperazines A(1)and B(2)represent the first steroid-based indole alkaloids with unprecedented backbones.Biologically,compound 1 could be identified as a novel tyrosyl-DNA phosphodiesterase 1(Tdp1)inhibitor with a unique mechanism distinct from the reported modulators,and was able to significantly enhance the sensitivity of NCI-H460 cells to the clinic chemotherapeutic drug through inhibiting the DNA repairment and enhanced the DNA damage of cancer cells.
