The genetic differences among Luffa acutangula （L.） Roxb., Luffa cylindnca （L.） Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test mater...The genetic differences among Luffa acutangula （L.） Roxb., Luffa cylindnca （L.） Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test materials showed a high diversity; the genetic similarity coefficient between both parents was only 0.395; the bands of F1 hybrid mainly consisted of both parents＇; neither FI nor F2 was inherit- ed singly from male or female parent; there was greater genetic variation among F2 population.展开更多
By pot experiment under artificially simulated water stress conditions, soluble protein content, MDA content and SOD, POD, CAT and APX activities in Malus sieversfi leaves were determined to reveal the response mechan...By pot experiment under artificially simulated water stress conditions, soluble protein content, MDA content and SOD, POD, CAT and APX activities in Malus sieversfi leaves were determined to reveal the response mechanism of M. sieversii to changes of relative soil water content. According to the results, with the decrease of relative soil water content, MDA content in M. sieversii leaves increased by mem- brane lipid peroxidation. Cells resist water stress-induced membrane lipid peroxidation and clear the increased reactive oxygen species by improving soluble protein content and SOD, POD, CAT and APX activities. However, various enzymes were involved in the response to water stress under different moisture conditions. In addition, the results indicated that M. sieversii had a good adaptability to higher relative soil water contents.展开更多
A new pentacyclic triterpenoid saponin, named lucyoside Q, was isolated from the leaves of Luffa cylindrica. Its structure was established as 21 β hydroxyloleanolic acid 28 O β D glucopyranoside by means of th...A new pentacyclic triterpenoid saponin, named lucyoside Q, was isolated from the leaves of Luffa cylindrica. Its structure was established as 21 β hydroxyloleanolic acid 28 O β D glucopyranoside by means of the spectral evidence and chemical methods.展开更多
In this study, volatile compounds present in Toona sinensis (A. Juss.) Roem (TS) were investigated and their characteristic aromatic components were identified using Headspace Solid-phase Microextraction (HS-SPME) fol...In this study, volatile compounds present in Toona sinensis (A. Juss.) Roem (TS) were investigated and their characteristic aromatic components were identified using Headspace Solid-phase Microextraction (HS-SPME) followed by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Olfactometry (GC-O). The optimum conditions for extracting the volatiles from TS were achieved with the experimental parameters including the use of a 65 μm polydimethylsiloxane/divinyl benzene (PDMS/DVB) fibre, an extraction temperature of 40℃ and an extraction time of 30 min. Under these conditions, 56 volatile compounds were separated and 53 were identified by GC-MS. Among them, 21 sulfide compounds (42.146%) and 27 terpenes(55.984%) were found to be the major components. The sample was analyzed by GC-O and 26 elutes were sniffed and their sensory descriptions evaluated by an odor panelists. Analysis of the data indicated, two compounds cis and trans isomers of 2-Mercapto-3,4-dimethyl-2,3-dihydrothiophene were major contributors to the characteristic aroma of TS.展开更多
Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus （Narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at...Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus （Narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at lower temperatures during the Chinese Spring Festival, is a traditional Chinese flower with high economic and ornamental value. To study its flower development, a full length cDNA containing MADS box domain from narcissus was isolated by a reverse transcription polymerase chain reaction （RT-PCR） with degenerate oligo-nucleotide primers derived from a conserved MADS- and K-box domain sequence. The 5＇ and the 3＇ regions of the gene were amplified using the PCR protocol for the rapid amplification of cDNA ends （RACE）. The 690 bp open reading frame encodes 230 amino acid residues. A comparison of the deduced amino acid sequence of NTAG with the sequence of other MADS box proteins showed 91.3% amino acid identities with HAG （Hyacinthus orientalis）. Sequence analysis and alignment showed significant similarity with other AG homologues. RNA blot analysis indicated that the narcissus NTAG gene was expressed only in reproductive organs, especially in stamens and carpels. These results indicated that the NTAG gene was an AG homologue and that the AG genes appeared to be structurally and functionally conserved between dicots and monocots.展开更多
According to the relationship between Toona sinensis Roem stand volume,productivity and forest age,site conditions,stand density and other factors,through selecting 8 representative cities or counties,using standard i...According to the relationship between Toona sinensis Roem stand volume,productivity and forest age,site conditions,stand density and other factors,through selecting 8 representative cities or counties,using standard investigation and stem analysis method,this paper makes relatively systematic research about the Toona sinensis Roem plantation accumulation,productivity,and the relationship between them and site conditions.Through comparative analysis indicators of multiple site conditions,this paper expects to give a more comprehensive picture about the source of accumulation and productivity difference.展开更多
[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.m...[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5μm,4.6 mm×250 mm),detection wavelength of 323 nm,column temperature of 25℃,mobile phase of methanol-0.1%phosphoric acid solution,gradient elution and injection volume of 20μL.The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A).[Results]Among the HPLC fingerprints of the 10 batches of C.buchananii Roem.et Schult.materials,there are 13 common peaks,and their similarity is all greater than 0.9,indicating good similarity.[Conclusions]This study can provide a scientific basis for the quality evaluation of C.buchananii Roem.et Schult.展开更多
[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transfor...[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transform infrared spectroscopy. The experimental data were clustered by Omic software and Ward method. [Results] The infrared fingerprints of C. buchananii were obtained, and the similarity was all greater than 0.9. [Conclusions] The established method has good stability and controllability and can be used for the identification of C. buchananii.展开更多
[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of c...[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of chemical liver injury was established by D-GalN, and the mice with D-GalN-induced liver injury were randomly divided into the blank group, model group, positive control group and drug-administered groups. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum of the mice in each group and the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the liver tissue of the mice were determined, and the liver pathological changes were observed. [Results] The extracts of the Zhuang medicinal herb C. buchananii could reduce the serum ALT and AST levels(P<0.05), increase the SOD content in the liver tissue of mice(P<0.05), and decrease the MDA level(P<0.05). [Conclusions] The Zhuang medicinal herb C. buchananii has a good protective effect on D-GalN-induced liver injury in mice.展开更多
基金Supported by Jiangsu Province Science and Technology Support Program,China(BE2012323)Jiangsu Agricultural Science and Technology Innovation Fund[CX(11)1002]~~
文摘The genetic differences among Luffa acutangula （L.） Roxb., Luffa cylindnca （L.） Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test materials showed a high diversity; the genetic similarity coefficient between both parents was only 0.395; the bands of F1 hybrid mainly consisted of both parents＇; neither FI nor F2 was inherit- ed singly from male or female parent; there was greater genetic variation among F2 population.
基金Supported by Science and Technology Innovation Project of Ji'nan City "Identification of Stress-resistant Malus sieversii Germplasm Resources and Screening of Stressresistance Functional Genes"(201401125)~~
文摘By pot experiment under artificially simulated water stress conditions, soluble protein content, MDA content and SOD, POD, CAT and APX activities in Malus sieversfi leaves were determined to reveal the response mechanism of M. sieversii to changes of relative soil water content. According to the results, with the decrease of relative soil water content, MDA content in M. sieversii leaves increased by mem- brane lipid peroxidation. Cells resist water stress-induced membrane lipid peroxidation and clear the increased reactive oxygen species by improving soluble protein content and SOD, POD, CAT and APX activities. However, various enzymes were involved in the response to water stress under different moisture conditions. In addition, the results indicated that M. sieversii had a good adaptability to higher relative soil water contents.
文摘A new pentacyclic triterpenoid saponin, named lucyoside Q, was isolated from the leaves of Luffa cylindrica. Its structure was established as 21 β hydroxyloleanolic acid 28 O β D glucopyranoside by means of the spectral evidence and chemical methods.
文摘In this study, volatile compounds present in Toona sinensis (A. Juss.) Roem (TS) were investigated and their characteristic aromatic components were identified using Headspace Solid-phase Microextraction (HS-SPME) followed by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Olfactometry (GC-O). The optimum conditions for extracting the volatiles from TS were achieved with the experimental parameters including the use of a 65 μm polydimethylsiloxane/divinyl benzene (PDMS/DVB) fibre, an extraction temperature of 40℃ and an extraction time of 30 min. Under these conditions, 56 volatile compounds were separated and 53 were identified by GC-MS. Among them, 21 sulfide compounds (42.146%) and 27 terpenes(55.984%) were found to be the major components. The sample was analyzed by GC-O and 26 elutes were sniffed and their sensory descriptions evaluated by an odor panelists. Analysis of the data indicated, two compounds cis and trans isomers of 2-Mercapto-3,4-dimethyl-2,3-dihydrothiophene were major contributors to the characteristic aroma of TS.
文摘Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus （Narcissus tazetta var. chinensis Roem）, its flowers developed at high temperatures and bloomed at lower temperatures during the Chinese Spring Festival, is a traditional Chinese flower with high economic and ornamental value. To study its flower development, a full length cDNA containing MADS box domain from narcissus was isolated by a reverse transcription polymerase chain reaction （RT-PCR） with degenerate oligo-nucleotide primers derived from a conserved MADS- and K-box domain sequence. The 5＇ and the 3＇ regions of the gene were amplified using the PCR protocol for the rapid amplification of cDNA ends （RACE）. The 690 bp open reading frame encodes 230 amino acid residues. A comparison of the deduced amino acid sequence of NTAG with the sequence of other MADS box proteins showed 91.3% amino acid identities with HAG （Hyacinthus orientalis）. Sequence analysis and alignment showed significant similarity with other AG homologues. RNA blot analysis indicated that the narcissus NTAG gene was expressed only in reproductive organs, especially in stamens and carpels. These results indicated that the NTAG gene was an AG homologue and that the AG genes appeared to be structurally and functionally conserved between dicots and monocots.
文摘According to the relationship between Toona sinensis Roem stand volume,productivity and forest age,site conditions,stand density and other factors,through selecting 8 representative cities or counties,using standard investigation and stem analysis method,this paper makes relatively systematic research about the Toona sinensis Roem plantation accumulation,productivity,and the relationship between them and site conditions.Through comparative analysis indicators of multiple site conditions,this paper expects to give a more comprehensive picture about the source of accumulation and productivity difference.
基金Supported by Fundamental Scientific Research Ability Enhancement Project for Young and Middle-Aged Teachers in Universities and Colleges of Guangxi(2019KY0324)Graduate Project of Guangxi University of Chinese Medicine(xjyb019)+2 种基金Project of Zhuang Medicine Collaborative Innovation Center(zyx[2015]-01)Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)College Students’Innovative Entrepreneurial Training Program of Guangxi University of Chinese Medicine(201910600022).
文摘[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5μm,4.6 mm×250 mm),detection wavelength of 323 nm,column temperature of 25℃,mobile phase of methanol-0.1%phosphoric acid solution,gradient elution and injection volume of 20μL.The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A).[Results]Among the HPLC fingerprints of the 10 batches of C.buchananii Roem.et Schult.materials,there are 13 common peaks,and their similarity is all greater than 0.9,indicating good similarity.[Conclusions]This study can provide a scientific basis for the quality evaluation of C.buchananii Roem.et Schult.
基金Supported by The Basic Ability Improving Project of Young and Middle-aged Teachers in Guangxi(2019KY0324)Graduate Program of Guangxi University of Chinese Medicine(xjyb019)+2 种基金Collaborative Innovation Center for Zhuang and Yao Medicines(zyx[2015]-01)General Project of Guangxi University of Chinese Medicine(P2010016)Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)
文摘[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transform infrared spectroscopy. The experimental data were clustered by Omic software and Ward method. [Results] The infrared fingerprints of C. buchananii were obtained, and the similarity was all greater than 0.9. [Conclusions] The established method has good stability and controllability and can be used for the identification of C. buchananii.
基金Supported by The Basic Ability Improving Project of Young and Middle-aged Teachers in Guangxi(2019KY0324)Graduate Program of Guangxi University of Chinese Medicine(YCSY20190096)+2 种基金Collaborative Innovation Center for Zhuang and Yao Medicines(zyx[2015]-01)Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)College Students’Innovation and Enterpreneurship Training Program of Guangxi University of Chinese Medicine(201910600022)
文摘[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of chemical liver injury was established by D-GalN, and the mice with D-GalN-induced liver injury were randomly divided into the blank group, model group, positive control group and drug-administered groups. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum of the mice in each group and the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the liver tissue of the mice were determined, and the liver pathological changes were observed. [Results] The extracts of the Zhuang medicinal herb C. buchananii could reduce the serum ALT and AST levels(P<0.05), increase the SOD content in the liver tissue of mice(P<0.05), and decrease the MDA level(P<0.05). [Conclusions] The Zhuang medicinal herb C. buchananii has a good protective effect on D-GalN-induced liver injury in mice.
