Objective: To evaluate the cytotoxic activity of taraxerol isolated from the leaves of Pterospermum acerifolium and its EtOH extract against human breast, colon, and lung cancer cell lines and docking studies.Methods:...Objective: To evaluate the cytotoxic activity of taraxerol isolated from the leaves of Pterospermum acerifolium and its EtOH extract against human breast, colon, and lung cancer cell lines and docking studies.Methods: The structures of the isolated compounds were elucidated by several spectroscopic methods such as 1H-NMR,13C-NMR, DEPT-135, COSY, HSQC, and HMBC. The extract and isolated compound were analyzed for cytotoxic activity on MDA-MB-231, BT-549, A-549, and SW-480 cancer cell lines by MTT assay. Molecular docking was performed on software such as Chem3D pro 12.0.2.1076, Discovery Studio Visualizer, Auto Dock Tools-1.5.6 and Auto dock vina.Results: The extract and isolated compound taraxerol both displayed excellent inhibitory activity(IC50:80 mg/mL for extract and 160 mg/mL for compound) on breast cancer cell(MDA-MB-231). The docking studies show a strong affinity with PI3K(-9.8 Kcal/mol) and m TOR(-10.0 Kcal/mol).Conclusion: The results confirm that the extract and compound exhibited strong cytotoxicity on the MDA-MB-231 cancer cell. So, the extract and the compound may be useful in human chemotherapies.展开更多
翻白叶树是中国南方的常绿树种,具有重要的经济和药用价值。通过Illumina HiSeq X ten高通量测序平台,对翻白叶树叶绿体基因组进行测序、组装和注释,分析翻白叶树的叶绿体基因组特征及系统发育关系。结果表明,翻白叶树叶绿体全基因组具...翻白叶树是中国南方的常绿树种,具有重要的经济和药用价值。通过Illumina HiSeq X ten高通量测序平台,对翻白叶树叶绿体基因组进行测序、组装和注释,分析翻白叶树的叶绿体基因组特征及系统发育关系。结果表明,翻白叶树叶绿体全基因组具有典型的四分体结构,全长为162843 bp,共注释126个基因,其中包括81个蛋白编码基因、37个tRNA和8个rRNA。密码子偏好性分析表明,翻白叶树的密码子使用模式偏向以A或U结尾,亮氨酸(Leu)密码子使用频率最高,半胱氨酸(Cys)使用率最低。重复序列分析共检测到284个SSR位点,其中单碱基重复的SSR位点最多,有171个,6碱基重复最少,仅有2个。翅子树属叶绿体基因组比较分析显示,反向重复区域的位置和边界区域的基因分布存在收缩与扩张的现象。系统发育分析表明,翻白叶树与景东翅子树、勐仑翅子树、截裂翅子树和蚬木亲缘关系较近。该研究为翻白叶树的分子标记开发、物种鉴定以及遗传多样性评估等研究提供重要的理论依据。展开更多
文摘Objective: To evaluate the cytotoxic activity of taraxerol isolated from the leaves of Pterospermum acerifolium and its EtOH extract against human breast, colon, and lung cancer cell lines and docking studies.Methods: The structures of the isolated compounds were elucidated by several spectroscopic methods such as 1H-NMR,13C-NMR, DEPT-135, COSY, HSQC, and HMBC. The extract and isolated compound were analyzed for cytotoxic activity on MDA-MB-231, BT-549, A-549, and SW-480 cancer cell lines by MTT assay. Molecular docking was performed on software such as Chem3D pro 12.0.2.1076, Discovery Studio Visualizer, Auto Dock Tools-1.5.6 and Auto dock vina.Results: The extract and isolated compound taraxerol both displayed excellent inhibitory activity(IC50:80 mg/mL for extract and 160 mg/mL for compound) on breast cancer cell(MDA-MB-231). The docking studies show a strong affinity with PI3K(-9.8 Kcal/mol) and m TOR(-10.0 Kcal/mol).Conclusion: The results confirm that the extract and compound exhibited strong cytotoxicity on the MDA-MB-231 cancer cell. So, the extract and the compound may be useful in human chemotherapies.
文摘翻白叶树是中国南方的常绿树种,具有重要的经济和药用价值。通过Illumina HiSeq X ten高通量测序平台,对翻白叶树叶绿体基因组进行测序、组装和注释,分析翻白叶树的叶绿体基因组特征及系统发育关系。结果表明,翻白叶树叶绿体全基因组具有典型的四分体结构,全长为162843 bp,共注释126个基因,其中包括81个蛋白编码基因、37个tRNA和8个rRNA。密码子偏好性分析表明,翻白叶树的密码子使用模式偏向以A或U结尾,亮氨酸(Leu)密码子使用频率最高,半胱氨酸(Cys)使用率最低。重复序列分析共检测到284个SSR位点,其中单碱基重复的SSR位点最多,有171个,6碱基重复最少,仅有2个。翅子树属叶绿体基因组比较分析显示,反向重复区域的位置和边界区域的基因分布存在收缩与扩张的现象。系统发育分析表明,翻白叶树与景东翅子树、勐仑翅子树、截裂翅子树和蚬木亲缘关系较近。该研究为翻白叶树的分子标记开发、物种鉴定以及遗传多样性评估等研究提供重要的理论依据。
