Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment...Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment.Methods:Public databases were employed to identify Cedrenol,an active component of Bupleuri Radix,and its associated gene targets.Among these,the gene RORC(also known as RORγ)was selected for detailed analysis due to its high expression correlation.Expression levels of RORC across various cancers were assessed using the TCGA pan-cancer dataset.Further analyses,including differential expression,prognostic relevance,immune infiltration,single-gene,and functional enrichment analyses,were conducted using the TCGA ccRCC dataset.Additionally,potential drug sensitivities and molecular docking interactions with RORC were explored using the GSCALite and CellMiner databases.The effects of RORC on ccRCC were also validated through cellular experiments.Results:RORC exhibited elevated expression in clear cell renal carcinoma tissue in contrast to normal tissues,and lower RORC expression was related to better prognosis.Immune cell infiltration analysis suggested that RORC may influence the penetration of cells that inhibit immune responses,such as regulatory T cells,thereby affecting ccRCC progression.Furthermore,molecular docking studies revealed that several drugs,including Axitinib,Docetaxel,Methotrexate,and Temsirolimus,have a high affinity for RORC and exhibit strong molecular binding.In cellular experiment experiments confirmed that RORC knockdown led to reduced proliferation,metastasis,and ccRCC cell invasion.Conclusion:The study implies that RORC may be a potential gene target for Bupleuri Radix in the management of ccRCC.展开更多
【目的】研究淋巴细胞亚群Th17与Treg上游调控基因维甲酸相关孤儿核受体(RORC)、叉状头转录因子P3(Foxp3)mRNA在支气管哮喘患儿外周血单个核细胞中的表达变化,并探讨其临床意义。【方法】采用荧光实时定量PCR技术分别检测20例哮喘发作期...【目的】研究淋巴细胞亚群Th17与Treg上游调控基因维甲酸相关孤儿核受体(RORC)、叉状头转录因子P3(Foxp3)mRNA在支气管哮喘患儿外周血单个核细胞中的表达变化,并探讨其临床意义。【方法】采用荧光实时定量PCR技术分别检测20例哮喘发作期(年龄8.5±2.3)和20例哮喘缓解期(年龄9.2±1.8)外周血单个核细胞的RORC和Foxp3mRNA表达水平,并与10例健康对照组(年龄8.0±1.6)进行比较。并将哮喘患儿RORC和Foxp3 mRNA表达水平与其同期肺功能第一秒用力呼气容积(FEV1)进行相关分析。【结果】哮喘发作组RORC mRNA水平显著高于缓解组和正常对照组(5.41±0.12 vs 3.33±0.27 vs 3.78±0.13,P<0.01);哮喘发作组Foxp3 mRNA水平显著低于缓解组和正常对照组(3.34±0.12 vs 4.43±0.07 vs 4.13±0.04,P<0.01);缓解组和正常对照组相比,RORC和Foxp3 mRNA水平均无明显差异(P>0.05)。哮喘发作组RORC mRNA/Foxp3 mRNA比值明显高于缓解组和正常对照组(1.68±0.09 vs 0.76±0.06 vs 0.92±0.04,P<0.01)。Pearson相关分析显示,哮喘患儿外周血RORC mRNA水平与FEV1成负相关,而Foxp3 mRNA水平与FEV1成正相关(r分别为-0.58和0.52,P<0.01)。【结论】RORC mRNA/Foxp3 mRNA在发作期哮喘患儿存在失衡表达,提示Th17/Treg失衡可能在哮喘的急性发作及肺功能损伤中起着重要作用。展开更多
基金supported by Hubei Provincial Department of Science and Technology,Innovation and Development Joint Fund(No.2022CFD145)Young Talents Project of Hubei Provincial Administration of Traditional Chinese Medicine(No.ZY2023Q002).
文摘Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment.Methods:Public databases were employed to identify Cedrenol,an active component of Bupleuri Radix,and its associated gene targets.Among these,the gene RORC(also known as RORγ)was selected for detailed analysis due to its high expression correlation.Expression levels of RORC across various cancers were assessed using the TCGA pan-cancer dataset.Further analyses,including differential expression,prognostic relevance,immune infiltration,single-gene,and functional enrichment analyses,were conducted using the TCGA ccRCC dataset.Additionally,potential drug sensitivities and molecular docking interactions with RORC were explored using the GSCALite and CellMiner databases.The effects of RORC on ccRCC were also validated through cellular experiments.Results:RORC exhibited elevated expression in clear cell renal carcinoma tissue in contrast to normal tissues,and lower RORC expression was related to better prognosis.Immune cell infiltration analysis suggested that RORC may influence the penetration of cells that inhibit immune responses,such as regulatory T cells,thereby affecting ccRCC progression.Furthermore,molecular docking studies revealed that several drugs,including Axitinib,Docetaxel,Methotrexate,and Temsirolimus,have a high affinity for RORC and exhibit strong molecular binding.In cellular experiment experiments confirmed that RORC knockdown led to reduced proliferation,metastasis,and ccRCC cell invasion.Conclusion:The study implies that RORC may be a potential gene target for Bupleuri Radix in the management of ccRCC.
文摘【目的】研究淋巴细胞亚群Th17与Treg上游调控基因维甲酸相关孤儿核受体(RORC)、叉状头转录因子P3(Foxp3)mRNA在支气管哮喘患儿外周血单个核细胞中的表达变化,并探讨其临床意义。【方法】采用荧光实时定量PCR技术分别检测20例哮喘发作期(年龄8.5±2.3)和20例哮喘缓解期(年龄9.2±1.8)外周血单个核细胞的RORC和Foxp3mRNA表达水平,并与10例健康对照组(年龄8.0±1.6)进行比较。并将哮喘患儿RORC和Foxp3 mRNA表达水平与其同期肺功能第一秒用力呼气容积(FEV1)进行相关分析。【结果】哮喘发作组RORC mRNA水平显著高于缓解组和正常对照组(5.41±0.12 vs 3.33±0.27 vs 3.78±0.13,P<0.01);哮喘发作组Foxp3 mRNA水平显著低于缓解组和正常对照组(3.34±0.12 vs 4.43±0.07 vs 4.13±0.04,P<0.01);缓解组和正常对照组相比,RORC和Foxp3 mRNA水平均无明显差异(P>0.05)。哮喘发作组RORC mRNA/Foxp3 mRNA比值明显高于缓解组和正常对照组(1.68±0.09 vs 0.76±0.06 vs 0.92±0.04,P<0.01)。Pearson相关分析显示,哮喘患儿外周血RORC mRNA水平与FEV1成负相关,而Foxp3 mRNA水平与FEV1成正相关(r分别为-0.58和0.52,P<0.01)。【结论】RORC mRNA/Foxp3 mRNA在发作期哮喘患儿存在失衡表达,提示Th17/Treg失衡可能在哮喘的急性发作及肺功能损伤中起着重要作用。
