摘要
Hepatocellular carcinoma and intestinal damage are the most commonly reported issues associated with aflatoxin-B1(AFB1)intoxications.AFB1 is biotransformed by cytochrome P4501A2(CYP1A2)and 3A4(CYP3A4)to create a more toxic form,known as AFB1-8,9-epoxide.This study is aimed to investigate the protective effects of catechin and quercitrin,naturally occurring compounds found in neem leaves(Azadirachta indica),against AFB1-induced cytotoxicity in human liver(HepG2)and colon(Caco-2)cell lines.Both compounds significantly improved cell viability after exposure to AFB1 in a dose-dependent manner.In-silico docking simulations revealed promising interactions of these compounds against CYP1A2 and CYP3A4,suggesting potential mechanisms for the modulation of these enzymes’activity through competitive inhibition of AFB1 binding.This could lead to a reduction in the formation of toxic metabolites.These findings emphasize the potential of catechin and quercitrin as dietary supplements to mitigate AFB1 toxicity.
基金
supported by funding from the iEGG and Animal Biotechnology Research Center from The Feature Area Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education(MOE)in Taiwan.
