Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been lin...Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb^(2+) uptake and the investigation of the plant’s physiological responses to Pb^(2+) pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb^(2+) accumulation after exogenous Pb^(2+) treatment. A significant variation in Pb^(2+) content was observed among the germplasms, indicating rich genetic diversity. Using RAPD markers, seven loci were identified to be significantly associated with Pb^(2+) uptake, with locus 43 (R^(2) = 6.72%) and locus 53 (R^(2) = 5.39%) showing the strongest correlations. Marker validation was performed using five low- and five high-accumulating accessions. Two representative germplasms were further subjected to 0, 500 and 1000 mg/kg Pb^(2+) treatments for 40 days. Pb^(2+) content, membrane lipid peroxidation, and redox enzyme activities (SOD, POD and CAT) were measured across different organs. Organs with greater soil contact (roots) exhibited higher Pb^(2+) accumulation and oxidative damage. POD and CAT activities were markedly induced by Pb^(2+) stress, while SOD response was limited. This study provides a theoretical foundation for breeding low Pb^(2+)-accumulating H. cordata varieties through marker-assisted selection (MAS) and supports their safe use and application in phytoremediation.展开更多
Genetic relationships between Ziziphus jujuba and Ziziphus spinosa populations were studied using RAPD data in population genetics. 275 loci were gained among which 249 loci were polymorphic by 22 primers. The polymor...Genetic relationships between Ziziphus jujuba and Ziziphus spinosa populations were studied using RAPD data in population genetics. 275 loci were gained among which 249 loci were polymorphic by 22 primers. The polymorphic loci percentage was 89% among Z.spinosa population while that of Z.jujuba population was 56%. 31 specific RAPD markers were detected on 3 Z.jujuba varieties and 15 Z.spinosa forms. Cluster analysis showed: when λ=12, the forms tested were divided into 2 groups. One included Z. jujuba cv. Zanhuangdazao, Yudichuangan, Yuanshichuangan, Xiaochuangan, Qiantai No.1, Luzhuangchuangan; the other included Z. jujuba cv. Zilingdan and 26 Z. spinosa forms. All above indicated that Z. jujuba and Z.spinosa should be regarded as two separate species.展开更多
基金supported by the Guizhou Provincial Department of Agriculture and Rural Afairs,the Guizhou Provincial Department of Science and Technology,and the Guizhou Provincial Department of Education.Funding Project are Guizhou Highland Specialty Vegetable Green Production Science,Technology Innovation Talent Team(Qiankehe Platform Talent-CXTD[2022]003)Guizhou Mountain Agriculture Key Core Technology Research Project(GZNYGJHX 2023013)Platform construction project of Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province(Qian Jiao Ji[2022]No.040).
文摘Houttuynia cordata, a characteristic edible and medicinal plant in southwestern China, is prone to absorbing lead (Pb^(2+)). Excessive consumption may lead to Pb^(2+) accumulation in the human body, which has been linked to serious health risks such as neurotoxicity, kidney damage, anemia, and developmental disorders, particularly in children. Therefore, the development of molecular markers associated with Pb^(2+) uptake and the investigation of the plant’s physiological responses to Pb^(2+) pollution are of great significance. In this study, 72 H. cordata germplasms were evaluated for Pb^(2+) accumulation after exogenous Pb^(2+) treatment. A significant variation in Pb^(2+) content was observed among the germplasms, indicating rich genetic diversity. Using RAPD markers, seven loci were identified to be significantly associated with Pb^(2+) uptake, with locus 43 (R^(2) = 6.72%) and locus 53 (R^(2) = 5.39%) showing the strongest correlations. Marker validation was performed using five low- and five high-accumulating accessions. Two representative germplasms were further subjected to 0, 500 and 1000 mg/kg Pb^(2+) treatments for 40 days. Pb^(2+) content, membrane lipid peroxidation, and redox enzyme activities (SOD, POD and CAT) were measured across different organs. Organs with greater soil contact (roots) exhibited higher Pb^(2+) accumulation and oxidative damage. POD and CAT activities were markedly induced by Pb^(2+) stress, while SOD response was limited. This study provides a theoretical foundation for breeding low Pb^(2+)-accumulating H. cordata varieties through marker-assisted selection (MAS) and supports their safe use and application in phytoremediation.
文摘Genetic relationships between Ziziphus jujuba and Ziziphus spinosa populations were studied using RAPD data in population genetics. 275 loci were gained among which 249 loci were polymorphic by 22 primers. The polymorphic loci percentage was 89% among Z.spinosa population while that of Z.jujuba population was 56%. 31 specific RAPD markers were detected on 3 Z.jujuba varieties and 15 Z.spinosa forms. Cluster analysis showed: when λ=12, the forms tested were divided into 2 groups. One included Z. jujuba cv. Zanhuangdazao, Yudichuangan, Yuanshichuangan, Xiaochuangan, Qiantai No.1, Luzhuangchuangan; the other included Z. jujuba cv. Zilingdan and 26 Z. spinosa forms. All above indicated that Z. jujuba and Z.spinosa should be regarded as two separate species.
