AIM: To investigate the loci of adefovir dipivoxil (ADV)-induced resistance in hepatitis B virus (HBV) isolates and optimize the management of ADV-treated patients.
Objective.To evaluate the effects of high-fructose diet on expression of orex in and its receptors,orexin1receptor and orexin2receptor in rat hypothalamus tissue,and to analysis the interaction of related fa ctors inv...Objective.To evaluate the effects of high-fructose diet on expression of orex in and its receptors,orexin1receptor and orexin2receptor in rat hypothalamus tissue,and to analysis the interaction of related fa ctors involved in regulating orexin and its receptors.Methods.Insulin resistan ce rat model induced by high fructose confirmed by the gold standard eug-lycaem ic clamping was employed and mRNA expression of orexin and its receptors OX 1 R and OX 2 R in hypothalamus,mRNA expression of leptin in adipose tissue were measured by reverse transcription poly-merase chain reaction.Serum insulin and triglyc eride levels were measured by chemiluminescence im-munoassay and biochemical en zyme techniques.Results.Expression of orexin mRNA decreased about 40%in hi gh fructose diet rats compared to control group,whereas expressi on of orexin1receptor and orexin2receptor mRNA increased up to4.4and5.1f old.Leptin mRNA expression in adipose tissue increased about 3 0%in comparison with control group.Blood glucose,serum insuli n and triglyceride have shown signi ?ficant higher levels than those in contro l group.Glucose infusion rate was much low-er in comparison with control group.Conclusi ons.High?fructose diet induces insulin resistance in rats with impact on orexi n and leptin regulations.Blood glucose,serum insulin,lipid metabolism and lep tin play an interactive role on orexin and its receptors regulation in rats.展开更多
目的分析非酮症糖尿病患者糖化血红蛋白(HbA_(1c))与红细胞分布宽度变异系数(RDW-CV)和甲状腺过氧化物酶抗体(TPOAb)阳性率的相关性,并探讨HbA_(1c)变化的影响因素。方法选取2014年3月—2016年4月在益阳市中心医院内分泌科住院的符合纳...目的分析非酮症糖尿病患者糖化血红蛋白(HbA_(1c))与红细胞分布宽度变异系数(RDW-CV)和甲状腺过氧化物酶抗体(TPOAb)阳性率的相关性,并探讨HbA_(1c)变化的影响因素。方法选取2014年3月—2016年4月在益阳市中心医院内分泌科住院的符合纳入标准的非酮症糖尿病患者413例。依据患者HbA_(1c)进行分组:HbA_(1c)≤7.5%组(A组,n=128)、7.5%<HbA_(1c)<10.0%组(B组,n=190)、HbA_(1c)≥10.0%组(C组,n=95)。比较3组患者性别、年龄、病程、体质指数(BMI)、RDW-CV、低密度脂蛋白(LDL)、总胆固醇(TC)、24 h尿微量清蛋白(24 h UAE)、C反应蛋白(CRP)、空腹C肽(FCP)、餐后2 h C肽(PCP)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)、谷氨酸脱羟酶抗体(GADA)阳性率、TPOAb阳性率,分析HbA_(1c)与上述指标的相关性及影响患者HbA_(1c)变化的独立危险因素。结果 B组、C组患者病程长于A组,RDW-CV大于A组,TSH及TPOAb阳性率高于A组(P<0.05);C组患者病程长于B组,RDW-CV大于B组,TSH、TPOAb阳性率高于B组(P<0.05)。Spearman秩相关分析结果显示,患者HbA_(1c)与病程、RDW-CV、TPOAb阳性率、TSH呈正相关(rs值分别为0.681、0.630、0.432、0.438,P值分别为<0.001、<0.001、0.001、0.001)。RDW-CV[OR=2.024,95%CI(1.237,4.103)]、TPOAb阳性率[OR=1.243,95%CI(1.134,1.548)]、TSH[OR=1.814,95%CI(1.567,3.234)]是非酮症糖尿病患者HbA_(1c)变化的独立危险因素(P<0.05)。结论非酮症糖尿病患者HbA_(1c)与RDW-CV、TPOAb阳性率呈正相关,且RDW-CV、TPOAb阳性率是患者HbA_(1c)变化的独立危险因素,因此,血糖控制越差,红细胞形态改变越明显,免疫性甲状腺疾病发生的可能性越大。展开更多
基金Supported by BaoenWang Liver Fibrosis Research Fund of the Chinese Foundation for Hepatitis Prevention and Control,No.20100009Zhejiang Medicine and Health Sciences Research Fund,No.2008B167 and No.2014PYA018+3 种基金Nanjing Military Area Command of the PLA Medical Technology Innovation,No.11MA019Ningbo Health Technology Project for Excellent Young and Middle-aged Talent,No.2011-145Ningbo Leader and Top Notch Person Training Project,No.2012-131Zhejiang Medicine and Health Project for Excellent Young Talent,No.2013-245
文摘AIM: To investigate the loci of adefovir dipivoxil (ADV)-induced resistance in hepatitis B virus (HBV) isolates and optimize the management of ADV-treated patients.
文摘Objective.To evaluate the effects of high-fructose diet on expression of orex in and its receptors,orexin1receptor and orexin2receptor in rat hypothalamus tissue,and to analysis the interaction of related fa ctors involved in regulating orexin and its receptors.Methods.Insulin resistan ce rat model induced by high fructose confirmed by the gold standard eug-lycaem ic clamping was employed and mRNA expression of orexin and its receptors OX 1 R and OX 2 R in hypothalamus,mRNA expression of leptin in adipose tissue were measured by reverse transcription poly-merase chain reaction.Serum insulin and triglyc eride levels were measured by chemiluminescence im-munoassay and biochemical en zyme techniques.Results.Expression of orexin mRNA decreased about 40%in hi gh fructose diet rats compared to control group,whereas expressi on of orexin1receptor and orexin2receptor mRNA increased up to4.4and5.1f old.Leptin mRNA expression in adipose tissue increased about 3 0%in comparison with control group.Blood glucose,serum insuli n and triglyceride have shown signi ?ficant higher levels than those in contro l group.Glucose infusion rate was much low-er in comparison with control group.Conclusi ons.High?fructose diet induces insulin resistance in rats with impact on orexi n and leptin regulations.Blood glucose,serum insulin,lipid metabolism and lep tin play an interactive role on orexin and its receptors regulation in rats.
文摘目的分析非酮症糖尿病患者糖化血红蛋白(HbA_(1c))与红细胞分布宽度变异系数(RDW-CV)和甲状腺过氧化物酶抗体(TPOAb)阳性率的相关性,并探讨HbA_(1c)变化的影响因素。方法选取2014年3月—2016年4月在益阳市中心医院内分泌科住院的符合纳入标准的非酮症糖尿病患者413例。依据患者HbA_(1c)进行分组:HbA_(1c)≤7.5%组(A组,n=128)、7.5%<HbA_(1c)<10.0%组(B组,n=190)、HbA_(1c)≥10.0%组(C组,n=95)。比较3组患者性别、年龄、病程、体质指数(BMI)、RDW-CV、低密度脂蛋白(LDL)、总胆固醇(TC)、24 h尿微量清蛋白(24 h UAE)、C反应蛋白(CRP)、空腹C肽(FCP)、餐后2 h C肽(PCP)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)、谷氨酸脱羟酶抗体(GADA)阳性率、TPOAb阳性率,分析HbA_(1c)与上述指标的相关性及影响患者HbA_(1c)变化的独立危险因素。结果 B组、C组患者病程长于A组,RDW-CV大于A组,TSH及TPOAb阳性率高于A组(P<0.05);C组患者病程长于B组,RDW-CV大于B组,TSH、TPOAb阳性率高于B组(P<0.05)。Spearman秩相关分析结果显示,患者HbA_(1c)与病程、RDW-CV、TPOAb阳性率、TSH呈正相关(rs值分别为0.681、0.630、0.432、0.438,P值分别为<0.001、<0.001、0.001、0.001)。RDW-CV[OR=2.024,95%CI(1.237,4.103)]、TPOAb阳性率[OR=1.243,95%CI(1.134,1.548)]、TSH[OR=1.814,95%CI(1.567,3.234)]是非酮症糖尿病患者HbA_(1c)变化的独立危险因素(P<0.05)。结论非酮症糖尿病患者HbA_(1c)与RDW-CV、TPOAb阳性率呈正相关,且RDW-CV、TPOAb阳性率是患者HbA_(1c)变化的独立危险因素,因此,血糖控制越差,红细胞形态改变越明显,免疫性甲状腺疾病发生的可能性越大。
