目的构建金铁锁全长cDNA文库,为进一步开展金铁锁次生代谢合成途径相关基因的研究奠定基础。方法利用Rneasy Plant Mini Kit(Qiagen)试剂盒提取金铁锁根部总RNA,通过SMARTer(switching mechanism at 5'end of RNA transcript)技术...目的构建金铁锁全长cDNA文库,为进一步开展金铁锁次生代谢合成途径相关基因的研究奠定基础。方法利用Rneasy Plant Mini Kit(Qiagen)试剂盒提取金铁锁根部总RNA,通过SMARTer(switching mechanism at 5'end of RNA transcript)技术构建全长cDNA文库,测定分析文库重组率及插入片段大小,对文库进行随机测序并通过Blastx分析后在Genbank中进行同源性检索和功能分析。结果使用SMARTer技术构建的金铁锁cDNA文库的质量较高,在随机挑选的单菌落克隆中都有插入片段,重组率为100%;在15个单克隆中,扩增片段长度在750bp以上的有14个,占93.3%。结论利用SMARTer技术成功构建了金铁锁根部的全长cDNA文库,该文库可用于金铁锁今后功能基因组鉴定、新基因筛选及次生代谢产物生物合成的表达调控研究。展开更多
SUMEC Group Corp. is one of the largest exporters of machinery products in east China＇s Jiangsu Province. Founded in 1978, the company used to be an original equipment manufacturer for foreign big names. Facing falte...SUMEC Group Corp. is one of the largest exporters of machinery products in east China＇s Jiangsu Province. Founded in 1978, the company used to be an original equipment manufacturer for foreign big names. Facing faltering demand and decreasing profit margins after the financial crisis since 2008, the company made a tough decision to change its business model, which later enabled it to transform to an original design manufacturer and finally an original brand manufacturer.展开更多
Intelligent technologies help China upgrade its traditional manufacturingindustry while bringing convenience to people＇s lifeLIKE the countless white-collar workers in China＇s many state-owned enterprises based in B...Intelligent technologies help China upgrade its traditional manufacturingindustry while bringing convenience to people＇s lifeLIKE the countless white-collar workers in China＇s many state-owned enterprises based in Beijing,Zhou Yun is busy from sunrise to sunset,with little time to devote to housework and cooking.But thanks to a China-developed intelligent technology,she now has the comfort of knowing that at the end of her long work day, a full bowl of hotsteamy rice is waiting for her back home.展开更多
FOR Wang Xiuzhen, transplanting rice seedlings all day in the past was tiring work. The paddy fields became a place of pain for the Chinese woman farmer in her 40s, causing her to suffer from endless back problems and...FOR Wang Xiuzhen, transplanting rice seedlings all day in the past was tiring work. The paddy fields became a place of pain for the Chinese woman farmer in her 40s, causing her to suffer from endless back problems and dread each waking day.展开更多
The recent advancements made in the realms of Artificial Intelligence(AI)and Artificial Intelligence of Things(AIoT)have unveiled transformative prospects and opportunities to enhance and optimize the environmental pe...The recent advancements made in the realms of Artificial Intelligence(AI)and Artificial Intelligence of Things(AIoT)have unveiled transformative prospects and opportunities to enhance and optimize the environmental performance and efficiency of smart cities.These strides have,in turn,impacted smart eco-cities,catalyzing ongoing improvements and driving solutions to address complex environmental challenges.This aligns with the visionary concept of smarter eco-cities,an emerging paradigm of urbanism characterized by the seamless integration of advanced technologies and environmental strategies.However,there remains a significant gap in thoroughly understanding this new paradigm and the intricate spectrum of its multifaceted underlying dimensions.To bridge this gap,this study provides a comprehensive systematic review of the burgeoning landscape of smarter eco-cities and their leadingedge AI and AIoT solutions for environmental sustainability.To ensure thoroughness,the study employs a unified evidence synthesis framework integrating aggregative,configurative,and narrative synthesis approaches.At the core of this study lie these subsequent research inquiries:What are the foundational underpinnings of emerging smarter eco-cities,and how do they intricately interrelate,particularly urbanism paradigms,environmental solutions,and data-driven technologies?What are the key drivers and enablers propelling the materialization of smarter eco-cities?What are the primary AI and AIoT solutions that can be harnessed in the development of smarter eco-cities?In what ways do AI and AIoT technologies contribute to fostering environmental sustainability practices,and what potential benefits and opportunities do they offer for smarter eco-cities?What challenges and barriers arise in the implementation of AI and AIoT solutions for the development of smarter eco-cities?The findings significantly deepen and broaden our understanding of both the significant potential of AI and AIoT technologies to enhance sustainable urban development practices,as well as the formidable nature of the challenges they pose.Beyond theoretical enrichment,these findings offer invaluable insights and new perspectives poised to empower policymakers,practitioners,and researchers to advance the integration of eco-urbanism and AI-and AIoT-driven urbanism.Through an insightful exploration of the contemporary urban landscape and the identification of successfully applied AI and AIoT solutions,stakeholders gain the necessary groundwork for making well-informed decisions,implementing effective strategies,and designing policies that prioritize environmental well-being.展开更多
List of China’s top 500 companies shows new trends Profitability,innovation and internationalization—these are some of the focuses of the country’s top companies.Seventy years after the founding of the People’s Re...List of China’s top 500 companies shows new trends Profitability,innovation and internationalization—these are some of the focuses of the country’s top companies.Seventy years after the founding of the People’s Republic of China in 1949,China’s corporate scene has transformed,with market forces playing a dominant role.展开更多
China has started testing its governmentbacked digital legal tender,digital currency and electronic payment(DC/EP),in some regions before it is widely applied to replace paper notes and coins in circulation.
本文标题中的Motherhood即“母性”的意思。具体言之,本文的Motherhood即pregnancy(怀孕)。鼠的试验表明,生育能使脑中的海马状突起大脑侧面脑室壁上的隆起物(主要由灰白质构成,在泛记过程中起主要作用)发生变化。这种变化能够增强思维...本文标题中的Motherhood即“母性”的意思。具体言之,本文的Motherhood即pregnancy(怀孕)。鼠的试验表明,生育能使脑中的海马状突起大脑侧面脑室壁上的隆起物(主要由灰白质构成,在泛记过程中起主要作用)发生变化。这种变化能够增强思维能力,改善记忆,并能预防老年痴呆疾病的发生。且此种变化,随生育次数的增加而增加。文章结尾处,科研人员讲述了自己潜心研究此课题的由来,非常生动感人,且本文遣词造句,文采不俗,值得英语学习者琢磨。如:bathing/translate into这些寻常字眼,在本文用得神采熠熠。此外,文章的末尾句也值得我们再三回味:Nature seems to provide the mother with aboost(本义:增加‘鼓舞)to enable her to care,long term,for the most importantand costly genetic and metabolic(新陈代谢的)investment she will ever make——her offspring.展开更多
https://www. sciencedirect. com/journal/energy-and-buildings/vol/346/suppl/C Volume346, 1November2025[OA](1)Towards energy flexible commercial buildings:Machine learning approaches,implementation aspects,and future re...https://www. sciencedirect. com/journal/energy-and-buildings/vol/346/suppl/C Volume346, 1November2025[OA](1)Towards energy flexible commercial buildings:Machine learning approaches,implementation aspects,and future research directions by M. M. A. L. N. Maheepala,Hangxin Li,Dilan Robert,et al,Article116170Abstract:Commercial buildings encounter considerable challenges in predicting and managing energy flexibility,arising from the complexity of their energy systems and the interdependencies among system components and building thermal mass. Nonetheless,the emergence of “smarter buildings” creates significant opportunities for applying machine learning(ML)techniques in energy flexibility.展开更多
文摘目的构建金铁锁全长cDNA文库,为进一步开展金铁锁次生代谢合成途径相关基因的研究奠定基础。方法利用Rneasy Plant Mini Kit(Qiagen)试剂盒提取金铁锁根部总RNA,通过SMARTer(switching mechanism at 5'end of RNA transcript)技术构建全长cDNA文库,测定分析文库重组率及插入片段大小,对文库进行随机测序并通过Blastx分析后在Genbank中进行同源性检索和功能分析。结果使用SMARTer技术构建的金铁锁cDNA文库的质量较高,在随机挑选的单菌落克隆中都有插入片段,重组率为100%;在15个单克隆中,扩增片段长度在750bp以上的有14个,占93.3%。结论利用SMARTer技术成功构建了金铁锁根部的全长cDNA文库,该文库可用于金铁锁今后功能基因组鉴定、新基因筛选及次生代谢产物生物合成的表达调控研究。
文摘SUMEC Group Corp. is one of the largest exporters of machinery products in east China＇s Jiangsu Province. Founded in 1978, the company used to be an original equipment manufacturer for foreign big names. Facing faltering demand and decreasing profit margins after the financial crisis since 2008, the company made a tough decision to change its business model, which later enabled it to transform to an original design manufacturer and finally an original brand manufacturer.
文摘Intelligent technologies help China upgrade its traditional manufacturingindustry while bringing convenience to people＇s lifeLIKE the countless white-collar workers in China＇s many state-owned enterprises based in Beijing,Zhou Yun is busy from sunrise to sunset,with little time to devote to housework and cooking.But thanks to a China-developed intelligent technology,she now has the comfort of knowing that at the end of her long work day, a full bowl of hotsteamy rice is waiting for her back home.
文摘FOR Wang Xiuzhen, transplanting rice seedlings all day in the past was tiring work. The paddy fields became a place of pain for the Chinese woman farmer in her 40s, causing her to suffer from endless back problems and dread each waking day.
基金funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No.101034260.
文摘The recent advancements made in the realms of Artificial Intelligence(AI)and Artificial Intelligence of Things(AIoT)have unveiled transformative prospects and opportunities to enhance and optimize the environmental performance and efficiency of smart cities.These strides have,in turn,impacted smart eco-cities,catalyzing ongoing improvements and driving solutions to address complex environmental challenges.This aligns with the visionary concept of smarter eco-cities,an emerging paradigm of urbanism characterized by the seamless integration of advanced technologies and environmental strategies.However,there remains a significant gap in thoroughly understanding this new paradigm and the intricate spectrum of its multifaceted underlying dimensions.To bridge this gap,this study provides a comprehensive systematic review of the burgeoning landscape of smarter eco-cities and their leadingedge AI and AIoT solutions for environmental sustainability.To ensure thoroughness,the study employs a unified evidence synthesis framework integrating aggregative,configurative,and narrative synthesis approaches.At the core of this study lie these subsequent research inquiries:What are the foundational underpinnings of emerging smarter eco-cities,and how do they intricately interrelate,particularly urbanism paradigms,environmental solutions,and data-driven technologies?What are the key drivers and enablers propelling the materialization of smarter eco-cities?What are the primary AI and AIoT solutions that can be harnessed in the development of smarter eco-cities?In what ways do AI and AIoT technologies contribute to fostering environmental sustainability practices,and what potential benefits and opportunities do they offer for smarter eco-cities?What challenges and barriers arise in the implementation of AI and AIoT solutions for the development of smarter eco-cities?The findings significantly deepen and broaden our understanding of both the significant potential of AI and AIoT technologies to enhance sustainable urban development practices,as well as the formidable nature of the challenges they pose.Beyond theoretical enrichment,these findings offer invaluable insights and new perspectives poised to empower policymakers,practitioners,and researchers to advance the integration of eco-urbanism and AI-and AIoT-driven urbanism.Through an insightful exploration of the contemporary urban landscape and the identification of successfully applied AI and AIoT solutions,stakeholders gain the necessary groundwork for making well-informed decisions,implementing effective strategies,and designing policies that prioritize environmental well-being.
文摘List of China’s top 500 companies shows new trends Profitability,innovation and internationalization—these are some of the focuses of the country’s top companies.Seventy years after the founding of the People’s Republic of China in 1949,China’s corporate scene has transformed,with market forces playing a dominant role.
文摘China has started testing its governmentbacked digital legal tender,digital currency and electronic payment(DC/EP),in some regions before it is widely applied to replace paper notes and coins in circulation.
文摘本文标题中的Motherhood即“母性”的意思。具体言之,本文的Motherhood即pregnancy(怀孕)。鼠的试验表明,生育能使脑中的海马状突起大脑侧面脑室壁上的隆起物(主要由灰白质构成,在泛记过程中起主要作用)发生变化。这种变化能够增强思维能力,改善记忆,并能预防老年痴呆疾病的发生。且此种变化,随生育次数的增加而增加。文章结尾处,科研人员讲述了自己潜心研究此课题的由来,非常生动感人,且本文遣词造句,文采不俗,值得英语学习者琢磨。如:bathing/translate into这些寻常字眼,在本文用得神采熠熠。此外,文章的末尾句也值得我们再三回味:Nature seems to provide the mother with aboost(本义:增加‘鼓舞)to enable her to care,long term,for the most importantand costly genetic and metabolic(新陈代谢的)investment she will ever make——her offspring.
文摘https://www. sciencedirect. com/journal/energy-and-buildings/vol/346/suppl/C Volume346, 1November2025[OA](1)Towards energy flexible commercial buildings:Machine learning approaches,implementation aspects,and future research directions by M. M. A. L. N. Maheepala,Hangxin Li,Dilan Robert,et al,Article116170Abstract:Commercial buildings encounter considerable challenges in predicting and managing energy flexibility,arising from the complexity of their energy systems and the interdependencies among system components and building thermal mass. Nonetheless,the emergence of “smarter buildings” creates significant opportunities for applying machine learning(ML)techniques in energy flexibility.
