Hepatocellular carcinoma and intestinal damage are the most commonly reported issues associated with aflatoxin-B1(AFB1)intoxications.AFB1 is biotransformed by cytochrome P4501A2(CYP1A2)and 3A4(CYP3A4)to create a more ...Hepatocellular carcinoma and intestinal damage are the most commonly reported issues associated with aflatoxin-B1(AFB1)intoxications.AFB1 is biotransformed by cytochrome P4501A2(CYP1A2)and 3A4(CYP3A4)to create a more toxic form,known as AFB1-8,9-epoxide.This study is aimed to investigate the protective effects of catechin and quercitrin,naturally occurring compounds found in neem leaves(Azadirachta indica),against AFB1-induced cytotoxicity in human liver(HepG2)and colon(Caco-2)cell lines.Both compounds significantly improved cell viability after exposure to AFB1 in a dose-dependent manner.In-silico docking simulations revealed promising interactions of these compounds against CYP1A2 and CYP3A4,suggesting potential mechanisms for the modulation of these enzymes’activity through competitive inhibition of AFB1 binding.This could lead to a reduction in the formation of toxic metabolites.These findings emphasize the potential of catechin and quercitrin as dietary supplements to mitigate AFB1 toxicity.展开更多
[Objectives]This study was conducted to establish a quality control method for mulberry-parasitizing Taxillus chinensis by detecting both quercitrin,an inherent component of the medicinal material,and 1-deoxynojirimyc...[Objectives]This study was conducted to establish a quality control method for mulberry-parasitizing Taxillus chinensis by detecting both quercitrin,an inherent component of the medicinal material,and 1-deoxynojirimycin(1-DNJ),a characteristic component derived from the mulberry host,using a"dual-substance detection"approach.[Methods]Ten batches of mulberry-parasitizing T.chinensis samples from different producing areas were collected,and T.chinensis samples parasitizing oil-tea camellia and oleander were used as non-mulberry host controls.TLC was used for qualitative identification of quercitrin and 1-DNJ in the samples.HPLC-UV was employed for quantitative determination of quercitrin,and HPLC-ELSD was used for quantitative determination of 1-DNJ.[Results]Under UV light at 365 nm,distinct quercitrin spots(Rf=0.46)were observed in all ten batches of mulberry-parasitizing Taxillus chinensis samples and in the two control samples(Taxillus chinensis parasitizing oil-tea camellia and oleander).In contrast,no corresponding quercitrin spots were detected in the host samples(mulberry branches,oil-tea camellia branches,and oleander branches).Under natural light,distinct 1-DNJ spots(Rf=0.324)were observed in all ten batches of mulberry-parasitizing T.chinensis samples and in their host mulberry branches.In contrast,no corresponding 1-DNJ spots were detected in T.chinensis samples parasitizing oil-tea camellia or oleander,nor in their host branches(oil-tea camellia branches and oleander branches).The average recovery of quercitrin was 99.5%(RSD=2.95%).The average quercitrin content in the ten batches of mulberry-parasitizing T.chinensis samples ranged from 1.98 to 3.11 mg/g,while no quercitrin was detected in the host mulberry branch samples.The average recovery of 1-DNJ was 98.03%(RSD=1.15%).The 1-DNJ content in the ten batches of mulberry-parasitizing T.chinensis samples ranged from 1.35 to 5.08 mg/g,while that in the host mulberry branches ranged from 3.21 to 9.41 mg/g.No 1-DNJ was detected in T.chinensis samples parasitizing oil-tea camellia or oleander,nor in their host branches(oil-tea camellia branches and oleander branches).[Conclusions]The detection method based on the"dual-component"approach using quercitrin and 1-DNJ is highly specific and simple to operate.It enables both the identification of the host origin and the quality control of mulberry-parasitizing T.chinensis.This"dual-component"quality control method has significant implications for guiding the quality control of parasitic herbs derived from other host plants.展开更多
Neuroinflammation is an inflammatory response in the central nervous system associated with various neurological conditions.The inflammatory process is typically treated with non-steroidal and steroidal anti-inflammat...Neuroinflammation is an inflammatory response in the central nervous system associated with various neurological conditions.The inflammatory process is typically treated with non-steroidal and steroidal anti-inflammatory drugs,which have a range of serious adverse effects.As an alternative,naturally derived molecules such as quercetin and its derivatives show promising anti-inflammatory properties and beneficial effects on various physiological functions.Our objective was to synthesize the evidence on the anti-inflammatory effect of quercetin and its derivatives in in vivo models,in the face of neuroinflammatory insults induced by lipopolysaccharide,through a systematic review and meta-analysis.A search of the preclinical literature was conducted across four databases(Pub Med,Web of Science,Scielo,and Google Scholar).Studies were selected based on inclusion and exclusion criteria,assessed for methodological quality using CAMARADES,and risk of bias using the SYRCLE tool,and data were extracted from the studies.The quantitative assessment of quercetin effects on the expression of pro-inflammatory cytokines and microgliosis was performed through a meta-analysis.A total of 384 potentially relevant articles were identified,of which 11 studies were included in the analysis.The methodological quality was assessed,resulting in an average score of 5.8/10,and the overall risk of bias analysis revealed a lack of methodological clarity in most studies.Furthermore,through the meta-analysis,it was observed that treatment with quercetin statistically reduces pro-inflammatory cytokines,such as tumor necrosis factor alpha,interleukin 6,interleukin 1β(n=89;SMD=–2.00;95%CI:–3.29 to–0.71),and microgliosis(n=33;SMD=–2.56;95%CI:–4.07 to–1.10).In terms of underlying mechanisms,quercetin and its derivatives exhibit antioxidant and anti-apoptotic properties,possibly through the nuclear factor erythroid 2-related factor 2(Nrf2)/HO-1 pathways,increasing the expression of antioxidant enzymes and reducing reactive species,and modulating the caspase pathway,increasing levels of anti-apoptotic proteins and decreasing proapoptotic proteins.Quercetin and its derivatives exhibit highly pleiotropic actions that simultaneously contribute to preventing neuroinflammation.However,despite promising results in animal models,future directions should focus on well-designed clinical studies to assess the safety,bioavailability,and efficacy of quercetin and its derivatives in humans.Additionally,standardization of methods and dosages in studies is crucial to ensure consistency of findings and optimize their application in clinical settings.展开更多
Objective：The study was designed to investigate the molecular mechanism of quercitrin on osteogenic differentiation and adipogenic differentiation of r BMSCs.Methods：r BMSCs were harvested from SD rats,and determina...Objective：The study was designed to investigate the molecular mechanism of quercitrin on osteogenic differentiation and adipogenic differentiation of r BMSCs.Methods：r BMSCs were harvested from SD rats,and determination of alkaline phosphatase（ALP）activity,quantification of mineralization by Alizarin Red S staining,and the m RNA expression of osteogenic differentiation markers（Runx2,BMP-2,and OSX）by RT-PCR after r BMSCs stimulated by osteogenic induction with（0.1–10）μg/m L of quercitrin,quantification of Lipid droplet by Oil Red O staining and the m RNA expression of adipogenic differentiation marker（,and a P2）by RT-PCR after r BMSCs stimulated by adipogenic induction with（0.1-10）μg/m L of quercitrin.Results：Quercitrin can up-regulate the m RNA expression of osteogenic differentiation markers（Runx2,BMP-2,and OSX）and increase ALP activity and mineralization after osteogenic induction,on the other hand quercitrin can suppress the m RNA expression of adipogenic differentiation markers（,and a P2）and decrease lipid droplet after adipogenic induction.Conclusion：This study suggested that quercitrin not only stimulated osteogenic differentiation but also inhibited adipogenic differentiation of r BMSCs,which was associated with the up-regulation of Runx2,BMP-2,and OSX m RNA expression and the down-regulation of,and a P2 m RNA expression.展开更多
[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol ...[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol in trichosanthes peel samples collected from different sites were determined by high-performance liquid chromatography.With each component as an index,principal component analysis and cluster analysis were performed.[Results]The contents of flavonoids in the trichosanthes peel samples from different sites were different.The cumulative variance contribution rate of isoquercitrin was 95.514%.The results of cluster analysis show that the quality of trichosanthes peel from Anhui was the best.[Conclusions]Taking isoquercitrin as a common factor to carry out content determination will help to better control the quality of trichosanthes peel.展开更多
基金supported by funding from the iEGG and Animal Biotechnology Research Center from The Feature Area Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education(MOE)in Taiwan.
文摘Hepatocellular carcinoma and intestinal damage are the most commonly reported issues associated with aflatoxin-B1(AFB1)intoxications.AFB1 is biotransformed by cytochrome P4501A2(CYP1A2)and 3A4(CYP3A4)to create a more toxic form,known as AFB1-8,9-epoxide.This study is aimed to investigate the protective effects of catechin and quercitrin,naturally occurring compounds found in neem leaves(Azadirachta indica),against AFB1-induced cytotoxicity in human liver(HepG2)and colon(Caco-2)cell lines.Both compounds significantly improved cell viability after exposure to AFB1 in a dose-dependent manner.In-silico docking simulations revealed promising interactions of these compounds against CYP1A2 and CYP3A4,suggesting potential mechanisms for the modulation of these enzymes’activity through competitive inhibition of AFB1 binding.This could lead to a reduction in the formation of toxic metabolites.These findings emphasize the potential of catechin and quercitrin as dietary supplements to mitigate AFB1 toxicity.
基金Supported by Youth Science Fund Project Guangxi Natural Science Foundation(2023GXNSFBA026280)Univeristy-level Scientific Research Project of Faculty of Chinese Medicine Science of Guangxi University of Chinese Medicine(2025MS010)Key Project of Guangxi University of Chinese Medicine Joint Fund Project(Special Project of Qinzhou Hospital of Traditional Chinese Medicine)(2024QZ001).
文摘[Objectives]This study was conducted to establish a quality control method for mulberry-parasitizing Taxillus chinensis by detecting both quercitrin,an inherent component of the medicinal material,and 1-deoxynojirimycin(1-DNJ),a characteristic component derived from the mulberry host,using a"dual-substance detection"approach.[Methods]Ten batches of mulberry-parasitizing T.chinensis samples from different producing areas were collected,and T.chinensis samples parasitizing oil-tea camellia and oleander were used as non-mulberry host controls.TLC was used for qualitative identification of quercitrin and 1-DNJ in the samples.HPLC-UV was employed for quantitative determination of quercitrin,and HPLC-ELSD was used for quantitative determination of 1-DNJ.[Results]Under UV light at 365 nm,distinct quercitrin spots(Rf=0.46)were observed in all ten batches of mulberry-parasitizing Taxillus chinensis samples and in the two control samples(Taxillus chinensis parasitizing oil-tea camellia and oleander).In contrast,no corresponding quercitrin spots were detected in the host samples(mulberry branches,oil-tea camellia branches,and oleander branches).Under natural light,distinct 1-DNJ spots(Rf=0.324)were observed in all ten batches of mulberry-parasitizing T.chinensis samples and in their host mulberry branches.In contrast,no corresponding 1-DNJ spots were detected in T.chinensis samples parasitizing oil-tea camellia or oleander,nor in their host branches(oil-tea camellia branches and oleander branches).The average recovery of quercitrin was 99.5%(RSD=2.95%).The average quercitrin content in the ten batches of mulberry-parasitizing T.chinensis samples ranged from 1.98 to 3.11 mg/g,while no quercitrin was detected in the host mulberry branch samples.The average recovery of 1-DNJ was 98.03%(RSD=1.15%).The 1-DNJ content in the ten batches of mulberry-parasitizing T.chinensis samples ranged from 1.35 to 5.08 mg/g,while that in the host mulberry branches ranged from 3.21 to 9.41 mg/g.No 1-DNJ was detected in T.chinensis samples parasitizing oil-tea camellia or oleander,nor in their host branches(oil-tea camellia branches and oleander branches).[Conclusions]The detection method based on the"dual-component"approach using quercitrin and 1-DNJ is highly specific and simple to operate.It enables both the identification of the host origin and the quality control of mulberry-parasitizing T.chinensis.This"dual-component"quality control method has significant implications for guiding the quality control of parasitic herbs derived from other host plants.
基金supported by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil(CAPES)[Finance Code 001](to MGS)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq)fellowship[research grants 309840/2022-8]。
文摘Neuroinflammation is an inflammatory response in the central nervous system associated with various neurological conditions.The inflammatory process is typically treated with non-steroidal and steroidal anti-inflammatory drugs,which have a range of serious adverse effects.As an alternative,naturally derived molecules such as quercetin and its derivatives show promising anti-inflammatory properties and beneficial effects on various physiological functions.Our objective was to synthesize the evidence on the anti-inflammatory effect of quercetin and its derivatives in in vivo models,in the face of neuroinflammatory insults induced by lipopolysaccharide,through a systematic review and meta-analysis.A search of the preclinical literature was conducted across four databases(Pub Med,Web of Science,Scielo,and Google Scholar).Studies were selected based on inclusion and exclusion criteria,assessed for methodological quality using CAMARADES,and risk of bias using the SYRCLE tool,and data were extracted from the studies.The quantitative assessment of quercetin effects on the expression of pro-inflammatory cytokines and microgliosis was performed through a meta-analysis.A total of 384 potentially relevant articles were identified,of which 11 studies were included in the analysis.The methodological quality was assessed,resulting in an average score of 5.8/10,and the overall risk of bias analysis revealed a lack of methodological clarity in most studies.Furthermore,through the meta-analysis,it was observed that treatment with quercetin statistically reduces pro-inflammatory cytokines,such as tumor necrosis factor alpha,interleukin 6,interleukin 1β(n=89;SMD=–2.00;95%CI:–3.29 to–0.71),and microgliosis(n=33;SMD=–2.56;95%CI:–4.07 to–1.10).In terms of underlying mechanisms,quercetin and its derivatives exhibit antioxidant and anti-apoptotic properties,possibly through the nuclear factor erythroid 2-related factor 2(Nrf2)/HO-1 pathways,increasing the expression of antioxidant enzymes and reducing reactive species,and modulating the caspase pathway,increasing levels of anti-apoptotic proteins and decreasing proapoptotic proteins.Quercetin and its derivatives exhibit highly pleiotropic actions that simultaneously contribute to preventing neuroinflammation.However,despite promising results in animal models,future directions should focus on well-designed clinical studies to assess the safety,bioavailability,and efficacy of quercetin and its derivatives in humans.Additionally,standardization of methods and dosages in studies is crucial to ensure consistency of findings and optimize their application in clinical settings.
基金supported by National Natural Science Foundation of China(No.81160508)The Science and Technology Landing Program Project of Colleges and University in Jiangxi Province(KJLD14058)
文摘Objective：The study was designed to investigate the molecular mechanism of quercitrin on osteogenic differentiation and adipogenic differentiation of r BMSCs.Methods：r BMSCs were harvested from SD rats,and determination of alkaline phosphatase（ALP）activity,quantification of mineralization by Alizarin Red S staining,and the m RNA expression of osteogenic differentiation markers（Runx2,BMP-2,and OSX）by RT-PCR after r BMSCs stimulated by osteogenic induction with（0.1–10）μg/m L of quercitrin,quantification of Lipid droplet by Oil Red O staining and the m RNA expression of adipogenic differentiation marker（,and a P2）by RT-PCR after r BMSCs stimulated by adipogenic induction with（0.1-10）μg/m L of quercitrin.Results：Quercitrin can up-regulate the m RNA expression of osteogenic differentiation markers（Runx2,BMP-2,and OSX）and increase ALP activity and mineralization after osteogenic induction,on the other hand quercitrin can suppress the m RNA expression of adipogenic differentiation markers（,and a P2）and decrease lipid droplet after adipogenic induction.Conclusion：This study suggested that quercitrin not only stimulated osteogenic differentiation but also inhibited adipogenic differentiation of r BMSCs,which was associated with the up-regulation of Runx2,BMP-2,and OSX m RNA expression and the down-regulation of,and a P2 m RNA expression.
基金College Students'Innovation and Entrepreneurship Training Program of Liaoning Province(201911430039).
文摘[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol in trichosanthes peel samples collected from different sites were determined by high-performance liquid chromatography.With each component as an index,principal component analysis and cluster analysis were performed.[Results]The contents of flavonoids in the trichosanthes peel samples from different sites were different.The cumulative variance contribution rate of isoquercitrin was 95.514%.The results of cluster analysis show that the quality of trichosanthes peel from Anhui was the best.[Conclusions]Taking isoquercitrin as a common factor to carry out content determination will help to better control the quality of trichosanthes peel.
