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Effect of no-load rate on recast layer cutting by ultra fine wire-EDM 被引量:1
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作者 Ming ZHANG Zhidong LIU +2 位作者 Hongwei PAN Cong DENG Mingbo QIU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2021年第4期124-131,共8页
Via material erosion in wire electrical discharge machining(WEDM),recast layers form on the surfaces of workpiece.In addition,ultra fine Wire-EDM can be usually cut once.To reduce the thickness of the recast layer as ... Via material erosion in wire electrical discharge machining(WEDM),recast layers form on the surfaces of workpiece.In addition,ultra fine Wire-EDM can be usually cut once.To reduce the thickness of the recast layer as much as possible,the wire electrical discharge-electrochemical machining(WEDCM)method was proposed,which is based on the micro conductivity of the dielectric and microelectrolytic characteristics by adjusting the no-load rate of the pulse in the machining process.Furthermore,a state discrimination and servo control system based on discharge current was designed.The experiment results of different no-load rates show that the electrolytic effects increase as the no-load rate increases,and the main machining process is spark discharge erosion with a no-load rate in the range of 10%to 80%.At 90%no-load rate,the amount of recast layer formation in the forward direction of the wire electrode is almost the same as that of electrolytic dissolution,and it can be practically processed without a recast layer.Compared with10%no-load rate,the kerf width only increases by 7.5%. 展开更多
关键词 Electrolytic dissolution coefficient No-load rate recast layer Ultra fine wire WEDCM
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The Impact of Recasts on Second Language Acquisition
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作者 郭小华 《海外英语》 2011年第8X期172-173,共2页
The roles of recasts have been intensively studied in the first language (L1) research. This essay explores the role of recasts in second language acquisition, mainly focusing on discussing the studies addressing the ... The roles of recasts have been intensively studied in the first language (L1) research. This essay explores the role of recasts in second language acquisition, mainly focusing on discussing the studies addressing the following two issues: (1) the effects of recasts on the interaction of communication oriented classroom; (2) the role of recasts in promoting interlanguage development.Mixed evidence has been presented by second language (L2) studies regarding the impacts of recasts on second language acquisition. 展开更多
关键词 recastS communicative-oriented CLASSROOM SECOND LANGUAGE ACQUISITION
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Is Recast the Most Effective Type of Corrective Feedback? --Under Cognitive and Sociolinguistic Approach
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作者 GU Bing-jie 《Sino-US English Teaching》 2016年第4期284-291,共8页
Currently, there are a variety of researches on corrective feedback, especially on recasts. Most of researches are experiment-based and depend on the certain context. This paper addresses the question of "Is recast t... Currently, there are a variety of researches on corrective feedback, especially on recasts. Most of researches are experiment-based and depend on the certain context. This paper addresses the question of "Is recast the most effective form of corrective feedback?" by two of Second Language Acquisition (SLA) approaches: cognitive approach and sociolinguistic approach. Empirical research will be used as complement. Theoretically speaking, SLA hypotheses in cognitive approach exist disagreements. Interaction hypothesis favors recast while both input hypothesis and output hypothesis doubt the effectiveness of it. Sociolinguistic approach underscores the significance of appropriate and timely corrective feedback and does not give more credit to certain strategy. Both approaches provide the pedagogical implications and elements to take into account for adopting recasts. 展开更多
关键词 recastS corrective feedback cognitive approach sociolinguistic approach
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A Brief Review on Recasts
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作者 于澎涛 《海外英语》 2014年第15期260-261,共2页
Recast is the most frequently used error correction technique adopted by teachers in the communicative oriented classrooms. This article illustrates the reasons for its popularity, also discusses how to use recast eff... Recast is the most frequently used error correction technique adopted by teachers in the communicative oriented classrooms. This article illustrates the reasons for its popularity, also discusses how to use recast effectively in practice. 展开更多
关键词 recastS COMMUNICATIVE LANGUAGE teaching(CLT) FOCUS
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How effective are recasts in facilitating second language acquisition?
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作者 蔡维效 《和田师范专科学校学报》 2009年第6期138-139,共2页
Recasts are the most frequently used in class to give corrective feedback.However,there is a hot debate about their effectiveness in facilitating second language acquisition(SLA).The article aims to examine the effect... Recasts are the most frequently used in class to give corrective feedback.However,there is a hot debate about their effectiveness in facilitating second language acquisition(SLA).The article aims to examine the effectiveness and explore ways to optimize the facilitative role of recasts in class. 展开更多
关键词 外语学习 英语学习 语言学 师范院校
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Effect of Surface Treatment on Elemental Composition of Recast NiCr Alloy
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作者 Tevfik Yavuz Asli Acar +1 位作者 Serhan Akman Atiye Nilgun Ozturk 《Materials Sciences and Applications》 2012年第3期163-167,共5页
Objective: The aim of this study was to compare the effects of different surface treatments on the elemental composition stability of as-received and recast types of NiCr casting alloys. Material and Methods: Six comm... Objective: The aim of this study was to compare the effects of different surface treatments on the elemental composition stability of as-received and recast types of NiCr casting alloys. Material and Methods: Six commercial NiCr dental casting alloys (Kera N, Nodelco, Bellabond, Wiron 99, Metaplus VK, and Tritech D) were used in this study. Seventy-two specimens (12 × 12 × 1.2 mm) were produced using the lost-wax casting process. Three casting protocols were established in relation to the proportion of as-received and recast alloys: Group A (100% as-received metal), Group B (50 wt% new metal, 50 wt% once-recast metal), and Group C (100% once-recast metal). Two specimens in every group received two different surface treatments: sandblasting with 110 μmm Al2O3 or oxidation firing. An elemental analysis was performed with x-ray energy-dispersive spectroscopy. Results were subjected to one-way analysis of variance and Tukey HSD tests. Results: The elemental composition was significantly different in the casting groups and treatment groups (p < 0.05). The lowest mean weight percentage Ni value was recorded for Group C and the highest for Group A. Aluminum-oxide sandblasting of the alloy surface reduced the mean weight percentage for Cr. Conclusion: Recasting metal alloys may adversely affect surface quality. 展开更多
关键词 recasting Elemental Composition NiCr Alloy
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Explicit Recast, Implicit Recast and the Acquisition of English Noun Plural: A Comparative Study 被引量:1
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作者 陈卓 《Chinese Journal of Applied Linguistics》 2010年第6期55-70,127,共17页
该研究对比分析了显性重述和隐性重述对中国英语学习者英语名词复数习得的影响。63名小学六年级学生被随机分配到显性重述组、隐性重述组和控制组。每组21人。本研究的目标语法结构为英语名词复数。受试者单独同研究者完成了实验干预—... 该研究对比分析了显性重述和隐性重述对中国英语学习者英语名词复数习得的影响。63名小学六年级学生被随机分配到显性重述组、隐性重述组和控制组。每组21人。本研究的目标语法结构为英语名词复数。受试者单独同研究者完成了实验干预—信息差型任务。针对受试者产生的错误目标结构,显性重述组得到显性重述作为反馈,隐性重述组得到隐性重述作为反馈。而控制组得不到任何纠错型反馈。受试者关于目标结构的知识分别进行了前测、后测、延时后测。配对样本t检验和单因素方差分析的结果表明:三组的后测成绩都比前测成绩有显著性的提高;显性重述组的两次后测成绩都比隐性重述和控制组好;隐性重述组同控制组之间没有显著性差异。本文根据注意假设、自发诱导理论和平衡假设讨论了实验结果。在理论上,显性重述相对于隐性重述的优势证实了负面信息在二语习得中的有益作用。在教学上,其说明显性重述比隐性重述更适用于二语课堂。 展开更多
关键词 explicit recast implicit recast grammar development effect
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RECASTS AS CORRECTIVE FEEDBACK IN CHINA’S ENGLISH CLASSROOM INTERACTION
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作者 赵晨 《Chinese Journal of Applied Linguistics》 2008年第3期30-34,127,共6页
本文基于30节课堂教学语料调查了中国英语教师使用部分重说反馈语(recast)的频率和规律。数据表明,中国英语教师并不倾向在课堂中频繁使用部分重说;而当他们使用时,他们一般针对学生的语法错误使用部分重说;并且和其它反馈语相比,部分... 本文基于30节课堂教学语料调查了中国英语教师使用部分重说反馈语(recast)的频率和规律。数据表明,中国英语教师并不倾向在课堂中频繁使用部分重说;而当他们使用时,他们一般针对学生的语法错误使用部分重说;并且和其它反馈语相比,部分重说不能导致学生产出更多的即时修正。原因可能与部分重说的性质和教师使用它的技巧有关。 展开更多
关键词 recast corrective feedback classroom interaction
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Second Language Research on Recasts:A Critical Review in Response to an Ongoing Debate 被引量:2
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作者 杨承松 胡光伟 张军 《Chinese Journal of Applied Linguistics》 2014年第4期411-429,539,共20页
This paper reviews important second language research on recasts in response to a recent debate between Goo and Mackey (2013) and Lyster and Ranta (2013) in the journal Studies in Second Language Acquisition. It b... This paper reviews important second language research on recasts in response to a recent debate between Goo and Mackey (2013) and Lyster and Ranta (2013) in the journal Studies in Second Language Acquisition. It begins by clarifying the definition of recasts and then examines several controversial issues featuring prominently in the debate, including the theoretical underpinnings of existing research on recasts, mixed findings about their effectiveness in language acquisition, and methodological concerns in effectiveness research. The primary aim of this paper is to demarcate common ground and disagreement that are emerging in this debate and, based on a synthesis and critique of previous empirical studies, to identify directions for future research. It also explores pedagogical implications of recasts research for English-as-a-foreign-language contexts. 展开更多
关键词 recastS corrective feedback INTERACTIONISM second language acquisition
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The Effects of Recasts and Working Memory on the Development of Implicit and Explicit Knowledge 被引量:1
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作者 Jieun AHN Youngkyu KIM 《Chinese Journal of Applied Linguistics》 2016年第1期3-25,132,共24页
This paper aims to investigate the effects of recasts and working memory on the acquisition of Korean morphological causatives by advanced Chinese learners of Korean. Participants were randomly assigned to two groups... This paper aims to investigate the effects of recasts and working memory on the acquisition of Korean morphological causatives by advanced Chinese learners of Korean. Participants were randomly assigned to two groups: A experimental group and a control group. The experimental group received intensive recasts during task-based interaction, whereas the control group did not. The effects of recasts were measured by two types of tests: An elicited imitation test, as a measure of implicit knowledge, and an untimed grammaticality judgment test, as a measure of explicit knowledge. The findings are as follows. First, from the pretest to the delayed posttest, recasts facilitated the learners' acquisition of causative construction by developing both their implicit and explicit knowledge. Second, the results of delayed posttests showed that recasts were more effective in aiding the development of implicit knowledge than explicit knowledge. Third, working memory was found to be a significant covariate in the facilitative effects of recasts on explicit knowledge; that is, it mediated the development of explicit knowledge via recasts as a significant individual difference factor. The effects of recasts were maintained even when the impact of working memory was controlled. (189 words) 展开更多
关键词 corrective feedback recastS working memory implicit knowledge explicit knowledge Korean as a second language Chinese learners of Korean
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Recasting Anti-commercial Bribery Provisions in Criminal Law
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作者 曾友祥 《Social Sciences in China》 2012年第1期92-103,共12页
Commercial bribery makes up a high proportion of all corruption involving public functionaries in China. As such, it must be a present and future focus of the fight against corruption. In order to better achieve the o... Commercial bribery makes up a high proportion of all corruption involving public functionaries in China. As such, it must be a present and future focus of the fight against corruption. In order to better achieve the objective of restraining public power, we should adjust the criteria for what constitutes the crime of corruption and the content of the specific assessment of this crime in Chinese criminal law in the light of the characteristics and new manifestations of commercial bribery, with a view to combating it more effectively.View full textDownload full text 展开更多
关键词 commercial bribery criminal regulations recasting of mechanisms
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电火花线切割Inconel 718温度场分析与加工建模
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作者 张耀满 吴双金 饶兆峰 《东北大学学报(自然科学版)》 北大核心 2025年第3期88-96,共9页
针对Inconel 718材料的高加工硬化率和切削温度变化大等特点,以电火花线切割(WEDM)加工Inconel 718的放电加工过程为研究对象,对其加工机理和建模进行深入研究.采用有限差分法和有限元仿真对单脉冲放电温度场进行分析,得到了给定参数下... 针对Inconel 718材料的高加工硬化率和切削温度变化大等特点,以电火花线切割(WEDM)加工Inconel 718的放电加工过程为研究对象,对其加工机理和建模进行深入研究.采用有限差分法和有限元仿真对单脉冲放电温度场进行分析,得到了给定参数下的理论、仿真温度分布结果,并进一步探究了脉冲宽度对电蚀坑尺寸与形状的影响规律.在考虑重铸层对电蚀坑尺寸影响的基础上,预测了加工的表面粗糙度和材料去除率,并与实验数据进行对比.结果表明:随着脉冲宽度的变化,理论与仿真的电蚀坑尺寸变化趋势一致,所建立工艺目标预测模型的理论和仿真数据与实验结果的最大误差为9.88%. 展开更多
关键词 电火花线切割 Inconel 718 有限差分法 有限元法 温度场分析 重铸层
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Research on the Microstructure Characterization and Fatigue Behavior of Nickel-Based Superalloy Subjected to Short-Arc and Milling Composite Processing
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作者 Pai Wang Wenxiang Zhao +4 位作者 Xibin Wang Shuyao Liu Yifan Bai Hongtao Chen Zhibing Liu 《Chinese Journal of Mechanical Engineering》 2025年第6期427-438,共12页
Short-arc machining is a novel electrical discharge machining method that utilizes high-energy arc discharge as the energy carrier.Due to its low cost and high processing efficiency,it has been widely applied in the e... Short-arc machining is a novel electrical discharge machining method that utilizes high-energy arc discharge as the energy carrier.Due to its low cost and high processing efficiency,it has been widely applied in the efficient processing of superalloys.To address the challenges of efficient and high-precision processing of superalloys,a processing method combining short-arc machining with precision milling is employed.Advanced material characterization techniques such as electron backscatter diffraction(EBSD)are utilized to analyze the physical properties of the recast layer and surface crystal characteristics.High-temperature low-cycle fatigue life tests are conducted to investigate the correlation between fatigue life and typical surface integrity parameters(surface roughness,residual stress),as well as crystallographic parameters(grain size,grain orientation spread,geometrically necessary dislocations).Processing parameter optimization is achieved with fatigue life as the target.The results indicate that at high temperatures during short-arc machining,the surface material underwent recrystallisation to form a recast layer with a grain size reduction of 85.5%and a heat affected layer depth of over 400μm.The trends in fatigue life are consistent with changes in residual stress,grain orientation spread and geometrically necessary dislocations.Selecting a larger axial depth of cut and lower feed per tooth is advantageous for achieving a higher fatigue life.The proposed research provides an instruction for high efficient precision machining of superalloys. 展开更多
关键词 GH4169 superalloy Short-arc machining recast layer MICROSTRUCTURE Fatigue life
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Surface Integrity Analysis of TC4 Machined by Combined Machining of Electric Arc-Mechanical Milling
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作者 XIN Shaokun LI Xuezhi +2 位作者 ZHOU Jianping ZHANG Zhaoming ZUO Hang 《新疆大学学报(自然科学版中英文)》 2025年第2期225-237,共13页
To address problems in surface integrity and machining allowance distribution during combined electric arc-mechanical milling,this paper takes TC4 as the research object,examines the influence of electric arc milling(... To address problems in surface integrity and machining allowance distribution during combined electric arc-mechanical milling,this paper takes TC4 as the research object,examines the influence of electric arc milling(EAM)depth on recast layer thickness and surface roughness,alongside an analysis of the recast layer’s organization characteristics and sur-face morphology.A comparative evaluation of cutting forces,surface roughness,and surface hardening is conducted between combined milling and conventional mechanical milling.Key findings reveal that electric arc machining produces a recast layer with a hardness of 313.21 HV.As the EAM depth increases,the localized recast layer thickness and peak-to-valley(PV)differ-ences also rise.To ensure effective surface defect removal,the machining allowance for subsequent mechanical milling must exceed the combined thickness of the recast layer and the PV difference.Under identical parameters,combined milling yields higher surface roughness(0.584μm)and greater surface hardening(10.4%)compared to mechanical milling alone,alongside an 18.716 N increase in cutting force.Response surface methodology(RSM)analysis identifies feed per tooth as the most significant factor affecting surface roughness,followed by spindle speed,with milling depth having the least influence. 展开更多
关键词 combined electric arc-mechanical milling TC4 surface integrity machining allowances recast layer
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DD9合金涡轮叶片电火花制孔工艺研究 被引量:1
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作者 梁威 祝玉兰 +2 位作者 佟浩 李勇 曹培尧 《电加工与模具》 北大核心 2025年第4期13-16,21,共5页
随着航空发动机涡轮前温度的提高,涡轮叶片逐步采用DD9合金等能承受更高温度的第三代单晶高温合金铸造而成,同时在铸造成形后的叶片表面加工出气膜孔进行气膜冷却来降低其表面温度,气膜孔的加工质量对叶片的冷却效果和疲劳寿命具有重要... 随着航空发动机涡轮前温度的提高,涡轮叶片逐步采用DD9合金等能承受更高温度的第三代单晶高温合金铸造而成,同时在铸造成形后的叶片表面加工出气膜孔进行气膜冷却来降低其表面温度,气膜孔的加工质量对叶片的冷却效果和疲劳寿命具有重要影响。为探索针对DD9合金涡轮叶片的低重铸层电火花制孔工艺,分别基于晶体管脉冲电源和Tr-RC电源进行了工艺实验和检测分析。结果表明,基于晶体管脉冲电源加工DD9合金的最大重铸层厚度可优化至18μm,基于Tr-RC电源加工DD9合金的最大重铸层厚度可降低至7μm,同时加工效率明显提高,单孔加工时间从26 s缩短至15 s。此外,若一定程度牺牲加工效率指标,基于Tr-RC电源加工DD9合金的最大重铸层厚度可降低至5μm,此时单孔加工时间增大至90 s。 展开更多
关键词 单晶涡轮叶片 DD9合金 电火花加工 重铸层
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电解质等离子体抛光去除重铸层的机理研究
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作者 廖华林 张亚欧 +2 位作者 卢军成 高强 赵万生 《电加工与模具》 北大核心 2025年第5期73-80,共8页
电火花加工产生的重铸层影响零件的疲劳寿命和表面完整性,采用电解质等离子体抛光技术去除电火花加工GH3536高温合金的残余重铸层。首先,分析抛光电路的伏安特性曲线,依据电压变化规律确定电解质等离子体抛光的工作区间:接着,采用高速... 电火花加工产生的重铸层影响零件的疲劳寿命和表面完整性,采用电解质等离子体抛光技术去除电火花加工GH3536高温合金的残余重铸层。首先,分析抛光电路的伏安特性曲线,依据电压变化规律确定电解质等离子体抛光的工作区间:接着,采用高速摄像机观察放电过程,发现电解质等离子体抛光是由阳极工件表面气层的形成与动态维持、工件表面氧化层的形成、等离子体通道的形成与击穿放电等环节协同实现,表面材料去除的主要动力源自放电过程的冲击波和气泡空化作用;最后,通过液相放电过程分析和加工后表面EDS分析,发现抛光后表层Cu,Zn、O等元素消失,Cr、Fe、Ni等元素含量上升,验证了基于电解质等离子体抛光方法去除重铸层的可行性。 展开更多
关键词 电解质等离子体 抛光技术 冲击波 重铸层
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短电弧-电化学复合加工单次放电凹坑形成影响研究
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作者 王家豪 胡国玉 +2 位作者 周建平 张晟晟 付斐 《热加工工艺》 北大核心 2025年第10期30-36,共7页
放电加工中凹坑形成的大小影响加工表面形貌的一致性,加工表面的重铸层影响加工表面质量。基于短电弧-电化学复合加工(SEACM)方法,在两种SEACM常用电源形式下对钛合金TC4进行单次放电实验,研究了不同电参数和不同浓度NaCl溶液对凹坑直... 放电加工中凹坑形成的大小影响加工表面形貌的一致性,加工表面的重铸层影响加工表面质量。基于短电弧-电化学复合加工(SEACM)方法,在两种SEACM常用电源形式下对钛合金TC4进行单次放电实验,研究了不同电参数和不同浓度NaCl溶液对凹坑直径、深度、凸起高度的影响,并通过仿真模拟凹坑形成与电化学溶解。结果表明,脉冲电源形式下凹坑直径与深度随着电压与脉宽的增加而增加,凸起高度先增大后减小;直流电源下凹坑直径与凸起高度变化与脉冲电源下一致,但凹坑的深度随电压增大呈现先增大后减小趋势;当NaCl溶液浓度提升时,凹坑逐渐减小,凹坑外沿的重铸层去除率最高,在10%加工浓度下为19.9%。 展开更多
关键词 短电弧-电化学复合加工 钛合金TC4 单次放电 凹坑形成 重铸层
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航空发动机涡轮叶片气膜孔制备仿真与试验研究
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作者 肖强 曾献城 魏群 《航空制造技术》 北大核心 2025年第14期34-41,共8页
传统的气膜孔加工方式,例如钻削、电火花等在加工过程中会产生裂纹、重铸层、锥度等问题,无法满足对气膜孔的高精度和高质量要求,从而对叶片的寿命产生不同程度的影响。不同于传统的气膜孔加工方式,激光加工气膜孔是一种高精度的加工方... 传统的气膜孔加工方式,例如钻削、电火花等在加工过程中会产生裂纹、重铸层、锥度等问题,无法满足对气膜孔的高精度和高质量要求,从而对叶片的寿命产生不同程度的影响。不同于传统的气膜孔加工方式,激光加工气膜孔是一种高精度的加工方式。但是,当飞溅物无法从气膜孔完全排出时,会在孔壁形成重铸层,进而对气膜孔的质量产生影响。本文主要对飞秒激光加工气膜孔的过程进行了仿真研究,分析了气膜孔从开始烧蚀到结束的形貌变化、所产生的金属气体的温度及速度变化,然后通过改变不同的激光参数,得出重铸层厚度受到激光功率和脉冲宽度的影响,激光功率增大可以减小重铸层厚度,但是激光功率也不能无限增大,否则会造成过度烧蚀。而脉冲宽度与重铸层厚度并不呈现简单的线性关系。因此,为了减小重铸层厚度,提出了先加工出尺寸小于实际所需的气膜孔,然后再对其进行扩孔的加工方式。通过试验验证,扩孔加工可以显著提高气膜孔质量。 展开更多
关键词 飞秒激光 气膜孔 仿真 重铸层 扩孔
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涡轮盘榫槽电火花线切割加工及其表面抛光研究
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作者 李显政 赵司评 +2 位作者 杨晓冬 段晓明 索景宇 《电加工与模具》 北大核心 2025年第S1期19-22,共4页
单向走丝电火花线切割加工具有加工精度高、适合难加工材料和复杂形状加工等特点,非常适合于加工榫槽,但加工产生的重铸层会对榫槽的疲劳强度带来不利影响。电解等离子体抛光技术具有加工速度快、表面质量好及材料去除量小的优势,可用... 单向走丝电火花线切割加工具有加工精度高、适合难加工材料和复杂形状加工等特点,非常适合于加工榫槽,但加工产生的重铸层会对榫槽的疲劳强度带来不利影响。电解等离子体抛光技术具有加工速度快、表面质量好及材料去除量小的优势,可用于电火花线切割加工榫槽的后续加工,能在去除重铸层的同时,避免降低其轮廓精度。因此,研究了单向走丝电火花线切割加工榫槽的轮廓精度和重铸层,并在优选的电解等离子体抛光参数(抛光电压325 V,抛光温度65℃,抛光时间8 min)条件下实现对榫槽的抛光,抛光后的表面粗糙度为Ra0.070μm、材料去除量为1.495 g,并且榫槽表面凹圆和凸圆部位的重铸层都得到有效去除。 展开更多
关键词 榫槽 涡轮盘 单向走丝电火花线切割加工 电解等离子体抛光 重铸层
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基于AMPK/ULK1/Beclin1通路研究补肾安胎合剂对复发性流产小鼠母胎界面血管重铸的影响
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作者 韩欣雨 程红 +1 位作者 余欣慧 韩萌 《中国中医药信息杂志》 2025年第12期113-119,共7页
目的 基于AMPK/ULK1/Beclin1通路观察补肾安胎合剂对复发性流产(RSA)小鼠母胎界面血管重铸的影响。方法 将CBA/J雌鼠与BALB/c雄鼠按2∶1比例合笼饲养建立正常妊娠小鼠(正常组),CBA/J雌鼠与DBA/2雄鼠合笼饲养建立RSA小鼠模型,将RSA小鼠... 目的 基于AMPK/ULK1/Beclin1通路观察补肾安胎合剂对复发性流产(RSA)小鼠母胎界面血管重铸的影响。方法 将CBA/J雌鼠与BALB/c雄鼠按2∶1比例合笼饲养建立正常妊娠小鼠(正常组),CBA/J雌鼠与DBA/2雄鼠合笼饲养建立RSA小鼠模型,将RSA小鼠随机分为模型组、自噬激动剂组和中药高、中、低剂量组,每组10只。自妊娠第1日起,中药高、中、低剂量组分别予补肾安胎合剂35.1、11.7、3.9 g/kg灌胃,正常组、模型组及自噬激动剂组灌胃等量生理盐水,连续15 d。观察胚胎发育情况,计算胚胎丢失率;HE染色观察蜕膜组织形态,ELISA检测蜕膜组织血管内皮生长因子(VEGF)、血管内皮钙黏蛋白(VE-cadherin)含量,Western blot检测蜕膜组织自噬相关蛋白腺苷酸活化蛋白激酶(AMPK)、UNC-51样激酶1(ULK1)、Beclin1、LC3B表达。结果 与正常组比较,模型组小鼠胚胎丢失率明显上升(P<0.01),蜕膜组织细胞排列无序,间质水肿,细胞核消失,有大量炎性细胞浸润,血管数量减少,蜕膜组织VEGF、VE-cadherin含量明显降低(P<0.01),AMPK、ULK1、Beclin1、LC3BⅡ/LC3BⅠ蛋白表达明显下降(P<0.01);与模型组比较,中药各剂量组和自噬激动剂组小鼠胚胎丢失率明显降低(P<0.05),蜕膜组织细胞排列整齐,间质水肿减轻,细胞核增多,炎性细胞浸润减少,血管数量增多,中药高、中剂量组和自噬激动剂组蜕膜组织VEGF、VE-cadherin含量明显升高(P<0.05),AMPK、ULK1、Beclin1、LC3BⅡ/LC3BⅠ蛋白表达明显升高(P<0.01)。结论 补肾安胎合剂可能通过激活AMPK/ULK1/Beclin1通路促进母胎界面血管重铸,提高RSA小鼠妊娠存活率,发挥治疗RSA作用。 展开更多
关键词 补肾安胎合剂 复发性流产 AMPK/ULK1/Beclin1通路 细胞自噬 血管重铸 小鼠
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