[目的]本研究旨在探究树莓果肉多糖RPP-1的体外免疫活性,并结合转录组学初步分析RPP-1免疫调节的作用机制。[方法]以来源于树莓果肉的多糖组分RPP-1为试验材料,分别采用CCK-8法和荧光探针法测定RPP-1对小鼠巨噬细胞RAW264.7细胞活力和...[目的]本研究旨在探究树莓果肉多糖RPP-1的体外免疫活性,并结合转录组学初步分析RPP-1免疫调节的作用机制。[方法]以来源于树莓果肉的多糖组分RPP-1为试验材料,分别采用CCK-8法和荧光探针法测定RPP-1对小鼠巨噬细胞RAW264.7细胞活力和吞噬能力的影响,利用RT-qPCR和ELISA分别从转录和蛋白质水平检测RPP-1对RAW264.7细胞上清液中NO和细胞因子的作用。同时,采用转录组学对差异表达基因(differentially expressed genes,DEG)进行基因本体(gene ontology,GO)功能注释、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析和蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络分析。[结果]RPP-1能显著促进小鼠巨噬细胞RAW264.7的活力和吞噬能力,并明显增强细胞培养上清液中的NO、IL-6、IL-1β和TNF-α水平,提高iNOS、TNF-α、IL-6和IL-1βmRNA的相对表达水平,显示出较强的体外免疫增强作用。转录组学分析发现,与空白组相比,经RPP-1处理的RAW264.7细胞中共有286个与免疫调节相关的DEG,其中111个DEG下调,175个DEG上调;GO功能注释结果表明这些DEG主要参与细胞过程、生物调节、代谢过程、发育过程和免疫系统过程;KEGG富集分析结果显示差异基因显著富集于系统性红斑狼疮、细胞因子-细胞因子受体相互作用和TNF信号通路;PPI网络分析发现枢纽基因为IL-6、IL-1β、Rad51ap1、H2afx、Birc5、Ccl5和Pbk。[结论]树莓果肉多糖RPP-1对小鼠巨噬细胞RAW264.7具有免疫增强作用,结合体外活性试验与转录组学分析结果,RPP-1的免疫增强作用可能通过细胞因子-细胞因子受体相互作用及TNF信号传导途径调节实现,且其关键基因为IL-6、IL-1β。展开更多
Europe is grappling with a colossal textile waste problem.Over 125 million tonnes of raw materials are devoured by the global industry each year,yet a mere fraction-less than 1%-of these fibres originate from recycled...Europe is grappling with a colossal textile waste problem.Over 125 million tonnes of raw materials are devoured by the global industry each year,yet a mere fraction-less than 1%-of these fibres originate from recycled textiles.The majority faces an unsustainable fate in landfills,incinerators,or is exported.A pivotal new report by Systemiq,"The Textile Recycling Breakthrough,"offers both a stark assessment and a strategic roadmap:Europe has the potential to amplify polyester textile recycling nearly tenfold by 2035,but this hinges on immediate,decisive action from policymakers and the industry.展开更多
文摘[目的]本研究旨在探究树莓果肉多糖RPP-1的体外免疫活性,并结合转录组学初步分析RPP-1免疫调节的作用机制。[方法]以来源于树莓果肉的多糖组分RPP-1为试验材料,分别采用CCK-8法和荧光探针法测定RPP-1对小鼠巨噬细胞RAW264.7细胞活力和吞噬能力的影响,利用RT-qPCR和ELISA分别从转录和蛋白质水平检测RPP-1对RAW264.7细胞上清液中NO和细胞因子的作用。同时,采用转录组学对差异表达基因(differentially expressed genes,DEG)进行基因本体(gene ontology,GO)功能注释、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析和蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络分析。[结果]RPP-1能显著促进小鼠巨噬细胞RAW264.7的活力和吞噬能力,并明显增强细胞培养上清液中的NO、IL-6、IL-1β和TNF-α水平,提高iNOS、TNF-α、IL-6和IL-1βmRNA的相对表达水平,显示出较强的体外免疫增强作用。转录组学分析发现,与空白组相比,经RPP-1处理的RAW264.7细胞中共有286个与免疫调节相关的DEG,其中111个DEG下调,175个DEG上调;GO功能注释结果表明这些DEG主要参与细胞过程、生物调节、代谢过程、发育过程和免疫系统过程;KEGG富集分析结果显示差异基因显著富集于系统性红斑狼疮、细胞因子-细胞因子受体相互作用和TNF信号通路;PPI网络分析发现枢纽基因为IL-6、IL-1β、Rad51ap1、H2afx、Birc5、Ccl5和Pbk。[结论]树莓果肉多糖RPP-1对小鼠巨噬细胞RAW264.7具有免疫增强作用,结合体外活性试验与转录组学分析结果,RPP-1的免疫增强作用可能通过细胞因子-细胞因子受体相互作用及TNF信号传导途径调节实现,且其关键基因为IL-6、IL-1β。
文摘Europe is grappling with a colossal textile waste problem.Over 125 million tonnes of raw materials are devoured by the global industry each year,yet a mere fraction-less than 1%-of these fibres originate from recycled textiles.The majority faces an unsustainable fate in landfills,incinerators,or is exported.A pivotal new report by Systemiq,"The Textile Recycling Breakthrough,"offers both a stark assessment and a strategic roadmap:Europe has the potential to amplify polyester textile recycling nearly tenfold by 2035,but this hinges on immediate,decisive action from policymakers and the industry.
