为检测贵州省苗族新生儿血红蛋白中的A T 变异体 ,以便进一步分析变异个体的基因型 ,首先必须用适当的方法检测出新生儿脐带血HbF中的A T 链。介绍用反相高效液相层析技术 (RHPLC)检测人类HbF中的A T 链 ,所用主要试剂乙腈为国内产品。...为检测贵州省苗族新生儿血红蛋白中的A T 变异体 ,以便进一步分析变异个体的基因型 ,首先必须用适当的方法检测出新生儿脐带血HbF中的A T 链。介绍用反相高效液相层析技术 (RHPLC)检测人类HbF中的A T 链 ,所用主要试剂乙腈为国内产品。该检测方法具有程序化、自动化、灵敏度高、快速、准确的特点。该方法的建立为A T的检测及基因表达产物的鉴定 ,提供了新的。展开更多
AIM:To detect the plateletoactivating factor(PAF)and the plasma or serum levels of tumor necrosis factoroa(TNF-α)malondialdehyde(MDA),endotoxin(ET)and to discuss their significance in various types of viral hepatitis...AIM:To detect the plateletoactivating factor(PAF)and the plasma or serum levels of tumor necrosis factoroa(TNF-α)malondialdehyde(MDA),endotoxin(ET)and to discuss their significance in various types of viral hepatitis.METHODS:PAF,TNF-α,MDA,and ET levels in 60 controls,16 cases of acute viral hepatitis,71 cases of chronic viral hepatitis,19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography(rHPLC),bio-assay,ELISA,thiobarbituric acid(TBA),and limulus lysate test(LLT),respectively.RESULTS:The rHPLC was more sensitive and specific than bio-assay(r=0.912,P〈0.01).The plasma levels of PAF,TNF-α,MDA,and ET in patients with viral hepatitis were higher than those in controls(P〈0.01).CONCLUSION:rHPLC is more reliable and accurate for the detection of PAF.展开更多
文摘为检测贵州省苗族新生儿血红蛋白中的A T 变异体 ,以便进一步分析变异个体的基因型 ,首先必须用适当的方法检测出新生儿脐带血HbF中的A T 链。介绍用反相高效液相层析技术 (RHPLC)检测人类HbF中的A T 链 ,所用主要试剂乙腈为国内产品。该检测方法具有程序化、自动化、灵敏度高、快速、准确的特点。该方法的建立为A T的检测及基因表达产物的鉴定 ,提供了新的。
基金Supported by a Health Foundation of Zhejiang Province,No.2004C083,2004B068
文摘AIM:To detect the plateletoactivating factor(PAF)and the plasma or serum levels of tumor necrosis factoroa(TNF-α)malondialdehyde(MDA),endotoxin(ET)and to discuss their significance in various types of viral hepatitis.METHODS:PAF,TNF-α,MDA,and ET levels in 60 controls,16 cases of acute viral hepatitis,71 cases of chronic viral hepatitis,19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography(rHPLC),bio-assay,ELISA,thiobarbituric acid(TBA),and limulus lysate test(LLT),respectively.RESULTS:The rHPLC was more sensitive and specific than bio-assay(r=0.912,P〈0.01).The plasma levels of PAF,TNF-α,MDA,and ET in patients with viral hepatitis were higher than those in controls(P〈0.01).CONCLUSION:rHPLC is more reliable and accurate for the detection of PAF.
