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基于RhoA/ROCKⅡ通路探讨水蛭改善脑缺血/再灌注小鼠脑损伤的机制
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作者 赵磊 曹玉爽 +4 位作者 郭莉琛 柴丽娟 王少峡 袁庆 胡利民 《中国药理学通报》 北大核心 2026年第1期100-106,共7页
目的探究水蛭提取物(leech)调控Rho激酶(Rhoassociated coiled-coil-forming kinase,ROCK)通路保护大脑中动脉闭塞/再灌注(middle cerebral artery occlusion/reperfu-sion,MCAO/R)小鼠血脑屏障及其作用机制。方法采用大脑中动脉线栓法... 目的探究水蛭提取物(leech)调控Rho激酶(Rhoassociated coiled-coil-forming kinase,ROCK)通路保护大脑中动脉闭塞/再灌注(middle cerebral artery occlusion/reperfu-sion,MCAO/R)小鼠血脑屏障及其作用机制。方法采用大脑中动脉线栓法建立MCAO/R模型后,将SPF级C57BL/6(C57BL/6JNifdc)雄性小鼠,分为假手术(Sham)组、MCAO/R组、阿司匹林组(aspirin,13 mg·kg^(-1))、水蛭提取液组(leech,0.8、1.6、2.4 mL·kg^(-1))共6组。TTC染色观察脑梗死体积,mNSS评分评价神经缺损情况;HE染色及Nissl染色观察各组小鼠脑缺血半暗带病理改变;免疫荧光检测Laminin,PDGFRβ/Biotinylated LEL免疫荧光双染检测脑缺血半暗带周细胞与脑微血管内皮细胞解离情况;Western blot检测RhoA、ROCKⅡ蛋白,紧密连接相关蛋白ZO-1、Occludin、Clau-din-5,基底膜(BM)相关蛋白层粘连蛋白(LN)、纤维连接蛋白(FN)及金属基质蛋白酶MMP-2、MMP-9蛋白,以及p-MLC/MLC蛋白表达情况。结果Leech明显改善小鼠神经缺损,减轻脑梗死体积(P<0.05,P<0.01);Western blot结果显示,leech可上调ZO-1、Occludin、Claudin-5、Laminin、Fibronectin蛋白表达,降低p-MLC/MLC、RhoA、ROCKⅡ、MMP-2、MMP-9蛋白表达(P<0.05,P<0.01);免疫荧光结果显示,leech可明显提高Laminin及PDGFRβ/Biotinylated LEL免疫荧光双染的表达。结论Leech可以改善MCAO/R小鼠脑缺血半暗带BBB损伤,其机制可能是通过调节RhoA/ROCKⅡ信号通路有关。 展开更多
关键词 水蛭 脑缺血/再灌注损伤 RhoA/rockⅡ信号通路 血脑屏障 紧密连接 基质金属蛋白酶
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Quantitative principles of dynamic interaction between rock support and surrounding rock in rockburst roadways 被引量:3
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作者 Lianpeng Dai Dingjie Feng +4 位作者 Yishan Pan Aiwen Wang Ying Ma Yonghui Xiao Jianzhuo Zhang 《International Journal of Mining Science and Technology》 2025年第1期41-55,共15页
Rockbursts, which mainly affect mining roadways, are dynamic disasters arising from the surrounding rock under high stress. Understanding the interaction between supports and the surrounding rock is necessary for effe... Rockbursts, which mainly affect mining roadways, are dynamic disasters arising from the surrounding rock under high stress. Understanding the interaction between supports and the surrounding rock is necessary for effective rockburst control. In this study, the squeezing behavior of the surrounding rock is analyzed in rockburst roadways, and a mechanical model of rockbursts is established considering the dynamic support stress, thus deriving formulas and providing characteristic curves for describing the interaction between the support and surrounding rock. Design principles and parameters of supports for rockburst control are proposed. The results show that only when the geostress magnitude exceeds a critical value can it drive the formation of rockburst conditions. The main factors influencing the convergence response and rockburst occurrence around roadways are geostress, rock brittleness, uniaxial compressive strength, and roadway excavation size. Roadway support devices can play a role in controlling rockburst by suppressing the squeezing evolution of the surrounding rock towards instability points of rockburst. Further, the higher the strength and the longer the impact stroke of support devices with constant resistance, the more easily multiple balance points can be formed with the surrounding rock to control rockburst occurrence. Supports with long impact stroke allow adaptation to varying geostress levels around the roadway, aiding in rockburst control. The results offer a quantitative method for designing support systems for rockburst-prone roadways. The design criterion of supports is determined by the intersection between the convergence curve of the surrounding rock and the squeezing deformation curve of the support devices. 展开更多
关键词 Deep roadway rockBURST Dynamic interaction rock support Surrounding rock rockburst control
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Advancements in Laboratory Studies of Layered Rock Masses for Deep Engineering:Insights and Future Perspectives
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作者 Yan Zhang Kai Meng +6 位作者 Xuanmei Fan Guoqing Chen Xiangsheng Zheng Shaojun Li Tianbin Li Peng Zeng Min Xia 《Journal of Earth Science》 2025年第3期1334-1340,共7页
Layered rock masses represent complex geological formations commonly encountered in the surrounding rock of deep engineering excavations(Hou et al.,2019;Xu et al.,2017;Yang C H et al.,2009;Xian and Tan,1989).These roc... Layered rock masses represent complex geological formations commonly encountered in the surrounding rock of deep engineering excavations(Hou et al.,2019;Xu et al.,2017;Yang C H et al.,2009;Xian and Tan,1989).These rock masses are predominantly composed of sedimentary,para-metamorphic,and volcanic rock types,characterized by a set of prominent,primary bedding structural planes(layers)exhibiting relatively consistent orientations and significant spatial continuity. 展开更多
关键词 surrounding rock rock masses deep engineering laboratory studies geological formations layered rock masses sedimentary rocks deep engineering excavations hou
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Impact rock-breaking mechanisms and energy transfer laws of conical tooth bits in hot dry rocks
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作者 LIU Qingyou HUANG Tao 《Petroleum Exploration and Development》 2025年第4期1053-1063,共11页
Based on the finite-discrete element method,a three-dimensional numerical model for axial impact rock breaking was established and validated.A computational method for energy conversion during impact rock breaking was... Based on the finite-discrete element method,a three-dimensional numerical model for axial impact rock breaking was established and validated.A computational method for energy conversion during impact rock breaking was proposed,and the effects of conical tooth forward rake angle,rock temperature,and impact velocity on rock breaking characteristics and energy transfer laws were analyzed.The results show that during single impact rock breaking with conical tooth bits,merely 7.52%to 12.51%of the energy is utilized for rock breaking,while a significant 57.26%to 78.10%is dissipated as frictional loss.An insufficient forward rake angle increases tooth penetration depth and frictional loss,whereas an excessive forward rake angle reduces penetration capability,causing bit rebound and greater energy absorption by the drill rod.Thus,an optimal forward rake angle exists.Regarding environmental factors,high temperatures significantly enhance impact-induced rock breaking.Thermal damage from high temperatures reduces rock strength and inhibits its energy absorption.Finally,higher impact velocities intensify rock damage,yet excessively high velocities increase frictional loss and reduce the proportion of energy absorbed by the rock,thereby failing to substantially improve rock breaking efficiency.An optimal impact velocity exists. 展开更多
关键词 hot dry rock finite-discrete element method conical tooth bit forward rake angle impact velocity rock temperature energy transfer rock breaking mechanism
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Calculation model for kinetic energy and rock burst risk evaluation method during roadway excavation
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作者 Yunliang Tan Yan Tan +5 位作者 Weiyao Guo Bo Li Shudong He Lei Zhang Yujiang Zhang Qiuyuan Zhang 《International Journal of Mining Science and Technology》 2025年第5期677-690,共14页
The accumulation and release of deformation energy within the rock mass of a roadway are primary contributors to the occurrence of rock bursts.This study introduces a calculation model for the kinetic energy generated... The accumulation and release of deformation energy within the rock mass of a roadway are primary contributors to the occurrence of rock bursts.This study introduces a calculation model for the kinetic energy generated during roadway excavation,which is based on the fracture and energy states of the rock mass.The relationships among the mining depth,width of the plastic zone,rebound range of the roof and floor,stress concentration factor,and the induced kinetic energy are systematically explored.Furthermore,a rock burst risk evaluation method is proposed.The findings indicate that the energy evolution of the rock mass can be categorized into four stages:energy accumulation due to in-situ stress,energy accumulation resulting from coal compression,energy dissipation through coal plastic deformation,and energy consumption due to coal failure.The energy release from the rock mass is influenced by several factors,including mining depth,stress concentration factor,the width of the plastic zone,and the rebound range of the roof and floor.Within the plastic zone of coal,the energy released per unit volume of coal and the induced kinetic energy exhibit a nonlinear increase with mining depth and stress concentration factor,while they decrease linearly as the width of the plastic zone increases.Similarly,the driving energy per unit volume of the roof and floor shows a nonlinear increase with mining depth and stress concentration factor,a linear increase with the rebound range of the roof and floor,and a linear decrease with the width of the plastic zone.A rock burst risk evaluation method is developed based on the kinetic energy model.Field observations demonstrate that this method aligns with the drilling cuttings rock burst risk assessment method,thereby confirming its validity. 展开更多
关键词 Roadway rock mass Kinetic energy rock burst rock burst risk evaluation Roof and floor rebound
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Automatic identification of concealed dangerous rock blocks on highsteep slopes considering finite-sized discontinuity intersections
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作者 Xiaohan Zhao Wen Zhang +6 位作者 Junqi Chen Zhengxuan Xu Yingxu Zhang Han Yin Jia Wang Tengyue Li Bo Han 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第11期7093-7106,共14页
The identification and characterization of concealed in-situ rock blocks on high-steep slope exposures are critical in rock engineering,but remain challenging.This study employs advanced UAV-based photogrammetry to ca... The identification and characterization of concealed in-situ rock blocks on high-steep slope exposures are critical in rock engineering,but remain challenging.This study employs advanced UAV-based photogrammetry to capture high-resolution discontinuity data and develops a fully automated rock block extraction method consisting of three steps:(1)determination of free face and non-free fracture intersections,(2)surface search for rock blocks on free face.and(3)extraction and analysis of rock blocks.This approach simplifies the determination of discontinuity intersections while maintaining high accuracy.By incorporating all types of discontinuities contributing to rock block formation,the method enables precise in-situ rock block identification and extraction.Application to a rock slope in China,produced results consistent with the rock blocks observed in the 3D model,highlighting its accuracy and practical value. 展开更多
关键词 Concealed dangerous rock block High-steep rock slope rock block identification In-situ block characterization
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Experimental study on the mechanical behavior of high-stress rocks during real-time drilling
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作者 Zhichao He Fengqiang Gong Mingzhong Gao 《International Journal of Coal Science & Technology》 2025年第3期98-120,共23页
The formation of a pressure relief zone is crucial for rockbust prevention during drilling pressure relief.This study investi-gates the mechanical behavior of high-stress rock under real-time drilling conditions and e... The formation of a pressure relief zone is crucial for rockbust prevention during drilling pressure relief.This study investi-gates the mechanical behavior of high-stress rock under real-time drilling conditions and elucidates the mechanism behind the creation of the pressure relief zone.Utilizing the independently developed SG4500 drilling rig,we conducted a theoreti-cal analysis of the forces acting on the drill bit.The analysis showed that cutting depth is directly proportional to real-time drilling speed,while tangential and normal forces are influenced by drilling diameter.Uniaxial compression tests on red sandstone specimens under high-stress real-time drilling conditions examined the impacts of different drilling speeds(800,400,100 r/min)and diameters(6,8,10,12 mm)on rock mechanical behavior,rockburst characteristics,crack evolution,and peak elastic strain energy.The results indicate that decreasing drilling speed and increasing drilling diameter weaken rock mechanical behavior,including peak strength,Young's modulus,rockburst characteristics,and peak elastic strain energy.Crack evolution analysis reveals that smaller drilling diameters and higher drilling speeds promote the development of far-field cracks,while larger drilling diameters and lower drilling speeds lead to crack formation around the borehole,and significantly affecting rock failure mechanisms.Theoretical analysis further confirms the correlation between crack evolution and stress distribution surrounding the drilling.Under vertical stress,the cracks near the borehole formed during real-time drilling are mainly influenced by tangential compressive and tensile stresses.Overall,this study provides a new perspective on understanding the mechanisms of drilling pressure relief for rockburst prevention. 展开更多
关键词 rock mechanics rockBURST Drilling pressure relief Real-time drilling Crack evolution rockburst nroneness
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miR-202-5p通过靶向抑制ROCK1表达减轻急性呼吸窘迫综合征新生大鼠肺损伤
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作者 杨英阁 张晋雷 +2 位作者 孙岩 陈升 梁正 《中国医科大学学报》 北大核心 2025年第9期814-820,共7页
目的 探讨miR-202-5p对急性呼吸窘迫综合征(ARDS)新生大鼠肺损伤的保护作用及其机制。方法 60只新生SD大鼠随机分为对照组(腹腔注射生理盐水)、ARDS组[腹腔注射脂多糖(4 mg/kg)建立ARDS模型]、ARDS+miR-NC组[ARDS大鼠尾静脉注射miR-NC(2... 目的 探讨miR-202-5p对急性呼吸窘迫综合征(ARDS)新生大鼠肺损伤的保护作用及其机制。方法 60只新生SD大鼠随机分为对照组(腹腔注射生理盐水)、ARDS组[腹腔注射脂多糖(4 mg/kg)建立ARDS模型]、ARDS+miR-NC组[ARDS大鼠尾静脉注射miR-NC(2 mg/kg),1次/4 h,连续干预12 h]、ARDS+miR-202-5p组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h]、ARDS+miR-202-5p+ov-NC组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h;脂多糖注射前3 d尾静脉注射6×10~8 PFU过表达对照腺病毒]和ARDS+miR-202-5p+ov-ROCK1组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h;脂多糖注射前3 d尾静脉注射6×10^(8) PFU过表达ROCK1腺病毒],每组10只。实时定量PCR检测大鼠肺组织中miR-202-5p和ROCK1 mRNA表达;HE染色观察大鼠肺组织病理损伤;计算大鼠肺组织湿重/干重比值来评价大鼠肺水肿程度;Western blotting检测大鼠肺组织中ROCK1、TLR4、p-NF-κB p65和p-IκBα蛋白表达。双萤光素酶报告基因实验验证miR-202-5p与ROCK1的关系。结果 miR-202-5p在ARDS大鼠肺组织中表达下调,过表达miR-202-5p可减轻ARDS大鼠肺组织病理损伤和肺水肿。ROCK1 mRNA在ARDS大鼠肺组织中表达上调;过表达miR-202-5p降低ARDS大鼠肺组织中ROCK1表达,而过表达ROCK1逆转了过表达miR-202-5p对ARDS新生大鼠肺损伤的保护作用。过表达miR-202-5p抑制ARDS大鼠肺组织中TLR4、p-NF-κB p65和p-IκBα蛋白表达,过表达ROCK1逆转此作用。双萤光素酶报告基因实验结果显示miR-202-5p靶向结合ROCK1 mRNA的3’UTR。结论 miR-202-5p通过靶向抑制ROCK1表达减轻ARDS大鼠肺损伤,其机制可能与抑制ROCK1介导的TLR4/NF-κB信号通路激活有关。 展开更多
关键词 急性呼吸窘迫综合征 miR-202-5p rock1 TLR4/NF-κB信号通路 肺损伤
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Rockburst proneness criteria for rock materials:Review and new insights 被引量:61
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作者 GONG Feng-qiang WANG Yun-liang LUO Song 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第10期2793-2821,共29页
To review the rockburst proneness(or tendency)criteria of rock materials and compare the judgment accuracy of them,twenty criteria were summarized,and their judgment accuracy was evaluated and compared based on the la... To review the rockburst proneness(or tendency)criteria of rock materials and compare the judgment accuracy of them,twenty criteria were summarized,and their judgment accuracy was evaluated and compared based on the laboratory tests on fourteen types of rocks.This study begins firstly by introducing the twenty rockburst proneness criteria,and their origins,definitions,calculation methods and grading standards were summarized in detail.Subsequently,to evaluate and compare the judgment accuracy of the twenty criteria,a series of laboratory tests were carried out on fourteen types of rocks,and the rockburst proneness judgment results of the twenty criteria for the fourteen types of rocks were obtained accordingly.Moreover,to provide a unified basis for the judgment accuracy evaluation of above criteria,a classification standard(obtained according to the actual failure results and phenomena of rock specimen)of rockburst proneness in laboratory tests was introduced.The judgment results of the twenty criteria were compared with the judgment results of this classification standard.The results show that the judgment results of the criterion based on residual elastic energy(REE)index are completely consistent with the actual rockburst proneness,and the other criteria have some inconsistent situations more or less.Moreover,the REE index is based on the linear energy storage law and defined in form of a difference value and considered the whole failure process,and these superior characteristics ensure its accuracy.It is believed that the criterion based on REE index is comparatively more accurate and scientific than other criteria,and it can be recommended to be applied to judge the rockburst proneness of rock materials. 展开更多
关键词 deep rock rockBURST rockburst proneness rockburst proneness criterion rock mechanics
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Rockfall Hazard Analyses and Rockfall Protection along the Adriatic Coast of Croatia
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作者 Zeljko Arbanas Mirko Grosic +1 位作者 Dalibor Udovic Snjezana Mihalie 《Journal of Civil Engineering and Architecture》 2012年第3期344-355,共12页
During the last decade, large rockfalls occurred on the steep limestone slopes along the Adriatic Coast of Croatia, causing injury to people and serious damage to buildings and traffic facilities. The rockfalls along ... During the last decade, large rockfalls occurred on the steep limestone slopes along the Adriatic Coast of Croatia, causing injury to people and serious damage to buildings and traffic facilities. The rockfalls along the limestone slopes were caused by unfavorable characteristics of the rock mass, weathering in combination with heavy rainfall and artificial influences during highway construction. Rockfall protection projects were conducted to protect human lives and facilities from future rockfalls. The rockfall protection program started with rockfall hazard analyses to identify the potential of rockfalls to occur and the potential consequences. At the locations of hazards where related risks were determined, detailed field investigations were conducted. Based on the indentified characteristics of potentially unstable rock masses, analyses of movement and resulting pathways were conducted. The trajectories, impact energy and the height of bouncing are dependent on slope geometry, slope surface roughness and rockfall block characteristics. Two protection measure approaches were adopted: prevention of rockfalls by removing potentially unstable rock mass or installation of rock mass support systems and suspending running rockfall masses with rockfall protection barriers. In this paper, rockfall hazard determination, rockfall analyses and rockfall protection designs for rockfall protection systems at selected locations on the limestone slopes along the Adriatic coast of Croatia are presented. 展开更多
关键词 rockFALL rockfall hazard rockfall hazard analyses rockfall protection measures rockfall protection barriers rock masss upport.
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Experiments on image data augmentation techniques for geological rock type classification with convolutional neural networks 被引量:2
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作者 Afshin Tatar Manouchehr Haghighi Abbas Zeinijahromi 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第1期106-125,共20页
The integration of image analysis through deep learning(DL)into rock classification represents a significant leap forward in geological research.While traditional methods remain invaluable for their expertise and hist... The integration of image analysis through deep learning(DL)into rock classification represents a significant leap forward in geological research.While traditional methods remain invaluable for their expertise and historical context,DL offers a powerful complement by enhancing the speed,objectivity,and precision of the classification process.This research explores the significance of image data augmentation techniques in optimizing the performance of convolutional neural networks(CNNs)for geological image analysis,particularly in the classification of igneous,metamorphic,and sedimentary rock types from rock thin section(RTS)images.This study primarily focuses on classic image augmentation techniques and evaluates their impact on model accuracy and precision.Results demonstrate that augmentation techniques like Equalize significantly enhance the model's classification capabilities,achieving an F1-Score of 0.9869 for igneous rocks,0.9884 for metamorphic rocks,and 0.9929 for sedimentary rocks,representing improvements compared to the baseline original results.Moreover,the weighted average F1-Score across all classes and techniques is 0.9886,indicating an enhancement.Conversely,methods like Distort lead to decreased accuracy and F1-Score,with an F1-Score of 0.949 for igneous rocks,0.954 for metamorphic rocks,and 0.9416 for sedimentary rocks,exacerbating the performance compared to the baseline.The study underscores the practicality of image data augmentation in geological image classification and advocates for the adoption of DL methods in this domain for automation and improved results.The findings of this study can benefit various fields,including remote sensing,mineral exploration,and environmental monitoring,by enhancing the accuracy of geological image analysis both for scientific research and industrial applications. 展开更多
关键词 Deep learning(DL) Image analysis Image data augmentation Convolutional neural networks(CNNs) Geological image analysis rock classification rock thin section(RTS)images
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基于RhoA/ROCK通路研究复方中药对腹水肉鸡心肌损伤的作用
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作者 康杰 王慧敏 +1 位作者 邓瑞强 段智变 《特产研究》 2025年第3期44-48,54,共6页
为分析RhoA/ROCK信号通路在肉鸡腹水综合征(Ascites syndrome, AS)心肌损伤中的作用及复方中药的防治机制,本研究选取100羽健康的罗斯肉鸡(7日龄)并随机分成5组(每组20只),即基础饲粮饲养对照组,多因素法构建的AS模型组,复方中药高、低... 为分析RhoA/ROCK信号通路在肉鸡腹水综合征(Ascites syndrome, AS)心肌损伤中的作用及复方中药的防治机制,本研究选取100羽健康的罗斯肉鸡(7日龄)并随机分成5组(每组20只),即基础饲粮饲养对照组,多因素法构建的AS模型组,复方中药高、低剂量治疗组以及L-精氨酸治疗组。观察临床症状和剖检变化,并通过HE染色和Masson染色分别观察35日龄肉鸡心脏组织结构变化和胶原纤维沉积;计算肉鸡腹水心脏指数(Ascites heart index, AHI);利用实时荧光定量PCR检测心脏组织MYPT1 mRNA的转录情况,ELISA法检测心脏组织RhoA/ROCK信号通路相关蛋白的表达。结果表明,与空白对照组相比,模型组肉鸡腹部膨大,触压有波动感,腹腔内呈现大量淡黄色液体,右心肥大,心脏组织结构排列紊乱,胶原纤维大量沉积,而且M组AHI显著升高(P <0.01);荧光定量PCR结果表明,心脏组织MYPT1 mRNA转录显著升高(P <0.01)。ELISA结果表明,RhoA、ROCK、P-MLC表达显著升高(P <0.01),而MLCP表达显著降低(P <0.01);与AS组相比,复方中药治疗组的肉鸡相关临床症状及上述指标明显改善。本研究发现,RhoA/ROCK通路介导的MLC磷酸化参与了AS中心肌损伤的发生,复方中药水提液可通过调节RhoA/ROCK抑制心肌损伤来有效防治AS。 展开更多
关键词 复方中药 肉鸡腹水综合征 心肌损伤 RhoA/rock通路
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基于RhoA/ROCK通路探讨多囊卵巢综合征的发病机制与中医药干预研究进展
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作者 陈茜 郑承红 +1 位作者 汪新妮 丑丹 《中南药学》 2025年第11期3305-3312,共8页
多囊卵巢综合征(PCOS)是一种常见的妇科内分泌紊乱疾病,影响女性身心健康。中医药具有多靶点、多通路、全身调理等特点,在防治PCOS中有显著的临床疗效,但其分子机制尚未明确。近年来,Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(... 多囊卵巢综合征(PCOS)是一种常见的妇科内分泌紊乱疾病,影响女性身心健康。中医药具有多靶点、多通路、全身调理等特点,在防治PCOS中有显著的临床疗效,但其分子机制尚未明确。近年来,Ras同源基因家族成员A(RhoA)/Rho相关的卷曲螺旋激酶(ROCK)通路在PCOS病理生理过程中的作用逐渐受到关注。本文综述了RhoA/ROCK通路的组成与基本功能,讲述其在PCOS相关的卵巢功能异常、胰岛素抵抗、慢性炎症和血管功能障碍等方面的研究进展。并总结目前中药单体、复方及中医外治法等通过调控RhoA/ROCK通路治疗PCOS的研究进展。旨在为深入理解PCOS的发病机制以及开发新的治疗策略提供参考。 展开更多
关键词 多囊卵巢综合征 RhoA/rock通路 发病机制 中医药
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Time effect of elastic energy release of surrounding rock and evaluation method of reasonable advancing speed 被引量:1
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作者 Ruifu Yuan Qi Ma +3 位作者 Qunlei Zhang Chun Feng Chunfu Wei Yan Gao 《International Journal of Coal Science & Technology》 2025年第1期113-124,共12页
To reveal the rock burst mechanism,the stress and failure characteristics of coal-rock strata under different advancing speeds of mining working face were explored by theoretical analysis,simulation,and engineering mo... To reveal the rock burst mechanism,the stress and failure characteristics of coal-rock strata under different advancing speeds of mining working face were explored by theoretical analysis,simulation,and engineering monitoring.The relationship between energy accumulation and release was analyzed,and a reasonable mining speed according to specific projects was recommended.The theoretical analysis shows that as the mining speed increases from 4 to 15 m/d,the rheological coefficient of coal mass ranges from 0.9 to 0.4,and the elastic energy of coal mass accumulation varies from 100 to 900 kJ.Based on the simulation,there is a critical advancing speed,the iteration numbers of simulation are less than 15,000 per mining 10 m coal seam,the overburden structure is obvious,the abutment pressure in coal mass is large,and the accumulated energy is large,which is easy to cause strong rock burst.When the iteration number is greater than 15,000,the static force of coal mass increases slightly,but there is no obvious rock burst.Based on engineering monitoring,the mining speed of a mine is less than 8 m/d,and the periodic weighting distance is about 17 m;as the mining speed is greater than 10 m/d,and the periodic weighting distance is greater than 20 m;as the mining speed is 3-8 m/d,and the range of high stress in surrounding rock is 48 m;as the advancing speed is 8-12 m/d,and the high-stress range in surrounding rock is 80 m.Moreover,as the mining speed is less than 8 cut cycles,the micro seismic energy is less than 10,000 J;as the mining speed is 12 cut cycles,the microseismic energy is about 20,000 J.In summary,the advancing speed is positively correlated with the micro seismic event;as the mining speed increases,the accumulated elastic energy of surrounding rock is greater,which is easy to cause rock burst.The comprehensive analysis indicates the daily advance speed of the mine is not more than 12 cut cycles. 展开更多
关键词 Mining speed rock burst Engineering analysis Simulation Coal-rock stress
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Study on the variation of the permeability coefficient of soil-rock mixtures in fault zones under different stress states 被引量:1
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作者 Wenhui Tan Shuang Liang +1 位作者 Xuewen Ma Pengfei Wang 《Deep Underground Science and Engineering》 2025年第2期210-221,共12页
As the first gold mine discovered at the sea in China and the only coastal gold mine currently mined there,Sanshandao Gold Mine faces unique challenges.The mine's safety is under continual threat from its faulted ... As the first gold mine discovered at the sea in China and the only coastal gold mine currently mined there,Sanshandao Gold Mine faces unique challenges.The mine's safety is under continual threat from its faulted structure coupled with the overlying water.As the mining proceeds deeper,the risk of water inrush increases.The mine's maximum water yield reaches 15000 m3/day,which is attributable to water channels present in fault zones.Predominantly composed of soil–rock mixtures(SRM),these fault zones'seepage characteristics significantly impact water inrush risk.Consequently,investigating the seepage characteristics of SRM is of paramount importance.However,the existing literature mostly concentrates on a single stress state.Therefore,this study examined the characteristics of the permeability coefficient under three distinct stress states:osmotic,osmotic–uniaxial,and osmotic–triaxial pressure.The SRM samples utilized in this study were extracted from in situ fault zones and then reshaped in the laboratory.In addition,the micromechanical properties of the SRM samples were analyzed using computed tomography scanning.The findings reveal that the permeability coefficient is the highest under osmotic pressure and lowest under osmotic–triaxial pressure.The sensitivity coefficient shows a higher value when the rock block percentage ranges between 30%and 40%,but it falls below 1.0 when this percentage exceeds 50%under no confining pressure.Notably,rock block percentages of 40%and 60%represent the two peak points of the sensitivity coefficient under osmotic–triaxial pressure.However,SRM samples with a 40%rock block percentage consistently show the lowest permeability coefficient under all stress states.This study establishes that a power function can model the relationship between the permeability coefficient and osmotic pressure,while its relationship with axial pressure can be described using an exponential function.These insights are invaluable for developing water inrush prevention and control strategies in mining environments. 展开更多
关键词 permeability coefficient rock block percentage sensitivity analysis soil-rock mixture stress state
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酸浆苦素B通过抑制RhoA/ROCK信号通路抑制结直肠癌细胞增殖侵袭和迁移并诱导凋亡
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作者 胡秀花 谭福勇 《中国组织化学与细胞化学杂志》 2025年第4期341-348,共8页
目的 探究酸浆苦素B(physalin B,PB)对结直肠癌(CRC)细胞恶性生物学行为的影响,分析其机制是否与Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路有关。方法 选用CRC细胞SW480,在含不同浓度PB(5、10、20μmol/L)... 目的 探究酸浆苦素B(physalin B,PB)对结直肠癌(CRC)细胞恶性生物学行为的影响,分析其机制是否与Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋形成蛋白激酶(ROCK)信号通路有关。方法 选用CRC细胞SW480,在含不同浓度PB(5、10、20μmol/L)或PB+Rho/ROCK信号通路激动剂溶血磷脂酸(lysophosphatidic acid,LPA)(20μmol/L PB+50μmol/L LPA)的处理条件下,采用CCK-8法和Edu染色检测细胞增殖;利用流式细胞术和TUNEL染色;检测细胞凋亡;通过Transwell实验和划痕实验;检测细胞侵袭和迁移能力;采用Western blot;检测RhoA、ROCK及凋亡相关蛋白(Bcl-2、Bax、caspase-3)的表达水平。结果 CCK-8和Edu染色检测显示,细胞增殖能力呈PB(5、10、20μmol/L)处理浓度依赖性下降。PB处理后,Transwell和划痕实验显示细胞侵袭与迁移能力下降,流式细胞术与TUNEL染色结果显示细胞凋亡率升高;Western blot检测发现Bax和caspase-3蛋白表达增加,Bcl-2、RhoA和ROCK蛋白表达减少。在含PB+LPA(20μmol/L PB+50μmol/L LPA)处理条件下,细胞增殖、侵袭与迁移的下降,凋亡率的增加,Bcl-2、RhoA和ROCK蛋白表达的降低,以及Bax和Caspase-3蛋白表达的下调明显减少。结论 PB可能通过抑制RhoA/ROCK信号通路,抑制CRC细胞增殖、侵袭和迁移等恶性生物学行为,诱导CRC细胞凋亡,进而发挥抑制CRC的作用。 展开更多
关键词 酸浆苦素B RhoA/rock信号通路 结直肠癌 增殖 凋亡
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Effects of bedding angles on rockburst proneness of layered anisotropic phyllites 被引量:1
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作者 Lei Xu Fengqiang Gong +1 位作者 Jinhao Dai Zhichao He 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第7期4288-4313,共26页
To examine the effect of bedding angle upon burst proneness in terms of energy,phyllites with seven various bedding angles are selected for conventional uniaxial compression and single-cyclic loading eunloading uniaxi... To examine the effect of bedding angle upon burst proneness in terms of energy,phyllites with seven various bedding angles are selected for conventional uniaxial compression and single-cyclic loading eunloading uniaxial compression tests.The ejection and failure during compression process of phyllites are monitored in real-time by high-speed camera system.The results demonstrate that the phyllites with different bedding angles all consistently follow the linear energy storage and dissipation(LESD)law during compression.The ultimate energy storage of phyllites with varying bedding angles can be calculated precisely via using the LESD law.Based on this,four kinds of energy-based rockburst indices are applied to quantitatively assess the burst proneness for phyllites.Combined with the recorded images of high-speed camera system,ejection distance,and mass of rock fragments and powder,the burst proneness for phyllites with various bedding angles is qualitatively evaluated adopting the far-field ejection mass ratio.Next,burst proneness of anisotropic phyllites is assessed quantitatively and qualitatively.It is found that phyllites with bedding angles of 0°,15°,and 90°have a high burst proneness,and that with bedding angle of 30°has a medium burst proneness,whereas the ones with bedding angles of 45°,60°,and 75°have a low burst proneness.Finally,the published experimental data of shale and sandstone specimens with different bedding angles are extracted,and it is preliminarily verified that the bedding angle does not change the LESD law of rocks. 展开更多
关键词 rock mechanics Bedding angle rockburst proneness Linear energy storage and dissipation law Residual elastic energy
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毛兰素通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎 被引量:1
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作者 徐可欣 王丹丹 +5 位作者 金泓宇 杜月 李莉 宋艺兰 延光海 李良昌 《中国比较医学杂志》 北大核心 2025年第4期11-20,共10页
目的探究毛兰素(Erianin)在特应性皮炎(atopic dermatitis,AD)中的作用及其在高迁移率族蛋白1(high mobility group box-1,HMGB1)/晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)-Ras同源基因家族成员A(Ra... 目的探究毛兰素(Erianin)在特应性皮炎(atopic dermatitis,AD)中的作用及其在高迁移率族蛋白1(high mobility group box-1,HMGB1)/晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)-Ras同源基因家族成员A(Ras homolog gene family member A,RhoA)/Rho关联含卷曲螺旋结合蛋白激酶1(recombinant Rho associated coiled coil containing protein kinase 1,ROCK1)信号通路中的调控机制。方法1-氯-2,4-二硝基苯(1-Chloro-2,4-dinitrobenzene,DNCB)诱导BALB/c小鼠作为AD的模型,测量小鼠的皮肤厚度、脾和淋巴结的重量。甲苯胺蓝和HE染色检测小鼠的背部皮肤和耳朵的病理改变;ELISA检测炎症因子水平;肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)刺激HaCaT细胞建立AD体外模型;采用流式细胞术检测细胞活性氧(reactive oxygen species,ROS);免疫荧光法检测线粒体活性氧(mitochondrion reactive oxygen species,mtROS);TUNEL检测细胞凋亡情况;免疫蛋白印迹法检测HMGB1、RAGE、RhoA、ROCK1蛋白表达情况。结果在体内实验中毛兰素抑制皮肤厚度的增加,减轻脾和淋巴结重量,改善炎症细胞的浸润和肥大细胞脱颗粒,降低炎症因子水平(P<0.05)。在体外实验中,毛兰素减少TNF-α诱导的HaCaT细胞ROS、mtROS的产生(P<0.01)。毛兰素治疗后HMGB1、RAGE、RhoA及ROCK1的蛋白表达量下降(P<0.01);使用RAGE特异性阻断剂(TFA)处理r-HMGB1刺激的HaCaT细胞后,HMGB1的表达没有发生变化,RAGE、RhoA及ROCK1表达减少(P<0.01);在Rho激酶抑制剂Y-27632+r-HMGB1组中,除RAGE的表达没有降低,其余结果与TFA+r-HMGB1组相近。结论毛兰素可能通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎。 展开更多
关键词 毛兰素 特应性皮炎 HMGB1 RAGE RHOA rock1
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Effects of elevated ground temperatures on properties of cement grouts for deep rock grouting
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作者 Zhipeng Xu Jianping Sun +2 位作者 Runguo Li Lei He Changwu Liu 《Deep Underground Science and Engineering》 2025年第2期171-188,共18页
Appropriate determination of the mix ratios of cement grouts is of vital importance to the quality of rock grouting and the risk reduction of groundwater inflow.The behavior of grout,often highly temperature dependent... Appropriate determination of the mix ratios of cement grouts is of vital importance to the quality of rock grouting and the risk reduction of groundwater inflow.The behavior of grout,often highly temperature dependent,is likely to be affected by the elevated ground temperature in deep rock masses.This paper aims to experimentally gain insights into the effects of elevated ground temperatures on the properties of cement grout in fresh and hardened states in deep rock grouting.The results revealed that a temperature of 35°C is crucial for changes in the properties of thick cement grout with a water–cement ratio of less than 0.8.When the temperature is up to 35°C,there can be significant improvements in rheological parameters,acceleration of grout setting,and increase in the rheological time dependence of thick cement grout;however,there may also be a slight impact on the initial grout flowability and the nature of shear thinning.The high temperature may still improve the stability of fresh cement grout and also improve the porosity and creep deformation of hardened cement grout considerably.The proposed constitutive model that couples the Burgers model with a fractional derivativebased Abel dashpot in the series can be used to characterize the creep behavior of hardened cement grout appropriately.The paper provides a valuable reference for optimization of mixture design of cement grouts,thus enhancing deep rock grouting quality and improving safety. 展开更多
关键词 cement grout CREEP deep rock RHEOLOGY rock grouting temperature
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Anisotropy of non-Darcian flow in rock fractures subjected to cyclic shearing
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作者 Zihao Sun Liangchao Zou +3 位作者 Liangqing Wang Jia-Qing Zhou Zhen Cui Qiong Wu 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第10期6373-6387,共15页
Non-Darcian flow in rock fractures exhibits significant anisotropic characteristics,which can be affected by mechanical processes,such as cyclic shearing.Understanding the evolution of anisotropic nonDarcian flow is c... Non-Darcian flow in rock fractures exhibits significant anisotropic characteristics,which can be affected by mechanical processes,such as cyclic shearing.Understanding the evolution of anisotropic nonDarcian flow is crucial for characterizing groundwater flow and mass/heat transport in fractured rock masses.In this study,we conducted experiments on non-Darcian flow in single rough fractures under cyclic shearing conditions,aiming to analyze the anisotropic evolution of inertial permeability and viscous permeability.We established quantitative characterization models for the two types of permeability.First,we conducted cyclic shearing experiments on four sets of 24 rough rock fractures,investigating their shear characteristics.Then,we performed 480 non-Darcian flow experiments to analyze the anisotropic evolution of viscous permeability and inertial permeability of these rock fractures.The results showed that viscous permeability exhibited significant differences only in the orthogonal direction,while inertial permeability exhibited differences in both orthogonal and opposite directions.With increase in the shear cycles,the differences in the orthogonal direction gradually increased,while those in opposite direction gradually decreased.Finally,we established characterization equations for the two permeabilities based on the proposed directional geometric parameters and validated the performance of these equations with experimental data.These findings are useful for the quantitative characterization of the evolution of non-Darcian flow in fractures under dynamic loading conditions. 展开更多
关键词 rock fractures Cyclic shearing Viscous permeability Inertial permeability rock anisotropy Non-darcian flow
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