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姜黄素对S.aureus引起的奶牛乳腺上皮细胞自噬、炎症和凋亡的影响 被引量:1
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作者 张亮 《现代畜牧科技》 2025年第3期59-63,共5页
拟探究姜黄素在金黄色葡萄球菌(Staphylococcus aureus,S.aureus)感染的奶牛乳腺上皮细胞生理中的作用。采用免疫荧光和蛋白质免疫印迹(Western blotting,WB)法,检测姜黄素对S.aureus感染后奶牛乳腺上皮细胞中细胞自噬、炎性反应和细胞... 拟探究姜黄素在金黄色葡萄球菌(Staphylococcus aureus,S.aureus)感染的奶牛乳腺上皮细胞生理中的作用。采用免疫荧光和蛋白质免疫印迹(Western blotting,WB)法,检测姜黄素对S.aureus感染后奶牛乳腺上皮细胞中细胞自噬、炎性反应和细胞凋亡的影响。结果显示,S.aureus MOI为8∶1,37℃下孵育2 h可有效的使S.aureus侵入乳腺上皮细胞,并且S.aureus能够阻止自噬泡的降解;使用姜黄素处理后,胞内自噬通量增加,主要表现在胞内Beclin-1和LC3水平上升,P62水平降低;使用姜黄素处理后,胞内促炎性因子IL-1β水平降低,抑炎性因子IL-10水平上升;与此同时使用姜黄素能够有效缓解S.aureus引起的细胞凋亡水平升高。研究结果表明,姜黄素对S.aureus引起的奶牛乳腺上皮细胞炎症有治疗作用。 展开更多
关键词 姜黄素 s.aureus 自噬 炎性反应 凋亡
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原料奶贮存和运输过程中S.aureus的暴露评估 被引量:4
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作者 许晓曦 闫军 张书义 《中国乳品工业》 CAS 北大核心 2010年第7期54-58,共5页
以哈尔滨市地区为例,运用概率评估方法研究原料奶中金黄色葡萄球菌(S.aureus)的暴露程度,进而推断其肠毒素产生的可能性。整个暴露评估模型的建模方法采用概率评估法。模型采用Monte Carlo模拟技术,通过风险分析软件@RISK4.5对榨乳后不... 以哈尔滨市地区为例,运用概率评估方法研究原料奶中金黄色葡萄球菌(S.aureus)的暴露程度,进而推断其肠毒素产生的可能性。整个暴露评估模型的建模方法采用概率评估法。模型采用Monte Carlo模拟技术,通过风险分析软件@RISK4.5对榨乳后不同冷却方式、贮存方式、运输方式等可能对乳中S.aureus造成生物性危害的可能性进行了评估。结果表明,个体奶户采用冷水降温方式贮存的牛乳中S.aureus可能引发的安全性风险最大,需严格控制贮存温度和时间,防止S.aureus在原料乳中的迅速生长繁殖及产毒,进而从源头上为乳及其制品的安全生产提供保障。 展开更多
关键词 暴露评估 金黄色葡萄球菌(s.aureus) 原料奶 贮存 运输
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CpG-ODN对S.aureus诱导的山羊实验性乳腺炎乳腺的保护作用研究 被引量:1
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作者 朱于敏 邹思湘 +2 位作者 李震 苗晋锋 邓月娥 《中国预防兽医学报》 CAS CSCD 北大核心 2008年第8期651-655,共5页
选择同处于泌乳初期的睢宁白山羊6头,于右乳区经过乳导管灌注10μg/kgCpG-ODN,左乳区则灌入等体积的灭菌100μL0.01mol/LpH7.2磷酸盐缓冲液(PBS)作为对照,灌注后第3d按同等剂量进行二次灌注,二次灌注后第2d,分别于左右乳区经乳导管灌注3... 选择同处于泌乳初期的睢宁白山羊6头,于右乳区经过乳导管灌注10μg/kgCpG-ODN,左乳区则灌入等体积的灭菌100μL0.01mol/LpH7.2磷酸盐缓冲液(PBS)作为对照,灌注后第3d按同等剂量进行二次灌注,二次灌注后第2d,分别于左右乳区经乳导管灌注3mL(2×109CFU/mL)金黄色葡萄球菌(S.aureus),于灌注细菌前(0h),灌注后8h,16h,24h,48h和72h分别收集左右乳区乳汁进行检测。临床症状观察显示,乳腺内灌注3mL(2×109CFU/mL)的S.aureus能迅速诱导山羊典型的急性乳腺炎症状。组织学观察显示感染S.aureus后72h乳腺腺泡内仍有嗜中性粒细胞(PMN)浸润,但实验乳区明显减少。乳汁S.aureus数同在感染后24h上升至最高,CpG-ODN能显著降低各个时间点乳汁细菌数。乳汁白细胞介素-6(IL-6)水平同在感染后24h上升至最高,CpG-ODN能显著提高感染后24h乳汁IL-6水平。对照和CpG-ODN处理乳区乳汁肿瘤坏死因子-α(TNF-α)水平分别在感染后24h和16h上升至最高,其中在感染后24h实验乳区比对照下降40.63%(P<0.05)。乳汁N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)水平同在感染后16h达最高(P<0.01),CpG-ODN能显著提高感染后8h乳汁NAGase水平。上述结果表明CpG-ODN可通过提高乳汁IL-6水平、加速并促进乳汁TNF-α的释放,从而减少了乳汁中S.aureus数量,减轻了炎症介质对细胞的损伤,对缩短炎症过程也有一定的作用,实验结果证实了CpG-ODN对S.aureus感染诱发的山羊乳腺炎的乳腺有保护作用。 展开更多
关键词 CpG—ODN 山羊 乳腺炎 s.aureus
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U.S.Artificial Intelligence Strategy:Internal and External Mechanisms,Challenges and Evolutionary Trends
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作者 Cai Cuihong 《Contemporary World》 2025年第3期19-24,共6页
Artificial Intelligence(AI),as a strategic technology leading the new round of scientific and technological revolution and industrial transformation,is reshaping the global power dynamics.Major countries are accelerat... Artificial Intelligence(AI),as a strategic technology leading the new round of scientific and technological revolution and industrial transformation,is reshaping the global power dynamics.Major countries are accelerating their strategic deployment around technological innovation,standard-setting,and rule-building,making technology increasingly intertwined with geopolitics.Since the U.S.released Preparing for the Future of Artificial Intelligence in 2016,it has integrated AI into its national strategic system and continuously upgraded it,aiming to maintain its dominance in the global competition.Meanwhile,the internal and external tensions of the U.S.AI strategy have become increasingly prominent,profoundly influencing its global strategy and future trajectory. 展开更多
关键词 artificial intelligence ai strategic system preparing future artificial intelligence scientific technological revolution internal mechanisms U S AI strategy CHALLENGES industrial transformationis
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秋水仙素诱导马铃薯野生种S.acaule多倍体的初步研究 被引量:6
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作者 张艳萍 蒲秀琴 《中国种业》 2018年第6期66-68,共3页
马铃薯野生种需通过人工诱导染色体加倍后才能与栽培种进行杂交。本试验以马铃薯野生种S.acaule无菌苗带腋芽茎段为材料,采用浸泡法和混合培养法研究了秋水仙素诱导马铃薯野生种S.acaule产生多倍体的诱导效果。结果表明:在继代增殖培养... 马铃薯野生种需通过人工诱导染色体加倍后才能与栽培种进行杂交。本试验以马铃薯野生种S.acaule无菌苗带腋芽茎段为材料,采用浸泡法和混合培养法研究了秋水仙素诱导马铃薯野生种S.acaule产生多倍体的诱导效果。结果表明:在继代增殖培养基中添加浓度为0.3%的秋水仙素,共同培养时间为14d时获得诱导变异率最大,为31.6%;获得的87株变异株有2株为多倍体植株。 展开更多
关键词 马铃薯野生种s.acaule 秋水仙素 茎段 多倍体
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L.paracasei HD1-7与S.albulus1-25原生质体融合选育产天然防腐剂的优良菌株
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作者 雷虹 张铁丹 +5 位作者 金忠斌 庄海霁 何欣 张基亮 平文祥 周东坡 《食品工业科技》 CAS CSCD 北大核心 2011年第1期146-150,155,共6页
为获得更具优良应用特性的微生物源天然防腐剂,采用原生质体融合技术,以Lactobacillus paracasei HD1-7和Streptomyces albulus1-25为亲株选育优良菌株,研究了L.paracasei HD1-7和S.albulus1-25原生质体制备与再生条件,通过表型、抑菌... 为获得更具优良应用特性的微生物源天然防腐剂,采用原生质体融合技术,以Lactobacillus paracasei HD1-7和Streptomyces albulus1-25为亲株选育优良菌株,研究了L.paracasei HD1-7和S.albulus1-25原生质体制备与再生条件,通过表型、抑菌谱、抗药性和总DNA含量比较等手段筛选鉴定融合子,最终选育出3株以L.paracasei HD1-7为受体、获得来自S.albulus1-25的抑酵母菌基因的融合子。 展开更多
关键词 L.paracasei HD1-7 s.albulus1-25 天然防腐剂 原生质体制备 融合子鉴定
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从《漂亮的三丫头》与“Miss U.S.A.”原、译文的交叉比较看口述实录文学的特点
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作者 魏芳 《北京印刷学院学报》 2011年第3期50-53,共4页
口述实录文学作品主要通过口语形式表达,属于谈话语体,其特点是句子结构简洁,语言通俗流畅、形象生动,有强烈的生活气息。《漂亮的三丫头》和"Miss U.S.A."是题材风格非常接近,极有可比性的两篇口述实录文学作品。通过对《漂... 口述实录文学作品主要通过口语形式表达,属于谈话语体,其特点是句子结构简洁,语言通俗流畅、形象生动,有强烈的生活气息。《漂亮的三丫头》和"Miss U.S.A."是题材风格非常接近,极有可比性的两篇口述实录文学作品。通过对《漂亮的三丫头》与"Miss U.S.A."的原、译文作交叉对照评析,能很好地检验各自译文在语体风格上对原文的忠实程度。 展开更多
关键词 MISS U.s.a 漂亮的三丫头 翻译 语体风格
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灾难的非虚构书写——评S.A.阿列克谢耶维奇的《我还是想你,妈妈》与杨显惠的《定西孤儿院纪事》 被引量:1
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作者 江河 《写作》 2016年第8期48-54,共7页
S.A.阿列克谢耶维奇的《我还是想你,妈妈》与杨显惠的《定西孤儿院纪事》都是以儿童为主人公的非虚构文学。非虚构文学面对灾难性的历史题材有更能打动人的真实的力量。这种打动人的力量一方面来至于非虚构本身,另一方面来自于作家对所... S.A.阿列克谢耶维奇的《我还是想你,妈妈》与杨显惠的《定西孤儿院纪事》都是以儿童为主人公的非虚构文学。非虚构文学面对灾难性的历史题材有更能打动人的真实的力量。这种打动人的力量一方面来至于非虚构本身,另一方面来自于作家对所叙述人物和事件的情感注入。对儿童的关注,在阿氏是刻意的主观选择,在杨显惠是与历史事件的客观遇合。 展开更多
关键词 s.a.阿列克谢耶维奇 杨显惠 灾难 非虚构 人性 儿童视角
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Insight into microbiologically induced corrosion performance of magnesium in tryptic soy broth with S.aureus and E.coli 被引量:1
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作者 Tao Liang Huanhuan Zhang +1 位作者 Haobo Pan Ying Zhao 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2022年第20期221-231,共11页
In this research,microbiologically induced corrosion(MIC)performance of magnesium in tryptic soy broth with S.aureus or E.coli were systematically explored.The results showed that magnesium in biotic solution presente... In this research,microbiologically induced corrosion(MIC)performance of magnesium in tryptic soy broth with S.aureus or E.coli were systematically explored.The results showed that magnesium in biotic solution presented higher mass loss,thicker degradation product layer and more severe pitting corrosion compared to abiotic solution after 24 h of immersion.Electrochemical measurements including EIS spectra and polarization curve further suggested that the corrosion rate of Mg in biotic solution increased during the early stage owing to the decrease of p H,which was originated from the consumption of glucose and the resulting accumulation of acidic metabolites.However,corrosion rate subsequently decreased because of the reduction of acidic metabolites and production of alkaline compound as well as the corrosion protection provided by the corrosion product and biofilm.Moreover,the corrosion rate of magnesium in high concentration of S.aureus and E.coli solution was larger than that in low concentration of S.aureus and E.coli solution,respectively during the initial stage,but subsequently presented a reverse variation.In addition,more severe corrosion of magnesium was observed in S.aureus solution in comparison with E.coli solution.These results suggested that the MIC performance of magnesium was closely related to the concentration and species of bacteria. 展开更多
关键词 MAGNESIUM s.aureus E.COLI Microbiologically induced corrosion
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Effect of Ciprofloxacin on S.aureus and E.coli Growth in Presence of Vitamin C Using Cup Cut Diffusion Method 被引量:1
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作者 Suhera M. Aburawi Bassma M. Doro Ebtisam A. Awad 《Journal of Pharmacy and Pharmacology》 2019年第8期473-484,共12页
Ciprofloxacin is a second-generation of fluoroquinolone,broad-spectrum antibiotic with bactericidal activity against Gram-positive and Gram-negative organisms.It is one of the most widely used antibiotics,because of i... Ciprofloxacin is a second-generation of fluoroquinolone,broad-spectrum antibiotic with bactericidal activity against Gram-positive and Gram-negative organisms.It is one of the most widely used antibiotics,because of its efficacy,safety,and relatively low cost.Ascorbic acid(vitamin C)is water-soluble monosaccharide antioxidant;it is essentially required by the body for its various biochemical and physiological processes.S.aureus is Gram-positive cocci;widely distributed in the environment,it is a member of the normal flora of the body.S.aureus is not always pathogenic;it is a common cause of skin infections including abscesses,respiratory infections such as sinusitis,and food poisoning.E.coli is Gram-negative bacteria,found in the environment,foods,and intestines.Most E.coli strains are harmless;it is part of the normal microbiota of the gut.However,some serotypes of E.coli cause serious food poisoning in their hosts;it can cause diarrhea,while others cause urinary tract infections,respiratory illness and pneumonia,and other illnesses.Method:Cup cut diffusion method was applied.Experiment I:is carried out to choose the concentration of vitamin C to be used in experiment II.The negative control is normal saline,added in cup in each plate,vitamin C(100 mg/mL,200 mg/mL,400 mg/mL)was added,the volume in each cup was 100μL.Experiment II:Eight groups of treatments were applied.The first is the negative control(1%normal saline),the second group is the positive control of vitamin C(200 mg/mL).The third,fourth and fifth groups are ciprofloxacin with different concentrations(10 mg/mL,20 mg/mL,40 mg/mL);the sixth,seventh and eighth are the combination of vitamin C with each concentration of ciprofloxacin(10 mg/mL,20 mg/mL,40 mg/mL).Each group includes six petri dishes.Bacterial plates were incubated at 37 oC for 24 h and 48 h.Zone of inhibition is measured in mm.Results and conclusion:Ciprofloxacin produces dose dependent increase in zone of inhibition of S.aureus and E.coli growth,after 24 and 48 hours incubation.While vitamin C in the concentration used produced inhibitory effect on the growth of S.aureus and E.coli,after 24 hours incubation,vitamin C effect was not changed after 48 hours incubation.After 24 hours incubation,vitamin C potentiated the effect of ciprofloxacin at low concentration(10 mg/mL);while vitamin C antagonized the effect of ciprofloxacin at higher concentrations(20 and 40 mg/mL)on S.aureus growth.In the same time,ciprofloxacin antagonized the inhibitory effect of vitamin C on S.aureus growth.After 48 hours incubation,S.aureus produced resistance against ciprofloxacin alone,and that combined with vitamin C compared to zone of inhibition after 24 hours.Ciprofloxacin produced dose dependent inhibition of E.coli growth after incubation for 24 and 48 hours.Vitamin C potentiated the inhibitory effect induced by ciprofloxacin(additive effect).The inhibitory effect of ciprofloxacin,vitamin C and the combination was not changed after 48 hours compared to 24 hours. 展开更多
关键词 CIPROFLOXACIN VITAMIN C s.aureus E.COLI CUP CUT diffusion method
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谈翻译中文体风格的对应性——MissU.S.A和《漂亮的三丫头》原、译文对照评析
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作者 张菁华 《湖北师范学院学报(哲学社会科学版)》 2001年第4期87-91,共5页
本文从语音、词汇、语法和句法结构四个层面 ,对 Miss U .S.A与《漂亮的三丫头》的原、译文作了对照评价 ,说明文体风格的对应在翻译中具有重要的意义和作用。
关键词 翻译 文体风格 对应性 MISS U.s.a 《漂亮的三丫头》
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动漫《S.A特优生》海报的多模态分析
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作者 陈超 《学周刊(下旬)》 2015年第3期23-23,共1页
多模态话语分析已成为语言学研究的热点之一。本文以《S.A特优生》海报为例,揭示其互动意义的构建和体现。
关键词 s.a特优生》海报 多模态 互动意义
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Enlightenment of Cultural Innovation in the Park of China from the Park Development of U.S.A. and Singapore
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作者 Wansong Sun Mingyu Zhang +1 位作者 Wenbing Wu Lili Chen 《Chinese Business Review》 2004年第11期43-46,共4页
This text combines authors' visits and examinations on the spot of Silicon Valley of U.S.A. and Park of Singapore, after analyzing the Park development experience of U.S.A. and Singapore, elucidates "three culture e... This text combines authors' visits and examinations on the spot of Silicon Valley of U.S.A. and Park of Singapore, after analyzing the Park development experience of U.S.A. and Singapore, elucidates "three culture effects" of Park development in terms of cultural system emphatically, then proposes some suggestions of cultural innovation in the Park of China. 展开更多
关键词 U.s.a. SINGAPORE PARK China ENLIGHTENMENT
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Chinese Classical Music Charms U.S.Audiences
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《China Today》 2019年第3期9-9,共1页
A concert by the China National Traditional Orchestra(CNTO)held in the northeast U.S.state of New Jersey on January 29,enthralled thousands of spectators with its music and performance.
关键词 CHINESE CLASSICAL MUSIC U.s.audiences spectators
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The Third Way in the U.S.and Western Europe
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作者 区冰梅 《Contemporary International Relations》 1998年第12期1-8,共8页
In recent years,the political scene in major Western powers has undergone afundamental change in response to the fluid international situation:in the U-nited States,Bill Clinton was elected President in 1992 breaking ... In recent years,the political scene in major Western powers has undergone afundamental change in response to the fluid international situation:in the U-nited States,Bill Clinton was elected President in 1992 breaking Republican controlover White House;while on West European political stage,right-wing ruling pari-ties gave place to the left one after another.Currently,13 out of 15 EU memberstates,except Spain and Ireland,have a government run by left parties or 展开更多
关键词 WEST The Third Way in the U.s.and Western Europe
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New Orientation for ROK-U.S.Alliance
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作者 Guo Xiangang is Senior Research Fellow at China Institute of International Studies. 《China International Studies》 2006年第2期139-150,共12页
关键词 ROK New Orientation for ROK-U.s.alliance
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“Enchanting Paradise” in the U.S.A
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作者 李美娜 《杂技与魔术》 2006年第4期23-,共1页
A 33-member performing group from Zhejiang Acrobatic Troupe went to the U.S.A on May 6, 2006. During their 11-day trip, they at- tended“06 Zhejiang Week”in New York,“Zhejiang Cultral Week”in Los Angeles and“Asia ... A 33-member performing group from Zhejiang Acrobatic Troupe went to the U.S.A on May 6, 2006. During their 11-day trip, they at- tended“06 Zhejiang Week”in New York,“Zhejiang Cultral Week”in Los Angeles and“Asia Arts Festival” 展开更多
关键词 SHOW in the U.s.a Enchanting Paradise
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维美德为智利CMPC Celulosa S.A.公司提供全新的白液生产系统
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作者 李芳 《造纸信息》 2016年第5期83-83,共1页
维美德将向智利CMPC Celulosa S.A.公司的Laja浆厂提供全新的白液生产系统。该系统符合将于2018年生效的环保法令要求,新系统还可提高白液段的生产能力和石灰循环段的控制灵活性。设备预计于2018年2月投入运行。维美德供货是一套包括苛... 维美德将向智利CMPC Celulosa S.A.公司的Laja浆厂提供全新的白液生产系统。该系统符合将于2018年生效的环保法令要求,新系统还可提高白液段的生产能力和石灰循环段的控制灵活性。设备预计于2018年2月投入运行。维美德供货是一套包括苛化设备和石灰窑在内的白液生产系统。该系统可提高碱回收效率。 展开更多
关键词 维美德 智利CMPC Celulosa s.a.公司 白液生产系统 环保法令要求
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和平海运与HACHIMAN SHIPPING S.A.等船舶碰撞损害赔偿纠纷案
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作者 汪洋 《航海》 2002年第3期30-32,共3页
一、当事人名称 原告汕头市和平海运有限公司. 被告HACHIMAN SHIPPING S.A.. 被告DORVAL KAIUN K.K.. 二、当事人诉辨 原告汕头市和平海运有限公司诉称,2000年7月3日,原告所属"和平"轮在长江口水域正常出口航行时,相向行驶的... 一、当事人名称 原告汕头市和平海运有限公司. 被告HACHIMAN SHIPPING S.A.. 被告DORVAL KAIUN K.K.. 二、当事人诉辨 原告汕头市和平海运有限公司诉称,2000年7月3日,原告所属"和平"轮在长江口水域正常出口航行时,相向行驶的被告所属"金亚马"轮突然违章穿越航道,在"和平"轮的正常水道上碰撞了"和平"轮的首部,造成船艏左舷严重受损,原告营运被迫中止,损失达90万元人民币.原告认为,本起事故是由于"金亚马"轮违章航行穿越航道避让措施严重不当所致,被告对此应负90%的责任.据此,原告向本院起诉,请求判令两被告赔偿原告经济损失人民币81万元及利息,并承担诉讼费用及律师费用. 展开更多
关键词 HACHIMAN SHIPPING s.a. 船舶碰撞损害赔偿纠纷案 汕头市和平海运有限公司 海上安全 海事特别诉讼法 海商法
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