摘要
目的 利用RAPD技术探讨厚朴种内关系、道地性问题 ;寻找正品厚朴DNA指纹特征。方法 选择代表厚朴主要分布区的 11个产地 33个的个体材料作为样本 ,经DNA提取 ,用 74个随机引物进行PCR扩增。结果 从17个引物中得到 116条带 ,用于种内关系探讨 ,经聚类分析 ,得到 3个主要分支及反映正品种及优良品种的特异性引物和条带。结论 (1)将厚朴分为 3个地理宗更合适 ,分别是 :典型的厚朴、典型的凹叶厚朴及中间类型 ,这与叶的形态等性状一致 ;(2 )“川朴”及“温朴”有明显的遗传分化 ,且与有效成分相关 ,故厚朴的道地性主要来源于遗传差异 ;(3)
AIM To discuss the intraspecific relationship in Magnolia officinalis and the genuineness of Cortex Magnoliae officinalis , and to find some DNA characters of certified “Houpo”. METHODS Thirty three samples from eleven locations, which can represent most of the distribution of M.officinalis , were selected. The total DNA was extracted. Severty four random primers were tried to get good amplification. RESULTS One hundred and sixteen bands amplified from seventeen primers, were clustered by NTSYS pc software. Three branches were obtained. Some distinctive primers and bands, which represent certified species or fine breed, were obtained also. CONCLUSION 1) M.officinalis should be divided into three geographic clans instead of two subspecies or varieties, they are, a) typical officinalis , b) typical biloba and c) Middle type. This conclusion agrees with the leaf form and other characters. 2) The genetic difference between “Chuanpo” and “Wenpo” is evident and the difference is in correspondence with the quantities of their chemical constituents. So, the genetic difference is the main reason of the genuineness of Cortex Magnoliae officinalis . 3) These results may be used to establish DNA database for identification of Cortex Magnoliae officinalis .
出处
《药学学报》
CAS
CSCD
北大核心
2001年第5期386-389,共4页
Acta Pharmaceutica Sinica
