Histone deacetylase （HDAC1） has a high expression in many cancer cells and curcumin can inhibit the growth of cancer cells. This paper was designed to investigate the expression of HDAC1 of Raji cells and the effect...Histone deacetylase （HDAC1） has a high expression in many cancer cells and curcumin can inhibit the growth of cancer cells. This paper was designed to investigate the expression of HDAC1 of Raji cells and the effect of curcumin on their proliferation and apoptosis. Raji cells were treated with 3. 125-50 μmol/L curcumin for 8--48 h and the growth inhibition rates of Raji cells were measured by MTT. The expression of HDAC1 on Raji cells were examined by mRNA, Western blot at 24 h various concertrations （1.6- 50 μmol/L）. Curcumin could selectively inhibit the proliferation of Raji cells in a dose- and time-dependent manner, with the inhibition rate being 52.47 %--82.18 % （P〈0. 01）. The up-regulation of HDAC1 expression was observed within 24 h after the treatment with curcumin as shown by RT-PCR and Western blot. With the increase of concentration, the expression was down-regulated in a dose- dependent manner. It is concluded that the expression of HDAC1 plays an important role in the proliferation and apoptosis of Raji cells and curcumin can inhibit the growth of Raji cells at various concentrations and promote the apoptosis of Raji cells.展开更多
Dr.Raj Reddy is the Moza Bint Nasser University Professor of Computer Science and Robotics in the School of Computer Science,Carnegie Mellon University,Pittsburgh,Pennsylvania,USA.He was awarded the ACM Turing Award i...Dr.Raj Reddy is the Moza Bint Nasser University Professor of Computer Science and Robotics in the School of Computer Science,Carnegie Mellon University,Pittsburgh,Pennsylvania,USA.He was awarded the ACM Turing Award in 1994.展开更多
The importance of open and unbuilt neighborhood spaces in residential developments has been established by numerous scholars.Having a diverse nomenclature in varied contexts,these areas are indispensable yet often neg...The importance of open and unbuilt neighborhood spaces in residential developments has been established by numerous scholars.Having a diverse nomenclature in varied contexts,these areas are indispensable yet often neglected and designated as leftovers in contemporary planning practice.With their usefulness in the Indian context of socially active and sometimes even intrusive communities,such spaces are rapidly losing their place in present-day residential environments due to the pressure of providing for the rapidly growing population coupled with mass migratory patterns.These neighborhood spaces that impart physical,psychological,and perceptual comfort to residents have been present in spontaneously developed traditional Indian cities due to their inherent order and integrity.Moreover,some contemporary housing environments by eminent designers have also utilized the virtues of well-designed neighborhood environments.This study is a comparative analysis of three selected projects,which aspire to devise suitable methods for contemporary Indian context and achieve neighborhood comfort and an overall sense of belongingness among residents.展开更多
基金This project was supported by a grant from the NationalNatural Sciences Foundation of China (No .30271672)
文摘Histone deacetylase （HDAC1） has a high expression in many cancer cells and curcumin can inhibit the growth of cancer cells. This paper was designed to investigate the expression of HDAC1 of Raji cells and the effect of curcumin on their proliferation and apoptosis. Raji cells were treated with 3. 125-50 μmol/L curcumin for 8--48 h and the growth inhibition rates of Raji cells were measured by MTT. The expression of HDAC1 on Raji cells were examined by mRNA, Western blot at 24 h various concertrations （1.6- 50 μmol/L）. Curcumin could selectively inhibit the proliferation of Raji cells in a dose- and time-dependent manner, with the inhibition rate being 52.47 %--82.18 % （P〈0. 01）. The up-regulation of HDAC1 expression was observed within 24 h after the treatment with curcumin as shown by RT-PCR and Western blot. With the increase of concentration, the expression was down-regulated in a dose- dependent manner. It is concluded that the expression of HDAC1 plays an important role in the proliferation and apoptosis of Raji cells and curcumin can inhibit the growth of Raji cells at various concentrations and promote the apoptosis of Raji cells.
文摘Dr.Raj Reddy is the Moza Bint Nasser University Professor of Computer Science and Robotics in the School of Computer Science,Carnegie Mellon University,Pittsburgh,Pennsylvania,USA.He was awarded the ACM Turing Award in 1994.
文摘The importance of open and unbuilt neighborhood spaces in residential developments has been established by numerous scholars.Having a diverse nomenclature in varied contexts,these areas are indispensable yet often neglected and designated as leftovers in contemporary planning practice.With their usefulness in the Indian context of socially active and sometimes even intrusive communities,such spaces are rapidly losing their place in present-day residential environments due to the pressure of providing for the rapidly growing population coupled with mass migratory patterns.These neighborhood spaces that impart physical,psychological,and perceptual comfort to residents have been present in spontaneously developed traditional Indian cities due to their inherent order and integrity.Moreover,some contemporary housing environments by eminent designers have also utilized the virtues of well-designed neighborhood environments.This study is a comparative analysis of three selected projects,which aspire to devise suitable methods for contemporary Indian context and achieve neighborhood comfort and an overall sense of belongingness among residents.
