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Maximizing recovery time between knock-out races improves sprint cross-country skiing performance 被引量:1
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作者 Kerry McGawley Coline Van Waerbeke +1 位作者 Karl-Johan Westberg Erik P.Andersson 《Journal of Sport and Health Science》 SCIE 2022年第1期21-29,共9页
Background:In a sprint cross-country(XC)ski competition,the difference in recovery times separating the first and the second semi-final(SF)heats from the final(F)may affect performance.The aim of the current study was... Background:In a sprint cross-country(XC)ski competition,the difference in recovery times separating the first and the second semi-final(SF)heats from the final(F)may affect performance.The aim of the current study was to compare the effects of longer vs.shorter recovery periods prescribed between the 3 knock-out races of a simulated sprint XC ski competition involving a prologue(P),quarter-final(QF),SF,and F.Methods:Eleven well-trained XC ski athletes completed 2 simulated sprint XC ski competitions on a treadmill involving 4×883-m roller-ski bouts at a 4°incline using the gear 3 ski-skating sub-technique.The first 3 bouts were completed at a fixed speed(PFIX,QFFIX,and SFFIX)corresponding to~96%of each individual’s previously determined maximal effort.The final bout was performed as a self-paced sprint time trial(FSTT).Test conditions differed by the time durations prescribed between the QFFIX,SFFIX,and FSTT,which simulated real-world XC ski competition conditions using maximum(MAX-REC)or minimum(MIN-REC)recovery periods.Results:The FSTT was completed 5.4±5.5 s faster(p=0.009)during MAX-REC(179.2±18.1 s)compared to MIN-REC(184.6±20.0 s),and this was linked to a significantly higher power output(p=0.010)and total metabolic rate(p=0.009).The pre FSTT blood lactate(BLa)concentration was significantly lower during MAX-REC compared to MIN-REC(2.5±0.8 mmol/L vs.3.6±1.6 mmol/L,respectively;p=0.027),and the pre-to-post FSTT increase in BLa was greater(8.8±2.1 mmol/L vs.7.1±2.3 mmol/L,respectively;p=0.024).No other differences for MAX-REC vs.MIN-REC reached significance(p>0.05).Conclusion:Performance in a group of well-trained XC skiers is negatively affected when recovery times between sprint heats are minimized which,in competition conditions,would occur when selecting the last QF heat.This result is combined with a higher pre-race BLa concentration and a reduced rise in BLa concentration under shorter recovery conditions.These findings may help inform decision making when XC skiers are faced with selecting a QF heat within a sprint competition. 展开更多
关键词 Energetics Repeated sprints Roller skiing Simulated competition TACTICS
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Differences between Cheeks and Dorsal Hands Skin Properties during Skiing in a Cold and Windy Environment
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作者 Sue Im Jang Jiyei Choi +2 位作者 Jiyeon Han Eun Joo Kim Hae Kwang Lee 《Journal of Cosmetics, Dermatological Sciences and Applications》 2017年第1期27-33,共7页
Background/Purpose: The skin protects against external factors and this skin function can be affected by environmental conditions, including low temperature, low humidity, and high altitude. Therefore, it is important... Background/Purpose: The skin protects against external factors and this skin function can be affected by environmental conditions, including low temperature, low humidity, and high altitude. Therefore, it is important to study the relationship between the environment and the skin. The purpose of this study is to determine how the skin barrier functions change at low temperatures. Methods: This study was performed at a Korean ski resort on January 2015 and evaluated 10 subjects skin condition during skiing. Skin properties were examined at 2 sites (cheeks and dorsal hands) and four time points (indoor;baseline, outdoor;after first skiing and after second skiing, indoor;after taking a shower). Results: The skin hydration on the cheek decreased after the second skiing session and the erythema decreased only after the first skiing while this value on dorsal hand was not changed by skiing. The brightness and yellowness of cheek skin increased and redness decreased therefore, skin color became pale during skiing. The extensibility of cheeks and dorsal hands decreased after skiing. We found positive correlation between skin temperature and redness (r2 = 0.707, p Conclusions: Low temperatures can affect human skin color so that the skin color becomes pale because capillary blood vessels shrink when skin is exposed to cold environment. The cheek skin tends to change easily than dorsal hand during ambient temperature change. 展开更多
关键词 Low TEMPERATURE ENVIRONMENT skiing Hydration SKIN Color Elasticity SKIN TEMPERATURE SKIN Redness
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Establishment and Maintenance of Grass Skiing Resort Turf Based on Climatic Characteristics of Western Sichuan
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作者 LIU Hongxia 《Journal of Landscape Research》 2017年第6期4-6,共3页
In recent years, a new type of leisure sport—grass skiing, has been introduced from abroad. However, there is no standard design system for this type of turf. This paper, based on climatic characteristics of western ... In recent years, a new type of leisure sport—grass skiing, has been introduced from abroad. However, there is no standard design system for this type of turf. This paper, based on climatic characteristics of western Sichuan, introduced the requirements of the construction of grass skiing resort turf in western Sichuan as well as its maintenance management mode, and tried to explore the standard system of turf establishment and maintenance management of grass skiing resort in western Sichuan. 展开更多
关键词 Climatic characteristics of western Sichuan Grass skiing resort Establishment and maintenance
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The Generation and Elimination of Fear Factors in Alpine Skiing Training
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作者 JIANG Anna 《外文科技期刊数据库(文摘版)教育科学》 2021年第7期029-031,共5页
Alpine skiing is a popular sport in the northern region. Every winter, a large number of students sign up for alpine skiing training. There are also various groups of students in alpine skiing training. Some students ... Alpine skiing is a popular sport in the northern region. Every winter, a large number of students sign up for alpine skiing training. There are also various groups of students in alpine skiing training. Some students who lack physical training quality or have poor psychological quality will have fear of alpine skiing training, which leads to their poor performance and affects the training effect. From the coach's point of view, it is necessary to find out the specific factors of students' fear, and to arrange some effective measures to eliminate the college's fear combined with the specific procedures of alpine skiing training, so as to improve the effect of skiing training. 展开更多
关键词 alpine skiing training fear psychology psychological cure
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Comparison and analysis of skiing injuries at ski resorts in Chongli,China and Japan 被引量:2
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作者 Xiao Ma Jiong-Yuan Li +2 位作者 Shi Geru Andd Ying-Fang Ao Yu-Ping Yang 《Chinese Journal of Traumatology》 CAS CSCD 2023年第2期63-67,共5页
Purpose:The rapid development of winter sports requires investigation on injuries in Chongli district,Zhangjiakou city,one of the ski sites of the 2022 Winter Olympics.Careful evaluation is required to observe which i... Purpose:The rapid development of winter sports requires investigation on injuries in Chongli district,Zhangjiakou city,one of the ski sites of the 2022 Winter Olympics.Careful evaluation is required to observe which injuries are caused under what circumstances,and then we can make corresponding preventive measures and recommendations based on the results.Methods:In this retrospective study,the data of injury cases at ski resorts in China(Chongli district)and Japan were analyzed to provide a reference for the ongoing injury prevention at ski resorts.We collected data on injuries at Wanlong and Fulong ski resorts in Chongli district during the 2017–2018 and 2018-2019 ski seasons.We referred to the skiing injury report issued in February 2020 of a nationwide ski safety statistical service–Japan Ski Safety Association.The causes of injury and specific injured body parts were analyzed based on the data of Chinese and Japanese ski resorts.Statistical significance has been calculated using the Chi-square test.Results:During the 2019–2020 ski season in Japanese ski resorts,the number of reported injuries per 10,000 skiers was 0.93,of which 457(17.3%)were over 50 years old,accounting for a large proportion of injuries,meanwhile in Chongli ski resort,the injury rate of skiers aged 50 and over was 7.1%.The knee joint(23.7%at Wanlong ski resort and 28.4%in Japanese ski resorts)was the most injured body part among Chongli and Japanese skiers.Among snowboarders,shoulder joint injury(17.7%in Japanese ski resorts)was the most common,and injury on hands and fingers(16.3%in Wanlong ski resorts)was the most common.Head injury rates are similar in Chongli,China and Japanese ski resorts(8.2%and 8.7%,respectively).Conclusion:Our analysis demonstrated that injury data recorded among young skiers was higher in Chinese ski resorts(Chongli district)than that in Japanese ski resorts,and elderly skiers made up a larger proportion of skiing injuries in Japanese resorts.Thus,according to our research,the protection of knee joints,shoulder joints,and hands and fingers should be taken seriously.It should pay attention to the teaching of ski poles(for finger protection),and use protective devices such as knee pads,helmets,etc. 展开更多
关键词 Ski resorts skiing Sports injury The winter olympics
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Effects of Additional Rifle-Carriage Training on Physiological Markers and Roller-Skiing Performance in Well-Trained Biathletes 被引量:1
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作者 Malin Jonsson Karstrom Kerry McGawley Marko S.Laaksonen 《Journal of Science in Sport and Exercise》 2021年第3期292-302,共11页
Purpose The purpose was to investigate whether an increased amount of training while carrying the rifle affects skiing in well-trained biathletes at submaximal and maximal workloads during a pre-season period lasting ... Purpose The purpose was to investigate whether an increased amount of training while carrying the rifle affects skiing in well-trained biathletes at submaximal and maximal workloads during a pre-season period lasting a minimum of 12 weeks.Methods Seventeen well-trained biathletes(9 females,8 males)were assigned to an intervention(IG,n=10)or control(CG,n=7)group.Before(T1)and after(T2)the training intervention all participants performed,using treadmill roller-skiing,a submaximal test without the rifle on one day and two submaximal workloads and a maximal time trial(TT)with the rifle on a subsequent day.Between T1 and T2 all participants performed a minimum of 12 weeks of normal training,the only difference between groups being that IG performed more of their training sessions carrying the rifle.Results IG performed more training compared to CG(15.4±1.1 vs.11.2±2.6 h/week,P<0.05),including a higher amount of training with the rifle(3.1±0.6 vs.1.1±0.3 h/week,P<0.001).Speed at 4 mmol/L of blood lactate increased significantly for CG from T1 to T2(P=0.028),while only tended to increase for IG(P=0.058).Performance during the TT,VO2max and the aerobic metabolic rate increased significantly from T1 to T2,although the differences disappeared when including the speed at baseline as a covariate.Conclusion According to the present results,increasing training while carrying the rifle by 2 h/week does not appear to improve skiing performance in well-trained biathletes.In addition,physiological markers at submaximal and maximal intensities while carrying the rifle were not affected after the training intervention. 展开更多
关键词 Anaerobic energy contribution Gross efficiency Lactate threshold Nordic skiing Oxygen uptake Training intervention
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Three-Rigid-Body-Particle Modeling and Optimization of Trajectory and Posture for Alpine Skiing
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作者 ZHANG Yijia FEI Qing +3 位作者 YAO Xiaolan SUN Jian ZHANG Yanjun CHEN Zhen 《Journal of Systems Science & Complexity》 SCIE EI CSCD 2024年第2期581-608,共28页
This paper presents a new study on modeling and optimization of trajectory and posture for the super-giant(SG)slalom of alpine skiing.It is the first time that a Three-Rigid-Body-Particle model based on rigorous deriv... This paper presents a new study on modeling and optimization of trajectory and posture for the super-giant(SG)slalom of alpine skiing.It is the first time that a Three-Rigid-Body-Particle model based on rigorous derivations and stability analysis is established to represent skiers trajectory and posture characteristics,as it is more accurate than the single-rigid-body model which is commonly used in existing studies.In addition,the Radau pseudospectral method is applied to solve the trajectory and posture optimization problem in order to obtain better skiing trajectory,skiing posture,and some key kinematic parameters of skiers.Moreover,this paper analyzes the effects of different body types,minimum turning radii,and flexor and extensor strength of knees and hip joint on skiing performance.Finally,based on the findings of the study,some advice about how to improve the performance of the SG slalom in view of science and technology is given to skiers and coaches for reference. 展开更多
关键词 Alpine skiing modeling posture optimization Three-Rigid-Body-Particle model trajectory optimization
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The Influence of a Fast Start Versus a Conservative Start Pacing Strategy on Sprint Roller Skiing Performance in Highly Trained Female Junior Skiers
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作者 Thomas Losnegard Even Dahlen Granrud +3 位作者 Ola Kristoffer Tosterud Kasper Kjeldsen Øyvind Olstad Jan Kocbach 《Journal of Science in Sport and Exercise》 CSCD 2024年第2期146-154,共9页
Purpose Pacing profiles differ substantially in endurance sports with short competition duration(2-4 min).Herein,we investigated the effect of a fast start(FS)versus a conservative start(CS)pacing strategy on sprint t... Purpose Pacing profiles differ substantially in endurance sports with short competition duration(2-4 min).Herein,we investigated the effect of a fast start(FS)versus a conservative start(CS)pacing strategy on sprint time-trial performance in competitive junior cross-country skiers.Methods Sixteen females(~17 years old)performed two individual self-paced 1.4 km time-trials(TT)in free technique on roller skis separated by 40 min of recovery.Skiers were instructed to perform a FS and CS pacing strategy the first~400-m(~1 min)in a randomized order.Split and finish time was determined using radio-based photocells,while speed characteris-tics were collected with GNSS/IMU sensors.1-10 Rate of perceived exertion(RPE)was collected retrospectively for 3 points during the TT.Based on mean FS and CS performance,skiers were divided into a Low-(n=8)and High performers(n=8).Results Overall,the FS strategy induced a faster~400-m time(56.0±3.8 s)than CS(58.7±3.4 s)(P<0.05),but no differ-ences in finish times were found between strategies for the group as a whole(FS:213.3±12.0 s;CS,212.9±10.3 s,P=0.68).However,High performers skied faster with FS than CS(203.4±7.4 s vs.205.1±7.1 s;0.8%±0.7%,P<0.05),whereas the Low performers skied faster with CS than FS(220.7±6.1 s vs.223.1±5.0 s;1.1%±1.0%,P<0.05).Independent of performance level,summated RPE during the TT was higher with FS versus CS(23±2 vs.22±2,P<0.05).Conclusion Optimal pacing strategy for TT sprint skiing appears dependent of performance level.A“fast start”strategy was accompanied by higher discomfort during the time-trial. 展开更多
关键词 Cross-country skiing GNSS Intermittent exercise PERFORMANCE Rate of perceived exertion
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Performance and Micro-Pacing Strategies in Biathlon Skiing
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作者 Craig A.Staunton Malin Jonsson Kårström +1 位作者 Glenn Björklund Marko S.Laaksonen 《Journal of Science in Sport and Exercise》 CSCD 2024年第4期324-331,共8页
Purpose To examine the micro-pacing(within-lap)strategies during biathlon skiing with and without the biathlon rifle.Methods Twenty biathletes(7 women,13 men)performed two cross-country skiing time-trials on a≈2300 m... Purpose To examine the micro-pacing(within-lap)strategies during biathlon skiing with and without the biathlon rifle.Methods Twenty biathletes(7 women,13 men)performed two cross-country skiing time-trials on a≈2300 m course,once with and once without the biathlon rifle.During time-trials,biathletes wore a sensor that recorded position,distance and skiing-speed.A trajectory correction and statistical parametric mapping procedure determined the course positions(clusters)where instantaneous skiing-speed was significantly related to time-trial performance.The time differences between the fastest and slowest skier in these clusters were calculated.Results The fastest biathletes skied with greater instantaneous speeds in specific clusters,which included both uphill and downhill sections.The clusters represented time gains for the fastest skier over the slowest skier of between 16.1 and 25.8 s for the women and between 18.9 and 21.9 s for the men.The largest time gains between the fastest and slowest biathletes were observed in a downhill section that was preceded by a 180°turn,where time gains were between 2.9 and 4.1 s in clusters of between just 12 to 62 m.In biathlon skiing with-rifle,there were more clusters that were not present during without-rifle.When skiing with-rifle,there were additional clusters in the uphill sections that represented time gains of 5.2 s and 2.3 s for the women and men,respectively.Conclusions Statistical parametric mapping can be used in biathlon to provide pacing and performance feedback to athletes and coaches. 展开更多
关键词 Cross country skiing GNSS sensor Performance analysis Statistical parametric mapping Winter sport
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In-depth Ice and Snow 6-Day Trip in Changbai Mountain
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《China & The World Cultural Exchange》 2025年第2期28-31,共4页
This is an in-depth journey to experience the ice and snow of Changbai Mountain.In these few days,you will explore Changbai Mountain and enjoy powder skiing;gallop on the ski trail;watch the stunning wonders of snow r... This is an in-depth journey to experience the ice and snow of Changbai Mountain.In these few days,you will explore Changbai Mountain and enjoy powder skiing;gallop on the ski trail;watch the stunning wonders of snow rime on thousands of trees;conquer the ice and snow wilderness on a snowmobile and start an in depth magical mystery tour in lilin Province. 展开更多
关键词 Changbai Mountain ice snow powder skiinggallop snow rime changbai mountain powder skiing ICE SNOW
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The 9th Asian Winter Games
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作者 常聪莹 张超(指导) 《中学生英语》 2025年第15期7-7,共1页
The 9th Asian Winter Games were successfully held in Harbin,Heilongjiang Province,China,from February 7 to February 14,2025.This exciting event brought together athletes from all over Asia.They showed their great skil... The 9th Asian Winter Games were successfully held in Harbin,Heilongjiang Province,China,from February 7 to February 14,2025.This exciting event brought together athletes from all over Asia.They showed their great skills and strong will in different kinds of winter sports,such as skiing,skating and ice hockey. 展开更多
关键词 SKATING Asian Winter Games Heilongjiang province skiing winter sportssuch winter sports China HARBIN
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Ecological security evaluation and early warning of ski tourism destinations
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作者 TANG Chengcai HAN Ying JIANG Ling 《Journal of Geographical Sciences》 2025年第9期1953-1978,共26页
To address the contradiction between the rapid development of ski tourism and effective protection of the ecological environment,this study constructed the DPSIR-EES(Drive-Pressures-State-Impact-Response-Environment-E... To address the contradiction between the rapid development of ski tourism and effective protection of the ecological environment,this study constructed the DPSIR-EES(Drive-Pressures-State-Impact-Response-Environment-Economy-Society)model and Ski Tourism Destination Ecological Security System(STDESS)framework system.They form an integrated methodology system based on the“entropy weighting-hierarchical analysis-gray correlation projection”composite weighting method that can be used to clarify the intrinsic mechanism of ecological security in ski tourism destinations.Taking Chongli as a case study,this study evaluated the evolution of its ecological security from 1995 to 2023,predicted the ecological security early warning levels from 2024 to 2050,and analyzed the mechanism of influences on regional ecological security.The findings indicate that the ecological security of ski tourism destinations shows a significant“stepped leap–dynamic equilibrium”evolutionary path.The dynamic response mechanism of the subsystems is characterized by significant heterogeneity.The ecological security early warning system revealed the temporal and sequential differentiation of risk transmission.The factors influencing ecological security show the significant dual dominance of policy and climate.This paper enhances the applicability of ecological security systems within ski tourism contexts by analyzing their evolutionary characteristics,predicted future changes and impact factors,and it provides an effective case study for ecological improvement. 展开更多
关键词 ecological security ski tourism tourism destination sustainable development Beijing Winter Olympics
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广义Sierpiński网络拓扑指数信息熵的研究
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作者 刘家保 司家东 《安徽建筑大学学报》 2025年第5期65-70,83,共7页
本文对广义Sierpiński网络边基于度的划分,运用拓扑指数及信息熵的定义,得到十类拓扑指数信息熵的精确表达,分析其拓扑指数信息熵的特性。结果表明:十类拓扑指数信息熵均随网络演化呈上升趋势,表明网络扩展过程中拓扑复杂性显著增加。... 本文对广义Sierpiński网络边基于度的划分,运用拓扑指数及信息熵的定义,得到十类拓扑指数信息熵的精确表达,分析其拓扑指数信息熵的特性。结果表明:十类拓扑指数信息熵均随网络演化呈上升趋势,表明网络扩展过程中拓扑复杂性显著增加。研究结果为验证分形网络的确定性生成机制,建模自相似系统提供理论支持。 展开更多
关键词 Sierpiński网络 拓扑指数 信息熵
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自由式滑雪空中技巧项目女子技术发展趋势的研究 被引量:1
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作者 陈伟光 杨李丽 《哈尔滨体育学院学报》 2004年第3期86-87,共2页
自由式滑雪空中技巧项目是我国近年来发展起来的项目 ,在国家冬季项目管理中心的支持下 ,特别是女子项目在我国得到了极快发展。本课题根据第 19届冬奥会本项目比赛的情况 ,展望第 2 0届冬奥会女子空中技术比赛可能使用的最难动作问题 。
关键词 FREESTYLE skiing moguls aerials
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Biomechanical analysis of the "running" vs. "conventional" diagonal stride uphill techniques as performed by elite cross-country skiers 被引量:1
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作者 Barbara Pellegrini Chiara Zoppirolli +3 位作者 Federico Stella Lorenzo Bortolan Hans-Christer Holmberg Federico Schena 《Journal of Sport and Health Science》 SCIE 2022年第1期30-39,共10页
Purpose:This study aimed to compare biomechanical aspects of a novel"running"diagonal stride(DSRUN)with"conventional"diagonal stride(DSCONV)skiing techniques performed at high speed.Methods:Ten eli... Purpose:This study aimed to compare biomechanical aspects of a novel"running"diagonal stride(DSRUN)with"conventional"diagonal stride(DSCONV)skiing techniques performed at high speed.Methods:Ten elite Italian male junior cross-country skiers skied on a treadmill at 10 km/h and at a 10°incline utilizing both variants of the diagonal stride technique.The 3-dimensional kinematics of the body,poles,and roller skis;the force exerted through the poles and foot plantar surfaces;and the angular motion of the leg joints were determined.Results:Compared to DSCONV,DSRUN demonstrated shorter cycle times(1.05±0.05 s vs.0.75±0.03 s(mean±SD),p<0.001)due to a shorter rolling phase(0.40±0.04 s vs.0.09±0.04 s,p<0.001);greater force applied perpendicularly to the roller skis when they had stopped rolling forward(413±190 N vs.890±170 N,p<0.001),with peak force being attained earlier;prolonged knee extension,with a greater range of motion during the roller ski-stop phase(28°±40 vs.16°±3°,p=0.00014);and more pronounced hip and knee flexion during most of the forward leg swing.The mechanical work performed against friction during rolling was significantly less with DSRUN than with DSCONV(0.04±0.01 J/m/kg vs.0.10±0.02 J/m/kg,p<0.001).Conclusion:Our findings demonstrate that DSRUN is characterize by more rapid propulsion,earlier leg extension,and a greater range of motion of knee joint extension than DSCONV-Further investigations,preferably on snow,should reveal whether DSRUN results in higher acceleration and/or higher peak speed. 展开更多
关键词 Classical skiing Kinetics Roller skiing
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清胰化积方下调Ski表达抗胰腺癌生长实验研究 被引量:9
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作者 王鹏 刘鲁明 +6 位作者 陈震 孟志强 林钧华 周振华 陈颢 王琨 沈晔华 《中国中西医结合杂志》 CAS CSCD 北大核心 2010年第9期942-945,共4页
目的探讨清胰化积方(Qingyi Huaji Formula,QYHJ)是否通过下调Ski表达抑制胰腺肿瘤的生长。方法采用Real-time PCR和Western blot对QYHJ治疗后21天人胰腺癌裸小鼠皮下移植瘤Ski mRNA和蛋白表达进行检测;通过慢病毒介导的RNA干扰技术建立... 目的探讨清胰化积方(Qingyi Huaji Formula,QYHJ)是否通过下调Ski表达抑制胰腺肿瘤的生长。方法采用Real-time PCR和Western blot对QYHJ治疗后21天人胰腺癌裸小鼠皮下移植瘤Ski mRNA和蛋白表达进行检测;通过慢病毒介导的RNA干扰技术建立Ski低表达人胰腺癌细胞系,并通过建立裸小鼠皮下移植瘤,评价Ski不同表达肿瘤对QYHJ治疗反应性是否存在差异。结果 QYHJ治疗后,人胰腺癌SW1990皮下移植瘤Ski mRNA和蛋白表达与未用QYHJ治疗组比较分别下降了39.6%和41.3%(均P<0.05);建立了Ski低表达SW1990细胞系(SW1990/Ski RNAi)和阴性干扰细胞系(SW1990/con RNAi),其Ski mRNA和蛋白表达量分别是亲代细胞的105%、123%和46%、30%(均P<0.05);SW1990细胞和SW1990/con RNAi细胞皮下移植瘤应用QYHJ治疗后其瘤重抑制率分别为29.6%和32.2%,但SW1990/RNAi细胞组其瘤重抑制率为16.0%~17.8%(均P<0.05)。结论 Ski下调增加了人胰腺癌细胞对QYHJ治疗的敏感性,Ski为QYHJ治疗胰腺癌一个重要作用靶点,其不同表达介导了胰腺癌细胞对QYHJ治疗的反应性差异。 展开更多
关键词 清胰化积方 胰腺癌 Ski癌基因 RNA干扰
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Ski在大鼠活化星形胶质细胞中表达的变化 被引量:7
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作者 赵鑫 寇江力 +6 位作者 郭永强 蒲彦川 周开升 南伟 汪静 伍亚民 张海鸿 《中国病理生理杂志》 CAS CSCD 北大核心 2017年第6期968-974,共7页
目的:探究Ski在大鼠正常及活化星形胶质细胞中的表达及随时间变化的规律。方法:提取大鼠大脑皮质,分离星形胶质细胞,体外培养。采用LPS刺激和细胞划痕损伤2种方法活化星形胶质细胞,均采用Western blot法检测各个时点2种活化星形胶质细胞... 目的:探究Ski在大鼠正常及活化星形胶质细胞中的表达及随时间变化的规律。方法:提取大鼠大脑皮质,分离星形胶质细胞,体外培养。采用LPS刺激和细胞划痕损伤2种方法活化星形胶质细胞,均采用Western blot法检测各个时点2种活化星形胶质细胞中Ski和胶质纤维酸性蛋白(GFAP)蛋白表达情况,利用间接免疫荧光法检测Ski蛋白在星形胶质细胞中的表达定位。结果:GFAP蛋白在星形胶质细胞中固有表达,LPS活化和划痕损伤后表达均上调。Ski在正常星形胶质细胞中呈低表达状态,1 mg/L LPS活化星形胶质细胞后,Ski表达开始增加,4 d时表达量最高(P<0.05),6 d时开始下降,但仍高于未活化组;细胞划痕损伤后Ski蛋白表达情况与上述情况高度一致。Ski在正常及LPS活化6 d星形胶质细胞中表达主要集中在胞核,LPS活化后2和4 d时在胞质中出现明确的Ski表达。结论:Ski蛋白表达于星形胶质细胞,并在活化星形胶质细胞中表达上调。由此,我们推测Ski蛋白可能调控星形胶质细胞的活化、增殖等过程。 展开更多
关键词 SKI 胶质纤维酸性蛋白 星形胶质细胞 胶质瘢痕
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沉默ski基因对大鼠星形胶质细胞迁移的影响 被引量:7
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作者 赵鑫 王兴文 +4 位作者 寇江力 李忠浩 郭永强 伍亚民 张海鸿 《中国康复理论与实践》 CSCD 北大核心 2017年第8期905-911,共7页
目的研究ski基因在大鼠星形胶质细胞迁移过程中的作用。方法分离大鼠脑皮质星形胶质细胞,体外培养。合成ski基因和阴性对照小干扰片段。设置ski-siRNA转染组、阴性对照组和空白对照组。采用脂质体法将特异性针对ski基因的siRNA和阴性对... 目的研究ski基因在大鼠星形胶质细胞迁移过程中的作用。方法分离大鼠脑皮质星形胶质细胞,体外培养。合成ski基因和阴性对照小干扰片段。设置ski-siRNA转染组、阴性对照组和空白对照组。采用脂质体法将特异性针对ski基因的siRNA和阴性对照siRNA转入大鼠星形胶质细胞。采用Western blotting检测各组ski蛋白的表达水平;采用Transwell细胞迁移实验和细胞划痕实验检测ski-siRNA转染后星形胶质细胞迁移能力的变化。结果与空白对照组和阴性对照组比较,ski-siRNA转染组细胞内ski蛋白的相对表达水平显著降低(F=132.957,P<0.001)。Transwell细胞迁移实验发现,ski-siRNA转染组每孔迁移细胞数少于空白对照组和阴性对照组(F>47.197,P<0.05)。细胞划痕实验显示,转染组细胞1~5 d的划痕愈合率均明显低于对照组(F>69.187,P<0.01)。结论沉默ski基因能显著抑制星形胶质细胞的迁移能力,ski可能参与星形胶质细胞的转移过程,进而调控胶质瘢痕的形成。 展开更多
关键词 SKI RNA干扰 星形胶质细胞 Transwell细胞迁移实验 划痕实验 大鼠
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SphK1抑制剂SKI Ⅱ抑制肝癌HepG2细胞周期及细胞侵袭 被引量:3
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作者 张彩霞 刘虹 +2 位作者 宫玉艳 何红伟 邵荣光 《药学学报》 CAS CSCD 北大核心 2014年第2期204-208,共5页
鞘氨醇激酶1(sphingosine kinase 1,SphK1)在调控细胞基本生物学功能方面发挥重要作用,本研究考察了SphK1抑制剂SKI II对肝癌细胞HepG2周期及侵袭的影响。采用SRB方法检测细胞存活率,流式细胞仪检测细胞周期分布,Matrigel-Transwell方... 鞘氨醇激酶1(sphingosine kinase 1,SphK1)在调控细胞基本生物学功能方面发挥重要作用,本研究考察了SphK1抑制剂SKI II对肝癌细胞HepG2周期及侵袭的影响。采用SRB方法检测细胞存活率,流式细胞仪检测细胞周期分布,Matrigel-Transwell方法检测细胞侵袭能力,Western blotting检测蛋白表达变化。结果表明,SKI II能够抑制HepG2细胞的存活,诱导HepG2细胞发生G1期阻滞以及抑制HepG2细胞的侵袭;SKI II能降低细胞G1期相关蛋白CDK2、CDK4以及Cdc2的表达水平,同时降低与细胞侵袭相关的MMP2和MMP9的蛋白水平。结果提示,SphK1抑制剂SKI II能引起细胞G1期阻滞及降低细胞侵袭能力,提示SphK1可能成为肝癌治疗的一个潜在靶点。 展开更多
关键词 SphK1 SKI II 肝癌 HEPG2 细胞周期 侵袭
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掌叶半夏提取物的有效部位不同给药途径对人宫颈癌细胞株CaSki裸小鼠移植瘤的抑制作用 被引量:11
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作者 张明星 李桂玲 徐丛剑 《中华中医药杂志》 CAS CSCD 北大核心 2015年第1期199-202,共4页
目的:观察不同给药途径下掌叶半夏提取物的有效部位(PE)对Ca Ski裸小鼠皮下移植瘤的抑制作用,以探索PE的体内最佳给药途径。方法:建立Ca Ski裸小鼠皮下移植瘤模型,待移植瘤体积增至大约100mm3,将裸小鼠随机分为阴性对照组、灌胃组、腹... 目的:观察不同给药途径下掌叶半夏提取物的有效部位(PE)对Ca Ski裸小鼠皮下移植瘤的抑制作用,以探索PE的体内最佳给药途径。方法:建立Ca Ski裸小鼠皮下移植瘤模型,待移植瘤体积增至大约100mm3,将裸小鼠随机分为阴性对照组、灌胃组、腹腔注射组、皮下注射组、顺铂阳性对照组。测量肿瘤径线,计算肿瘤体积并描绘肿瘤生长曲线。药物处理21d,第22天颈椎脱臼处死裸小鼠,称取瘤重,计算抑瘤率。HE染色观察移植瘤组织形态,免疫组化检测移植瘤组织中Ki-67的表达情况。结果:灌胃组、腹腔注射组、顺铂阳性对照组裸小鼠移植瘤重量明显小于阴性对照组和皮下注射组(P<0.05),抑瘤率分别为38.71%,31.86%,45.92%。且灌胃组、腹腔注射组移植瘤组织Ki-67表达明显低于阴性对照组和皮下注射组(P<0.05)。结论:PE能够抑制Ca Ski裸小鼠皮下移植瘤的生长,其体内实验的最佳给药途径可以是灌胃或者腹腔注射,推荐实验中采取灌胃方式。抑制Ki-67的表达可能是PE体内抑瘤作用机制之一。 展开更多
关键词 掌叶半夏提取物的有效部位 宫颈癌 Ca Ski细胞株 移植瘤 裸小鼠 Ki-67
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