Pyrrosia petiolosa,Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium(PF)and commonly used as Chinese herbal medicines.Due to the similar morphological features of PF and its adu...Pyrrosia petiolosa,Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium(PF)and commonly used as Chinese herbal medicines.Due to the similar morphological features of PF and its adulterants,common DNA barcodes cannot accurately distinguish PF species.Knowledge of the chloroplast(cp)genome is widely used in species identification,molecular marker and phylogenetic analyses.Herein,we determined the complete cp genomes of three original species of PF via highthroughput sequencing technologies.The three cp genomes exhibited a typical quadripartite structure with sizes ranging from 158165 to 163026 bp.The cp genomes of P.petiolosa and P.lingua encoded 130 genes,whilst that of P.sheareri encoded 131 genes.The complete cp genomes were compared,and five highly divergent regions of pet A-psb J,mat K-rps16,ndh C-trn M,psb M-pet N and psa Cndh E were screened as potential DNA barcodes for identification of Pyrrosia genus species.The phylogenetic tree we obtained indicated that P.petiolosa and P.lingua are clustered in a single clade and,thus,share a close relationship.This study provides invaluable information for further studies on the species identification,taxonomy and phylogeny of Pyrrosia genus species.展开更多
An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography （UFLC） method for quality evaluations ofPyrrosia petiolosa （Christ） Ching from 20 regions of China was developed in this stud...An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography （UFLC） method for quality evaluations ofPyrrosia petiolosa （Christ） Ching from 20 regions of China was developed in this study. Ten marker compounds were simultaneously quantified, including 5-caffeoylquinic acid （5-CQA）, 3-caffeoylquinic acid （3-CQA）, 4-caffeoylquinic acid （4-CQA）, 1-caffeoylquinic acid （1-CQA）, 3,5-dicaffeoylquinic acid （3,5-diCQA）, 4,5-dicaffeoylquinic acid （4,5-diCQA）, 3,4-dicaffeoylquinic acid （3,4-diCQA）, astragalin, kaempferol-3,7-di-O-glucoside and （±）eriodictyol-7-O-β-D-glucuronide. Chromatography was performed on a Kromasil 100-2.5C18 （100 mm×2.1 mm, 2.5 μm） C18 column with gradient elution. The mobile phases consisted of 0.1% formic acid/water （A） and 0.1% formic acid/methanol （B）. The detection wavelength was set at 326 nm and the flow rate was 0.4 mL/min. Ten components were separated well with good linearity （r2〉0.9998）, precision, repeatability, stability. The recovery was in the range of 99.08%-102.77%. The results showed that the content determination using RP-UFLC-DAD fingerprint technique provides an efficient, sensitive, accurate and rapid analytical method for quality assessment ofP. petiolosa （Christ） Ching. Cluster analysis and principal components analysis were successfully applied to analyze 20 samples, the results revealed that the method was efficient and authentic to distinguish producing areas and the source of P. petiolosa （Christ） Ching. Keywords： Pyrrosiapetiolosa （Christ） Ching, Caffeoylquinic acids, Flavonoids, Multicomponent determination, UFLC展开更多
A new flavone diglycoside, named as pyrropetioside A 1 was isolated from Pyrrosia petiolosa. Its structrue was elucidated as 7-O-[6-O-(α-L-arabifuranosyl)-β-D-glucopyranosyl]-gossypetin by means of chemical and spec...A new flavone diglycoside, named as pyrropetioside A 1 was isolated from Pyrrosia petiolosa. Its structrue was elucidated as 7-O-[6-O-(α-L-arabifuranosyl)-β-D-glucopyranosyl]-gossypetin by means of chemical and spectroscopie methods including IR, MS, 1 D and 2D NMR techniques.展开更多
[Objectives]The therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed based on the biolabel pattern.[Methods]The chemical components of Folium Pyrrosiae were analyzed by liquid chromatography-ma...[Objectives]The therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed based on the biolabel pattern.[Methods]The chemical components of Folium Pyrrosiae were analyzed by liquid chromatography-mass spectrometry(LC-MS).Ten databases,including Pubchem,CTD,BindingDB,HERB,TCMIP,ETCM,SwissTargetPrediction,SuperPred webserver,TargetNet and SEA,were used in turn to retrieve the targets of related components,and key components were obtained according to the enrichment degree of targets.The obtained targets were imported into the STRING database to obtain PPI information and screen out core targets.The DAVID database was employed to analyze KEGG pathways of core targets and obtain key pathways.A key component-core target-key pathway network of Folium Pyrrosiae was constructed by Cytoscape3.10.1 software.The obtained KEGG pathways were input into the CTD database to predict corresponding diseases,and discussion and analysis were carried out.[Results]Ten key components,30 potential targets and 10 key pathways were screened out,and they participated in many diseases,of which five diseases were mainly analyzed.[Conclusions]Folium Pyrrosiae had the characteristic of multi-component,multi-target and multi-pathway synergistic effect in the treatment of lung cancer,type 2 diabetes,atherosclerosis,liver cancer,prostate cancer and other diseases,and the therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed through the biolabel pattern.This study provides a research basis for further developing new functions of Folium Pyrrosiae.展开更多
有柄石韦(Pyrrosia petiolosa)是水龙骨科石韦属植物,也是一种常用的中药材,被广泛用于治疗肾盂肾炎、慢性支气管炎、尿路结石等病症。通过检索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的有柄石韦相关文献,对有柄...有柄石韦(Pyrrosia petiolosa)是水龙骨科石韦属植物,也是一种常用的中药材,被广泛用于治疗肾盂肾炎、慢性支气管炎、尿路结石等病症。通过检索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的有柄石韦相关文献,对有柄石韦近四十年的化学成分和生物活性研究进行系统地梳理、归纳,发现目前已从有柄石韦中分离出超过100种化合物,包括黄酮类、三萜类、木脂素类、酚类和其他类等。有柄石韦提取物具有显著的生物活性,包括抗氧化、抗菌、抗炎、利尿、促进伤口愈合、抗肾结石、抗糖尿病等。可为有柄石韦药用资源后续深入系统地研究和临床开发提供科学依据和理论参考。展开更多
基金supported by the Major Scientific and Technological Special Project for“Significant New Drugs Creation”(No.2018ZX09711001-008-007)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(CIFMS,No.2016-I2M-3-016)。
文摘Pyrrosia petiolosa,Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium(PF)and commonly used as Chinese herbal medicines.Due to the similar morphological features of PF and its adulterants,common DNA barcodes cannot accurately distinguish PF species.Knowledge of the chloroplast(cp)genome is widely used in species identification,molecular marker and phylogenetic analyses.Herein,we determined the complete cp genomes of three original species of PF via highthroughput sequencing technologies.The three cp genomes exhibited a typical quadripartite structure with sizes ranging from 158165 to 163026 bp.The cp genomes of P.petiolosa and P.lingua encoded 130 genes,whilst that of P.sheareri encoded 131 genes.The complete cp genomes were compared,and five highly divergent regions of pet A-psb J,mat K-rps16,ndh C-trn M,psb M-pet N and psa Cndh E were screened as potential DNA barcodes for identification of Pyrrosia genus species.The phylogenetic tree we obtained indicated that P.petiolosa and P.lingua are clustered in a single clade and,thus,share a close relationship.This study provides invaluable information for further studies on the species identification,taxonomy and phylogeny of Pyrrosia genus species.
基金Study of Safety Testing Techniques and Standards on New Traditional Chinese Drug(National Key Science and Technology Special Projects,Grant No.2014ZX09304307-001-001)
文摘An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography （UFLC） method for quality evaluations ofPyrrosia petiolosa （Christ） Ching from 20 regions of China was developed in this study. Ten marker compounds were simultaneously quantified, including 5-caffeoylquinic acid （5-CQA）, 3-caffeoylquinic acid （3-CQA）, 4-caffeoylquinic acid （4-CQA）, 1-caffeoylquinic acid （1-CQA）, 3,5-dicaffeoylquinic acid （3,5-diCQA）, 4,5-dicaffeoylquinic acid （4,5-diCQA）, 3,4-dicaffeoylquinic acid （3,4-diCQA）, astragalin, kaempferol-3,7-di-O-glucoside and （±）eriodictyol-7-O-β-D-glucuronide. Chromatography was performed on a Kromasil 100-2.5C18 （100 mm×2.1 mm, 2.5 μm） C18 column with gradient elution. The mobile phases consisted of 0.1% formic acid/water （A） and 0.1% formic acid/methanol （B）. The detection wavelength was set at 326 nm and the flow rate was 0.4 mL/min. Ten components were separated well with good linearity （r2〉0.9998）, precision, repeatability, stability. The recovery was in the range of 99.08%-102.77%. The results showed that the content determination using RP-UFLC-DAD fingerprint technique provides an efficient, sensitive, accurate and rapid analytical method for quality assessment ofP. petiolosa （Christ） Ching. Cluster analysis and principal components analysis were successfully applied to analyze 20 samples, the results revealed that the method was efficient and authentic to distinguish producing areas and the source of P. petiolosa （Christ） Ching. Keywords： Pyrrosiapetiolosa （Christ） Ching, Caffeoylquinic acids, Flavonoids, Multicomponent determination, UFLC
文摘A new flavone diglycoside, named as pyrropetioside A 1 was isolated from Pyrrosia petiolosa. Its structrue was elucidated as 7-O-[6-O-(α-L-arabifuranosyl)-β-D-glucopyranosyl]-gossypetin by means of chemical and spectroscopie methods including IR, MS, 1 D and 2D NMR techniques.
基金Supported by National Natural Science Foundation of China(82160773).
文摘[Objectives]The therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed based on the biolabel pattern.[Methods]The chemical components of Folium Pyrrosiae were analyzed by liquid chromatography-mass spectrometry(LC-MS).Ten databases,including Pubchem,CTD,BindingDB,HERB,TCMIP,ETCM,SwissTargetPrediction,SuperPred webserver,TargetNet and SEA,were used in turn to retrieve the targets of related components,and key components were obtained according to the enrichment degree of targets.The obtained targets were imported into the STRING database to obtain PPI information and screen out core targets.The DAVID database was employed to analyze KEGG pathways of core targets and obtain key pathways.A key component-core target-key pathway network of Folium Pyrrosiae was constructed by Cytoscape3.10.1 software.The obtained KEGG pathways were input into the CTD database to predict corresponding diseases,and discussion and analysis were carried out.[Results]Ten key components,30 potential targets and 10 key pathways were screened out,and they participated in many diseases,of which five diseases were mainly analyzed.[Conclusions]Folium Pyrrosiae had the characteristic of multi-component,multi-target and multi-pathway synergistic effect in the treatment of lung cancer,type 2 diabetes,atherosclerosis,liver cancer,prostate cancer and other diseases,and the therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed through the biolabel pattern.This study provides a research basis for further developing new functions of Folium Pyrrosiae.
文摘有柄石韦(Pyrrosia petiolosa)是水龙骨科石韦属植物,也是一种常用的中药材,被广泛用于治疗肾盂肾炎、慢性支气管炎、尿路结石等病症。通过检索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的有柄石韦相关文献,对有柄石韦近四十年的化学成分和生物活性研究进行系统地梳理、归纳,发现目前已从有柄石韦中分离出超过100种化合物,包括黄酮类、三萜类、木脂素类、酚类和其他类等。有柄石韦提取物具有显著的生物活性,包括抗氧化、抗菌、抗炎、利尿、促进伤口愈合、抗肾结石、抗糖尿病等。可为有柄石韦药用资源后续深入系统地研究和临床开发提供科学依据和理论参考。
