To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuc...To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.展开更多
funded by the program supported by the National Key Technologies R＆D Program of China during the 11th Five-Year Plan period （2009BADA8B02）;the Fundamental Research Funds for the Central Universities, China （KYZ201...funded by the program supported by the National Key Technologies R＆D Program of China during the 11th Five-Year Plan period （2009BADA8B02）;the Fundamental Research Funds for the Central Universities, China （KYZ201202-3）展开更多
The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utiliz...The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utilized a cognitive disorder mouse model induced by scopolamine and observed that daily oral administration of SFGDI at a dosage of 40 mg/kg resulted in improved behavioral performance in the Passive avoidance test,the Channel-type water maze test,and the Novel object recognition test(P<0.05).Furthermore,our investigation into the underlying neuroprotective mechanisms of SFGDI revealed that it reduced oxidative stress levels through the activation of the Sirt3/SOD/ROS pathway,thereby balancing hippocampal unsaturated lipids(P<0.05)in the mouse hippocampus.Moreover,SFGDI significantly decreased hippocampal neuroinflammation by modu-lating the activation of microglia and hypertrophy of astrocytes.Additionally,SFGDI was found to enhance synaptic plasticity in the mouse hippocampus by activating the ChAT/p-CaMKII/BDNF pathway and increasing spine density in neuron cells(P<0.05),particularly mushroom spines(P<0.05).These experiments demon-strate that SFGDI exhibits neuroprotective effects by regulating hippocampal oxidative stress through the Sirt3/SOD/ROS pathway and synaptic plasticity via the ChAT/p-CaMKII/BDNF pathway in the mouse hippocampus,thus emerging as a potential neuro-nutraceutical agent for alleviating memory impairment.展开更多
A series of three-dimensional Ln-based coordination frameworks were designed and synthesized.After nanolization,they show great potential as efficient and low toxic X-ray responsive radiosensitizers for the treatment ...A series of three-dimensional Ln-based coordination frameworks were designed and synthesized.After nanolization,they show great potential as efficient and low toxic X-ray responsive radiosensitizers for the treatment of cervical cancer.The experimental data showed that these Ln-based nanoframeworks induced G0/G1 arrest mainly through the increase of the intracellular ROS level.展开更多
基金supported by the Fund of China Agriculture Research System(CARS-37)the Genetically Modified Organisms Breeding Major Projects of China(2009ZX08011-031B)+1 种基金the Basic Research Fund of IAS,CAAS(2010jc-3-1)the National Natural Science Foundation of China(31001011)
文摘To our knowledge,no single study has systemically compared cryopreservation efficiencies of bovine blastocysts derived from in vitro fertilization （IVF）,intracytoplasmic sperm injection （ICSI） and somatic cell nuclear transfer （SCNT） by controlled freezing and vitrification.This experiment,therefore,was designed to compare the cryopreservation of these blastocysts with controlled freezing and OPS vitrification.Adenosine-5’-triphosphate （ATP） content and reactive oxygen species （ROS） level in blastocysts were also analyzed.Firstly,for each type of blastocyst （IVF,ICSI or SCNT）,significant differences were observed between the survival rates of the controlled freezing （（81.56±2.33）,（68.18±4.72） or （47.89±5.83）%） and OPS vitrification groups （（92.24±4.54）,（82.40±3.76） or （78.71±5.91）%;P〈0.05）.Secondly,for each type of blastocyst （IVF,ICSI or SCNT）,ATP content was significantly decreased after controlled freezing or vitrification,and the ATP content in the controlled freezing group （0.43±0.06）,（0.35±0.05） or （0.21±0.02） pmol） was significantly lower than that found in the OPS vitrification group （0.62±0.04）,（0.46±0.03） or （0.30±0.01） pmol;P〈0.05）.Thirdly,ROS level in fresh IVF （（47.33±3.56） c.p.s （counted photons per second）,ICSI （（36.51±2.58） c.p.s） or SCNT blastocysts （（26.44±1.49） c.p.s） was significantly lower than that found in the OPS vitrification group （（72.14±4.31）,（58.89±3.89） or （40.11±5.73） c.p.s;P〈0.05）,but higher than that of the controlled freezing group （34.41±3.32）,（23.13±1.26） or （15.46±2.45） c.p.s;P〈0.05）.The present study indicated that vitrification is more efficient in the cryopreservation of bovine blastocysts derived from IVF,ICSI or SCNT than controlled freezing.Furthermore,both vitrification and controlled freezing significantly altered the ATP content and ROS level in those blastocysts.
基金funded by the program supported by the National Key Technologies R&D Program of China during the 11th Five-Year Plan period (2009BADA8B02)the Fundamental Research Funds for the Central Universities, China (KYZ201202-3)
文摘funded by the program supported by the National Key Technologies R＆D Program of China during the 11th Five-Year Plan period （2009BADA8B02）;the Fundamental Research Funds for the Central Universities, China （KYZ201202-3）
基金supported by the National Key Research and Devel-opment Program of China(2017YFD0400500)the Graduate Inno-vation Fund of Dalian Polytechnic University.
文摘The peptide Ser-Phe-Gly-Asp-Ile(SFGDI),derived from sea cucumber,has demonstrated neuroprotective effects in cell models;however,the in vivo neuroprotective efficacy of SFGDI remains unexplored.In this study,we utilized a cognitive disorder mouse model induced by scopolamine and observed that daily oral administration of SFGDI at a dosage of 40 mg/kg resulted in improved behavioral performance in the Passive avoidance test,the Channel-type water maze test,and the Novel object recognition test(P<0.05).Furthermore,our investigation into the underlying neuroprotective mechanisms of SFGDI revealed that it reduced oxidative stress levels through the activation of the Sirt3/SOD/ROS pathway,thereby balancing hippocampal unsaturated lipids(P<0.05)in the mouse hippocampus.Moreover,SFGDI significantly decreased hippocampal neuroinflammation by modu-lating the activation of microglia and hypertrophy of astrocytes.Additionally,SFGDI was found to enhance synaptic plasticity in the mouse hippocampus by activating the ChAT/p-CaMKII/BDNF pathway and increasing spine density in neuron cells(P<0.05),particularly mushroom spines(P<0.05).These experiments demon-strate that SFGDI exhibits neuroprotective effects by regulating hippocampal oxidative stress through the Sirt3/SOD/ROS pathway and synaptic plasticity via the ChAT/p-CaMKII/BDNF pathway in the mouse hippocampus,thus emerging as a potential neuro-nutraceutical agent for alleviating memory impairment.
基金supported by the National Natural Science Foundation of China(21877049)Major Program for Tackling Key Problems of Industrial Technology in Guangzhou(201902020013)+5 种基金Dedicated Fund for Promoting High-Quality Marine Economic Development in Guangdong Province(GDOE-2019-A31 and 2020-035)Guangzhou Key Laboratory of Molecular and Functional Imaging for Clinical Translation(201905010003)Innovation Team Project in Guangdong Colleges and Universities(2019KCXTD008 and 2019KTSCX012)Opening Fund of Hubei Key Laboratory of Bioinorganic Chemistry&Materia Medica(BCMM202001)Guangdong Basic and Applied Basic Research Foundation(2020B1515120043)K.C.Wong Education Foundation.
文摘A series of three-dimensional Ln-based coordination frameworks were designed and synthesized.After nanolization,they show great potential as efficient and low toxic X-ray responsive radiosensitizers for the treatment of cervical cancer.The experimental data showed that these Ln-based nanoframeworks induced G0/G1 arrest mainly through the increase of the intracellular ROS level.
